Restriction map for pF1KE2305
 (Linear) (Six-base) MAPSORT of: KIEE2305.seq  Check: 7338  from: 1  to: 9936



KIEE2305.orf

 With 182 enzymes: SgfI * 

                        December 13, 2010 18:21  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1647    4474    5920    6002    6716    9936 
   Size:       1647    2827    1446      82     714    3220

  Fragments arranged by size: 

                3220    2827    1647    1446     714      82
 
AatII G_ACGT'C

Cuts at:      0    3929    9936 
   Size:       3929    6007
 
AccI GT'mk_AC

Cuts at:      0      43    1082    2342    9936 
   Size:         43    1039    1260    7594

  Fragments arranged by size: 

                7594    1260    1039      43
 
Acc65I G'GTAC_C

Cuts at:      0    5360    9936 
   Size:       5360    4576
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     500     926    5214    5689    7145    8714    9052    9936 
   Size:        500     426    4288     475    1456    1569     338     884

  Fragments arranged by size: 

                4288    1569    1456     884     500     475     426     338
            
 
AfeI AGC'GCT

Cuts at:      0    1645    3856    5218    9936 
   Size:       1645    2211    1362    4718

  Fragments arranged by size: 

                4718    2211    1645    1362
 
AflIII A'CryG_T

Cuts at:      0    1035    3842    4130    4370    9936 
   Size:       1035    2807     288     240    5566

  Fragments arranged by size: 

                5566    2807    1035     288     240
 
AgeI A'CCGG_T

Cuts at:      0    9038    9936 
   Size:       9038     898
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1224    2148    8844    9579    9936 
   Size:       1224     924    6696     735     357

  Fragments arranged by size: 

                6696    1224     924     735     357
 
AleI CACnn'nnGTG

Cuts at:      0    1027    1252    1687    2320    2629    3271    5174    6053 
   Size:       1027     225     435     633     309     642    1903     879

Cuts at:   6053    7084    7630    9936 
   Size:       1031     546    2306

  Fragments arranged by size: 

                2306    1903    1031    1027     879     642     633     546
                 435     309     225
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1045    1382    3661    4946    7512    9376    9936 
   Size:       1045     337    2279    1285    2566    1864     560

  Fragments arranged by size: 

                2566    2279    1864    1285    1045     560     337
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     326     358    9936 
   Size:        326      32    9578

  Fragments arranged by size: 

                9578     326      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     391     766     996    2956    3654    5667    5733    6362 
   Size:        391     375     230    1960     698    2013      66     629

Cuts at:   6362    7207    7872    8292    8377    8398    8587    9841    9936 
   Size:        845     665     420      85      21     189    1254      95

  Fragments arranged by size: 

                2013    1960    1254     845     698     665     629     420
                 391     375     230     189      95      85      66      21
            
 
ApaI G_GGCC'C

Cuts at:      0     302    4034    4531    4864    5008    5861    6468    6852 
   Size:        302    3732     497     333     144     853     607     384

Cuts at:   6852    7020    9009    9808    9854    9936 
   Size:        168    1989     799      46      82

  Fragments arranged by size: 

                3732    1989     853     799     607     497     384     333
                 302     168     144      82      46
 
ApaLI G'TGCA_C

Cuts at:      0    1738    2530    3463    4853    6266    7507    7803    9936 
   Size:       1738     792     933    1390    1413    1241     296    2133

  Fragments arranged by size: 

                2133    1738    1413    1390    1241     933     792     296
            
 
AvaI C'yCGr_G

Cuts at:      0      31     486     492     714     727     805     833     871 
   Size:         31     455       6     222      13      78      28      38

Cuts at:    871     885    1564    2183    2798    3886    5038    5487    6458 
   Size:         14     679     619     615    1088    1152     449     971

Cuts at:   6458    7010    8333    9068    9380    9446    9485    9599    9936 
   Size:        552    1323     735     312      66      39     114     337

  Fragments arranged by size: 

                1323    1152    1088     971     735     679     619     615
                 552     455     449     337     312     222     114      78
                  66      39      38      31      28      14      13       6
            
 
AvrII C'CTAG_G

Cuts at:      0    4845    9936 
   Size:       4845    5091
 
BamHI G'GATC_C

Cuts at:      0    7071    7718    9936 
   Size:       7071     647    2218

  Fragments arranged by size: 

                7071    2218     647
 
BanI G'GyrC_C

Cuts at:      0     113     404     533     598     813     999    1021    1073 
   Size:        113     291     129      65     215     186      22      52

Cuts at:   1073    1815    2079    2314    3967    4462    4516    4667    5140 
   Size:        742     264     235    1653     495      54     151     473

Cuts at:   5140    5360    5990    6047    6887    7288    7918    8470    8915 
   Size:        220     630      57     840     401     630     552     445

Cuts at:   8915    9057    9073    9092    9394    9441    9618    9629    9936 
   Size:        142      16      19     302      47     177      11     307

  Fragments arranged by size: 

                1653     840     742     630     630     552     495     473
                 445     401     307     302     291     264     235     220
                 215     186     177     151     142     129     113      65
                  57      54      52      47      22      19      16      11
            
 
BanII G_rGCy'C

Cuts at:      0     263     302     471     493    3041    3250    4034    4148 
   Size:        263      39     169      22    2548     209     784     114

