Restriction map for pF1KE3388
 (Linear) (Six-base) MAPSORT of: KIEE3388.seq  Check: 6752  from: 1  to: 3642



KIEE3388.orf

 With 182 enzymes: SgfI * 

                        October 12, 2011 16:54  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2733    3642 
   Size:       2733     909
 
Acc65I G'GTAC_C

Cuts at:      0    2563    3642 
   Size:       2563    1079
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1386    1742    1867    2488    2866    3117    3642 
   Size:       1386     356     125     621     378     251     525

  Fragments arranged by size: 

                1386     621     525     378     356     251     125
 
AfeI AGC'GCT

Cuts at:      0     610    3642 
   Size:        610    3032
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1665    3642 
   Size:       1665    1977
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     793    1339    1800    3642 
   Size:        793     546     461    1842

  Fragments arranged by size: 

                1842     793     546     461
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    3407    3439    3642 
   Size:       3407      32     203

  Fragments arranged by size: 

                3407     203      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1748    2814    3024    3476    3642 
   Size:       1748    1066     210     452     166

  Fragments arranged by size: 

                1748    1066     452     210     166
 
ApaLI G'TGCA_C

Cuts at:      0     744    3642 
   Size:        744    2898
 
ApoI r'AATT_y

Cuts at:      0     592    3346    3642 
   Size:        592    2754     296

  Fragments arranged by size: 

                2754     592     296
 
AseI AT'TA_AT

Cuts at:      0    2228    3642 
   Size:       2228    1414
 
AvaI C'yCGr_G

Cuts at:      0      61      85    1238    1814    2657    3642 
   Size:         61      24    1153     576     843     985

  Fragments arranged by size: 

                1153     985     843     576      61      24
 
AvrII C'CTAG_G

Cuts at:      0    2085    3642 
   Size:       2085    1557
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     489     522    3642 
   Size:        489      33    3120

  Fragments arranged by size: 

                3120     489      33
 
BamHI G'GATC_C

Cuts at:      0    3192    3590    3642 
   Size:       3192     398      52

  Fragments arranged by size: 

                3192     398      52
 
BanI G'GyrC_C

Cuts at:      0      16    2563    3642 
   Size:         16    2547    1079

  Fragments arranged by size: 

                2547    1079      16
 
BanII G_rGCy'C

Cuts at:      0      86      98     455    1258    1585    3098    3141    3293 
   Size:         86      12     357     803     327    1513      43     152

Cuts at:   3293    3420    3520    3527    3642 
   Size:        127     100       7     115

  Fragments arranged by size: 

                1513     803     357     327     152     127     115     100
                  86      43      12       7
 
BbeI G_GCGC'C

Cuts at:      0      20    3642 
   Size:         20    3622
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     160     963    1044    1403    1448    1576    2494    2864 
   Size:        160     803      81     359      45     128     918     370

Cuts at:   2864    3642 
   Size:        778

  Fragments arranged by size: 

                 918     803     778     370     359     160     128      81
                  45
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2992    3642 
   Size:       2992     650
 
BclI T'GATC_A

Cuts at:      0    2063    3642 
   Size:       2063    1579
 
BglI GCCn_nnn'nGGC

Cuts at:      0     259     863    1717    2129    3637    3642 
   Size:        259     604     854     412    1508       5

  Fragments arranged by size: 

                1508     854     604     412     259       5
 
BglII A'GATC_T

Cuts at:      0    1289    2708    3642 
   Size:       1289    1419     934

  Fragments arranged by size: 

                1419    1289     934
 
BlpI GC'TnA_GC

Cuts at:      0    1257    3613    3642 
   Size:       1257    2356      29

  Fragments arranged by size: 

                2356    1257      29
 
Bme1580I G_kGCm'C

Cuts at:      0     748     859    1981    2808    3642 
   Size:        748     111    1122     827     834

  Fragments arranged by size: 

                1122     834     827     748     111
 
BmgBI CAC'GTC

Cuts at:      0     134     563    3642 
   Size:        134     429    3079

  Fragments arranged by size: 

                3079     429     134
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     453    1255    2492    2685    3642 
   Size:        453     802    1237     193     957

  Fragments arranged by size: 

                1237     957     802     453     193
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     117    1840    3642 
   Size:        117    1723    1802

  Fragments arranged by size: 

                1802    1723     117
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2470    2488    2635    3481    3556    3642 
   Size:       2470      18     147     846      75      86

  Fragments arranged by size: 

                2470     846     147      86      75      18
 
Bpu10I CC'TnA_GC

Cuts at:      0    3247    3642 
   Size:       3247     395
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1633    3535    3642 
   Size:       1633    1902     107

