Restriction map for pF1KE5724
##################################
# NO CUT ENZYMES (   89 enzymes) #
##################################

Acc65I      AccIII      AclI        AflII       AgeI        
AhdI        Alw44I      ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvrII       BbrPI       BciVI       BfrI        BlnI        
BsaAI       BsaBI       BseAI       BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BsrGI       BssHII      Bst1107I    BstBI       BstEII      
BstPI       BstZ17I     ClaI        CpoI        DraI        
Eam1105I    EcoO65I     EcoRV       EcoT22I     FseI        
HindIII     HpaI        KpnI        MamI        MfeI        
MluI        MroI        MunI        NdeI        NheI        
NotI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SalI        SapI        SbfI        SfiI        
SfuI        SgrAI       SnaBI       SpeI        Sse8387I    
SspBI       SspI        StuI        SwaI        TfiI        
Tth111I     XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AatII         970  GACGTC               GACGT^C
AccI          598  GTCTAC               GT^[AC][GT]AC
AcsI         1650  GAATTC               [GA]^AATT[CT]
AflIII       1109  ACATGT               A^C[GA][CT]GT
Aor51HI      1670  AGCGCT               AGC^GCT
ApoI         1650  GAATTC               [GA]^AATT[CT]
AviII         506  TGCGCA               TGC^GCA
BamHI        1462  GGATCC               G^GATCC
BbeI         1435  GGCGCC               GGCGC^C
BcgI         1181  GCAAGATGTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          293  TGATCA               T^GATCA
BglI         2259  GCCCCCCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI         275  TCCGGT               [AT]^CCGG[AT]
BsgI         2035  CTGCAC               GTGCAG(16/14)
BspLU11I     1109  ACATGT               A^CATGT
BsrDI        1863  CATTGC               GCAATG(2/0)
EagI           16  CGGCCG               C^GGCCG
EclXI          16  CGGCCG               C^GGCCG
Eco47III     1670  AGCGCT               AGC^GCT
Eco52I         16  CGGCCG               C^GGCCG
EcoRI        1650  GAATTC               G^AATTC
FbaI          293  TGATCA               T^GATCA
FspI          506  TGCGCA               TGC^GCA
KasI         1435  GGCGCC               G^GCGCC
MseI         1244  TTAA                 T^TAA
NarI         1435  GGCGCC               GG^CGCC
NruI         1230  TCGCGA               TCG^CGA
PflMI        1050  CCAGTCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII        1838  CAGCTG               CAG^CTG
SacI         1778  GAGCTC               GAGCT^C
SexAI         511  ACCTGGT              A^CC[AT]GGT
SfoI         1435  GGCGCC               GGC^GCC
SmlI         1576  CTTGAG               C^T[CT][GA]AG
SphI          962  GCATGC               GCATG^C
Tru9I        1244  TTAA                 T^TAA
Tsp509I      1651  AATT                 ^AATT
Van91I       1050  CCAGTCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          854  CCATCAACCACATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AocI           98  CCTCAGG              CC^T[ACGT]AGG
             2222  CCTCAGG             
BbsI          382  GTCTTC               GAAGAC(2/6)
             1342  GTCTTC              
BglII         707  AGATCT               A^GATCT
             1478  AGATCT              
BpuAI         382  GTCTTC               GAAGAC(2/6)
             1342  GTCTTC              
BsiEI          16  CGGCCG               CG[GA][CT]^CG
               89  CGACCG              
BsrBI         226  GAGCGG               CCGCTC(-3/-3)
             2209  GAGCGG              
BsrFI          18  GCCGGC               [GA]^CCGG[CT]
             1499  GCCGGC              
BstAPI       1407  GCATGGGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1716  GCACTTGGTGC         
BstXI         734  CCAGTGCTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1798  CCAGTACTGTGG        
Bsu36I         98  CCTCAGG              CC^T[ACGT]AGG
             2222  CCTCAGG             
Cfr10I         18  GCCGGC               [GA]^CCGG[CT]
             1499  GCCGGC              
DraIII        862  CACATGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1717  CACTTGGTG           
DrdI          589  GACGCTGAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              841  GACTGCTGGGTC        
EarI          534  CTCTTC               CTCTTC(1/4)
             1062  CTCTTC              
Eco81I         98  CCTCAGG              CC^T[ACGT]AGG
             2222  CCTCAGG             
HincII        169  GTCAAC               GT[CT]^[GA]AC
              523  GTTGAC              
HindII        169  GTCAAC               GT[CT]^[GA]AC
              523  GTTGAC              
Ksp632I       534  CTCTTC               CTCTTC(1/4)
             1062  CTCTTC              
MaeII         389  ACGT                 A^CGT
              971  ACGT                
NaeI           18  GCCGGC               GCC^GGC
             1499  GCCGGC              
NcoI         1619  CCATGG               C^CATGG
             1990  CCATGG              
NgoMIV         18  GCCGGC               G^CCGGC
             1499  GCCGGC              
ScaI         1157  AGTACT               AGT^ACT
             1800  AGTACT              
TaqI          218  TCGA                 T^CGA
             1190  TCGA                
TthHB8I       218  TCGA                 T^CGA
             1190  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (  106 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       ApaI        AspHI       AvaI        
AvaII       BalI        BanI        BanII       BbvCI       
BbvI        BcnI        BfaI        BlpI        BmyI        
BpmI        Bpu1102I    BsaHI       BsaI        BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrI        
BssKI       BssSI       BstF5I      BstNI       BstUI       
BstYI       Cac8I       CelII       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        KspI        MaeI        
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PleI        PpuMI       
PstI        RsaI        SacII       Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmaI        StyI        
TseI        Tsp45I      TspRI       XhoII       XmaCI       
XmaI        
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