Restriction map for pF1KB5447
 (Linear) (Six-base) MAPSORT of: KIBB5447.seq  Check: 6118  from: 1  to: 1239



KIBB5447.orf

 With 182 enzymes: SgfI * 

                        February 15, 2010 17:50  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      57     758    1239 
   Size:         57     701     481

  Fragments arranged by size: 

                 701     481      57
 
AgeI A'CCGG_T

Cuts at:      0     644    1239 
   Size:        644     595
 
AhdI GACnn_n'nnGTC

Cuts at:      0     468    1239 
   Size:        468     771
 
AleI CACnn'nnGTG

Cuts at:      0     572    1239 
   Size:        572     667
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     197    1239 
   Size:        197    1042
 
ApaLI G'TGCA_C

Cuts at:      0     332     575    1239 
   Size:        332     243     664

  Fragments arranged by size: 

                 664     332     243
 
BamHI G'GATC_C

Cuts at:      0      81    1239 
   Size:         81    1158
 
BanI G'GyrC_C

Cuts at:      0       3    1239 
   Size:          3    1236
 
BanII G_rGCy'C

Cuts at:      0     788     947    1239 
   Size:        788     159     292

  Fragments arranged by size: 

                 788     292     159
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      45     979    1239 
   Size:         45     934     260

  Fragments arranged by size: 

                 934     260      45
 
BclI T'GATC_A

Cuts at:      0    1065    1239 
   Size:       1065     174
 
BglII A'GATC_T

Cuts at:      0    1003    1239 
   Size:       1003     236
 
Bme1580I G_kGCm'C

Cuts at:      0     336     579     762    1020    1239 
   Size:        336     243     183     258     219

  Fragments arranged by size: 

                 336     258     243     219     183
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     492     599     756    1100    1239 
   Size:        492     107     157     344     139

  Fragments arranged by size: 

                 492     344     157     139     107
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     496     553     721    1239 
   Size:        496      57     168     518

  Fragments arranged by size: 

                 518     496     168      57
 
Bpu10I CC'TnA_GC

Cuts at:      0     941    1239 
   Size:        941     298
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     271    1239 
   Size:        271     968
 
BsaAI yAC'GTr

Cuts at:      0    1200    1239 
   Size:       1200      39
 
BsaWI w'CCGG_w

Cuts at:      0      84     239     606     644     986    1239 
   Size:         84     155     367      38     342     253

  Fragments arranged by size: 

                 367     342     253     155      84      38
 
BseYI C'CCAG_C

Cuts at:      0      38     279    1239 
   Size:         38     241     960

  Fragments arranged by size: 

                 960     241      38
 
BsiHKAI G_wGCw'C

Cuts at:      0     336     579     912     947    1239 
   Size:        336     243     333      35     292

  Fragments arranged by size: 

                 336     333     292     243      35
 
BsmI GAATG_Cn'

Cuts at:      0     543    1239 
   Size:        543     696
 
Bsp1286I G_dGCh'C

Cuts at:      0     336     579     762     788     912     947    1020    1239 
   Size:        336     243     183      26     124      35      73     219

  Fragments arranged by size: 

                 336     243     219     183     124      73      35      26
            
 
BspEI T'CCGG_A

Cuts at:      0      84     239     606    1239 
   Size:         84     155     367     633

  Fragments arranged by size: 

                 633     367     155      84
 
BspHI T'CATG_A

Cuts at:      0     635    1239 
   Size:        635     604
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1098    1151    1239 
   Size:       1098      53      88

  Fragments arranged by size: 

                1098      88      53
 
BsrFI r'CCGG_y

Cuts at:      0     644    1239 
   Size:        644     595
 
BssSI C'ACGA_G

Cuts at:      0     205    1239 
   Size:        205    1034
 
BstBI TT'CG_AA

Cuts at:      0     339    1239 
   Size:        339     900
 
BstYI r'GATC_y

Cuts at:      0      81    1003    1239 
   Size:         81     922     236

  Fragments arranged by size: 

                 922     236      81
 
BtsI GCAGTG_nn'

Cuts at:      0      62     619    1239 
   Size:         62     557     620

  Fragments arranged by size: 

                 620     557      62
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1152    1187    1239 
   Size:       1152      35      52

  Fragments arranged by size: 

                1152      52      35
 
DraI TTT'AAA

Cuts at:      0     459    1239 
   Size:        459     780
 
EaeI y'GGCC_r

Cuts at:      0     920    1202    1239 
   Size:        920     282      37

  Fragments arranged by size: 

                 920     282      37
 
EarI CTCTTCn'nnn_

Cuts at:      0     490    1239 
   Size:        490     749
 
EcoICRI GAG'CTC

Cuts at:      0     945    1239 
   Size:        945     294
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      57     496     553     721     758    1239 
   Size:         57     439      57     168      37     481

  Fragments arranged by size: 

                 481     439     168      57      57      37
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1046    1239 
   Size:       1046     193
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     454     486     494     526     995    1027    1239 
   Size:        454      32       8      32     469      32     212

  Fragments arranged by size: 

                 469     454     212      32      32      32       8
 
HincII GTy'rAC

Cuts at:      0     102    1239 
   Size:        102    1137
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     801    1239 
   Size:        801     438
 
MscI TGG'CCA

Cuts at:      0     922    1204    1239 
   Size:        922     282      35

  Fragments arranged by size: 

                 922     282      35
 
MslI CAynn'nnrTG

Cuts at:      0     572    1239 
   Size:        572     667
 
MspA1I CmG'CkG

Cuts at:      0     448    1239 
   Size:        448     791
 
NspI r_CATG'y

Cuts at:      0    1080    1239 
   Size:       1080     159
 
PasI CC'CwG_GG

Cuts at:      0     143     903    1042    1239 
   Size:        143     760     139     197

  Fragments arranged by size: 

                 760     197     143     139
 
PpuMI rG'GwC_Cy

Cuts at:      0    1046    1239 
   Size:       1046     193
 
SacI G_AGCT'C

Cuts at:      0     947    1239 
   Size:        947     292
 
SmlI C'TyrA_G

Cuts at:      0     286    1239 
   Size:        286     953
 
StyI C'CwwG_G

Cuts at:      0     264    1239 
   Size:        264     975
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     458    1239 
   Size:        458     781
 
XbaI T'CTAG_A

Cuts at:      0    1191    1239 
   Size:       1191      48
 

 Enzymes that do cut:

     AcuI     AgeI     AhdI     AleI     AlfI    ApaLI    BamHI     BanI
    BanII     BbsI     BclI    BglII Bme1580I     BmrI     BpmI   Bpu10I
    BpuEI    BsaAI    BsaWI    BseYI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrFI    BssSI    BstBI    BstYI     BtsI    CspCI
     DraI     EaeI     EarI  EcoICRI  Eco57MI EcoO109I    Hin4I   HincII
     MmeI     MscI     MslI   MspA1I     NspI     PasI    PpuMI     SacI
     SmlI     StyI    TaqII     XbaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AloI    AlwNI     ApaI     ApoI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI     BbeI    BbvCI     BcgI    BciVI    BfrBI     BglI
     BlpI    BmgBI     BmtI     BplI     BsaI    BsaBI    BsaHI    BsaXI
    BseRI     BsgI    BsiEI    BsiWI    BsmBI    BsrBI    BsrDI    BsrGI
   BssHII   BstAPI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI   DraIII     DrdI     EagI     EciI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
     TatI     TstI  Tth111I     XcmI     XhoI     XmaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com