Restriction map for pF1KE1851
 (Linear) (Six-base) MAPSORT of: KIEE1851.seq  Check: 9857  from: 1  to: 1110



KIEE1851.orf

 With 182 enzymes: SgfI * 

                        July 16, 2019 14:21  ..

AccI GT'mk_AC

Cuts at:      0     869    1110 
   Size:        869     241
 
AfeI AGC'GCT

Cuts at:      0    1030    1110 
   Size:       1030      80
 
AflIII A'CryG_T

Cuts at:      0     454    1110 
   Size:        454     656
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     106     138    1110 
   Size:        106      32     972

  Fragments arranged by size: 

                 972     106      32
 
ApaI G_GGCC'C

Cuts at:      0     329     487    1110 
   Size:        329     158     623

  Fragments arranged by size: 

                 623     329     158
 
AscI GG'CGCG_CC

Cuts at:      0     936    1110 
   Size:        936     174
 
AvaI C'yCGr_G

Cuts at:      0     169     478    1110 
   Size:        169     309     632

  Fragments arranged by size: 

                 632     309     169
 
BamHI G'GATC_C

Cuts at:      0     230    1110 
   Size:        230     880
 
BanI G'GyrC_C

Cuts at:      0     754    1110 
   Size:        754     356
 
BanII G_rGCy'C

Cuts at:      0       8     176     329     487    1110 
   Size:          8     168     153     158     623

  Fragments arranged by size: 

                 623     168     158     153       8
 
BbeI G_GCGC'C

Cuts at:      0     758    1110 
   Size:        758     352
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     836    1110 
   Size:        836     274
 
BglI GCCn_nnn'nGGC

Cuts at:      0     482     539    1110 
   Size:        482      57     571

  Fragments arranged by size: 

                 571     482      57
 
Bme1580I G_kGCm'C

Cuts at:      0     329     487     919    1110 
   Size:        329     158     432     191

  Fragments arranged by size: 

                 432     329     191     158
 
BmgBI CAC'GTC

Cuts at:      0    1062    1110 
   Size:       1062      48
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     170     507    1110 
   Size:        170     337     603

  Fragments arranged by size: 

                 603     337     170
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     305     841    1110 
   Size:        305     536     269

  Fragments arranged by size: 

                 536     305     269
 
BsaAI yAC'GTr

Cuts at:      0     455     692     899    1110 
   Size:        455     237     207     211

  Fragments arranged by size: 

                 455     237     211     207
 
BsaHI Gr'CG_yC

Cuts at:      0     207     755     909    1110 
   Size:        207     548     154     201

  Fragments arranged by size: 

                 548     207     201     154
 
BsaWI w'CCGG_w

Cuts at:      0     290    1110 
   Size:        290     820
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     106     136     640     670    1110 
   Size:        106      30     504      30     440

  Fragments arranged by size: 

                 504     440     106      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     114    1110 
   Size:        114     996
 
BseYI C'CCAG_C

Cuts at:      0      26    1110 
   Size:         26    1084
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     220     435     879    1023    1062    1081    1110 
   Size:        220     215     444     144      39      19      29

  Fragments arranged by size: 

                 444     220     215     144      39      29      19
 
BsiEI CG_ry'CG

Cuts at:      0     531     543     626     744    1110 
   Size:        531      12      83     118     366

  Fragments arranged by size: 

                 531     366     118      83      12
 
BsiWI C'GTAC_G

Cuts at:      0     619    1110 
   Size:        619     491
 
Bsp1286I G_dGCh'C

Cuts at:      0       8     176     329     487     919    1110 
   Size:          8     168     153     158     432     191

  Fragments arranged by size: 

                 432     191     168     158     153       8
 
BspEI T'CCGG_A

Cuts at:      0     290    1110 
   Size:        290     820
 
BspHI T'CATG_A

Cuts at:      0     590    1110 
   Size:        590     520
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     143     587    1110 
   Size:        143     444     523

  Fragments arranged by size: 

                 523     444     143
 
BsrBI CCG'CTC

Cuts at:      0      61    1110 
   Size:         61    1049
 
BsrFI r'CCGG_y

Cuts at:      0     464    1110 
   Size:        464     646
 
BssHII G'CGCG_C

Cuts at:      0     418     717     936    1110 
   Size:        418     299     219     174

  Fragments arranged by size: 

                 418     299     219     174
 
BssSI C'ACGA_G

Cuts at:      0    1099    1110 
   Size:       1099      11
 
BstEII G'GTnAC_C

Cuts at:      0     402    1110 
   Size:        402     708
 
BstYI r'GATC_y

Cuts at:      0     230    1110 
   Size:        230     880
 
BtgI C'CryG_G

Cuts at:      0     660     722     780    1110 
   Size:        660      62      58     330

  Fragments arranged by size: 

                 660     330      62      58
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     743    1110 
   Size:        743     367
 
BtsI GCAGTG_nn'

Cuts at:      0      96     314    1110 
   Size:         96     218     796

  Fragments arranged by size: 

                 796     218      96
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      72     107    1110 
   Size:         72      35    1003

  Fragments arranged by size: 

                1003      72      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     243    1110 
   Size:        243     867
 
