Restriction map for pF1KE3824
 (Linear) (Six-base) MAPSORT of: KIEE3824.seq  Check: 8543  from: 1  to: 7662



KIEE3824.orf

 With 182 enzymes: SgfI * 

                        November 2, 2012 16:50  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    5479    7662 
   Size:       5479    2183
 
AccI GT'mk_AC

Cuts at:      0    5225    7662 
   Size:       5225    2437
 
AclI AA'CG_TT

Cuts at:      0     743    7662 
   Size:        743    6919
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     629    2158    2488    3233    3589    4208    4919    4924 
   Size:        629    1529     330     745     356     619     711       5

Cuts at:   4924    6125    7499    7662 
   Size:       1201    1374     163

  Fragments arranged by size: 

                1529    1374    1201     745     711     629     619     356
                 330     163       5
 
AfeI AGC'GCT

Cuts at:      0    5347    7123    7662 
   Size:       5347    1776     539

  Fragments arranged by size: 

                5347    1776     539
 
AflII C'TTAA_G

Cuts at:      0    6447    7662 
   Size:       6447    1215
 
AflIII A'CryG_T

Cuts at:      0     568    6052    7662 
   Size:        568    5484    1610

  Fragments arranged by size: 

                5484    1610     568
 
AhdI GACnn_n'nnGTC

Cuts at:      0    2413    7662 
   Size:       2413    5249
 
AlwNI CAG_nnn'CTG

Cuts at:      0    6588    7662 
   Size:       6588    1074
 
ApaLI G'TGCA_C

Cuts at:      0    3308    4017    7662 
   Size:       3308     709    3645

  Fragments arranged by size: 

                3645    3308     709
 
ApoI r'AATT_y

Cuts at:      0     123     389     428     547     679     945    1031    1724 
   Size:        123     266      39     119     132     266      86     693

Cuts at:   1724    1755    3797    4336    4704    5062    5449    5695    7061 
   Size:         31    2042     539     368     358     387     246    1366

Cuts at:   7061    7082    7662 
   Size:         21     580

  Fragments arranged by size: 

                2042    1366     693     580     539     387     368     358
                 266     266     246     132     123     119      86      39
                  31      21
 
AseI AT'TA_AT

Cuts at:      0     725    2144    4172    4384    4493    7662 
   Size:        725    1419    2028     212     109    3169

  Fragments arranged by size: 

                3169    2028    1419     725     212     109
 
AvaI C'yCGr_G

Cuts at:      0    5456    6082    7662 
   Size:       5456     626    1580

  Fragments arranged by size: 

                5456    1580     626
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    5181    5214    7662 
   Size:       5181      33    2448

  Fragments arranged by size: 

                5181    2448      33
 
BanI G'GyrC_C

Cuts at:      0    3436    5819    7662 
   Size:       3436    2383    1843

  Fragments arranged by size: 

                3436    2383    1843
 
BanII G_rGCy'C

Cuts at:      0    2801    7662 
   Size:       2801    4861
 
BbeI G_GCGC'C

Cuts at:      0    5823    7662 
   Size:       5823    1839
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1201    2444    3814    3845    6583    6731    6878    7290 
   Size:       1201    1243    1370      31    2738     148     147     412

Cuts at:   7290    7662 
   Size:        372

  Fragments arranged by size: 

                2738    1370    1243    1201     412     372     148     147
                  31
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1160    7647    7662 
   Size:       1160    6487      15

  Fragments arranged by size: 

                6487    1160      15
 
BclI T'GATC_A

Cuts at:      0     233    1395    3576    5157    5790    6125    6459    7662 
   Size:        233    1162    2181    1581     633     335     334    1203

  Fragments arranged by size: 

                2181    1581    1203    1162     633     335     334     233
            
 
BfrBI ATG'CAT

Cuts at:      0    5302    5997    6217    7662 
   Size:       5302     695     220    1445

  Fragments arranged by size: 

                5302    1445     695     220
 
BglII A'GATC_T

Cuts at:      0    2370    5165    7227    7662 
   Size:       2370    2795    2062     435

  Fragments arranged by size: 

                2795    2370    2062     435
 
BlpI GC'TnA_GC

Cuts at:      0    2049    3665    7662 
   Size:       2049    1616    3997

  Fragments arranged by size: 