Cuts at:   4148    4307    4531    4864    5008    5488    5861    6006    6103 
   Size:        159     224     333     144     480     373     145      97

Cuts at:   6103    6250    6306    6468    6518    6557    6852    6862    7020 
   Size:        147      56     162      50      39     295      10     158

Cuts at:   7020    7760    8042    8225    8908    9009    9341    9592    9808 
   Size:        740     282     183     683     101     332     251     216

Cuts at:   9808    9854    9936 
   Size:         46      82

  Fragments arranged by size: 

                2548     784     740     683     480     373     333     332
                 295     282     263     251     224     216     209     183
                 169     162     159     158     147     145     144     114
                 101      97      82      56      50      46      39      39
                  22      10
 
BbeI G_GCGC'C

Cuts at:      0     408     817    1077    4520    4671    7922    8474    8919 
   Size:        408     409     260    3443     151    3251     552     445

Cuts at:   8919    9398    9936 
   Size:        479     538

  Fragments arranged by size: 

                3443    3251     552     538     479     445     409     408
                 260     151
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     191     694    3189    3921    8158    9936 
   Size:        191     503    2495     732    4237    1778

  Fragments arranged by size: 

                4237    2495    1778     732     503     191
 
BbvCI CC'TCA_GC

Cuts at:      0      85    2214    3206    3920    7840    9936 
   Size:         85    2129     992     714    3920    2096

  Fragments arranged by size: 

                3920    2129    2096     992     714      85
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    4528    4562    7422    7456    7794    7828    9361    9395 
   Size:       4528      34    2860      34     338      34    1533      34

Cuts at:   9395    9936 
   Size:        541

  Fragments arranged by size: 

                4528    2860    1533     541     338      34      34      34
                  34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    3166    3549    5064    6594    9936 
   Size:       3166     383    1515    1530    3342

  Fragments arranged by size: 

                3342    3166    1530    1515     383
 
BclI T'GATC_A

Cuts at:      0    2517    9936 
   Size:       2517    7419
 
BfrBI ATG'CAT

Cuts at:      0    9311    9936 
   Size:       9311     625
 
BglI GCCn_nnn'nGGC

Cuts at:      0      21    1066    1796    4511    5249    5588    6086    7732 
   Size:         21    1045     730    2715     738     339     498    1646

Cuts at:   7732    8288    8537    9369    9564    9627    9936 
   Size:        556     249     832     195      63     309

  Fragments arranged by size: 

                2715    1646    1045     832     738     730     556     498
                 339     309     249     195      63      21
 
BglII A'GATC_T

Cuts at:      0    2945    6400    9936 
   Size:       2945    3455    3536

  Fragments arranged by size: 

                3536    3455    2945
 
BlpI GC'TnA_GC

Cuts at:      0     135    4888    6626    6976    7684    8580    9936 
   Size:        135    4753    1738     350     708     896    1356

  Fragments arranged by size: 

                4753    1738    1356     896     708     350     135
 
Bme1580I G_kGCm'C

Cuts at:      0     116     302     601     939    1742    2317    2511    2534 
   Size:        116     186     299     338     803     575     194      23

Cuts at:   2534    2583    3134    3467    3970    4034    4465    4507    4531 
   Size:         49     551     333     503      64     431      42      24

Cuts at:   4531    4857    4864    5008    5861    6050    6082    6270    6289 
   Size:        326       7     144     853     189      32     188      19

Cuts at:   6289    6452    6468    6852    7020    7511    7807    8502    9009 
   Size:        163      16     384     168     491     296     695     507

Cuts at:   9009    9078    9166    9444    9808    9854    9936 
   Size:         69      88     278     364      46      82

  Fragments arranged by size: 

                 853     803     695     575     551     507     503     491
                 431     384     364     338     333     326     299     296
                 278     194     189     188     186     168     163     144
                 116      88      82      69      64      49      46      42
                  32      24      23      19      16       7
 
BmgBI CAC'GTC

Cuts at:      0    2658    2859    3468    8640    9875    9936 
   Size:       2658     201     609    5172    1235      61

  Fragments arranged by size: 

                5172    2658    1235     609     201      61
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2700    4559    6995    7095    7260    9936 
   Size:       2700    1859    2436     100     165    2676

  Fragments arranged by size: 

                2700    2676    2436    1859     165     100
 
BmtI G_CTAG'C

Cuts at:      0    4000    9936 
   Size:       4000    5936
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     435     779    3060    9936 
   Size:        435     344    2281    6876

  Fragments arranged by size: 

                6876    2281     435     344
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1138    2710    3131    3383    5578    6532    6913    7396 
   Size:       1138    1572     421     252    2195     954     381     483

Cuts at:   7396    7615    7627    8070    8269    8593    9355    9936 
   Size:        219      12     443     199     324     762     581

  Fragments arranged by size: 

                2195    1572    1138     954     762     581     483     443
                 421     381     324     252     219     199      12
 
Bpu10I CC'TnA_GC

Cuts at:      0      85     180     305    1046    2214    3206    3335    3920 
   Size:         85      95     125     741    1168     992     129     585

Cuts at:   3920    7840    9080    9286    9936 
   Size:       3920    1240     206     650

  Fragments arranged by size: 

                3920    1240    1168     992     741     650     585     206
                 129     125      95      85
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    3856    4803    7152    7684    9525    9936 
   Size:       3856     947    2349     532    1841     411