EaeI y'GGCC_r

Cuts at:      0      56      93     528     540    1110 
   Size:         56      37     435      12     570

  Fragments arranged by size: 

                 570     435      56      37      12
 
EagI C'GGCC_G

Cuts at:      0     528     540    1110 
   Size:        528      12     570

  Fragments arranged by size: 

                 570     528      12
 
EarI CTCTTCn'nnn_

Cuts at:      0     340     541    1110 
   Size:        340     201     569

  Fragments arranged by size: 

                 569     340     201
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     384     820    1110 
   Size:        384     436     290

  Fragments arranged by size: 

                 436     384     290
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     305     841    1110 
   Size:        305     536     269

  Fragments arranged by size: 

                 536     305     269
 
EcoO109I rG'GnC_Cy

Cuts at:      0     325     326     483     484     576    1110 
   Size:        325       1     157       1      92     534

  Fragments arranged by size: 

                 534     325     157      92       1       1
 
FspI TGC'GCA

Cuts at:      0     688    1110 
   Size:        688     422
 
FspAI rTGC'GCAy

Cuts at:      0     688    1110 
   Size:        688     422
 
HaeII r_GCGC'y

Cuts at:      0      16      55     758    1032    1110 
   Size:         16      39     703     274      78

  Fragments arranged by size: 

                 703     274      78      39      16
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     742     774    1110 
   Size:        742      32     336

  Fragments arranged by size: 

                 742     336      32
 
HincII GTy'rAC

Cuts at:      0     351    1110 
   Size:        351     759
 
KasI G'GCGC_C

Cuts at:      0     754    1110 
   Size:        754     356
 
MspA1I CmG'CkG

Cuts at:      0     186     204     302     782     905    1067    1110 
   Size:        186      18      98     480     123     162      43

  Fragments arranged by size: 

                 480     186     162     123      98      43      18
 
NarI GG'CG_CC

Cuts at:      0     755    1110 
   Size:        755     355
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     414    1110 
   Size:        414     696
 
PmlI CAC'GTG

Cuts at:      0     455     692    1110 
   Size:        455     237     418

  Fragments arranged by size: 

                 455     418     237
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     106     138    1110 
   Size:        106      32     972

  Fragments arranged by size: 

                 972     106      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     576    1110 
   Size:        576     534
 
PspOMI G'GGCC_C

Cuts at:      0     325     483    1110 
   Size:        325     158     627

  Fragments arranged by size: 

                 627     325     158
 
PspXI vC'TCGA_Gb

Cuts at:      0     169    1110 
   Size:        169     941
 
PstI C_TGCA'G

Cuts at:      0     185     260     511    1110 
   Size:        185      75     251     599

  Fragments arranged by size: 

                 599     251     185      75
 
PvuII CAG'CTG

Cuts at:      0     186    1067    1110 
   Size:        186     881      43

  Fragments arranged by size: 

                 881     186      43
 
SacII CC_GC'GG

Cuts at:      0     783    1110 
   Size:        783     327
 
SfcI C'TryA_G

Cuts at:      0     181     256     507    1110 
   Size:        181      75     251     603

  Fragments arranged by size: 

                 603     251     181      75
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     482    1110 
   Size:        482     628
 
SfoI GGC'GCC

Cuts at:      0     756    1110 
   Size:        756     354
 
SmaI CCC'GGG

Cuts at:      0     480    1110 
   Size:        480     630
 
SmlI C'TyrA_G

Cuts at:      0     169    1110 
   Size:        169     941
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     421    1110 
   Size:        421     689
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     640     652     672     684    1110 
   Size:        640      12      20      12     426

  Fragments arranged by size: 

                 640     426      20      12      12
 
Tth111I GACn'n_nGTC

Cuts at:      0     865    1110 
   Size:        865     245
 
XhoI C'TCGA_G

Cuts at:      0     169    1110 
   Size:        169     941
 
XmaI C'CCGG_G

Cuts at:      0     478    1110 
   Size:        478     632
 

 Enzymes that do cut:

     AccI     AfeI   AflIII     AloI     ApaI     AscI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI     BglI Bme1580I    BmgBI     BmrI
     BpmI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI    BsiWI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrFI
   BssHII    BssSI   BstEII    BstYI     BtgI    BtgZI     BtsI    CspCI
   DraIII     EaeI     EagI     EarI     EciI  Eco57MI EcoO109I     FspI
    FspAI    HaeII    Hin4I   HincII     KasI   MspA1I     NarI    PflMI
     PmlI     PpiI    PpuMI   PspOMI    PspXI     PstI    PvuII    SacII
     SfcI     SfiI     SfoI     SmaI     SmlI    TaqII     TstI  Tth111I
     XhoI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI    AflII     AgeI     AhdI
     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI    AvrII
     BaeI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaBI  BsiHKAI     BsmI
    BsmBI    BsrDI    BsrGI   BstAPI    BstBI    BstXI  BstZ17I   Bsu36I
     ClaI     DraI     DrdI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI
     FseI  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI
     MslI     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI    PshAI
     PsiI     PsrI     PvuI    RsrII     SacI     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SgfI    SgrAI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI     TatI     XbaI     XcmI
     XmnI     ZraI
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