Restriction map for
pF1KE2322
(Linear) (Six-base) MAPSORT of: KIEE2322.seq Check: 3420 from: 1 to: 4005
KIEE2322.orf
With 182 enzymes: SgfI *
June 19, 2019 14:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1302 4005
Size: 1302 2703
AccI GT'mk_AC
Cuts at: 0 578 3575 4005
Size: 578 2997 430
Fragments arranged by size:
2997 578 430
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 993 1450 1645 3733 4005
Size: 993 457 195 2088 272
Fragments arranged by size:
2088 993 457 272 195
AfeI AGC'GCT
Cuts at: 0 1797 4005
Size: 1797 2208
AleI CACnn'nnGTG
Cuts at: 0 1876 4005
Size: 1876 2129
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2471 4005
Size: 2471 1534
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2956 2988 4005
Size: 2956 32 1017
Fragments arranged by size:
2956 1017 32
AlwNI CAG_nnn'CTG
Cuts at: 0 648 1317 1751 2223 3137 3284 4005
Size: 648 669 434 472 914 147 721
Fragments arranged by size:
914 721 669 648 472 434 147
ApaI G_GGCC'C
Cuts at: 0 351 2800 4005
Size: 351 2449 1205
Fragments arranged by size:
2449 1205 351
ApaLI G'TGCA_C
Cuts at: 0 242 1330 2136 2605 4005
Size: 242 1088 806 469 1400
Fragments arranged by size:
1400 1088 806 469 242
ApoI r'AATT_y
Cuts at: 0 312 1044 3088 3291 4005
Size: 312 732 2044 203 714
Fragments arranged by size:
2044 732 714 312 203
AvaI C'yCGr_G
Cuts at: 0 986 1451 1837 2314 4005
Size: 986 465 386 477 1691
Fragments arranged by size:
1691 986 477 465 386
BanI G'GyrC_C
Cuts at: 0 462 858 2719 3271 3904 4005
Size: 462 396 1861 552 633 101
Fragments arranged by size:
1861 633 552 462 396 101
BanII G_rGCy'C
Cuts at: 0 351 1447 1969 2164 2629 2800 3344 3421
Size: 351 1096 522 195 465 171 544 77
Cuts at: 3421 3857 4005
Size: 436 148
Fragments arranged by size:
1096 544 522 465 436 351 195 171
148 77
BbeI G_GCGC'C
Cuts at: 0 3908 4005
Size: 3908 97
BbsI GAAGACnn'nnnn_
Cuts at: 0 172 235 489 1851 4005
Size: 172 63 254 1362 2154
Fragments arranged by size:
2154 1362 254 172 63
BbvCI CC'TCA_GC
Cuts at: 0 2275 4005
Size: 2275 1730
BglI GCCn_nnn'nGGC
Cuts at: 0 739 2518 2717 4005
Size: 739 1779 199 1288
Fragments arranged by size:
1779 1288 739 199
Bme1580I G_kGCm'C
Cuts at: 0 246 351 467 1334 2140 2609 2722 2800
Size: 246 105 116 867 806 469 113 78
Cuts at: 2800 4005
Size: 1205
Fragments arranged by size:
1205 867 806 469 246 116 113 105
78
BmgBI CAC'GTC
Cuts at: 0 404 551 2484 3429 4005
Size: 404 147 1933 945 576
Fragments arranged by size:
1933 945 576 404 147
BmrI ACTGGGnnnn_n'
Cuts at: 0 2113 3159 3399 4005
Size: 2113 1046 240 606
Fragments arranged by size:
2113 1046 606 240
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 165 670 1423 1708 2072 2640 2904 2959
Size: 165 505 753 285 364 568 264 55
Cuts at: 2959 4005
Size: 1046
Fragments arranged by size:
1046 753 568 505 364 285 264 165
55
Bpu10I CC'TnA_GC
Cuts at: 0 132 2275 3420 4005
Size: 132 2143 1145 585
Fragments arranged by size:
2143 1145 585 132
BsaI GGTCTCn'nnnn_
Cuts at: 0 2338 2481 3394 4005
Size: 2338 143 913 611
Fragments arranged by size:
2338 913 611 143
BsaAI yAC'GTr
Cuts at: 0 1118 1425 4005
Size: 1118 307 2580
Fragments arranged by size:
2580 1118 307
BsaHI Gr'CG_yC
Cuts at: 0 866 919 2638 3071 3905 4005
Size: 866 53 1719 433 834 100
Fragments arranged by size:
1719 866 834 433 100 53
BsaWI w'CCGG_w
Cuts at: 0 1592 1976 3440 4005
Size: 1592 384 1464 565
Fragments arranged by size:
1592 1464 565 384
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 571 601 2545 2575 2950 2956 2980 2986
Size: 571 30 1944 30 375 6 24 6
