Restriction map for
pF1KB7732
(Circular) (Six-base) MAPSORT of: pF1KB7732.seq Check: 9962 from: 1 to: 4559
pF1KB7732 4559 bp
With 182 enzymes: SgfI *
February 15, 2010 17:50 ..
AccI GT'mk_AC
Cuts at: 1421 1597 1421
Size: 176 4383
Acc65I G'GTAC_C
Cuts at: 1575 1575
Size: 4559
AclI AA'CG_TT
Cuts at: 4399 4399
Size: 4559
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 213 561 813 916 1149 2384 2816 3563 3831
Size: 348 252 103 233 1235 432 747 268
Cuts at: 3831 213
Size: 941
Fragments arranged by size:
1235 941 747 432 348 268 252 233
103
AfeI AGC'GCT
Cuts at: 1498 1498
Size: 4559
AflIII A'CryG_T
Cuts at: 728 1911 3015 3992 728
Size: 1183 1104 977 1295
Fragments arranged by size:
1295 1183 1104 977
AgeI A'CCGG_T
Cuts at: 1424 2947 1424
Size: 1523 3036
AlwNI CAG_nnn'CTG
Cuts at: 1864 3431 1864
Size: 1567 2992
ApaLI G'TGCA_C
Cuts at: 3329 3329
Size: 4559
ApoI r'AATT_y
Cuts at: 336 742 947 1563 3912 4168 336
Size: 406 205 616 2349 256 727
Fragments arranged by size:
2349 727 616 406 256 205
AseI AT'TA_AT
Cuts at: 20 20
Size: 4559
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4559
AvaI C'yCGr_G
Cuts at: 1579 1579
Size: 4559
BamHI G'GATC_C
Cuts at: 1584 1584
Size: 4559
BanI G'GyrC_C
Cuts at: 1575 1989 2237 2272 1575
Size: 414 248 35 3862
Fragments arranged by size:
3862 414 248 35
BanII G_rGCy'C
Cuts at: 472 1287 1573 2603 472
Size: 815 286 1030 2428
Fragments arranged by size:
2428 1030 815 286
BbeI G_GCGC'C
Cuts at: 1993 2241 1993
Size: 248 4311
BbsI GAAGACnn'nnnn_
Cuts at: 147 373 1490 147
Size: 226 1117 3216
Fragments arranged by size:
3216 1117 226
BbvCI CC'TCA_GC
Cuts at: 605 605
Size: 4559
BciVI GTATCCnnnnn_n'
Cuts at: 2457 3218 4559 2457
Size: 761 1341 2457
Fragments arranged by size:
2457 1341 761
BclI T'GATC_A
Cuts at: 2079 2079
Size: 4559
BglII A'GATC_T
Cuts at: 413 2074 4111 413
Size: 1661 2037 861
Fragments arranged by size:
2037 1661 861
BlpI GC'TnA_GC
Cuts at: 618 1671 618
Size: 1053 3506
Bme1580I G_kGCm'C
Cuts at: 2184 2277 3333 2184
Size: 93 1056 3410
Fragments arranged by size:
3410 1056 93
BmgBI CAC'GTC
Cuts at: 729 729
Size: 4559
BmrI ACTGGGnnnn_n'
Cuts at: 1070 1771 1852 2187 4331 1070
Size: 701 81 335 2144 1298
Fragments arranged by size:
2144 1298 701 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 730 2926 730
Size: 2196 2363
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 809 1229 241
Size: 568 420 3571
Fragments arranged by size:
3571 568 420
Bpu10I CC'TnA_GC
Cuts at: 605 605
Size: 4559
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 434 1329 1735 2614 3106 3404 434
Size: 895 406 879 492 298 1589
Fragments arranged by size:
1589 895 879 492 406 298
BsaI GGTCTCn'nnnn_
Cuts at: 1293 4263 1293
Size: 2970 1589
BsaAI yAC'GTr
Cuts at: 1912 1912
Size: 4559
BsaBI GATnn'nnATC
Cuts at: 6 837 2073 4110 6
Size: 831 1236 2037 455
Fragments arranged by size:
2037 1236 831 455
BsaHI Gr'CG_yC
Cuts at: 1990 2238 2940 4436 1990
Size: 248 702 1496 2113
Fragments arranged by size:
2113 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1424 1748 2269 2947 3221 3368 3779 1424
Size: 324 521 678 274 147 411 2204
Fragments arranged by size:
2204 678 521 411 324 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 283 313 283
Size: 30 4529
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 178 181 178
Size: 3 4556
BseYI C'CCAG_C
Cuts at: 171 1984 2742 3319 3815 171
Size: 1813 758 577 496 915
Fragments arranged by size:
1813 915 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2101 2147 3355 104
Size: 1997 46 1208 1308
Fragments arranged by size:
1997 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 472 1573 2351 2541 3333 472
Size: 1101 778 190 792 1698
Fragments arranged by size:
1698 1101 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 472 1287 1573 2184 2277 2351 2541 2603 3333
