Restriction map for
pF1KE2322
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# NO CUT ENZYMES ( 80 enzymes) #
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AatII Acc65I AclI AflII AflIII
AgeI AhdI AocI AscI AseI
AsnI Asp718I AspEI AspI AvrII
BamHI BbrPI BcgI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsiWI Bsp1407I BspLU11I BsrGI
BssHII Bst1107I BstZ17I Bsu36I CelII
CpoI DraI Eam1105I Eco81I EcoRI
EcoRV EcoT22I FbaI HindIII HpaI
KpnI KspI MamI MfeI MluI
MunI NdeI NheI NotI NruI
NsiI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacII SalI SexAI
SfiI SgrAI SmlI SnaBI SpeI
SspBI SwaI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 33 enzymes) #
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Aor51HI 1795 AGCGCT AGC^GCT
Asp700I 2781 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 3904 GGCGCC GGCGC^C
BbvCI 2274 CCTCAGC CCTCAGC(-5/-2)
BfaI 1031 CTAG C^TAG
BsmBI 3425 GAGACG CGTCTC(1/5)
BspDI 262 ATCGAT AT^CGAT
BsrDI 1674 CATTGC GCAATG(2/0)
BstBI 1040 TTCGAA TT^CGAA
BstEII 3324 GGTGACC G^GT[ACGT]ACC
BstPI 3324 GGTGACC G^GT[ACGT]ACC
ClaI 262 ATCGAT AT^CGAT
DrdI 3507 GACGAGTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1795 AGCGCT AGC^GCT
EcoO65I 3324 GGTGACC G^GT[ACGT]ACC
FseI 2710 GGCCGGCC GGCCGG^CC
KasI 3904 GGCGCC G^GCGCC
MaeI 1031 CTAG C^TAG
NaeI 2711 GCCGGC GCC^GGC
NarI 3904 GGCGCC GG^CGCC
NgoMIV 2711 GCCGGC G^CCGGC
NspV 1040 TTCGAA TT^CGAA
SbfI 1308 CCTGCAGG CCTGCA^GG
SfoI 3904 GGCGCC GGC^GCC
SfuI 1040 TTCGAA TT^CGAA
SmaI 986 CCCGGG CCC^GGG
SphI 836 GCATGC GCATG^C
Sse8387I 1308 CCTGCAGG CCTGCA^GG
SspI 3048 AATATT AAT^ATT
StuI 3647 AGGCCT AGG^CCT
XmaCI 986 CCCGGG C^CCGGG
XmaI 986 CCCGGG C^CCGGG
XmnI 2781 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AccI 577 GTCTAC GT^[AC][GT]AC
3574 GTCTAC
AccIII 1976 TCCGGA T^CCGGA
3440 TCCGGA
ApaI 347 GGGCCC GGGCC^C
2796 GGGCCC
AviII 1849 TGCGCA TGC^GCA
3707 TGCGCA
BsaAI 1116 CACGTA [CT]AC^GT[GA]
1423 TACGTG
BseAI 1976 TCCGGA T^CCGGA
3440 TCCGGA
BsiEI 2536 CGGCCG CG[GA][CT]^CG
2713 CGGCCG
BspEI 1976 TCCGGA T^CCGGA
3440 TCCGGA
BspMI 542 ACCTGC ACCTGC(4/8)
1311 GCAGGT
BsrBI 3531 CCGCTC CCGCTC(-3/-3)
2959 GAGCGG
BstAPI 1707 GCACCCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2027 GCAATTTCTGC
EagI 2536 CGGCCG C^GGCCG
2713 CGGCCG
EclXI 2536 CGGCCG C^GGCCG
2713 CGGCCG
Eco52I 2536 CGGCCG C^GGCCG
2713 CGGCCG
EcoNI 36 CCTGGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1524 CCTCGTGAAGG
FspI 1849 TGCGCA TGC^GCA
3707 TGCGCA
MroI 1976 TCCGGA T^CCGGA
3440 TCCGGA
MseI 1321 TTAA T^TAA
2411 TTAA
PshAI 1159 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1372 GACCTGGGTC
SapI 2926 GAAGAGC GCTCTTC(1/4)
1005 GCTCTTC
TfiI 2239 GAATC G^A[AT]TC
3523 GATTC
Tru9I 1321 TTAA T^TAA
2411 TTAA
XcmI 2294 CCAGCAGCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3908 CCATCAGCTGCCTGG
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# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
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AccII AciI AcsI AcyI AfaI
AluI Alw44I AlwI AlwNI ApaLI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BglI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspHI BsrFI
BsrI BssKI BssSI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RcaI
RsaI SacI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI StyI TaqI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII