Restriction map for pF1KE5724
 (Linear) (Six-base) MAPSORT of: KIEE5724.seq  Check: 1012  from: 1  to: 2322



KIEE5724.orf

 With 182 enzymes: SgfI * 

                        February 19, 2021 20:23  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1086    2031    2322 
   Size:       1086     945     291

  Fragments arranged by size: 

                1086     945     291
 
AatII G_ACGT'C

Cuts at:      0     974    2322 
   Size:        974    1348
 
AccI GT'mk_AC

Cuts at:      0     599    2322 
   Size:        599    1723
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1816    2322 
   Size:       1816     506
 
AfeI AGC'GCT

Cuts at:      0    1672    2322 
   Size:       1672     650
 
AflIII A'CryG_T

Cuts at:      0    1109    2322 
   Size:       1109    1213
 
AlwNI CAG_nnn'CTG

Cuts at:      0     810    1056    1843    2278    2322 
   Size:        810     246     787     435      44

  Fragments arranged by size: 

                 810     787     435     246      44
 
ApaI G_GGCC'C

Cuts at:      0     618    1767    1888    2322 
   Size:        618    1149     121     434

  Fragments arranged by size: 

                1149     618     434     121
 
ApoI r'AATT_y

Cuts at:      0    1650    2322 
   Size:       1650     672
 
AvaI C'yCGr_G

Cuts at:      0    1439    1745    1767    2051    2120    2294    2322 
   Size:       1439     306      22     284      69     174      28

  Fragments arranged by size: 

                1439     306     284     174      69      28      22
 
BamHI G'GATC_C

Cuts at:      0    1462    2322 
   Size:       1462     860
 
BanI G'GyrC_C

Cuts at:      0      63     140     477     813     951    1003    1435    1971 
   Size:         63      77     337     336     138      52     432     536

Cuts at:   1971    2322 
   Size:        351

  Fragments arranged by size: 

                 536     432     351     337     336     138      77      63
                  52
 
BanII G_rGCy'C

Cuts at:      0     618    1378    1767    1782    1888    2231    2322 
   Size:        618     760     389      15     106     343      91

  Fragments arranged by size: 

                 760     618     389     343     106      91      15
 
BbeI G_GCGC'C

Cuts at:      0    1439    2322 
   Size:       1439     883
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     375    1335    2322 
   Size:        375     960     987

  Fragments arranged by size: 

                 987     960     375
 
BbvCI CC'TCA_GC

Cuts at:      0     593     655    2159    2322 
   Size:        593      62    1504     163

  Fragments arranged by size: 

                1504     593     163      62
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1170    1204    2322 
   Size:       1170      34    1118

  Fragments arranged by size: 

                1170    1118      34
 
BclI T'GATC_A

Cuts at:      0     293    2322 
   Size:        293    2029
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2265    2322 
   Size:       2265      57
 
BglII A'GATC_T

Cuts at:      0     707    1478    2322 
   Size:        707     771     844

  Fragments arranged by size: 

                 844     771     707
 
BlpI GC'TnA_GC

Cuts at:      0     988    1216    1781    1944    2322 
   Size:        988     228     565     163     378

  Fragments arranged by size: 

                 988     565     378     228     163
 
Bme1580I G_kGCm'C

Cuts at:      0     618     818    1767    1888    1976    2322 
   Size:        618     200     949     121      88     346

  Fragments arranged by size: 

                 949     618     346     200     121      88
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     294     798    2322 
   Size:        294     504    1524

  Fragments arranged by size: 

                1524     504     294
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     381    2322 
   Size:        381    1941
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      76     142     595    1895    1940    2075    2287    2322 
   Size:         76      66     453    1300      45     135     212      35

  Fragments arranged by size: 

                1300     453     212     135      76      66      45      35
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     125     593     646     655    2159    2279    2322 
   Size:        125     468      53       9    1504     120      43

  Fragments arranged by size: 

                1504     468     125     120      53      43       9
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1597    2322 
   Size:       1597     725
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     855    2076    2305    2322 
   Size:        855    1221     229      17