                3997    2049    1616
 
Bme1580I G_kGCm'C

Cuts at:      0    1593    3312    3603    4021    4991    6322    7662 
   Size:       1593    1719     291     418     970    1331    1340

  Fragments arranged by size: 

                1719    1593    1340    1331     970     418     291
 
BmgBI CAC'GTC

Cuts at:      0     571    7662 
   Size:        571    7091
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    5418    7327    7662 
   Size:       5418    1909     335

  Fragments arranged by size: 

                5418    1909     335
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1784    7662 
   Size:       1784    5878
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     617    3041    6334    7436    7662 
   Size:        617    2424    3293    1102     226

  Fragments arranged by size: 

                3293    2424    1102     617     226
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     576    1483    6474    6510    7662 
   Size:        576     907    4991      36    1152

  Fragments arranged by size: 

                4991    1152     907     576      36
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1889    2720    7331    7662 
   Size:       1889     831    4611     331

  Fragments arranged by size: 

                4611    1889     831     331
 
BsaBI GATnn'nnATC

Cuts at:      0    5904    7662 
   Size:       5904    1758
 
BsaHI Gr'CG_yC

Cuts at:      0    5820    6516    7662 
   Size:       5820     696    1146

  Fragments arranged by size: 

                5820    1146     696
 
BsaWI w'CCGG_w

Cuts at:      0      24    4467    7662 
   Size:         24    4443    3195

  Fragments arranged by size: 

                4443    3195      24
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1120    1150    1337    1367    1603    1633    2698    2728 
   Size:       1120      30     187      30     236      30    1065      30

Cuts at:   2728    2940    2970    3107    3137    7662 
   Size:        212      30     137      30    4525

  Fragments arranged by size: 

                4525    1120    1065     236     212     187     137      30
                  30      30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1141    2012    2365    6068    6749    7441    7444    7662 
   Size:       1141     871     353    3703     681     692       3     218

  Fragments arranged by size: 

                3703    1141     871     692     681     353     218       3
            
 
BseYI C'CCAG_C

Cuts at:      0    1929    6920    7560    7662 
   Size:       1929    4991     640     102

  Fragments arranged by size: 

                4991    1929     640     102
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    6347    7662 
   Size:       6347    1315
 
BsiEI CG_ry'CG

Cuts at:      0      29    7662 
   Size:         29    7633
 
BsiHKAI G_wGCw'C

Cuts at:      0    2022    2801    3312    4021    4090    5606    7608    7662 
   Size:       2022     779     511     709      69    1516    2002      54

  Fragments arranged by size: 

                2022    2002    1516     779     709     511      69      54
            
 
BsmI GAATG_Cn'

Cuts at:      0    1062    2064    2638    3417    3611    5844    6011    6737 
   Size:       1062    1002     574     779     194    2233     167     726

Cuts at:   6737    7662 
   Size:        925

  Fragments arranged by size: 

                2233    1062    1002     925     779     726     574     194
                 167
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    4264    7662 
   Size:       4264    3398
 
Bsp1286I G_dGCh'C

Cuts at:      0    1593    2022    2801    3312    3603    4021    4090    4991 
   Size:       1593     429     779     511     291     418      69     901

Cuts at:   4991    5606    6322    7608    7662 
   Size:        615     716    1286      54

  Fragments arranged by size: 

                1593    1286     901     779     716     615     511     429
                 418     291      69      54
 
BspHI T'CATG_A

Cuts at:      0    2604    7662 
   Size:       2604    5058
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     201    5479    6612    6960    7662 
   Size:        201    5278    1133     348     702

  Fragments arranged by size: 

                5278    1133     702     348     201
 
BsrDI GCAATG_nn'

Cuts at:      0     540     787    3828    4833    6723    7227    7350    7662 
   Size:        540     247    3041    1005    1890     504     123     312

  Fragments arranged by size: 

                3041    1890    1005     540     504     312     247     123
            
 
BsrFI r'CCGG_y

Cuts at:      0    4040    4684    7662 
   Size:       4040     644    2978

  Fragments arranged by size: 

