Restriction map for pF1KB5447
##################################
# NO CUT ENZYMES (  137 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AcyI        AflII       AflIII      AlwNI       AocI        
Aor51HI     ApaI        ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspI        AvaI        
AviII       AvrII       BbeI        BbrPI       BbvCI       
BcgI        BciVI       BcnI        BfrI        BglI        
BlnI        BlpI        Bpu1102I    BsaBI       BsaHI       
BsaI        BseRI       BsgI        BsiEI       BsiWI       
BsmAI       BsmBI       BsoBI       Bsp1407I    BspDI       
BspLU11I    BsrBI       BsrDI       BsrGI       BssHII      
Bst1107I    BstAPI      BstEII      BstPI       BstXI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraIII      DrdI        DsaI        EagI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT22I     FseI        FspI        
HaeII       HgaI        Hin1I       HindIII     HpaI        
KasI        KpnI        KspI        MamI        MfeI        
MluI        MunI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        PacI        PaeR7I      PflFI       
PflMI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RsrII       SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tth111I     Van91I      XcmI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccII         362  CGCG                 CG^CG
AfaI          369  GTAC                 GT^AC
AgeI          644  ACCGGT               A^CCGGT
AhdI          463  GACATTCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI         463  GACATTCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI          81  GGATCC               G^GATCC
BanI            3  GGCACC               G^G[CT][GA]CC
BclI         1065  TGATCA               T^GATCA
BglII        1003  AGATCT               A^GATCT
BsaAI        1198  TACGTG               [CT]AC^GT[GA]
BsmFI        1045  GGGAC                GGGAC(10/14)
BsmI          537  GAATGC               GAATGC(1/-1)
BspHI         635  TCATGA               T^CATGA
BsrFI         644  ACCGGT               [GA]^CCGG[CT]
BssSI         205  CACGAG               CACGAG(-5/-1)
BstBI         338  TTCGAA               TT^CGAA
BstUI         362  CGCG                 CG^CG
CfoI          361  GCGC                 GCG^C
Cfr10I        644  ACCGGT               [GA]^CCGG[CT]
DraI          457  TTTAAA               TTT^AAA
DraII        1045  GGGACCT              [GA]G^G[ACGT]CC[CT]
Eam1105I      463  GACATTCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          495  GAAGAG               CTCTTC(1/4)
EcoO109I     1045  GGGACCT              [GA]G^G[ACGT]CC[CT]
EcoT14I       264  CCTTGG               C^C[AT][AT]GG
FbaI         1065  TGATCA               T^GATCA
HhaI          361  GCGC                 GCG^C
HinP1I        361  GCGC                 G^CGC
HincII        100  GTCAAC               GT[CT]^[GA]AC
HindII        100  GTCAAC               GT[CT]^[GA]AC
Ksp632I       495  GAAGAG               CTCTTC(1/4)
MaeIII       1225  GTCAC                ^GT[ACGT]AC
MslI          568  CACGCCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        446  CCGCTG               C[AC]G^C[GT]G
MvnI          362  CGCG                 CG^CG
NspI         1076  GCATGT               [GA]CATG^[CT]
NspV          338  TTCGAA               TT^CGAA
PinAI         644  ACCGGT               A^CCGGT
PpuMI        1045  GGGACCT              [GA]G^G[AT]CC[CT]
RcaI          635  TCATGA               T^CATGA
RsaI          369  GTAC                 GT^AC
SacI          943  GAGCTC               GAGCT^C
SfuI          338  TTCGAA               TT^CGAA
SmlI          286  CTCAAG               C^T[CT][GA]AG
StyI          264  CCTTGG               C^C[AT][AT]GG
Tsp45I       1225  GTCAC                ^GT[GC]AC
XbaI         1191  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

Alw44I        332  GTGCAC               G^TGCAC
              575  GTGCAC              
AlwI           82  GATCC                GGATC(4/5)
               81  GGATC               
ApaLI         332  GTGCAC               G^TGCAC
              575  GTGCAC              
BalI          920  TGGCCA               TGG^CCA
             1202  TGGCCA              
BanII         784  GGGCTC               G[GA]GC[CT]^C
              943  GAGCTC              
BbsI          972  GAAGAC               GAAGAC(2/6)
               52  GTCTTC              
BbvI          448  GCTGC                GCAGC(8/12)
             1146  GCTGC               
BfaI         1024  CTAG                 C^TAG
             1192  CTAG                
BpuAI         972  GAAGAC               GAAGAC(2/6)
               52  GTCTTC              
BspMI        1142  ACCTGC               ACCTGC(4/8)
             1107  GCAGGT              
BstYI          81  GGATCC               [GA]^GATC[CT]
             1003  AGATCT              
Cac8I          33  GCAGGC               GC[ACGT]^[ACGT]GC
              922  GCCAGC              
EaeI          920  TGGCCA               [CT]^GGCC[GA]
             1202  TGGCCA              
Fnu4HI        448  GCTGC                GC^[ACGT]GC
             1146  GCTGC               
HphI          412  TCACC                GGTGA(8/7)
             1226  TCACC               
ItaI          448  GCTGC                GC^[ACGT]GC
             1146  GCTGC               
MaeI         1024  CTAG                 C^TAG
             1192  CTAG                
MaeII        1166  ACGT                 A^CGT
             1199  ACGT                
MflI           81  GGATCC               [GA]^GATC[CT]
             1003  AGATCT              
MluNI         920  TGGCCA               TGG^CCA
             1202  TGGCCA              
MscI          920  TGGCCA               TGG^CCA
             1202  TGGCCA              
MseI          458  TTAA                 T^TAA
             1177  TTAA                
PleI          667  GACTC                GAGTC(4/5)
              800  GAGTC               
Tru9I         458  TTAA                 T^TAA
             1177  TTAA                
TseI          448  GCTGC                G^C[AT]GC
             1146  GCTGC               
XhoII          81  GGATCC               [GA]^GATC[CT]
             1003  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   48 enzymes) #
#########################################

AccIII      AciI        AluI        AspHI       AvaII       
BmyI        BpmI        BsaJI       BsaWI       BseAI       
BsiHKAI     BsiYI       BslI        Bsp1286I    BspEI       
BsrI        BssKI       BstF5I      BstNI       Cfr13I      
DdeI        DpnII       EcoRII      FauI        FokI        
HaeIII      HapII       HinfI       HpaII       MboI        
MboII       MnlI        MroI        MspI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       Sau3AI      
Sau96I      ScrFI       SfaNI       TaqI        TfiI        
Tsp509I     TspRI       TthHB8I     
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