  Fragments arranged by size: 

                1221     855     229      17
 
BsaHI Gr'CG_yC

Cuts at:      0     971    1071    1436    2322 
   Size:        971     100     365     886

  Fragments arranged by size: 

                 971     886     365     100
 
BsaWI w'CCGG_w

Cuts at:      0     275    2322 
   Size:        275    2047
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     163     193     568     598    2322 
   Size:        163      30     375      30    1724

  Fragments arranged by size: 

                1724     375     163      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     150     373     469     718    1249    1594    2111    2322 
   Size:        150     223      96     249     531     345     517     211

  Fragments arranged by size: 

                 531     517     345     249     223     211     150      96
            
 
BseYI C'CCAG_C

Cuts at:      0     845    1300    2079    2255    2322 
   Size:        845     455     779     176      67

  Fragments arranged by size: 

                 845     779     455     176      67
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2020    2322 
   Size:       2020     302
 
BsiEI CG_ry'CG

Cuts at:      0      19      92    2322 
   Size:         19      73    2230

  Fragments arranged by size: 

                2230      73      19
 
BsiHKAI G_wGCw'C

Cuts at:      0     428    1169    1774    1782    2322 
   Size:        428     741     605       8     540

  Fragments arranged by size: 

                 741     605     540     428       8
 
Bsp1286I G_dGCh'C

Cuts at:      0     428     618     818    1169    1378    1767    1774    1782 
   Size:        428     190     200     351     209     389       7       8

Cuts at:   1782    1888    1976    2231    2322 
   Size:        106      88     255      91

  Fragments arranged by size: 

                 428     389     351     255     209     200     190     106
                  91      88       8       7
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     512    1086    2031    2322 
   Size:        512     574     945     291

  Fragments arranged by size: 

                 945     574     512     291
 
BsrBI CCG'CTC

Cuts at:      0     228    2211    2322 
   Size:        228    1983     111

  Fragments arranged by size: 

                1983     228     111
 
BsrDI GCAATG_nn'

Cuts at:      0    1862    2322 
   Size:       1862     460
 
BsrFI r'CCGG_y

Cuts at:      0      18    1499    2322 
   Size:         18    1481     823

  Fragments arranged by size: 

                1481     823      18
 
BssSI C'ACGA_G

Cuts at:      0     876    1153    1360    2322 
   Size:        876     277     207     962

  Fragments arranged by size: 

                 962     876     277     207
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1413    1722    2322 
   Size:       1413     309     600

  Fragments arranged by size: 

                1413     600     309
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     741    1805    2322 
   Size:        741    1064     517

  Fragments arranged by size: 

                1064     741     517
 
BstYI r'GATC_y

Cuts at:      0     417     467     641     707    1356    1462    1478    2322 
   Size:        417      50     174      66     649     106      16     844

  Fragments arranged by size: 

                 844     649     417     174     106      66      50      16
            
 
Bsu36I CC'TnA_GG

Cuts at:      0      99    2223    2322 
   Size:         99    2124      99

  Fragments arranged by size: 

                2124      99      99
 
BtgI C'CryG_G

Cuts at:      0      92     481     580    1037    1379    1431    1619    1990 
   Size:         92     389      99     457     342      52     188     371

Cuts at:   1990    2322 
   Size:        332

  Fragments arranged by size: 

                 457     389     371     342     332     188      99      92
                  52
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2189    2322 
   Size:       2189     133
 
BtsI GCAGTG_nn'

Cuts at:      0     215    1310    1701    2322 
   Size:        215    1095     391     621

  Fragments arranged by size: 

                1095     621     391     215
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1905    1940    2322 
   Size:       1905      35     382

  Fragments arranged by size: 

                1905     382      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     867    1722    2322 
   Size:        867     855     600

  Fragments arranged by size: 

                 867     855     600
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     595     847    2322 
   Size:        595     252    1475

  Fragments arranged by size: 

                1475     595     252
 
EaeI y'GGCC_r

Cuts at:      0      16     731    1029    1616    1859    2234    2322 
   Size:         16     715     298     587     243     375      88

