Restriction map for pF1KB7732
##################################
# NO CUT ENZYMES (  144 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AciI        
AclI        AcyI        AfaI        AflII       AhdI        
Alw44I      AlwNI       AocI        ApaI        ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AvaI        AviII       AvrII       BalI        BamHI       
BanI        BbeI        BbrPI       BcgI        BciVI       
BclI        BcnI        BfaI        BfrI        BglI        
BlnI        BsaAI       BsaHI       BseAI       BsgI        
BsiEI       BsiWI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspLU11I    
BsrBI       BsrGI       BssHII      BssSI       BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
Bsu36I      ClaI        CpoI        DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EclXI       
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRV       
EcoT22I     FauI        FbaI        FseI        FspI        
HgaI        Hin1I       HincII      HindII      HpaI        
KasI        KpnI        KspI        MaeI        MluI        
MluNI       MroI        MscI        MspA1I      MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflMI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RsaI        
RsrII       SacII       SalI        SbfI        ScaI        
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        Van91I      
XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccI         1312  GTATAC               GT^[AC][GT]AC
AflIII        620  ACGTGT               A^C[GA][CT]GT
AgeI         1316  ACCGGT               A^CCGGT
Aor51HI      1388  AGCGCT               AGC^GCT
AspHI         360  GAGCTC               G[AT]GC[AT]^C
AspI            3  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI         496  CCTCAGC              CCTCAGC(-5/-2)
BbvI           69  GCTGC                GCAGC(8/12)
BglII         305  AGATCT               A^GATCT
BlpI          509  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      509  GCTCAGC              GC^T[ACGT]AGC
BsaBI         725  GATGTAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         1191  GAGACC               GGTCTC(1/5)
BsaWI        1316  ACCGGT               [AT]^CCGG[AT]
BsiHKAI       360  GAGCTC               G[AT]GC[AT]^C
BspHI         810  TCATGA               T^CATGA
BspMI         128  ACCTGC               ACCTGC(4/8)
BsrFI        1316  ACCGGT               [GA]^CCGG[CT]
Bst1107I     1312  GTATAC               GTA^TAC
BstZ17I      1312  GTATAC               GTA^TAC
Cac8I         299  GCCAGC               GC[ACGT]^[ACGT]GC
CelII         509  GCTCAGC              GC^T[ACGT]AGC
Cfr10I       1316  ACCGGT               [GA]^CCGG[CT]
DraI         1434  TTTAAA               TTT^AAA
DraII         799  AGGGCCT              [GA]G^G[ACGT]CC[CT]
Eco47III     1388  AGCGCT               AGC^GCT
EcoO109I      799  AGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoT14I        11  CCAAGG               C^C[AT][AT]GG
Fnu4HI         69  GCTGC                GC^[ACGT]GC
HapII        1317  CCGG                 C^CGG
HpaII        1317  CCGG                 C^CGG
HphI          382  GGTGA                GGTGA(8/7)
ItaI           69  GCTGC                GC^[ACGT]GC
MaeII         620  ACGT                 A^CGT
MamI          725  GATGTAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          444  CAATTG               C^AATTG
MspI         1317  CCGG                 C^CGG
MunI          444  CAATTG               C^AATTG
MwoI           63  GCTGGGGCTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV         125  GGAACC               GG[ACGT]^[ACGT]CC
NspI          790  GCATGT               [GA]CATG^[CT]
PflFI           3  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        1316  ACCGGT               A^CCGGT
RcaI          810  TCATGA               T^CATGA
SacI          360  GAGCTC               GAGCT^C
StyI           11  CCAAGG               C^C[AT][AT]GG
TaqI           99  TCGA                 T^CGA
TseI           69  GCTGC                G^C[AT]GC
Tth111I         3  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I        99  TCGA                 T^CGA
XcmI          280  CCACATGAAGGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

BanII         360  GAGCTC               G[GA]GC[CT]^C
             1175  GAGCCC              
BmyI          360  GAGCTC               G[AGT]GC[ACT]^C
             1175  GAGCCC              
BseRI          55  GAGGAG               GAGGAG(10/8)
               58  GAGGAG              
Bsp1286I      360  GAGCTC               G[AGT]GC[ACT]^C
             1175  GAGCCC              
BsrDI         748  CATTGC               GCAATG(2/0)
             1264  CATTGC              
BstYI         242  AGATCC               [GA]^GATC[CT]
              305  AGATCT              
CfoI         1389  GCGC                 GCG^C
             1401  GCGC                
HaeII        1388  AGCGCT               [GA]GCGC^[CT]
             1400  GGCGCT              
HhaI         1389  GCGC                 GCG^C
             1401  GCGC                
HinP1I       1389  GCGC                 G^CGC
             1401  GCGC                
HindIII       549  AAGCTT               A^AGCTT
             1306  AAGCTT              
MflI          242  AGATCC               [GA]^GATC[CT]
              305  AGATCT              
SmlI          341  CTCAAG               C^T[CT][GA]AG
             1236  CTCAAG              
StuI          337  AGGCCT               AGG^CCT
             1232  AGGCCT              
TfiI         1248  GAATC                G^A[AT]TC
             1407  GAATC               
XhoII         242  AGATCC               [GA]^GATC[CT]
              305  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   47 enzymes) #
#########################################

AcsI        AluI        AlwI        ApoI        Asp700I     
AvaII       BbsI        BpmI        BpuAI       BsaJI       
BsiYI       BslI        BsmAI       BsrI        BssKI       
BstF5I      BstNI       Cfr13I      DdeI        DpnII       
EarI        EcoRI       EcoRII      FokI        HaeIII      
HinfI       Ksp632I     MaeIII      MboI        MboII       
MnlI        MseI        MslI        MvaI        NdeII       
NlaIII      PleI        SapI        Sau3AI      Sau96I      
ScrFI       SfaNI       Tru9I       Tsp45I      Tsp509I     
TspRI       XmnI        
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