Restriction map for
pF1KB9698
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# NO CUT ENZYMES ( 168 enzymes) #
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AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BcgI
BclI BcnI BfaI BfrI BglI
BglII BlnI BmyI BpuAI BsaAI
BsaBI BsaI BseAI BseRI BsiEI
BsiHKAI BsiWI BsiYI BslI BsmFI
BsmI BsoBI Bsp1286I BspDI BspEI
BspLU11I BsrBI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstYI BstZ17I Bsu36I CfoI
Cfr10I ClaI CpoI DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRV EcoT14I FauI FbaI FseI
FspI HaeII HhaI HinP1I HincII
HindII HpaI KasI KpnI KspI
MaeI MaeII MaeIII MamI MfeI
MflI MluI MluNI MroI MscI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I StuI
StyI SwaI TaqI Tsp45I Tth111I
TthHB8I Van91I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AciI 336 GCGG CCGC(-3/-1)
AcyI 52 GACGCC G[GA]^CG[CT]C
AfaI 609 GTAC GT^AC
AlwNI 308 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 300 GGACC G^G[AT]CC
BciVI 439 GGATAC GTATCC(6/5)
BsaHI 52 GACGCC G[GA]^CG[CT]C
BsaJI 467 CCCTGG C^C[ACGT][ACGT]GG
BsaWI 791 ACCGGA [AT]^CCGG[AT]
BsgI 867 GTGCAG GTGCAG(16/14)
BsmBI 423 GAGACG CGTCTC(1/5)
Bsp1407I 608 TGTACA T^GTACA
BspHI 731 TCATGA T^CATGA
BspMI 211 GCAGGT ACCTGC(4/8)
BsrDI 666 CATTGC GCAATG(2/0)
BsrGI 608 TGTACA T^GTACA
BsrI 106 CCAGT ACTGG(1/-1)
BssKI 468 CCTGG ^CC[ACGT]GG
BstF5I 737 GGATG GGATG(2/0)
BstNI 468 CCTGG CC^[AT]GG
BstXI 572 CCAAATGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 547 GCATGC GC[ACGT]^[ACGT]GC
DpnII 729 GATC ^GATC
EcoRII 468 CCTGG ^CC[AT]GG
FokI 737 GGATG GGATG(9/13)
HaeIII 465 GGCC GG^CC
HapII 792 CCGG C^CGG
HgaI 52 GACGC GACGC(5/10)
Hin1I 52 GACGCC G[GA]^CG[CT]C
HindIII 11 AAGCTT A^AGCTT
HpaII 792 CCGG C^CGG
HphI 813 GGTGA GGTGA(8/7)
MboI 729 GATC ^GATC
MspI 792 CCGG C^CGG
MvaI 468 CCTGG CC^[AT]GG
NdeII 729 GATC ^GATC
NlaIV 300 GGACCC GG[ACGT]^[ACGT]CC
NspI 547 GCATGC [GA]CATG^[CT]
PleI 258 GAGTC GAGTC(4/5)
PpuMI 299 AGGACCC [GA]G^G[AT]CC[CT]
PstI 526 CTGCAG CTGCA^G
RcaI 731 TCATGA T^CATGA
RsaI 609 GTAC GT^AC
SapI 355 GAAGAGC GCTCTTC(1/4)
Sau3AI 729 GATC ^GATC
ScrFI 468 CCTGG CC^[ACGT]GG
SphI 547 GCATGC GCATG^C
SspBI 608 TGTACA T^GTACA
SspI 564 AATATT AAT^ATT
TspRI 87 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 447 CCAGACAAATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 19 enzymes) #
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AccI 101 GTCTAC GT^[AC][GT]AC
135 GTCTAC
BlpI 531 GCTCAGC GC^T[ACGT]AGC
589 GCTGAGC
Bpu1102I 531 GCTCAGC GC^T[ACGT]AGC
589 GCTGAGC
CelII 531 GCTCAGC GC^T[ACGT]AGC
589 GCTGAGC
Cfr13I 300 GGACC G^G[ACGT]CC
465 GGCCC
DraI 415 TTTAAA TTT^AAA
538 TTTAAA
DraII 299 AGGACCC [GA]G^G[ACGT]CC[CT]
464 AGGCCCT
EarI 287 CTCTTC CTCTTC(1/4)
355 GAAGAG
EcoO109I 299 AGGACCC [GA]G^G[ACGT]CC[CT]
464 AGGCCCT
EcoRI 45 GAATTC G^AATTC
490 GAATTC
EcoT22I 79 ATGCAT ATGCA^T
545 ATGCAT
Ksp632I 287 CTCTTC CTCTTC(1/4)
355 GAAGAG
MspA1I 308 CAGCTG C[AC]G^C[GT]G
473 CAGCTG
MwoI 466 GCCCTGGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
673 GCTAAAGATGC
NsiI 79 ATGCAT ATGCA^T
545 ATGCAT
PvuII 308 CAGCTG CAG^CTG
473 CAGCTG
Sau96I 300 GGACC G^G[ACGT]CC
465 GGCCC
SfaNI 679 GATGC GCATC(5/9)
81 GCATC
SfcI 137 CTACAG C^T[GA][CT]AG
526 CTGCAG
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# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AcsI AluI ApoI BbvI BpmI
BsmAI DdeI Fnu4HI HinfI ItaI
MboII MnlI MseI MslI NlaIII
SmlI TfiI Tru9I TseI Tsp509I