                4040    2978     644
 
BsrGI T'GTAC_A

Cuts at:      0    1344    7662 
   Size:       1344    6318
 
BssSI C'ACGA_G

Cuts at:      0      14    7662 
   Size:         14    7648
 
BstBI TT'CG_AA

Cuts at:      0    2544    7662 
   Size:       2544    5118
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     656    2112    5662    5761    7507    7540    7662 
   Size:        656    1456    3550      99    1746      33     122

  Fragments arranged by size: 

                3550    1746    1456     656     122      99      33
 
BstYI r'GATC_y

Cuts at:      0     636     666    1287    1643    2133    2370    2823    2953 
   Size:        636      30     621     356     490     237     453     130

Cuts at:   2953    5165    7011    7227    7510    7662 
   Size:       2212    1846     216     283     152

  Fragments arranged by size: 

                2212    1846     636     621     490     453     356     283
                 237     216     152     130      30
 
Bsu36I CC'TnA_GG

Cuts at:      0    1124    2024    7662 
   Size:       1124     900    5638

  Fragments arranged by size: 

                5638    1124     900
 
BtgI C'CryG_G

Cuts at:      0     524    2643    2967    6189    7662 
   Size:        524    2119     324    3222    1473

  Fragments arranged by size: 

                3222    2119    1473     524     324
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      86    7662 
   Size:         86    7576
 
BtsI GCAGTG_nn'

Cuts at:      0     413    3034    3590    7259    7632    7662 
   Size:        413    2621     556    3669     373      30

  Fragments arranged by size: 

                3669    2621     556     413     373      30
 
DraI TTT'AAA

Cuts at:      0     692    5102    7662 
   Size:        692    4410    2560

  Fragments arranged by size: 

                4410    2560     692
 
DraIII CAC_nnn'GTG

Cuts at:      0    1518    2489    7384    7662 
   Size:       1518     971    4895     278

  Fragments arranged by size: 

                4895    1518     971     278
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    6428    7662 
   Size:       6428    1234
 
EaeI y'GGCC_r

Cuts at:      0     215     479    7596    7662 
   Size:        215     264    7117      66

  Fragments arranged by size: 

                7117     264     215      66
 
EarI CTCTTCn'nnn_

Cuts at:      0     806    1127    2940    4262    6729    6767    7662 
   Size:        806     321    1813    1322    2467      38     895

  Fragments arranged by size: 

                2467    1813    1322     895     806     321      38
 
EcoICRI GAG'CTC

Cuts at:      0    2799    7662 
   Size:       2799    4863
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     617     629    2158    2488    3041    3233    3589    4208 
   Size:        617      12    1529     330     553     192     356     619

Cuts at:   4208    4919    4924    6125    6334    7436    7499    7662 
   Size:        711       5    1201     209    1102      63     163

  Fragments arranged by size: 

                1529    1201    1102     711     619     617     553     356
                 330     209     192     163      63      12       5
 
EcoO109I rG'GnC_Cy

Cuts at:      0     321    6643    7662 
   Size:        321    6322    1019

  Fragments arranged by size: 

                6322    1019     321
 
EcoRI G'AATT_C

Cuts at:      0    4704    7061    7082    7662 
   Size:       4704    2357      21     580

  Fragments arranged by size: 

                4704    2357     580      21
 
EcoRV GAT'ATC

Cuts at:      0    1085    1569    3674    4858    7662 
   Size:       1085     484    2105    1184    2804

  Fragments arranged by size: 

                2804    2105    1184    1085     484
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1987    2172    7662 
   Size:       1987     185    5490

  Fragments arranged by size: 

                5490    1987     185
 
HaeII r_GCGC'y

Cuts at:      0    1750    5349    5823    7125    7662 
   Size:       1750    3599     474    1302     537

  Fragments arranged by size: 

                3599    1750    1302     537     474
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      52      84     628     660    1461    1493    2399    2431 
   Size:         52      32     544      32     801      32     906      32

Cuts at:   2431    2698    2730    2741    2773    3313    3345    4780    4812 
   Size:        267      32      11      32     540      32    1435      32

Cuts at:   4812    7662 
   Size:       2850

  Fragments arranged by size: 

                2850    1435     906     801     544     540     267      52
                  32      32      32      32      32      32      32      32
                  11
 
