Restriction map for
pF1KB6207
(Linear) (Six-base) MAPSORT of: KIBB6207.seq Check: 5990 from: 1 to: 1995
KIBB6207.orf
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 523 1107 1443 1498 1666 1695 1863
Size: 448 75 584 336 55 168 29 168
Cuts at: 1863 1995
Size: 132
Fragments arranged by size:
584 448 336 168 168 132 75 55
29
AflIII A'CryG_T
Cuts at: 0 1821 1995
Size: 1821 174
AhdI GACnn_n'nnGTC
Cuts at: 0 1616 1995
Size: 1616 379
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1191 1995
Size: 1191 804
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 959 991 1995
Size: 959 32 1004
Fragments arranged by size:
1004 959 32
ApaI G_GGCC'C
Cuts at: 0 1985 1995
Size: 1985 10
ApaLI G'TGCA_C
Cuts at: 0 1567 1995
Size: 1567 428
ApoI r'AATT_y
Cuts at: 0 989 1032 1995
Size: 989 43 963
Fragments arranged by size:
989 963 43
AvaI C'yCGr_G
Cuts at: 0 371 1066 1995
Size: 371 695 929
Fragments arranged by size:
929 695 371
BanI G'GyrC_C
Cuts at: 0 336 1995
Size: 336 1659
BanII G_rGCy'C
Cuts at: 0 1721 1985 1995
Size: 1721 264 10
Fragments arranged by size:
1721 264 10
BbsI GAAGACnn'nnnn_
Cuts at: 0 1162 1486 1796 1995
Size: 1162 324 310 199
Fragments arranged by size:
1162 324 310 199
BbvCI CC'TCA_GC
Cuts at: 0 908 1995
Size: 908 1087
BciVI GTATCCnnnnn_n'
Cuts at: 0 1571 1995
Size: 1571 424
BclI T'GATC_A
Cuts at: 0 729 1995
Size: 729 1266
BfrBI ATG'CAT
Cuts at: 0 1099 1771 1995
Size: 1099 672 224
Fragments arranged by size:
1099 672 224
BglI GCCn_nnn'nGGC
Cuts at: 0 724 1995
Size: 724 1271
Bme1580I G_kGCm'C
Cuts at: 0 341 1053 1067 1571 1985 1995
Size: 341 712 14 504 414 10
Fragments arranged by size:
712 504 414 341 14 10
BmrI ACTGGGnnnn_n'
Cuts at: 0 140 484 1995
Size: 140 344 1511
Fragments arranged by size:
1511 344 140
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 220 940 1995
Size: 220 720 1055
Fragments arranged by size:
1055 720 220
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 105 273 376 718 935 1019 1439 1691
Size: 105 168 103 342 217 84 420 252
Cuts at: 1691 1995
Size: 304
Fragments arranged by size:
420 342 304 252 217 168 105 103
84
Bpu10I CC'TnA_GC
Cuts at: 0 908 1995
Size: 908 1087
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 964 1647 1995
Size: 964 683 348
Fragments arranged by size:
964 683 348
BsaI GGTCTCn'nnnn_
Cuts at: 0 413 1839 1924 1995
Size: 413 1426 85 71
Fragments arranged by size:
1426 413 85 71
BsaAI yAC'GTr
Cuts at: 0 523 1404 1995
Size: 523 881 591
Fragments arranged by size:
881 591 523
BsaHI Gr'CG_yC
Cuts at: 0 800 1995
Size: 800 1195
BsaWI w'CCGG_w
Cuts at: 0 1249 1544 1995
Size: 1249 295 451
Fragments arranged by size:
1249 451 295
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1582 1612 1995
Size: 1582 30 383
Fragments arranged by size:
1582 383 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 87 687 788 1881 1995
Size: 87 600 101 1093 114
Fragments arranged by size:
1093 600 114 101 87
BseYI C'CCAG_C
Cuts at: 0 158 162 1995