Cuts at: 2986 3958 3988 4005
Size: 972 30 17
Fragments arranged by size:
1944 972 571 375 30 30 30 24
17 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 442 1810 2338 2370 2550 3383 3400 4005
Size: 442 1368 528 32 180 833 17 605
Fragments arranged by size:
1368 833 605 528 442 180 32 17
BseYI C'CCAG_C
Cuts at: 0 1946 2734 2903 3005 3373 4005
Size: 1946 788 169 102 368 632
Fragments arranged by size:
1946 788 632 368 169 102
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 384 1068 2019 2431 2884 3493 4005
Size: 384 684 951 412 453 609 512
Fragments arranged by size:
951 684 609 512 453 412 384
BsiEI CG_ry'CG
Cuts at: 0 2539 2716 4005
Size: 2539 177 1289
Fragments arranged by size:
2539 1289 177
BsiHKAI G_wGCw'C
Cuts at: 0 246 983 1334 1447 2140 2164 2609 2933
Size: 246 737 351 113 693 24 445 324
Cuts at: 2933 3421 3629 3857 4005
Size: 488 208 228 148
Fragments arranged by size:
737 693 488 445 351 324 246 228
208 148 113 24
BsmI GAATG_Cn'
Cuts at: 0 259 1243 3607 4005
Size: 259 984 2364 398
Fragments arranged by size:
2364 984 398 259
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3419 4005
Size: 3419 586
Bsp1286I G_dGCh'C
Cuts at: 0 246 351 467 983 1334 1447 1969 2140
Size: 246 105 116 516 351 113 522 171
Cuts at: 2140 2164 2609 2629 2722 2800 2933 3344 3421
Size: 24 445 20 93 78 133 411 77
Cuts at: 3421 3629 3857 4005
Size: 208 228 148
Fragments arranged by size:
522 516 445 411 351 246 228 208
171 148 133 116 113 105 93 78
77 24 20
BspEI T'CCGG_A
Cuts at: 0 1976 3440 4005
Size: 1976 1464 565
Fragments arranged by size:
1976 1464 565
BspHI T'CATG_A
Cuts at: 0 89 1800 2912 3413 3611 4005
Size: 89 1711 1112 501 198 394
Fragments arranged by size:
1711 1112 501 394 198 89
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 551 1302 4005
Size: 551 751 2703
Fragments arranged by size:
2703 751 551
BsrBI CCG'CTC
Cuts at: 0 2961 3533 4005
Size: 2961 572 472
Fragments arranged by size:
2961 572 472
BsrDI GCAATG_nn'
Cuts at: 0 1673 4005
Size: 1673 2332
BsrFI r'CCGG_y
Cuts at: 0 1334 2685 2711 3403 4005
Size: 1334 1351 26 692 602
Fragments arranged by size:
1351 1334 692 602 26
BssSI C'ACGA_G
Cuts at: 0 1509 1525 3201 4005
Size: 1509 16 1676 804
Fragments arranged by size:
1676 1509 804 16
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1713 2033 4005
Size: 1713 320 1972
Fragments arranged by size:
1972 1713 320
BstBI TT'CG_AA
Cuts at: 0 1041 4005
Size: 1041 2964
BstEII G'GTnAC_C
Cuts at: 0 3324 4005
Size: 3324 681
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1269 1686 1941 2673 4005
Size: 1269 417 255 732 1332
Fragments arranged by size:
1332 1269 732 417 255
BstYI r'GATC_y
Cuts at: 0 890 941 990 1490 1748 2918 2948 3443
Size: 890 51 49 500 258 1170 30 495
Cuts at: 3443 4005
Size: 562
Fragments arranged by size:
1170 890 562 500 495 258 51 49
30
BtgI C'CryG_G
Cuts at: 0 456 1262 1970 2039 2858 3215 3950 4005
Size: 456 806 708 69 819 357 735 55
Fragments arranged by size:
819 806 735 708 456 357 69 55
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 951 2493 3096 4005
Size: 951 1542 603 909
Fragments arranged by size:
1542 951 909 603
BtsI GCAGTG_nn'
Cuts at: 0 1199 1394 1505 3209 4005
Size: 1199 195 111 1704 796
Fragments arranged by size:
1704 1199 796 195 111
ClaI AT'CG_AT
Cuts at: 0 263 4005
Size: 263 3742
DraIII CAC_nnn'GTG
Cuts at: 0 462 1929 3107 3681 4005
Size: 462 1467 1178 574 324
Fragments arranged by size:
1467 1178 574 462 324
DrdI GACnn_nn'nnGTC
Cuts at: 0 3513 4005
