Restriction map for pF1KB4576
 (Linear) (Six-base) MAPSORT of: KIBB4576.seq  Check: 3687  from: 1  to: 567



KIBB4576.orf

 With 182 enzymes: SgfI * 

                        February 15, 2010 17:49  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      85     567 
   Size:         85     482
 
AlwNI CAG_nnn'CTG

Cuts at:      0     191     567 
   Size:        191     376
 
BamHI G'GATC_C

Cuts at:      0     479     567 
   Size:        479      88
 
BanI G'GyrC_C

Cuts at:      0     322     567 
   Size:        322     245
 
BanII G_rGCy'C

Cuts at:      0     185     285     567 
   Size:        185     100     282

  Fragments arranged by size: 

                 282     185     100
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      73     558     567 
   Size:         73     485       9

  Fragments arranged by size: 

                 485      73       9
 
BbvCI CC'TCA_GC

Cuts at:      0     331     567 
   Size:        331     236
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      63      97     567 
   Size:         63      34     470

  Fragments arranged by size: 

                 470      63      34
 
BclI T'GATC_A

Cuts at:      0     344     567 
   Size:        344     223
 
BglI GCCn_nnn'nGGC

Cuts at:      0     180     235     317     567 
   Size:        180      55      82     250

  Fragments arranged by size: 

                 250     180      82      55
 
Bme1580I G_kGCm'C

Cuts at:      0     327     567 
   Size:        327     240
 
BmtI G_CTAG'C

Cuts at:      0     454     567 
   Size:        454     113
 
Bpu10I CC'TnA_GC

Cuts at:      0     184     331     567 
   Size:        184     147     236

  Fragments arranged by size: 

                 236     184     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     272     302     567 
   Size:        272      30     265

  Fragments arranged by size: 

                 272     265      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     319     567 
   Size:        319     248
 
BsiEI CG_ry'CG

Cuts at:      0     549     567 
   Size:        549      18
 
BsiHKAI G_wGCw'C

Cuts at:      0     215     567 
   Size:        215     352
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     207     451     567 
   Size:        207     244     116

  Fragments arranged by size: 

                 244     207     116
 
Bsp1286I G_dGCh'C

Cuts at:      0     185     215     285     327     567 
   Size:        185      30      70      42     240

  Fragments arranged by size: 

                 240     185      70      42      30
 
BsrDI GCAATG_nn'

Cuts at:      0     153     224     567 
   Size:        153      71     343

  Fragments arranged by size: 

                 343     153      71
 
BssSI C'ACGA_G

Cuts at:      0      52     567 
   Size:         52     515
 
BstEII G'GTnAC_C

Cuts at:      0     288     567 
   Size:        288     279
 
BstYI r'GATC_y

Cuts at:      0      77     479     567 
   Size:         77     402      88

  Fragments arranged by size: 

                 402      88      77
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     103     567 
   Size:        103     464
 
BtsI GCAGTG_nn'

Cuts at:      0     440     567 
   Size:        440     127
 
EaeI y'GGCC_r

Cuts at:      0       5     546     567 
   Size:          5     541      21

  Fragments arranged by size: 

                 541      21       5
 
EagI C'GGCC_G

Cuts at:      0     546     567 
   Size:        546      21
 
EarI CTCTTCn'nnn_

Cuts at:      0     220     567 
   Size:        220     347
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     298     567 
   Size:        298     269
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      85     567 
   Size:         85     482
 
HaeII r_GCGC'y

Cuts at:      0     241     567 
   Size:        241     326
 
HindIII A'AGCT_T

Cuts at:      0     407     567 
   Size:        407     160
 
NheI G'CTAG_C

Cuts at:      0     450     567 
   Size:        450     117
 
PasI CC'CwG_GG

Cuts at:      0     474     567 
   Size:        474      93
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     432     567 
   Size:        432     135
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     346     378     567 
   Size:        346      32     189

  Fragments arranged by size: 

                 346     189      32
 
SapI GCTCTTCn'nnn_

Cuts at:      0     220     567 
   Size:        220     347
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     272     304     567 
   Size:        272      32     263

  Fragments arranged by size: 

                 272     263      32
 

 Enzymes that do cut:

     AcuI    AlwNI    BamHI     BanI    BanII     BbsI    BbvCI     BcgI
     BclI     BglI Bme1580I     BmtI   Bpu10I    BsaXI    BseRI    BsiEI
  BsiHKAI    BsmBI Bsp1286I    BsrDI    BssSI   BstEII    BstYI    BtgZI
     BtsI     EaeI     EagI     EarI     EciI  Eco57MI    HaeII  HindIII
     NheI     PasI    PflMI     PsrI     SapI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BbeI    BciVI
    BfrBI    BglII     BlpI    BmgBI     BmrI     BplI     BpmI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI     BsgI    BsiWI
     BsmI    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII
   BstAPI    BstBI    BstXI  BstZ17I   Bsu36I     BtgI     ClaI    CspCI
     DraI   DraIII     DrdI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    Hin4I   HincII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NotI     NruI     NsiI     NspI
     PacI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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