Size: 158 4 1833
Fragments arranged by size:
1833 158 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 126 503 839 1995
Size: 126 377 336 1156
Fragments arranged by size:
1156 377 336 126
BsiHKAI G_wGCw'C
Cuts at: 0 1555 1571 1995
Size: 1555 16 424
Fragments arranged by size:
1555 424 16
BsmI GAATG_Cn'
Cuts at: 0 1441 1525 1786 1995
Size: 1441 84 261 209
Fragments arranged by size:
1441 261 209 84
Bsp1286I G_dGCh'C
Cuts at: 0 341 1053 1067 1555 1571 1721 1985 1995
Size: 341 712 14 488 16 150 264 10
Fragments arranged by size:
712 488 341 264 150 16 14 10
BspEI T'CCGG_A
Cuts at: 0 1544 1995
Size: 1544 451
BsrDI GCAATG_nn'
Cuts at: 0 52 1995
Size: 52 1943
BsrFI r'CCGG_y
Cuts at: 0 380 1995
Size: 380 1615
BsrGI T'GTAC_A
Cuts at: 0 1818 1902 1995
Size: 1818 84 93
Fragments arranged by size:
1818 93 84
BssSI C'ACGA_G
Cuts at: 0 1293 1995
Size: 1293 702
BstAPI GCAn_nnn'nTGC
Cuts at: 0 336 1995
Size: 336 1659
BstXI CCAn_nnnn'nTGG
Cuts at: 0 647 1699 1995
Size: 647 1052 296
Fragments arranged by size:
1052 647 296
BstYI r'GATC_y
Cuts at: 0 267 875 1995
Size: 267 608 1120
Fragments arranged by size:
1120 608 267
Bsu36I CC'TnA_GG
Cuts at: 0 322 1393 1729 1995
Size: 322 1071 336 266
Fragments arranged by size:
1071 336 322 266
BtgI C'CryG_G
Cuts at: 0 29 490 1995
Size: 29 461 1505
Fragments arranged by size:
1505 461 29
BtsI GCAGTG_nn'
Cuts at: 0 787 1995
Size: 787 1208
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 201 236 1512 1547 1995
Size: 201 35 1276 35 448
Fragments arranged by size:
1276 448 201 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 1837 1995
Size: 1837 158
DrdI GACnn_nn'nnGTC
Cuts at: 0 799 1995
Size: 799 1196
EaeI y'GGCC_r
Cuts at: 0 26 382 497 1995
Size: 26 356 115 1498
Fragments arranged by size:
1498 356 115 26
EarI CTCTTCn'nnn_
Cuts at: 0 242 344 1476 1642 1920 1995
Size: 242 102 1132 166 278 75
Fragments arranged by size:
1132 278 242 166 102 75
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 105 273 376 448 523 718 935 1019
Size: 105 168 103 72 75 195 217 84
Cuts at: 1019 1107 1439 1443 1498 1666 1691 1695 1863
Size: 88 332 4 55 168 25 4 168
Cuts at: 1863 1995
Size: 132
Fragments arranged by size:
332 217 195 168 168 168 132 105
103 88 84 75 72 55 25 4
4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1293 1995
Size: 1293 702
EcoO109I rG'GnC_Cy
Cuts at: 0 110 1982 1995
Size: 110 1872 13
Fragments arranged by size:
1872 110 13
EcoRI G'AATT_C
Cuts at: 0 989 1995
Size: 989 1006
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 865 897 1539 1542 1571 1574 1602 1634
Size: 865 32 642 3 29 3 28 32
Cuts at: 1634 1708 1740 1836 1868 1995
Size: 74 32 96 32 127
Fragments arranged by size:
865 642 127 96 74 32 32 32
32 29 28 3 3
HincII GTy'rAC
Cuts at: 0 835 1995
Size: 835 1160
MscI TGG'CCA
Cuts at: 0 499 1995
Size: 499 1496
MslI CAynn'nnrTG
Cuts at: 0 301 889 1266 1518 1995
Size: 301 588 377 252 477
Fragments arranged by size:
588 477 377 301 252
MspA1I CmG'CkG
