Restriction map for pF1KE2305
##################################
# NO CUT ENZYMES (   43 enzymes) #
##################################

AclI        AcsI        AflII       ApoI        AscI        
AseI        AsnI        Asp700I     BfrI        BsaBI       
Bsp1407I    BspDI       BsrGI       Bst1107I    BstBI       
BstZ17I     ClaI        CpoI        DraI        EcoRI       
EcoRV       HindIII     HpaI        MamI        MfeI        
MunI        NdeI        NspV        PacI        PmeI        
PshBI       Psp1406I    PvuI        RsrII       SbfI        
SfuI        SnaBI       SpeI        Sse8387I    SspBI       
SspI        SwaI        XmnI        


###################################
# ONE CUT ENZYMES (   28 enzymes) #
###################################

AatII        3925  GACGTC               GACGT^C
Acc65I       5360  GGTACC               G^GTACC
AgeI         9038  ACCGGT               A^CCGGT
Asp718I      5360  GGTACC               G^GTACC
AvrII        4845  CCTAGG               C^CTAGG
BclI         2517  TGATCA               T^GATCA
BlnI         4845  CCTAGG               C^CTAGG
BsiWI        1156  CGTACG               C^GTACG
BspLU11I     3842  ACATGT               A^CATGT
BstEII       2772  GGTGACC              G^GT[ACGT]ACC
BstPI        2772  GGTGACC              G^GT[ACGT]ACC
DrdI         3595  GACCCCGATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I      2772  GGTGACC              G^GT[ACGT]ACC
EcoT22I      9309  ATGCAT               ATGCA^T
FbaI         2517  TGATCA               T^GATCA
KpnI         5360  GGTACC               GGTAC^C
MluI         1035  ACGCGT               A^CGCGT
MseI         2246  TTAA                 T^TAA
NheI         3996  GCTAGC               G^CTAGC
NotI           12  GCGGCCGC             GC^GGCCGC
NsiI         9309  ATGCAT               ATGCA^T
PinAI        9038  ACCGGT               A^CCGGT
SalI           42  GTCGAC               G^TCGAC
ScaI         2060  AGTACT               AGT^ACT
SgrAI        1059  CGCCGGCG             C[GA]^CCGG[CT]G
StuI         5694  AGGCCT               AGG^CCT
Tru9I        2246  TTAA                 T^TAA
XbaI         9202  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AspI         8557  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             9685  GACTCGGTC           
AviII        1853  TGCGCA               TGC^GCA
             7691  TGCGCA              
BamHI        7071  GGATCC               G^GATCC
             7718  GGATCC              
BglII        2945  AGATCT               A^GATCT
             6400  AGATCT              
BsmBI        8481  CGTCTC               CGTCTC(1/5)
             9875  CGTCTC              
DraIII       3757  CACAGCGTG            CAC[ACGT][ACGT][ACGT]^GTG
             5806  CACCGAGTG           
FseI         1876  GGCCGGCC             GGCCGG^CC
             4425  GGCCGGCC            
FspI         1853  TGCGCA               TGC^GCA
             7691  TGCGCA              
NruI         1326  TCGCGA               TCG^CGA
             4628  TCGCGA              
PflFI        8557  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             9685  GACTCGGTC           
PshAI         383  GACCAGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             3144  GACTCCAGTC          
SfiI         5581  GGCCGGCGGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             8281  GGCCTCCAGGGCC       
SphI          824  GCATGC               GCATG^C
             7886  GCATGC              
Tth111I      8557  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             9685  GACTCGGTC           


#########################################
# 3 OR MORE CUT ENZYMES (  173 enzymes) #
#########################################

AccI        AccII       AccIII      AciI        AcyI        
AfaI        AflIII      AhdI        AluI        Alw44I      
AlwI        AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AspEI       AspHI       AvaI        AvaII       
BalI        BanI        BanII       BbeI        BbrPI       
BbsI        BbvCI       BbvI        BcgI        BciVI       
BcnI        BfaI        BglI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaHI       
BsaI        BsaJI       BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsmI        BsoBI       Bsp1286I    
BspEI       BspHI       BspMI       BsrBI       BsrDI       
BsrFI       BsrI        BssHII      BssKI       BssSI       
BstAPI      BstF5I      BstNI       BstUI       BstXI       
BstYI       Bsu36I      Cac8I       CelII       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HinfI       HpaII       HphI        ItaI        KasI        
Ksp632I     KspI        MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MroI        MscI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NaeI        NarI        
NciI        NcoI        NdeII       NgoMIV      NlaIII      
NlaIV       NspI        PaeR7I      PflMI       PleI        
PmaCI       PmlI        PpuMI       PstI        PvuII       
RcaI        RsaI        SacI        SacII       SapI        
Sau3AI      Sau96I      ScrFI       SexAI       SfaNI       
SfcI        SfoI        SmaI        SmlI        StyI        
TaqI        TfiI        TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XcmI        XhoI        
XhoII       XmaCI       XmaI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com