  Fragments arranged by size: 

                3856    2349    1841     947     532     411
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     420     844    1312    2127    2180    2913    4291    5613 
   Size:        420     424     468     815      53     733    1378    1322

Cuts at:   5613    9936 
   Size:       4323

  Fragments arranged by size: 

                4323    1378    1322     815     733     468     424     420
                  53
 
BsaAI yAC'GTr

Cuts at:      0    1650    4006    4071    4373    5688    7632    9936 
   Size:       1650    2356      65     302    1315    1944    2304

  Fragments arranged by size: 

                2356    2304    1944    1650    1315     302      65
 
BsaHI Gr'CG_yC

Cuts at:      0     341     405     814    1058    1074    1724    3926    4445 
   Size:        341      64     409     244      16     650    2202     519

Cuts at:   4445    4517    4668    6434    6961    7919    7952    8471    8916 
   Size:         72     151    1766     527     958      33     519     445

Cuts at:   8916    9395    9936 
   Size:        479     541

  Fragments arranged by size: 

                2202    1766     958     650     541     527     519     519
                 479     445     409     341     244     151      72      64
                  33      16
 
BsaWI w'CCGG_w

Cuts at:      0     192     667    3140    4396    9038    9936 
   Size:        192     475    2473    1256    4642     898

  Fragments arranged by size: 

                4642    2473    1256     898     475     192
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      44      74     627     657    3423    3453    4051    4081 
   Size:         44      30     553      30    2766      30     598      30

Cuts at:   4081    6520    6550    9453    9483    9936 
   Size:       2439      30    2903      30     453

  Fragments arranged by size: 

                2903    2766    2439     598     553     453      44      30
                  30      30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      58     109     334     408    1354    1616    3223    3817 
   Size:         58      51     225      74     946     262    1607     594

Cuts at:   3817    4063    5714    5731    6910    7219    7750    8095    8395 
   Size:        246    1651      17    1179     309     531     345     300

Cuts at:   8395    8455    8475    8716    8719    8724    8797    9214    9753 
   Size:         60      20     241       3       5      73     417     539

Cuts at:   9753    9765    9936 
   Size:         12     171

  Fragments arranged by size: 

                1651    1607    1179     946     594     539     531     417
                 345     309     300     262     246     241     225     171
                  74      73      60      58      51      20      17      12
                   5       3
 
BseYI C'CCAG_C

Cuts at:      0      99     122     324     560     571    2117    3201    3585 
   Size:         99      23     202     236      11    1546    1084     384

Cuts at:   3585    3873    5219    5419    5800    6837    7770    7946    7968 
   Size:        288    1346     200     381    1037     933     176      22

Cuts at:   7968    8403    9008    9527    9700    9936 
   Size:        435     605     519     173     236

  Fragments arranged by size: 

                1546    1346    1084    1037     933     605     519     435
                 384     381     288     236     236     202     200     176
                 173      99      23      22      11
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    3190    3445    3739    4452    4657    5074    5434    5732 
   Size:       3190     255     294     713     205     417     360     298

Cuts at:   5732    6782    7462    7810    7858    7885    8144    9119    9305 
   Size:       1050     680     348      48      27     259     975     186

Cuts at:   9305    9936 
   Size:        631

  Fragments arranged by size: 

                3190    1050     975     713     680     631     417     360
                 348     298     294     259     255     205     186      48
                  27
 
BsiEI CG_ry'CG

Cuts at:      0      16      43    1275    1497    2566    4431    6202    8511 
   Size:         16      27    1232     222    1069    1865    1771    2309

Cuts at:   8511    9144    9568    9936 
   Size:        633     424     368

  Fragments arranged by size: 

                2309    1865    1771    1232    1069     633     424     368
                 222      27      16
 
BsiHKAI G_wGCw'C

Cuts at:      0    1514    1742    2534    3467    3506    3908    3977    4181 
   Size:       1514     228     792     933      39     402      69     204

Cuts at:   4181    4307    4857    6062    6270    6374    6557    6998    7511 
   Size:        126     550    1205     208     104     183     441     513

Cuts at:   7511    7760    7807    8892    9463    9592    9936 
   Size:        249      47    1085     571     129     344

  Fragments arranged by size: 

                1514    1205    1085     933     792     571     550     513
                 441     402     344     249     228     208     204     183
                 129     126     104      69      47      39
 
BsiWI C'GTAC_G

Cuts at:      0    1156    9936 
   Size:       1156    8780
 
BsmI GAATG_Cn'

Cuts at:      0    2052    2673    5820    9313    9936 
   Size:       2052     621    3147    3493     623

  Fragments arranged by size: 

                3493    3147    2052     623     621
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    8487    9881    9936 
   Size:       8487    1394      55

  Fragments arranged by size: 

                8487    1394      55
 
Bsp1286I G_dGCh'C

Cuts at:      0     116     263     302     471     493     601     939    1514 
   Size:        116     147      39     169      22     108     338     575

Cuts at:   1514    1742    2317    2511    2534    2583    3041    3134    3250 
   Size:        228     575     194      23      49     458      93     116

Cuts at:   3250    3467    3506    3908    3970    3977    4034    4148    4181 
   Size:        217      39     402      62       7      57     114      33