Cuts at: 0 162 333 1995
Size: 162 171 1662
Fragments arranged by size:
1662 171 162
NcoI C'CATG_G
Cuts at: 0 490 1995
Size: 490 1505
NdeI CA'TA_TG
Cuts at: 0 1515 1995
Size: 1515 480
NsiI A_TGCA'T
Cuts at: 0 1101 1773 1995
Size: 1101 672 222
Fragments arranged by size:
1101 672 222
NspI r_CATG'y
Cuts at: 0 300 1103 1659 1741 1775 1825 1995
Size: 300 803 556 82 34 50 170
Fragments arranged by size:
803 556 300 170 82 50 34
PciI A'CATG_T
Cuts at: 0 1821 1995
Size: 1821 174
PflMI CCAn_nnn'nTGG
Cuts at: 0 1978 1995
Size: 1978 17
PfoI T'CCnGG_A
Cuts at: 0 41 392 1995
Size: 41 351 1603
Fragments arranged by size:
1603 351 41
PmlI CAC'GTG
Cuts at: 0 523 1404 1995
Size: 523 881 591
Fragments arranged by size:
881 591 523
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 703 735 1995
Size: 703 32 1260
Fragments arranged by size:
1260 703 32
PpuMI rG'GwC_Cy
Cuts at: 0 110 1995
Size: 110 1885
PspOMI G'GGCC_C
Cuts at: 0 1981 1995
Size: 1981 14
PstI C_TGCA'G
Cuts at: 0 1170 1995
Size: 1170 825
PvuII CAG'CTG
Cuts at: 0 162 333 1995
Size: 162 171 1662
Fragments arranged by size:
1662 171 162
SfcI C'TryA_G
Cuts at: 0 705 1166 1995
Size: 705 461 829
Fragments arranged by size:
829 705 461
SmlI C'TyrA_G
Cuts at: 0 371 943 1626 1995
Size: 371 572 683 369
Fragments arranged by size:
683 572 371 369
SphI G_CATG'C
Cuts at: 0 1775 1995
Size: 1775 220
SspI AAT'ATT
Cuts at: 0 1213 1995
Size: 1213 782
StyI C'CwwG_G
Cuts at: 0 403 490 1995
Size: 403 87 1505
Fragments arranged by size:
1505 403 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 483 639 1995
Size: 483 156 1356
Fragments arranged by size:
1356 483 156
TatI w'GTAC_w
Cuts at: 0 1818 1902 1995
Size: 1818 84 93
Fragments arranged by size:
1818 93 84
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 192 224 1737 1769 1995
Size: 192 32 1513 32 226
Fragments arranged by size:
1513 226 192 32 32
XhoI C'TCGA_G
Cuts at: 0 371 1995
Size: 371 1624
XmnI GAAnn'nnTTC
Cuts at: 0 1288 1372 1456 1876 1995
Size: 1288 84 84 420 119
Fragments arranged by size:
1288 420 119 84 84
Enzymes that do cut:
AcuI AflIII AhdI AlfI AloI ApaI ApaLI ApoI
AvaI BanI BanII BbsI BbvCI BciVI BclI BfrBI
BglI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspEI BsrDI BsrFI BsrGI BssSI BstAPI
BstXI BstYI Bsu36I BtgI BtsI CspCI DraIII DrdI
EaeI EarI Eco57MI EcoNI EcoO109I EcoRI Hin4I HincII
MscI MslI MspA1I NcoI NdeI NsiI NspI PciI
PflMI PfoI PmlI PpiI PpuMI PspOMI PstI PvuII
SfcI SmlI SphI SspI StyI TaqII TatI TstI
XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AleI AlwNI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BcgI BglII BlpI BmgBI BmtI BsaBI BsiEI
BsiWI BsmBI BspHI BspMI BsrBI BssHII BstBI BstEII
BstZ17I BtgZI ClaI DraI EagI EciI EcoICRI EcoRV
FalI FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MfeI MluI MmeI NaeI NarI NgoMIV NheI
NotI NruI PacI PasI PmeI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI StuI SwaI Tth111I XbaI XcmI
XmaI ZraI