Restriction map for pF1KE3824
##################################
# NO CUT ENZYMES (   63 enzymes) #
##################################

AatII       Acc65I      AccIII      AgeI        ApaI        
AscI        Asp718I     AspI        AviII       AvrII       
BamHI       BbrPI       BbvCI       BcgI        BglI        
BlnI        BsaAI       BseAI       BsiWI       BspDI       
BspEI       BsrBI       BssHII      Bst1107I    BstAPI      
BstEII      BstPI       BstZ17I     ClaI        CpoI        
EagI        EclXI       Eco52I      EcoNI       EcoO65I     
FseI        FspI        KpnI        MluI        MroI        
NaeI        NdeI        NgoMIV      NheI        NotI        
NruI        PacI        PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PvuI        RsrII       
SalI        SexAI       SfiI        SgrAI       SnaBI       
SwaI        Tth111I     XbaI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AccI         5224  GTAGAC               GT^[AC][GT]AC
AccII        2644  CGCG                 CG^CG
AclI          742  AACGTT               AA^CGTT
AflII        6447  CTTAAG               C^TTAAG
AhdI         2408  GACTACAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI        6583  CAGCTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
AspEI        2408  GACTACAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanII        2797  GAGCTC               G[GA]GC[CT]^C
BbeI         5819  GGCGCC               GGCGC^C
BfrI         6447  CTTAAG               C^TTAAG
BsaBI        5900  GATATATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI         6326  GTGCAG               GTGCAG(16/14)
BsiEI          26  CGGTCG               CG[GA][CT]^CG
BsmBI        4270  GAGACG               CGTCTC(1/5)
Bsp1407I     1344  TGTACA               T^GTACA
BspHI        2604  TCATGA               T^CATGA
BspLU11I     6052  ACATGT               A^CATGT
BsrGI        1344  TGTACA               T^GTACA
BssSI          14  CTCGTG               CACGAG(-5/-1)
BstBI        2543  TTCGAA               TT^CGAA
BstUI        2644  CGCG                 CG^CG
DrdI         6422  GACCTTCTAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     2408  GACTACAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HgaI         6516  GCGTC                GACGC(5/10)
HincII       2193  GTTAAC               GT[CT]^[GA]AC
HindII       2193  GTTAAC               GT[CT]^[GA]AC
HpaI         2193  GTTAAC               GTT^AAC
KasI         5819  GGCGCC               G^GCGCC
KspI         2643  CCGCGG               CCGC^GG
MamI         5900  GATATATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI         2644  CGCG                 CG^CG
NarI         5819  GGCGCC               GG^CGCC
NspV         2543  TTCGAA               TT^CGAA
PaeR7I       5456  CTCGAG               C^TCGAG
PpuMI        6642  AGGTCCT              [GA]G^G[AT]CC[CT]
Psp1406I      742  AACGTT               AA^CGTT
RcaI         2604  TCATGA               T^CATGA
SacI         2797  GAGCTC               GAGCT^C
SacII        2643  CCGCGG               CCGC^GG
SapI         2933  GCTCTTC              GCTCTTC(1/4)
SbfI         7212  CCTGCAGG             CCTGCA^GG
SfoI         5819  GGCGCC               GGC^GCC
SfuI         2543  TTCGAA               TT^CGAA
SmaI         6082  CCCGGG               CCC^GGG
SpeI         2259  ACTAGT               A^CTAGT
SphI         3635  GCATGC               GCATG^C
Sse8387I     7212  CCTGCAGG             CCTGCA^GG
SspBI        1344  TGTACA               T^GTACA
StuI          241  AGGCCT               AGG^CCT
XhoI         5456  CTCGAG               C^TCGAG
XmaCI        6082  CCCGGG               C^CCGGG
XmaI         6082  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AcyI         5819  GGCGCC               G[GA]^CG[CT]C
             6515  GGCGTC              
AflIII        568  ACACGT               A^C[GA][CT]GT
             6052  ACATGT              
Alw44I       3308  GTGCAC               G^TGCAC
             4017  GTGCAC              
AocI         1123  CCTGAGG              CC^T[ACGT]AGG
             2023  CCTCAGG             
Aor51HI      5345  AGCGCT               AGC^GCT
             7121  AGCGCT              
ApaLI        3308  GTGCAC               G^TGCAC
             4017  GTGCAC              
AvaI         5456  CTCGAG               C^[CT]CG[GA]G
             6082  CCCGGG              
BanI         3436  GGTGCC               G^G[CT][GA]CC
             5819  GGCGCC              
BciVI        1166  GGATAC               GTATCC(6/5)
             7636  GTATCC              
BlpI         2048  GCTTAGC              GC^T[ACGT]AGC
             3664  GCTCAGC             
Bpu1102I     2048  GCTTAGC              GC^T[ACGT]AGC
             3664  GCTCAGC             
BsaHI        5819  GGCGCC               G[GA]^CG[CT]C
             6515  GGCGTC              
BsaWI          24  TCCGGT               [AT]^CCGG[AT]
             4467  TCCGGT              
BsoBI        5456  CTCGAG               C^[CT]CG[GA]G
             6082  CCCGGG              
BsrFI        4040  ACCGGC               [GA]^CCGG[CT]
             4684  GCCGGT              
Bsu36I       1123  CCTGAGG              CC^T[ACGT]AGG
             2023  CCTCAGG             
CelII        2048  GCTTAGC              GC^T[ACGT]AGC
             3664  GCTCAGC             
Cfr10I       4040  ACCGGC               [GA]^CCGG[CT]
             4684  GCCGGT              
DraI          690  TTTAAA               TTT^AAA
             5100  TTTAAA              
DraII         320  AGGGCCT              [GA]G^G[ACGT]CC[CT]
             6642  AGGTCCT             
Eco47III     5345  AGCGCT               AGC^GCT
             7121  AGCGCT              
Eco81I       1123  CCTGAGG              CC^T[ACGT]AGG
             2023  CCTCAGG             
EcoO109I      320  AGGGCCT              [GA]G^G[ACGT]CC[CT]
             6642  AGGTCCT             
FauI         1702  CCCGC                CCCGC(4/6)
             4649  CCCGC               
Hin1I        5819  GGCGCC               G[GA]^CG[CT]C
             6515  GGCGTC              
MfeI         2732  CAATTG               C^AATTG
             7320  CAATTG              
MunI         2732  CAATTG               C^AATTG
             7320  CAATTG              
NcoI          524  CCATGG               C^CATGG
             2967  CCATGG              
SspI          996  AATATT               AAT^ATT
             4168  AATATT              


#########################################
# 3 OR MORE CUT ENZYMES (  114 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AseI        AsnI        Asp700I     AspHI       
AvaII       BalI        BbsI        BbvI        BclI        
BcnI        BfaI        BglII       BmyI        BpmI        
BpuAI       BsaI        BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsmI        
Bsp1286I    BspMI       BsrDI       BsrI        BssKI       
BstF5I      BstNI       BstXI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraIII      
DsaI        EaeI        EarI        EcoRI       EcoRII      
EcoRV       EcoT14I     EcoT22I     FbaI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
HinP1I      HindIII     HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NsiI        NspI        PflMI       PleI        
PshBI       PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScaI        ScrFI       SfaNI       SfcI        
SmlI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XcmI        XhoII       XmnI        
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