Cuts at:   4181    4307    4465    4507    4531    4857    4864    5008    5488 
   Size:        126     158      42      24     326       7     144     480

Cuts at:   5488    5861    6006    6050    6062    6082    6103    6250    6270 
   Size:        373     145      44      12      20      21     147      20

Cuts at:   6270    6289    6306    6374    6452    6468    6518    6557    6852 
   Size:         19      17      68      78      16      50      39     295

Cuts at:   6852    6862    6998    7020    7511    7760    7807    8042    8225 
   Size:         10     136      22     491     249      47     235     183

Cuts at:   8225    8502    8892    8908    9009    9078    9166    9341    9444 
   Size:        277     390      16     101      69      88     175     103

Cuts at:   9444    9463    9592    9808    9854    9936 
   Size:         19     129     216      46      82

  Fragments arranged by size: 

                 575     575     491     480     458     402     390     373
                 338     326     295     277     249     235     228     217
                 216     194     183     175     169     158     147     147
                 145     144     136     129     126     116     116     114
                 108     103     101      93      88      82      78      69
                  68      62      57      50      49      47      46      44
                  42      39      39      39      33      24      23      22
                  22      21      20      20      19      19      17      16
                  16      12      10       7       7
 
BspEI T'CCGG_A

Cuts at:      0     192     667    3140    9936 
   Size:        192     475    2473    6796

  Fragments arranged by size: 

                6796    2473     475     192
 
BspHI T'CATG_A

Cuts at:      0    1100    2514    7313    9936 
   Size:       1100    1414    4799    2623

  Fragments arranged by size: 

                4799    2623    1414    1100
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1208    1409    1647    2708    4474    5096    5920    6002 
   Size:       1208     201     238    1061    1766     622     824      82

Cuts at:   6002    6716    7881    9227    9936 
   Size:        714    1165    1346     709

  Fragments arranged by size: 

                1766    1346    1208    1165    1061     824     714     709
                 622     238     201      82
 
BsrBI CCG'CTC

Cuts at:      0    1112    1145    4646    8504    9936 
   Size:       1112      33    3501    3858    1432

  Fragments arranged by size: 

                3858    3501    1432    1112      33
 
BsrDI GCAATG_nn'

Cuts at:      0    3645    4801    8841    9936 
   Size:       3645    1156    4040    1095

  Fragments arranged by size: 

                4040    3645    1156    1095
 
BsrFI r'CCGG_y

Cuts at:      0     549     810    1060    1290    1877    2565    4426    5537 
   Size:        549     261     250     230     587     688    1861    1111

Cuts at:   5537    5582    5987    6790    9038    9290    9626    9936 
   Size:         45     405     803    2248     252     336     310

  Fragments arranged by size: 

                2248    1861    1111     803     688     587     549     405
                 336     310     261     252     250     230      45
 
BssHII G'CGCG_C

Cuts at:      0     604    1432    1434    4349    4549    9936 
   Size:        604     828       2    2915     200    5387

  Fragments arranged by size: 

                5387    2915     828     604     200       2
 
BssSI C'ACGA_G

Cuts at:      0    4705    8035    9638    9936 
   Size:       4705    3330    1603     298

  Fragments arranged by size: 

                4705    3330    1603     298
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2029    3777    4806    5550    6805    7872    8123    9936 
   Size:       2029    1748    1029     744    1255    1067     251    1813

  Fragments arranged by size: 

                2029    1813    1748    1255    1067    1029     744     251
            
 
BstEII G'GTnAC_C

Cuts at:      0    2772    9936 
   Size:       2772    7164
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1251    1254    1734    3673    4755    5302    5646    6089 
   Size:       1251       3     480    1939    1082     547     344     443

Cuts at:   6089    6621    6954    8634    8826    9936 
   Size:        532     333    1680     192    1110

  Fragments arranged by size: 

                1939    1680    1251    1110    1082     547     532     480
                 443     344     333     192       3
 
BstYI r'GATC_y

Cuts at:      0     718    1802    2945    3518    6400    7071    7211    7718 
   Size:        718    1084    1143     573    2882     671     140     507

Cuts at:   7718    8024    9936 
   Size:        306    1912

  Fragments arranged by size: 

                2882    1912    1143    1084     718     671     573     507
                 306     140
 
Bsu36I CC'TnA_GG

Cuts at:      0     477    5726    7245    7363    7994    8378    8551    9936 
   Size:        477    5249    1519     118     631     384     173    1385

  Fragments arranged by size: 

                5249    1519    1385     631     477     384     173     118
            
 
BtgI C'CryG_G

Cuts at:      0      19     537    1025    1221    1767    2037    2190    4736 
   Size:         19     518     488     196     546     270     153    2546

Cuts at:   4736    4754    4913    5144    5301    5356    5639    6009    6082 
   Size:         18     159     231     157      55     283     370      73

Cuts at:   6082    6202    6605    6947    7108    7347    7914    9364    9398 
   Size:        120     403     342     161     239     567    1450      34

Cuts at:   9398    9705    9936 
   Size:        307     231

  Fragments arranged by size: 

                2546    1450     567     546     518     488     403     370
                 342     307     283     270     239     231     231     196
                 161     159     157     153     120      73      55      34
                  19      18
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    4230    9936 
   Size:       4230    5706
 
BtsI GCAGTG_nn'