Size: 3513 492
EaeI y'GGCC_r
Cuts at: 0 453 897 1462 1539 1730 1931 2486 2536
Size: 453 444 565 77 191 201 555 50
Cuts at: 2536 2675 2709 2713 3231 3257 3672 3947 4005
Size: 139 34 4 518 26 415 275 58
Fragments arranged by size:
565 555 518 453 444 415 275 201
191 139 77 58 50 34 26 4
EagI C'GGCC_G
Cuts at: 0 2536 2713 4005
Size: 2536 177 1292
Fragments arranged by size:
2536 1292 177
EarI CTCTTCn'nnn_
Cuts at: 0 32 1000 1012 2054 2921 4005
Size: 32 968 12 1042 867 1084
Fragments arranged by size:
1084 1042 968 867 32 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1072 1534 1582 2844 3808 4005
Size: 1072 462 48 1262 964 197
Fragments arranged by size:
1262 1072 964 462 197 48
EcoICRI GAG'CTC
Cuts at: 0 1445 2162 3419 3855 4005
Size: 1445 717 1257 436 150
Fragments arranged by size:
1445 1257 717 436 150
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 165 670 993 1423 1450 1645 1708 2072
Size: 165 505 323 430 27 195 63 364
Cuts at: 2072 2640 2904 2959 3733 4005
Size: 568 264 55 774 272
Fragments arranged by size:
774 568 505 430 364 323 272 264
195 165 63 55 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 40 1528 4005
Size: 40 1488 2477
Fragments arranged by size:
2477 1488 40
EcoO109I rG'GnC_Cy
Cuts at: 0 280 347 1146 1378 2728 2796 4005
Size: 280 67 799 232 1350 68 1209
Fragments arranged by size:
1350 1209 799 280 232 68 67
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1675 4005
Size: 1675 2330
FseI GG_CCGG'CC
Cuts at: 0 2715 4005
Size: 2715 1290
FspI TGC'GCA
Cuts at: 0 1851 3709 4005
Size: 1851 1858 296
Fragments arranged by size:
1858 1851 296
FspAI rTGC'GCAy
Cuts at: 0 3709 4005
Size: 3709 296
HaeII r_GCGC'y
Cuts at: 0 1799 2294 2891 3908 4005
Size: 1799 495 597 1017 97
Fragments arranged by size:
1799 1017 597 495 97
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 141 173 252 256 267 284 288 299
Size: 141 32 79 4 11 17 4 11
Cuts at: 299 2545 2577 3842 3874 4005
Size: 2246 32 1265 32 131
Fragments arranged by size:
2246 1265 141 131 79 32 32 32
17 11 11 4 4
HincII GTy'rAC
Cuts at: 0 480 1158 1629 4005
Size: 480 678 471 2376
Fragments arranged by size:
2376 678 480 471
KasI G'GCGC_C
Cuts at: 0 3904 4005
Size: 3904 101
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1850 4005
Size: 1850 2155
MscI TGG'CCA
Cuts at: 0 455 1732 1933 2488 2677 3233 4005
Size: 455 1277 201 555 189 556 772
Fragments arranged by size:
1277 772 556 555 455 201 189
MslI CAynn'nnrTG
Cuts at: 0 449 1120 1267 1876 3214 3976 4005
Size: 449 671 147 609 1338 762 29
Fragments arranged by size:
1338 762 671 609 449 147 29
MspA1I CmG'CkG
Cuts at: 0 636 726 1176 1236 1296 1585 1847 1855
Size: 636 90 450 60 60 289 262 8
Cuts at: 1855 1950 2734 3500 3848 3897 3914 4005
Size: 95 784 766 348 49 17 91
Fragments arranged by size:
784 766 636 450 348 289 262 95
91 90 60 60 49 17 8
NaeI GCC'GGC
Cuts at: 0 2713 4005
Size: 2713 1292
NarI GG'CG_CC
Cuts at: 0 3905 4005
Size: 3905 100
NcoI C'CATG_G
Cuts at: 0 1262 1970 3215 4005
Size: 1262 708 1245 790
Fragments arranged by size:
1262 1245 790 708
NgoMIV G'CCGG_C
Cuts at: 0 2711 4005
Size: 2711 1294
NspI r_CATG'y
Cuts at: 0 840 1125 1581 3354 3809 4005
Size: 840 285 456 1773 455 196
Fragments arranged by size:
1773 840 456 455 285 196
PasI CC'CwG_GG
Cuts at: 0 924 1382 2304 4005
Size: 924 458 922 1701
Fragments arranged by size:
1701 924 922 458
PflMI CCAn_nnn'nTGG
Cuts at: 0 971 1728 1929 4005
Size: 971 757 201 2076
Fragments arranged by size:
2076 971 757 201
PfoI T'CCnGG_A
Cuts at: 0 2549 4005