  Fragments arranged by size: 

                1902    1633     107
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1757    3642 
   Size:       1757    1885
 
BsaBI GATnn'nnATC

Cuts at:      0    2713    3642 
   Size:       2713     929
 
BsaHI Gr'CG_yC

Cuts at:      0      17    3642 
   Size:         17    3625
 
BsaWI w'CCGG_w

Cuts at:      0    1115    1759    3642 
   Size:       1115     644    1883

  Fragments arranged by size: 

                1883    1115     644
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2902    2932    3407    3437    3642 
   Size:       2902      30     475      30     205

  Fragments arranged by size: 

                2902     475     205      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      79    1368    1371    1494    1603    1729    1969    2037 
   Size:         79    1289       3     123     109     126     240      68

Cuts at:   2037    2938    2941    3457    3642 
   Size:        901       3     516     185

  Fragments arranged by size: 

                1289     901     516     240     185     126     123     109
                  79      68       3       3
 
BseYI C'CCAG_C

Cuts at:      0    1151    1200    1250    1277    2111    2530    3213    3642 
   Size:       1151      49      50      27     834     419     683     429

  Fragments arranged by size: 

                1151     834     683     429     419      50      49      27
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     451    2391    2413    2761    2887    3514    3642 
   Size:        451    1940      22     348     126     627     128

  Fragments arranged by size: 

                1940     627     451     348     128     126      22
 
BsiEI CG_ry'CG

Cuts at:      0     662    1577    1798    3642 
   Size:        662     915     221    1844

  Fragments arranged by size: 

                1844     915     662     221
 
BsiHKAI G_wGCw'C

Cuts at:      0     446     748    1520    1585    1618    2360    2842    3141 
   Size:        446     302     772      65      33     742     482     299

Cuts at:   3141    3225    3467    3527    3642 
   Size:         84     242      60     115

  Fragments arranged by size: 

                 772     742     482     446     302     299     242     115
                  84      65      60      33
 
BsmI GAATG_Cn'

Cuts at:      0    1908    3642 
   Size:       1908    1734
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1519    2658    3145    3642 
   Size:       1519    1139     487     497

  Fragments arranged by size: 

                1519    1139     497     487
 
Bsp1286I G_dGCh'C

Cuts at:      0      86      98     446     455     748     859    1258    1520 
   Size:         86      12     348       9     293     111     399     262

Cuts at:   1520    1585    1618    1981    2360    2808    2842    3098    3141 
   Size:         65      33     363     379     448      34     256      43

Cuts at:   3141    3225    3293    3420    3467    3520    3527    3642 
   Size:         84      68     127      47      53       7     115

  Fragments arranged by size: 

                 448     399     379     363     348     293     262     256
                 127     115     111      86      84      68      65      53
                  47      43      34      33      12       9       7
 
BspEI T'CCGG_A

Cuts at:      0    1115    3642 
   Size:       1115    2527
 
BspHI T'CATG_A

Cuts at:      0    1061    2288    3642 
   Size:       1061    1227    1354

  Fragments arranged by size: 

                1354    1227    1061
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2733    3642 
   Size:       2733     909
 
BsrBI CCG'CTC

Cuts at:      0    1215    3540    3642 
   Size:       1215    2325     102

  Fragments arranged by size: 

                2325    1215     102
 
BsrDI GCAATG_nn'

Cuts at:      0     537     704    1706    3642 
   Size:        537     167    1002    1936

  Fragments arranged by size: 

                1936    1002     537     167
 
BssSI C'ACGA_G

Cuts at:      0    3355    3642 
   Size:       3355     287
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     536     752    1748    3642 
   Size:        536     216     996    1894

  Fragments arranged by size: 

                1894     996     536     216
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1326    2940    3057    3642 
   Size:       1326    1614     117     585

  Fragments arranged by size: 

                1614    1326     585     117
 
BstYI r'GATC_y

Cuts at:      0     143     288    1289    2153    2708    3192    3318    3590 
   Size:        143     145    1001     864     555     484     126     272

Cuts at:   3590    3642 
   Size:         52

  Fragments arranged by size: 

                1001     864     555     484     272     145     143     126
                  52
 
BtgI C'CryG_G

Cuts at:      0     815     893    3056    3268    3642 
   Size:        815      78    2163     212     374

  Fragments arranged by size: 

                2163     815     374     212      78
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1280    2366    3642 
   Size:       1280    1086    1276

  Fragments arranged by size: 

                1280    1276    1086
 
BtsI GCAGTG_nn'

Cuts at:      0     777    3153    3345    3642 
   Size:        777    2376     192     297

  Fragments arranged by size: 

                2376     777     297     192
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     747     782    3642 
   Size:        747      35    2860

  Fragments arranged by size: 

                2860     747      35
 
DraI TTT'AAA

Cuts at:      0    3369    3642 
   Size:       3369     273
 
DraIII CAC_nnn'GTG

Cuts at:      0     752     830    1494    1974    3269    3642 
   Size:        752      78     664     480    1295     373

  Fragments arranged by size: 

                1295     752     664     480     373      78
 
EaeI y'GGCC_r

Cuts at:      0    1508    1604    1795    2648    3642 
   Size:       1508      96     191     853     994

  Fragments arranged by size: 

                1508     994     853     191      96
 
EagI C'GGCC_G

Cuts at:      0    1795    3642 
   Size:       1795    1847
 
EarI CTCTTCn'nnn_

Cuts at:      0    1438    3296    3580    3642 
   Size:       1438    1858     284      62

  Fragments arranged by size: 

                1858    1438     284      62
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      14      38     106    2096    3185    3340    3642 
   Size:         14      24      68    1990    1089     155     302

  Fragments arranged by size: 

                1990    1089     302     155      68      24      14
 
EcoICRI GAG'CTC

Cuts at:      0    1583    3139    3525    3642 
   Size:       1583    1556     386     117

  Fragments arranged by size: 

                1583    1556     386     117
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1386    1742    1867    2470    2488    2635    2866    3117 
   Size:       1386     356     125     603      18     147     231     251

Cuts at:   3117    3481    3556    3642 
   Size:        364      75      86

  Fragments arranged by size: 

                1386     603     364     356     251     231     147     125
                  86      75      18
 
EcoO109I rG'GnC_Cy

Cuts at:      0      31    1155    2010    2967    3546    3555    3642 
   Size:         31    1124     855     957     579       9      87

  Fragments arranged by size: 

                1124     957     855     579      87      31       9
 
EcoRV GAT'ATC

Cuts at:      0    2327    3642 
   Size:       2327    1315
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     390    3510    3642 
   Size:        390    3120     132

  Fragments arranged by size: 

                3120     390     132
 
FspI TGC'GCA

Cuts at:      0    3145    3642 
   Size:       3145     497
 
HaeII r_GCGC'y

Cuts at:      0      20     612     740     798    3626    3642 
   Size:         20     592     128      58    2828      16

  Fragments arranged by size: 

                2828     592     128      58      20      16
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      55      87     463     495     535     567    1474    1506 
   Size:         55      32     376      32      40      32     907      32

Cuts at:   1506    1651    1683    2902    2934    3303    3335    3642 
   Size:        145      32    1219      32     369      32     307

  Fragments arranged by size: 

                1219     907     376     369     307     145      55      40
                  32      32      32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0     333    3642 
   Size:        333    3309
 
HindIII A'AGCT_T

Cuts at:      0     226    3642 
   Size:        226    3416
 
KasI G'GCGC_C

Cuts at:      0      16    3642 
   Size:         16    3626
 
KpnI G_GTAC'C

Cuts at:      0    2567    3642 
   Size:       2567    1075
 
MfeI C'AATT_G

Cuts at:      0     492    3642 
   Size:        492    3150
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      98     171     695    1283    3642 
   Size:         98      73     524     588    2359

  Fragments arranged by size: 

                2359     588     524      98      73
 
MscI TGG'CCA

Cuts at:      0    1606    3642 
   Size:       1606    2036
 
MslI CAynn'nnrTG

Cuts at:      0    1321    2644    2938    3642 
   Size:       1321    1323     294     704

  Fragments arranged by size: 

                1323    1321     704     294
 
MspA1I CmG'CkG

Cuts at:      0     938    1200    3189    3213    3642 
   Size:        938     262    1989      24     429

  Fragments arranged by size: 

                1989     938     429     262      24
 
NarI GG'CG_CC

Cuts at:      0      17    3642 
   Size:         17    3625
 
NcoI C'CATG_G

Cuts at:      0     815    3056    3642 
   Size:        815    2241     586

  Fragments arranged by size: 

                2241     815     586
 
NspI r_CATG'y

Cuts at:      0    2643    3642 
   Size:       2643     999
 
PacI TTA_AT'TAA

Cuts at:      0    2228    3642 
   Size:       2228    1414
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     714    1293    2555    3642 
   Size:        714     579    1262    1087

  Fragments arranged by size: 

                1262    1087     714     579
 
PfoI T'CCnGG_A

Cuts at:      0    3113    3642 
   Size:       3113     529
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    3407    3439    3642 
   Size:       3407      32     203

  Fragments arranged by size: 