Cuts at:      0    2520    3122    3266    3278    3778    5715    6082    6177 
   Size:       2520     602     144      12     500    1937     367      95

Cuts at:   6177    6178    6216    6289    6686    6799    7450    7805    8502 
   Size:          1      38      73     397     113     651     355     697

Cuts at:   8502    9815    9936 
   Size:       1313     121

  Fragments arranged by size: 

                2520    1937    1313     697     651     602     500     397
                 367     355     144     121     113      95      73      38
                  12       1
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1749    1784    5181    5216    7695    7730    9936 
   Size:       1749      35    3397      35    2479      35    2206

  Fragments arranged by size: 

                3397    2479    2206    1749      35      35      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    3762    5811    9936 
   Size:       3762    2049    4125

  Fragments arranged by size: 

                4125    3762    2049
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    3601    9936 
   Size:       3601    6335
 
EaeI y'GGCC_r

Cuts at:      0      13    1241    1256    1494    1577    1703    1875    1879 
   Size:         13    1228      15     238      83     126     172       4

Cuts at:   1879    1907    2563    2611    2979    3812    4428    4713    4910 
   Size:         28     656      48     368     833     616     285     197

Cuts at:   4910    5100    5535    5580    6309    6440    6648    7111    8508 
   Size:        190     435      45     729     131     208     463    1397

Cuts at:   8508    8642    8748    8773    8854    9292    9405    9565    9936 
   Size:        134     106      25      81     438     113     160     371

  Fragments arranged by size: 

                1397    1228     833     729     656     616     463     438
                 435     371     368     285     238     208     197     190
                 172     160     134     131     126     113     106      83
                  81      48      45      28      25      15      13       4
            
 
EagI C'GGCC_G

Cuts at:      0      13    1494    2563    4428    8508    9565    9936 
   Size:         13    1481    1069    1865    4080    1057     371

  Fragments arranged by size: 

                4080    1865    1481    1069    1057     371      13
 
EarI CTCTTCn'nnn_

Cuts at:      0     847    3529    3622    4660    5015    5632    6529    7768 
   Size:        847    2682      93    1038     355     617     897    1239

Cuts at:   7768    7864    8314    9204    9936 
   Size:         96     450     890     732

  Fragments arranged by size: 

                2682    1239    1038     897     890     847     732     617
                 450     355      96      93
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     188     444     708    3460    4355    7480    9461    9936 
   Size:        188     256     264    2752     895    3125    1981     475

  Fragments arranged by size: 

                3125    2752    1981     895     475     264     256     188
            
 
EcoICRI GAG'CTC

Cuts at:      0    4305    6555    7758    9590    9936 
   Size:       4305    2250    1203    1832     346

  Fragments arranged by size: 

                4305    2250    1832    1203     346
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     500     926    1138    2710    3131    3383    5214    5578 
   Size:        500     426     212    1572     421     252    1831     364

Cuts at:   5578    5689    6532    6913    7145    7396    7615    7627    8070 
   Size:        111     843     381     232     251     219      12     443

Cuts at:   8070    8269    8593    8714    9052    9355    9936 
   Size:        199     324     121     338     303     581

  Fragments arranged by size: 

                1831    1572     843     581     500     443     426     421
                 381     364     338     324     303     252     251     232
                 219     212     199     121     111      12
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      88     770    3284    5629    9936 
   Size:         88     682    2514    2345    4307

  Fragments arranged by size: 

                4307    2514    2345     682      88
 
EcoO109I rG'GnC_Cy

Cuts at:      0      27     126     223     298     299     436     483     710 
   Size:         27      99      97      75       1     137      47     227

Cuts at:    710    1050    2748    3289    4030    4031    4527    4860    4861 
   Size:        340    1698     541     741       1     496     333       1

Cuts at:   4861    5005    5085    5185    5400    5664    5858    5886    6464 
   Size:        144      80     100     215     264     194      28     578

Cuts at:   6464    6465    6849    7016    7449    7520    7553    7563    7784 
   Size:          1     384     167     433      71      33      10     221

Cuts at:   7784    8289    8407    8414    9299    9489    9546    9805    9851 
   Size:        505     118       7     885     190      57     259      46

Cuts at:   9851    9936 
   Size:         85

  Fragments arranged by size: 

                1698     885     741     578     541     505     496     433
                 384     340     333     264     259     227     221     215
                 194     190     167     144     137     118     100      99
                  97      85      80      75      71      57      47      46
                  33      28      27      10       7       1       1       1
                   1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    4704    5187    9936 
   Size:       4704     483    4749

  Fragments arranged by size: 

                4749    4704     483
 
FseI GG_CCGG'CC

Cuts at:      0    1881    4430    9936 
   Size:       1881    2549    5506

  Fragments arranged by size: 

                5506    2549    1881
 
FspI TGC'GCA

Cuts at:      0    1855    7693    9936 
   Size:       1855    5838    2243

  Fragments arranged by size: 

                5838    2243    1855
 
HaeII r_GCGC'y

Cuts at:      0     284     408     817     870    1077    1647    2111    3858 
   Size:        284     124     409      53     207     570     464    1747

Cuts at:   3858    3997    4025    4087    4520    4671    5220    6838    7922 
   Size:        139      28      62     433     151     549    1618    1084

Cuts at:   7922    8377    8474    8766    8919    9015    9398    9936 
   Size:        455      97     292     153      96     383     538