HincII GTy'rAC

Cuts at:      0    2195    7662 
   Size:       2195    5467
 
HindIII A'AGCT_T

Cuts at:      0    3466    3515    5096    5105    6450    7406    7662 
   Size:       3466      49    1581       9    1345     956     256

  Fragments arranged by size: 

                3466    1581    1345     956     256      49       9
 
HpaI GTT'AAC

Cuts at:      0    2195    7662 
   Size:       2195    5467
 
KasI G'GCGC_C

Cuts at:      0    5819    7662 
   Size:       5819    1843
 
MfeI C'AATT_G

Cuts at:      0    2732    7320    7662 
   Size:       2732    4588     342

  Fragments arranged by size: 

                4588    2732     342
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0       9     108     203    1405    3922    4623    7662 
   Size:          9      99      95    1202    2517     701    3039

  Fragments arranged by size: 

                3039    2517    1202     701      99      95       9
 
MscI TGG'CCA

Cuts at:      0     217     481    7598    7662 
   Size:        217     264    7117      64

  Fragments arranged by size: 

                7117     264     217      64
 
MslI CAynn'nnrTG

Cuts at:      0     573    1112    1550    1826    2723    2981    3630    3747 
   Size:        573     539     438     276     897     258     649     117

Cuts at:   3747    7155    7538    7662 
   Size:       3408     383     124

  Fragments arranged by size: 

                3408     897     649     573     539     438     383     276
                 258     124     117
 
MspA1I CmG'CkG

Cuts at:      0    1147    2007    2645    4453    6702    6710    7662 
   Size:       1147     860     638    1808    2249       8     952

  Fragments arranged by size: 

                2249    1808    1147     952     860     638       8
 
NarI GG'CG_CC

Cuts at:      0    5820    7662 
   Size:       5820    1842
 
NcoI C'CATG_G

Cuts at:      0     524    2967    7662 
   Size:        524    2443    4695

  Fragments arranged by size: 

                4695    2443     524
 
NsiI A_TGCA'T

Cuts at:      0    5304    5999    6219    7662 
   Size:       5304     695     220    1443

  Fragments arranged by size: 

                5304    1443     695     220
 
NspI r_CATG'y

Cuts at:      0     347    3639    6056    7662 
   Size:        347    3292    2417    1606

  Fragments arranged by size: 

                3292    2417    1606     347
 
PasI CC'CwG_GG

Cuts at:      0    4838    7662 
   Size:       4838    2824
 
PciI A'CATG_T

Cuts at:      0    6052    7662 
   Size:       6052    1610
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    4642    6206    7294    7662 
   Size:       4642    1564    1088     368

  Fragments arranged by size: 

                4642    1564    1088     368
 
PfoI T'CCnGG_A

Cuts at:      0     963    2958    6417    7662 
   Size:        963    1995    3459    1245

  Fragments arranged by size: 

                3459    1995    1245     963
 
PpuMI rG'GwC_Cy

Cuts at:      0    6643    7662 
   Size:       6643    1019
 
PstI C_TGCA'G

Cuts at:      0    2832    6880    7217    7662 
   Size:       2832    4048     337     445

  Fragments arranged by size: 

                4048    2832     445     337
 
PvuII CAG'CTG

Cuts at:      0    1147    2007    4453    6702    7662 
   Size:       1147     860    2446    2249     960

  Fragments arranged by size: 

                2446    2249    1147     960     860
 
SacI G_AGCT'C

Cuts at:      0    2801    7662 
   Size:       2801    4861
 
SacII CC_GC'GG

Cuts at:      0    2646    7662 
   Size:       2646    5016
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2940    7662 
   Size:       2940    4722
 
SbfI CC_TGCA'GG

Cuts at:      0    7217    7662 
   Size:       7217     445
 
ScaI AGT'ACT

Cuts at:      0     820    3125    6479    7662 
   Size:        820    2305    3354    1183

  Fragments arranged by size: 

                3354    2305    1183     820
 
SfcI C'TryA_G

Cuts at:      0    1562    2366    2828    4423    6161    6876    7213    7662 
   Size:       1562     804     462    1595    1738     715     337     449

  Fragments arranged by size: 