                3407     203      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      31    1155    2010    2967    3546    3555    3642 
   Size:         31    1124     855     957     579       9      87

  Fragments arranged by size: 

                1124     957     855     579      87      31       9
 
PstI C_TGCA'G

Cuts at:      0     788    3106    3188    3642 
   Size:        788    2318      82     454

  Fragments arranged by size: 

                2318     788     454      82
 
PvuI CG_AT'CG

Cuts at:      0     662    3642 
   Size:        662    2980
 
PvuII CAG'CTG

Cuts at:      0     938    1200    3189    3213    3642 
   Size:        938     262    1989      24     429

  Fragments arranged by size: 

                1989     938     429     262      24
 
SacI G_AGCT'C

Cuts at:      0    1585    3141    3527    3642 
   Size:       1585    1556     386     115

  Fragments arranged by size: 

                1585    1556     386     115
 
SanDI GG'GwC_CC

Cuts at:      0      31    2010    3642 
   Size:         31    1979    1632

  Fragments arranged by size: 

                1979    1632      31
 
SbfI CC_TGCA'GG

Cuts at:      0     788    3642 
   Size:        788    2854
 
ScaI AGT'ACT

Cuts at:      0    1244    3642 
   Size:       1244    2398
 
SfcI C'TryA_G

Cuts at:      0     784    2671    3102    3184    3642 
   Size:        784    1887     431      82     458

  Fragments arranged by size: 

                1887     784     458     431      82
 
SfoI GGC'GCC

Cuts at:      0      18    3642 
   Size:         18    3624
 
SmlI C'TyrA_G

Cuts at:      0    1648    1814    2657    3550    3642 
   Size:       1648     166     843     893      92

  Fragments arranged by size: 

                1648     893     843     166      92
 
SphI G_CATG'C

Cuts at:      0    2643    3642 
   Size:       2643     999
 
StuI AGG'CCT

Cuts at:      0    1375    1561    1675    2758    3642 
   Size:       1375     186     114    1083     884

  Fragments arranged by size: 

                1375    1083     884     186     114
 
StyI C'CwwG_G

Cuts at:      0     815    1655    2015    2085    2567    2718    3056    3569 
   Size:        815     840     360      70     482     151     338     513

Cuts at:   3569    3642 
   Size:         73

  Fragments arranged by size: 

                 840     815     513     482     360     338     151      73
                  70
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     472     906    1291    1591    2375    2950    3254    3642 
   Size:        472     434     385     300     784     575     304     388

  Fragments arranged by size: 

                 784     575     472     434     388     385     304     300
            
 
TatI w'GTAC_w

Cuts at:      0     461    1242    3642 
   Size:        461     781    2400

  Fragments arranged by size: 

                2400     781     461
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     559     591    3582    3614    3642 
   Size:        559      32    2991      32      28

  Fragments arranged by size: 

                2991     559      32      32      28
 
XhoI C'TCGA_G

Cuts at:      0    1814    2657    3642 
   Size:       1814     843     985

  Fragments arranged by size: 

                1814     985     843
 
XmnI GAAnn'nnTTC

Cuts at:      0    1537    3130    3642 
   Size:       1537    1593     512

  Fragments arranged by size: 

                1593    1537     512
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI     AfeI     AhdI     AlfI     AloI    AlwNI
    ApaLI     ApoI     AseI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BssSI   BstAPI    BstXI    BstYI     BtgI    BtgZI     BtsI
    CspCI     DraI   DraIII     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI EcoO109I    EcoRV     FalI     FspI    HaeII    Hin4I   HincII
  HindIII     KasI     KpnI     MfeI     MmeI     MscI     MslI   MspA1I
     NarI     NcoI     NspI     PacI    PflMI     PfoI     PpiI    PpuMI
     PstI     PvuI    PvuII     SacI    SanDI     SbfI     ScaI     SfcI
     SfoI     SmlI     SphI     StuI     StyI    TaqII     TatI     TstI
     XhoI     XmnI

 Enzymes that do not cut: 

    AatII     AccI     AclI    AflII   AflIII     AgeI     AleI     ApaI
     AscI    AsiSI    BbvCI     BcgI    BfrBI     BmtI    BsaAI    BsiWI
    BsrFI    BsrGI   BssHII    BstBI   BstEII  BstZ17I   Bsu36I     ClaI
     DrdI    EcoNI    EcoRI     FseI    FspAI     HpaI     MluI     NaeI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PasI     PciI
     PmeI     PmlI    PshAI     PsiI   PspOMI    PspXI     PsrI    RsrII
    SacII     SalI     SapI    SexAI     SfiI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SrfI     SspI     SwaI  Tth111I     XbaI     XcmI
     XmaI     ZraI
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