  Fragments arranged by size: 

                1747    1618    1084     570     549     538     464     455
                 433     409     383     292     284     207     153     151
                 139     124      97      96      62      53      28
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     627     659    1210    1242    1456    1488    2134    2166 
   Size:        627      32     551      32     214      32     646      32

Cuts at:   2166    2179    2211    2430    2462    2710    2742    3073    3105 
   Size:         13      32     219      32     248      32     331      32

Cuts at:   3105    3343    3375    4992    5024    7404    7436    7776    7808 
   Size:        238      32    1617      32    2380      32     340      32

Cuts at:   7808    8662    8694    8830    8862    9565    9597    9936 
   Size:        854      32     136      32     703      32     339

  Fragments arranged by size: 

                2380    1617     854     703     646     627     551     340
                 339     331     248     238     219     214     136      32
                  32      32      32      32      32      32      32      32
                  32      32      32      32      32      32      13
 
HincII GTy'rAC

Cuts at:      0      44    3104    3480    3663    8235    9936 
   Size:         44    3060     376     183    4572    1701

  Fragments arranged by size: 

                4572    3060    1701     376     183      44
 
KasI G'GCGC_C

Cuts at:      0     404     813    1073    4516    4667    7918    8470    8915 
   Size:        404     409     260    3443     151    3251     552     445

Cuts at:   8915    9394    9936 
   Size:        479     542

  Fragments arranged by size: 

                3443    3251     552     542     479     445     409     404
                 260     151
 
KpnI G_GTAC'C

Cuts at:      0    5364    9936 
   Size:       5364    4572
 
MluI A'CGCG_T

Cuts at:      0    1035    9936 
   Size:       1035    8901
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1916    3566    3632    4254    4346    4772    8699    8793 
   Size:       1916    1650      66     622      92     426    3927      94

Cuts at:   8793    9707    9936 
   Size:        914     229

  Fragments arranged by size: 

                3927    1916    1650     914     622     426     229      94
                  92      66
 
MscI TGG'CCA

Cuts at:      0    1909    3814    4715    6442    6650    8644    8856    9936 
   Size:       1909    1905     901    1727     208    1994     212    1080

  Fragments arranged by size: 

                1994    1909    1905    1727    1080     901     212     208
            
 
MslI CAynn'nnrTG

Cuts at:      0     167    1027    1249    1252    1600    1687    1918    2320 
   Size:        167     860     222       3     348      87     231     402

Cuts at:   2320    2525    2629    2975    3271    3733    5096    5174    5300 
   Size:        205     104     346     296     462    1363      78     126

Cuts at:   5300    5344    5644    6053    6770    7082    7084    7514    7630 
   Size:         44     300     409     717     312       2     430     116

Cuts at:   7630    7727    8083    8657    9936 
   Size:         97     356     574    1279

  Fragments arranged by size: 

                1363    1279     860     717     574     462     430     409
                 402     356     348     346     312     300     296     231
                 222     205     167     126     116     104      97      87
                  78      44       3       2
 
MspA1I CmG'CkG

Cuts at:      0     564     848    1027    1362    1429    1444    1493    1887 
   Size:        564     284     179     335      67      15      49     394

Cuts at:   1887    2308    3205    3651    3870    3919    4265    4433    5234 
   Size:        421     897     446     219      49     346     168     801

Cuts at:   5234    6204    6278    6326    6359    6630    6846    7797    7916 
   Size:        970      74      48      33     271     216     951     119

Cuts at:   7916    8535    8587    9130    9213    9531    9570    9609    9666 
   Size:        619      52     543      83     318      39      39      57

Cuts at:   9666    9936 
   Size:        270

  Fragments arranged by size: 

                 970     951     897     801     619     564     543     446
                 421     394     346     335     318     284     271     270
                 219     216     179     168     119      83      74      67
                  57      52      49      49      48      39      39      33
                  15
 
NaeI GCC'GGC

Cuts at:      0     812    1062    1879    4428    5584    5989    6792    9292 
   Size:        812     250     817    2549    1156     405     803    2500

Cuts at:   9292    9628    9936 
   Size:        336     308

  Fragments arranged by size: 

                2549    2500    1156     817     812     803     405     336
                 308     250
 
NarI GG'CG_CC

Cuts at:      0     405     814    1074    4517    4668    7919    8471    8916 
   Size:        405     409     260    3443     151    3251     552     445

Cuts at:   8916    9395    9936 
   Size:        479     541

  Fragments arranged by size: 

                3443    3251     552     541     479     445     409     405
                 260     151
 
NcoI C'CATG_G

Cuts at:      0    2037    2190    5144    5356    5639    6009    6605    6947 
   Size:       2037     153    2954     212     283     370     596     342

Cuts at:   6947    9398    9705    9936 
   Size:       2451     307     231

  Fragments arranged by size: 

                2954    2451    2037     596     370     342     307     283
                 231     212     153
 
NgoMIV G'CCGG_C

Cuts at:      0     810    1060    1877    4426    5582    5987    6790    9290 
   Size:        810     250     817    2549    1156     405     803    2500

Cuts at:   9290    9626    9936 
   Size:        336     310

  Fragments arranged by size: 

                2549    2500    1156     817     810     803     405     336
                 310     250
 