  Fragments arranged by size: 

                 715     587     375     298     243      88      16
 
EagI C'GGCC_G

Cuts at:      0      16    2322 
   Size:         16    2306
 
EarI CTCTTCn'nnn_

Cuts at:      0     540    1068    2322 
   Size:        540     528    1254

  Fragments arranged by size: 

                1254     540     528
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     655    2322 
   Size:        655    1667
 
EcoICRI GAG'CTC

Cuts at:      0    1780    2322 
   Size:       1780     542
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      76     142     595    1816    1895    1940    2075    2287 
   Size:         76      66     453    1221      79      45     135     212

Cuts at:   2287    2322 
   Size:         35

  Fragments arranged by size: 

                1221     453     212     135      79      76      66      45
                  35
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     907    2093    2222    2322 
   Size:        907    1186     129     100

  Fragments arranged by size: 

                1186     907     129     100
 
EcoO109I rG'GnC_Cy

Cuts at:      0     245     288     615    1260    1764    1884    1885    2083 
   Size:        245      43     327     645     504     120       1     198

Cuts at:   2083    2116    2322 
   Size:         33     206

  Fragments arranged by size: 

                 645     504     327     245     206     198     120      43
                  33       1
 
EcoRI G'AATT_C

Cuts at:      0    1650    2322 
   Size:       1650     672
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1350    1413    2322 
   Size:       1350      63     909

  Fragments arranged by size: 

                1350     909      63
 
FspI TGC'GCA

Cuts at:      0     508    2322 
   Size:        508    1814
 
HaeII r_GCGC'y

Cuts at:      0    1439    1674    1944    2064    2322 
   Size:       1439     235     270     120     258

  Fragments arranged by size: 

                1439     270     258     235     120
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     517     549    2322 
   Size:        517      32    1773

  Fragments arranged by size: 

                1773     517      32
 
HincII GTy'rAC

Cuts at:      0     171     525    2322 
   Size:        171     354    1797

  Fragments arranged by size: 

                1797     354     171
 
KasI G'GCGC_C

Cuts at:      0    1435    2322 
   Size:       1435     887
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     566    2322 
   Size:        566    1756
 
MscI TGG'CCA

Cuts at:      0     733    1618    1861    2236    2322 
   Size:        733     885     243     375      86

  Fragments arranged by size: 

                 885     733     375     243      86
 
MslI CAynn'nnrTG

Cuts at:      0     448     766     791    1565    2322 
   Size:        448     318      25     774     757

  Fragments arranged by size: 

                 774     757     448     318      25
 
MspA1I CmG'CkG

Cuts at:      0      15      94     241     483    1281    1433    1665    1840 
   Size:         15      79     147     242     798     152     232     175

Cuts at:   1840    1881    1968    2163    2322 
   Size:         41      87     195     159

  Fragments arranged by size: 

                 798     242     232     195     175     159     152     147
                  87      79      41      15
 
NaeI GCC'GGC

Cuts at:      0      20    1501    2322 
   Size:         20    1481     821

  Fragments arranged by size: 

                1481     821      20
 
NarI GG'CG_CC

Cuts at:      0    1436    2322 
   Size:       1436     886
 
NcoI C'CATG_G

Cuts at:      0    1619    1990    2322 
   Size:       1619     371     332

  Fragments arranged by size: 

                1619     371     332
 
NgoMIV G'CCGG_C

Cuts at:      0      18    1499    2322 
   Size:         18    1481     823

  Fragments arranged by size: 

                1481     823      18
 
NruI TCG'CGA

Cuts at:      0    1232    2322 
   Size:       1232    1090
 
NspI r_CATG'y

Cuts at:      0     927     966    1013    1113    1278    1825    2322 
   Size:        927      39      47     100     165     547     497

  Fragments arranged by size: 

                 927     547     497     165     100      47      39
 
PciI A'CATG_T

Cuts at:      0    1109    2322 
   Size:       1109    1213
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1056    2322 
   Size:       1056    1266
 