Size: 2549 1456
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1750 1782 2956 2988 4005
Size: 1750 32 1174 32 1017
Fragments arranged by size:
1750 1174 1017 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 280 1146 1378 4005
Size: 280 866 232 2627
Fragments arranged by size:
2627 866 280 232
PshAI GACnn'nnGTC
Cuts at: 0 1163 1376 4005
Size: 1163 213 2629
Fragments arranged by size:
2629 1163 213
PspOMI G'GGCC_C
Cuts at: 0 347 2796 4005
Size: 347 2449 1209
Fragments arranged by size:
2449 1209 347
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 3181 3213 4005
Size: 3181 32 792
Fragments arranged by size:
3181 792 32
PstI C_TGCA'G
Cuts at: 0 548 1313 1893 2051 3659 4005
Size: 548 765 580 158 1608 346
Fragments arranged by size:
1608 765 580 548 346 158
PvuII CAG'CTG
Cuts at: 0 726 1176 1236 1296 1585 1950 3500 3914
Size: 726 450 60 60 289 365 1550 414
Cuts at: 3914 4005
Size: 91
Fragments arranged by size:
1550 726 450 414 365 289 91 60
60
SacI G_AGCT'C
Cuts at: 0 1447 2164 3421 3857 4005
Size: 1447 717 1257 436 148
Fragments arranged by size:
1447 1257 717 436 148
SanDI GG'GwC_CC
Cuts at: 0 1378 4005
Size: 1378 2627
SapI GCTCTTCn'nnn_
Cuts at: 0 1012 2921 4005
Size: 1012 1909 1084
Fragments arranged by size:
1909 1084 1012
SbfI CC_TGCA'GG
Cuts at: 0 1313 4005
Size: 1313 2692
ScaI AGT'ACT
Cuts at: 0 1549 2498 3874 4005
Size: 1549 949 1376 131
Fragments arranged by size:
1549 1376 949 131
SfcI C'TryA_G
Cuts at: 0 544 631 1309 1812 1889 2047 3116 3655
Size: 544 87 678 503 77 158 1069 539
Cuts at: 3655 4005
Size: 350
Fragments arranged by size:
1069 678 544 539 503 350 158 87
77
SfoI GGC'GCC
Cuts at: 0 3906 4005
Size: 3906 99
SmaI CCC'GGG
Cuts at: 0 988 4005
Size: 988 3017
SphI G_CATG'C
Cuts at: 0 840 4005
Size: 840 3165
SspI AAT'ATT
Cuts at: 0 3050 4005
Size: 3050 955
StuI AGG'CCT
Cuts at: 0 3649 4005
Size: 3649 356
StyI C'CwwG_G
Cuts at: 0 343 422 1262 1866 1970 3215 4005
Size: 343 79 840 604 104 1245 790
Fragments arranged by size:
1245 840 790 604 343 104 79
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 916 1548 1716 3627 4005
Size: 916 632 168 1911 378
Fragments arranged by size:
1911 916 632 378 168
TatI w'GTAC_w
Cuts at: 0 518 1547 2496 2744 3872 3980 4005
Size: 518 1029 949 248 1128 108 25
Fragments arranged by size:
1128 1029 949 518 248 108 25
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1495 1527 2950 2982 4005
Size: 1495 32 1423 32 1023
Fragments arranged by size:
1495 1423 1023 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2301 3915 4005
Size: 2301 1614 90
Fragments arranged by size:
2301 1614 90
XmaI C'CCGG_G
Cuts at: 0 986 4005
Size: 986 3019
XmnI GAAnn'nnTTC
Cuts at: 0 2785 4005
Size: 2785 1220
Enzymes that do cut:
AarI AccI AcuI AfeI AleI AlfI AloI AlwNI
ApaI ApaLI ApoI AvaI BanI BanII BbeI BbsI
BbvCI BglI Bme1580I BmgBI BmrI BpmI Bpu10I BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FseI
FspI FspAI HaeII Hin4I HincII KasI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PflMI PfoI PpiI PpuMI PshAI PspOMI PsrI PstI
PvuII SacI SanDI SapI SbfI ScaI SfcI SfoI
SmaI SphI SspI StuI StyI TaqII TatI TstI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AscI
AseI AsiSI AvrII BaeI BamHI BcgI BciVI BclI
BfrBI BglII BlpI BmtI BplI BpuEI BsaBI BsiWI
BsrGI BssHII BstZ17I Bsu36I CspCI DraI EcoRI EcoRV
HindIII HpaI KpnI MfeI MluI NdeI NheI NotI
NruI NsiI PacI PciI PmeI PmlI PsiI PspXI
PvuI RsrII SacII SalI SexAI SfiI SgfI SgrAI
SmlI SnaBI SpeI SrfI SwaI Tth111I XbaI XhoI
ZraI