NheI G'CTAG_C

Cuts at:      0    3996    9936 
   Size:       3996    5940
 
NotI GC'GGCC_GC

Cuts at:      0      13    9936 
   Size:         13    9923
 
NruI TCG'CGA

Cuts at:      0    1328    4630    9936 
   Size:       1328    3302    5306

  Fragments arranged by size: 

                5306    3302    1328
 
NsiI A_TGCA'T

Cuts at:      0    9313    9936 
   Size:       9313     623
 
NspI r_CATG'y

Cuts at:      0     828    3846    7890    9936 
   Size:        828    3018    4044    2046

  Fragments arranged by size: 

                4044    3018    2046     828
 
PasI CC'CwG_GG

Cuts at:      0    2418    6468    6501    6853    7743    7941    8862    9936 
   Size:       2418    4050      33     352     890     198     921    1074

  Fragments arranged by size: 

                4050    2418    1074     921     890     352     198      33
            
 
PciI A'CATG_T

Cuts at:      0    3842    9936 
   Size:       3842    6094
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     351     996    1812    1923    2117    3165    3318    6109 
   Size:        351     645     816     111     194    1048     153    2791

Cuts at:   6109    6711    7207    7941    8292    8398    9936 
   Size:        602     496     734     351     106    1538

  Fragments arranged by size: 

                2791    1538    1048     816     734     645     602     496
                 351     351     194     153     111     106
 
PfoI T'CCnGG_A

Cuts at:      0     209    2687    5630    7373    9936 
   Size:        209    2478    2943    1743    2563

  Fragments arranged by size: 

                2943    2563    2478    1743     209
 
PmlI CAC'GTG

Cuts at:      0    4006    4373    5688    7632    9936 
   Size:       4006     367    1315    1944    2304

  Fragments arranged by size: 

                4006    2304    1944    1315     367
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1096    1128    9936 
   Size:       1096      32    8808

  Fragments arranged by size: 

                8808    1096      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     126     436     483    1050    5085    5400    5886    7449 
   Size:        126     310      47     567    4035     315     486    1563

Cuts at:   7449    7520    7553    7563    7784    8407    9299    9489    9546 
   Size:         71      33      10     221     623     892     190      57

Cuts at:   9546    9936 
   Size:        390

  Fragments arranged by size: 

                4035    1563     892     623     567     486     390     315
                 310     221     190     126      71      57      47      33
                  10
 
PshAI GACnn'nnGTC

Cuts at:      0     387    3148    9936 
   Size:        387    2761    6788

  Fragments arranged by size: 

                6788    2761     387
 
PspOMI G'GGCC_C

Cuts at:      0     298    4030    4527    4860    5004    5857    6464    6848 
   Size:        298    3732     497     333     144     853     607     384

Cuts at:   6848    7016    9005    9804    9850    9936 
   Size:        168    1989     799      46      86

  Fragments arranged by size: 

                3732    1989     853     799     607     497     384     333
                 298     168     144      86      46
 
PspXI vC'TCGA_Gb

Cuts at:      0    3886    9936 
   Size:       3886    6050
 
PstI C_TGCA'G

Cuts at:      0    1361    1443    2945    3273    3650    4029    4058    4925 
   Size:       1361      82    1502     328     377     379      29     867

Cuts at:   4925    5663    7543    7674    7812    8897    9936 
   Size:        738    1880     131     138    1085    1039

  Fragments arranged by size: 

                1880    1502    1361    1085    1039     867     738     379
                 377     328     138     131      82      29
 
PvuII CAG'CTG

Cuts at:      0    1362    1429    1444    2308    3205    3651    5234    6326 
   Size:       1362      67      15     864     897     446    1583    1092

Cuts at:   6326    7797    9130    9213    9609    9666    9936 
   Size:       1471    1333      83     396      57     270

  Fragments arranged by size: 

                1583    1471    1362    1333    1092     897     864     446
                 396     270      83      67      57      15
 
SacI G_AGCT'C

Cuts at:      0    4307    6557    7760    9592    9936 
   Size:       4307    2250    1203    1832     344

  Fragments arranged by size: 

                4307    2250    1832    1203     344
 
SacII CC_GC'GG

Cuts at:      0    1028    6205    7917    9936 
   Size:       1028    5177    1712    2019

  Fragments arranged by size: 

                5177    2019    1712    1028
 
SalI G'TCGA_C

Cuts at:      0      42    9936 
   Size:         42    9894
 
SanDI GG'GwC_CC

Cuts at:      0     126     483    7449    7520    9299    9546    9936 
   Size:        126     357    6966      71    1779     247     390

  Fragments arranged by size: 

                6966    1779     390     357     247     126      71
 
SapI GCTCTTCn'nnn_

Cuts at:      0    4660    7864    8314    9936 
   Size:       4660    3204     450    1622

  Fragments arranged by size: 

                4660    3204    1622     450
 
ScaI AGT'ACT

Cuts at:      0    2062    9936 
   Size:       2062    7874
 
SexAI A'CCwGG_T

Cuts at:      0    2768    5516    5574    9614    9936 
   Size:       2768    2748      58    4040     322

  Fragments arranged by size: 

                4040    2768    2748     322      58
 
SfcI C'TryA_G

Cuts at:      0    1357    1439    2941    3269    3646    4025    4054    4921 
   Size:       1357      82    1502     328     377     379      29     867