PfoI T'CCnGG_A

Cuts at:      0    2091    2322 
   Size:       2091     231
 
PpuMI rG'GwC_Cy

Cuts at:      0     288    2083    2116    2322 
   Size:        288    1795      33     206

  Fragments arranged by size: 

                1795     288     206      33
 
PspOMI G'GGCC_C

Cuts at:      0     614    1763    1884    2322 
   Size:        614    1149     121     438

  Fragments arranged by size: 

                1149     614     438     121
 
PstI C_TGCA'G

Cuts at:      0     806     827    1317    1664    2322 
   Size:        806      21     490     347     658

  Fragments arranged by size: 

                 806     658     490     347      21
 
PvuII CAG'CTG

Cuts at:      0    1840    2322 
   Size:       1840     482
 
SacI G_AGCT'C

Cuts at:      0    1782    2322 
   Size:       1782     540
 
SacII CC_GC'GG

Cuts at:      0      95     484    1434    2322 
   Size:         95     389     950     888

  Fragments arranged by size: 

                 950     888     389      95
 
SanDI GG'GwC_CC

Cuts at:      0    2083    2322 
   Size:       2083     239
 
ScaI AGT'ACT

Cuts at:      0    1159    1802    2322 
   Size:       1159     643     520

  Fragments arranged by size: 

                1159     643     520
 
SexAI A'CCwGG_T

Cuts at:      0     511    2322 
   Size:        511    1811
 
SfcI C'TryA_G

Cuts at:      0     264     802     823     956    1313    1660    2322 
   Size:        264     538      21     133     357     347     662

  Fragments arranged by size: 

                 662     538     357     347     264     133      21
 
SfoI GGC'GCC

Cuts at:      0    1437    2322 
   Size:       1437     885
 
SmaI CCC'GGG

Cuts at:      0    1441    2053    2296    2322 
   Size:       1441     612     243      26

  Fragments arranged by size: 

                1441     612     243      26
 
SmlI C'TyrA_G

Cuts at:      0    1576    2322 
   Size:       1576     746
 
SphI G_CATG'C

Cuts at:      0     966    2322 
   Size:        966    1356
 
StyI C'CwwG_G

Cuts at:      0     144     373    1619    1990    2245    2322 
   Size:        144     229    1246     371     255      77

  Fragments arranged by size: 

                1246     371     255     229     144      77
 
TatI w'GTAC_w

Cuts at:      0    1088    1106    1157    1394    1800    2322 
   Size:       1088      18      51     237     406     522

  Fragments arranged by size: 

                1088     522     406     237      51      18
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     861    2322 
   Size:        861    1461
 
XmaI C'CCGG_G

Cuts at:      0    1439    2051    2294    2322 
   Size:       1439     612     243      28

  Fragments arranged by size: 

                1439     612     243      28
 
ZraI GAC'GTC

Cuts at:      0     972    2322 
   Size:        972    1350
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI     AfeI   AflIII    AlwNI     ApaI
     ApoI     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI     BclI     BglI    BglII     BlpI Bme1580I     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspMI    BsrBI    BsrDI
    BsrFI    BssSI   BstAPI    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI    CspCI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII     KasI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NruI     NspI     PciI    PflMI     PfoI
    PpuMI   PspOMI     PstI    PvuII     SacI    SacII    SanDI     ScaI
    SexAI     SfcI     SfoI     SmaI     SmlI     SphI     StyI     TatI
     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI    AflII     AgeI     AhdI     AleI     AlfI     AloI
    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BciVI    BfrBI
    BmgBI     BmtI    BsaAI    BsaBI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BsrGI   BssHII    BstBI   BstEII  BstZ17I     ClaI     DraI
    EcoRV     FseI    FspAI  HindIII     HpaI     KpnI     MfeI     MluI
     NdeI     NheI     NotI     NsiI     PacI     PasI     PmeI     PmlI
     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SalI
     SapI     SbfI     SfiI     SgfI    SgrAI    SnaBI     SpeI     SrfI
     SspI     StuI     SwaI    TaqII     TstI  Tth111I     XbaI     XhoI
     XmnI
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