                1738    1595    1562     804     715     462     449     337
            
 
SfoI GGC'GCC

Cuts at:      0    5821    7662 
   Size:       5821    1841
 
SmaI CCC'GGG

Cuts at:      0    6084    7662 
   Size:       6084    1578
 
SmlI C'TyrA_G

Cuts at:      0     591    1462    5456    6447    6453    6489    7662 
   Size:        591     871    3994     991       6      36    1173

  Fragments arranged by size: 

                3994    1173     991     871     591      36       6
 
SpeI A'CTAG_T

Cuts at:      0    2259    7662 
   Size:       2259    5403
 
SphI G_CATG'C

Cuts at:      0    3639    7662 
   Size:       3639    4023
 
SspI AAT'ATT

Cuts at:      0     998    4170    7662 
   Size:        998    3172    3492

  Fragments arranged by size: 

                3492    3172     998
 
StuI AGG'CCT

Cuts at:      0     243    7662 
   Size:        243    7419
 
StyI C'CwwG_G

Cuts at:      0     524    2397    2967    4290    5172    7652    7662 
   Size:        524    1873     570    1323     882    2480      10

  Fragments arranged by size: 

                2480    1873    1323     882     570     524      10
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     240    4671    5587    7109    7662 
   Size:        240    4431     916    1522     553

  Fragments arranged by size: 

                4431    1522     916     553     240
 
TatI w'GTAC_w

Cuts at:      0     818    1344    3123    3375    4913    5771    6011    6477 
   Size:        818     526    1779     252    1538     858     240     466

Cuts at:   6477    7307    7662 
   Size:        830     355

  Fragments arranged by size: 

                1779    1538     858     830     818     526     466     355
                 252     240
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1120    1152    1628    1660    2940    2972    6689    6721 
   Size:       1120      32     476      32    1280      32    3717      32

Cuts at:   6721    7662 
   Size:        941

  Fragments arranged by size: 

                3717    1280    1120     941     476      32      32      32
                  32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      58     559    3000    3196    4029    4665    5662    5761 
   Size:         58     501    2441     196     833     636     997      99

Cuts at:   5761    6564    7662 
   Size:        803    1098

  Fragments arranged by size: 

                2441    1098     997     833     803     636     501     196
                  99      58
 
XhoI C'TCGA_G

Cuts at:      0    5456    7662 
   Size:       5456    2206
 
XmaI C'CCGG_G

Cuts at:      0    6082    7662 
   Size:       6082    1580
 
XmnI GAAnn'nnTTC

Cuts at:      0     842    1673    2540    4193    5008    6308    7662 
   Size:        842     831     867    1653     815    1300    1354

  Fragments arranged by size: 

                1653    1354    1300     867     842     831     815
 

 Enzymes that do cut:

     AarI     AccI     AclI     AcuI     AfeI    AflII   AflIII     AhdI
    AlwNI    ApaLI     ApoI     AseI     AvaI     BaeI     BanI    BanII
     BbeI     BbsI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BplI     BpmI    BpuEI     BsaI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspHI    BspMI    BsrDI    BsrFI    BsrGI    BssSI
    BstBI    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DraI
   DraIII     DrdI     EaeI     EarI  EcoICRI  Eco57MI EcoO109I    EcoRI
    EcoRV     FalI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     MfeI     MmeI     MscI     MslI   MspA1I     NarI     NcoI     NsiI
     NspI     PasI     PciI    PflMI     PfoI    PpuMI     PstI    PvuII
     SacI    SacII     SapI     SbfI     ScaI     SfcI     SfoI     SmaI
     SmlI     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI
     TstI     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AgeI     AleI     AlfI     AloI     ApaI     AscI
    AsiSI    AvrII    BamHI    BbvCI     BcgI     BglI     BmtI   Bpu10I
    BsaAI    BsiWI    BspEI    BsrBI   BssHII   BstAPI   BstEII  BstZ17I
     ClaI    CspCI     EagI     EciI    EcoNI     FseI     FspI    FspAI
     KpnI     MluI     NaeI     NdeI   NgoMIV     NheI     NotI     NruI
     PacI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII     SalI    SanDI    SexAI     SfiI     SgfI
    SgrAI    SnaBI     SrfI     SwaI  Tth111I     XbaI     ZraI
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