Cuts at:   4921    5659    7408    7539    7670    7808    8893    9936 
   Size:        738    1749     131     131     138    1085    1043

  Fragments arranged by size: 

                1749    1502    1357    1085    1043     867     738     379
                 377     328     138     131     131      82      29
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    5588    8288    9936 
   Size:       5588    2700    1648

  Fragments arranged by size: 

                5588    2700    1648
 
SfoI GGC'GCC

Cuts at:      0     406     815    1075    4518    4669    7920    8472    8917 
   Size:        406     409     260    3443     151    3251     552     445

Cuts at:   8917    9396    9936 
   Size:        479     540

  Fragments arranged by size: 

                3443    3251     552     540     479     445     409     406
                 260     151
 
SgrAI Cr'CCGG_yG

Cuts at:      0    1060    9936 
   Size:       1060    8876
 
SmaI CCC'GGG

Cuts at:      0     488     835     873     887    1566    6460    7012    9487 
   Size:        488     347      38      14     679    4894     552    2475

Cuts at:   9487    9936 
   Size:        449

  Fragments arranged by size: 

                4894    2475     679     552     488     449     347      38
                  14
 
SmlI C'TyrA_G

Cuts at:      0    2183    3835    3886    4782    7167    7699    9446    9540 
   Size:       2183    1652      51     896    2385     532    1747      94

Cuts at:   9540    9936 
   Size:        396

  Fragments arranged by size: 

                2385    2183    1747    1652     896     532     396      94
                  51
 
SphI G_CATG'C

Cuts at:      0     828    7890    9936 
   Size:        828    7062    2046

  Fragments arranged by size: 

                7062    2046     828
 
StuI AGG'CCT

Cuts at:      0    5696    9936 
   Size:       5696    4240
 
StyI C'CwwG_G

Cuts at:      0     154    1994    2037    2190    4845    4868    5144    5356 
   Size:        154    1840      43     153    2655      23     276     212

Cuts at:   5356    5639    6009    6306    6605    6947    8696    9107    9398 
   Size:        283     370     297     299     342    1749     411     291

Cuts at:   9398    9648    9705    9800    9936 
   Size:        250      57      95     136

  Fragments arranged by size: 

                2655    1840    1749     411     370     342     299     297
                 291     283     276     250     212     154     153     136
                  95      57      43      23
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2431    2650    4315    6022    6460    7096    7642    8827 
   Size:       2431     219    1665    1707     438     636     546    1185

Cuts at:   8827    9678    9936 
   Size:        851     258

  Fragments arranged by size: 

                2431    1707    1665    1185     851     636     546     438
                 258     219
 
TatI w'GTAC_w

Cuts at:      0     980    2060    2990    9936 
   Size:        980    1080     930    6946

  Fragments arranged by size: 

                6946    1080     980     930
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    3182    3214    3423    3455    6061    6093    9453    9485 
   Size:       3182      32     209      32    2606      32    3360      32

Cuts at:   9485    9936 
   Size:        451

  Fragments arranged by size: 

                3360    3182    2606     451     209      32      32      32
                  32
 
Tth111I GACn'n_nGTC

Cuts at:      0    8560    9688    9936 
   Size:       8560    1128     248

  Fragments arranged by size: 

                8560    1128     248
 
XbaI T'CTAG_A

Cuts at:      0    9202    9936 
   Size:       9202     734
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     568    1251    1497    1917    4246    4875    4989    5299 
   Size:        568     683     246     420    2329     629     114     310

Cuts at:   5299    6089    6427    7170    8617    8659    9723    9936 
   Size:        790     338     743    1447      42    1064     213

  Fragments arranged by size: 

                2329    1447    1064     790     743     683     629     568
                 420     338     310     246     213     114      42
 
XhoI C'TCGA_G

Cuts at:      0    2183    3886    9446    9936 
   Size:       2183    1703    5560     490

  Fragments arranged by size: 

                5560    2183    1703     490
 
XmaI C'CCGG_G

Cuts at:      0     486     833     871     885    1564    6458    7010    9485 
   Size:        486     347      38      14     679    4894     552    2475

Cuts at:   9485    9936 
   Size:        451

  Fragments arranged by size: 

                4894    2475     679     552     486     451     347      38
                  14
 
ZraI GAC'GTC

Cuts at:      0    3927    9936 
   Size:       3927    6009
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AcuI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AvaI
    AvrII    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI   BssHII    BssSI   BstAPI   BstEII    BstXI    BstYI
   Bsu36I     BtgI    BtgZI     BtsI    CspCI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI
     FseI     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PasI     PciI    PflMI
     PfoI     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PstI
    PvuII     SacI    SacII     SalI    SanDI     SapI     ScaI    SexAI
     SfcI     SfiI     SfoI    SgrAI     SmaI     SmlI     SphI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     ZraI

 Enzymes that do not cut: 

     AclI    AflII     ApoI     AscI     AseI    AsiSI     BaeI    BsaBI
    BsrGI    BstBI  BstZ17I     ClaI     DraI    EcoRI    EcoRV    FspAI
  HindIII     HpaI     MfeI     NdeI     PacI     PmeI     PsiI     PsrI
     PvuI    RsrII     SbfI     SgfI    SnaBI     SpeI     SrfI     SspI
     SwaI     XmnI
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