Size: 815 286 611 93 74 190 62 730
Cuts at: 3333 472
Size: 1698
Fragments arranged by size:
1698 815 730 611 286 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3779 3779
Size: 4559
BspHI T'CATG_A
Cuts at: 918 3735 918
Size: 2817 1742
BspMI ACCTGCnnnn'nnnn_
Cuts at: 245 1609 1823 2125 2506 245
Size: 1364 214 302 381 2298
Fragments arranged by size:
2298 1364 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2851 2905 4389 4554 50
Size: 2801 54 1484 165 55
Fragments arranged by size:
2801 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 855 1371 2471 855
Size: 516 1100 2943
Fragments arranged by size:
2943 1100 516
BsrFI r'CCGG_y
Cuts at: 1424 2557 2738 2947 1424
Size: 1133 181 209 3036
Fragments arranged by size:
3036 1133 209 181
BssSI C'ACGA_G
Cuts at: 2830 3188 2830
Size: 358 4201
BstAPI GCAn_nnn'nTGC
Cuts at: 1936 1936
Size: 4559
BstBI TT'CG_AA
Cuts at: 2920 2920
Size: 4559
BstYI r'GATC_y
Cuts at: 1 350 413 1584 2051 2074 2409 2655 3667
Size: 349 63 1171 467 23 335 246 1012
Cuts at: 3667 3753 3765 4111 1
Size: 86 12 346 449
Fragments arranged by size:
1171 1012 467 449 349 346 335 246
86 63 23 12
BstZ17I GTA'TAC
Cuts at: 1422 1422
Size: 4559
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2509 2690 2853 2509
Size: 181 163 4215
Fragments arranged by size:
4215 181 163
BtsI GCAGTG_nn'
Cuts at: 134 155 1802 134
Size: 21 1647 2891
Fragments arranged by size:
2891 1647 21
DraI TTT'AAA
Cuts at: 1544 1558 1544
Size: 14 4545
DrdI GACnn_nn'nnGTC
Cuts at: 2265 3123 2265
Size: 858 3701
EaeI y'GGCC_r
Cuts at: 2144 2318 2709 2736 4081 2144
Size: 174 391 27 1345 2622
Fragments arranged by size:
2622 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2144 2144
Size: 4559
EarI CTCTTCn'nnn_
Cuts at: 1153 1237 1321 2582 2792 1153
Size: 84 84 1261 210 2920
Fragments arranged by size:
2920 1261 210 84 84
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2767 3077 3223 4369 2767
Size: 310 146 1146 2957
Fragments arranged by size:
2957 1146 310 146
EcoICRI GAG'CTC
Cuts at: 470 1571 470
Size: 1101 3458
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 213 241 561 809 813 916 1149 1229 2384
Size: 28 320 248 4 103 233 80 1155
Cuts at: 2384 2816 3563 3831 213
Size: 432 747 268 941
Fragments arranged by size:
1155 941 747 432 320 268 248 233
103 80 28 4
EcoO109I rG'GnC_Cy
Cuts at: 908 1698 908
Size: 790 3769
EcoRI G'AATT_C
Cuts at: 336 742 947 1563 336
Size: 406 205 616 3332
Fragments arranged by size:
3332 616 406 205
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1527 2578 1527
Size: 1051 3508
FspI TGC'GCA
Cuts at: 2340 2340
Size: 4559
HaeII r_GCGC'y
Cuts at: 1500 1512 1993 2241 3263 4242 1500
Size: 12 481 248 1022 979 1817
Fragments arranged by size:
1817 1022 979 481 248 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 335 367 484 516 744 776 984 1016 2401
Size: 32 117 32 228 32 208 32 1385
Cuts at: 2401 2431 2433 2463 335
Size: 30 2 30 2431
Fragments arranged by size:
2431 1385 228 208 117 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1598 1598
Size: 4559
HindIII A'AGCT_T
Cuts at: 657 1414 657
Size: 757 3802
KasI G'GCGC_C
Cuts at: 1989 2237 1989
Size: 248 4311
KpnI G_GTAC'C
Cuts at: 1579 1579
Size: 4559
MfeI C'AATT_G
Cuts at: 552 552
Size: 4559
MluI A'CGCG_T
Cuts at: 3992 3992
Size: 4559
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 378 3230 3414 378
Size: 2852 184 1523
Fragments arranged by size:
2852 1523 184
MscI TGG'CCA
Cuts at: 2320 2320
Size: 4559
MslI CAynn'nnrTG
Cuts at: 112 638 972 1224 1392 1476 2548 2675 112
Size: 526 334 252 168 84 1072 127 1996
Fragments arranged by size:
1996 1072 526 334 252 168 127 84
MspA1I CmG'CkG
Cuts at: 1670 1984 2344 3357 3602 1670
Size: 314 360 1013 245 2627
Fragments arranged by size:
2627 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2740 2740
Size: 4559
NarI GG'CG_CC
Cuts at: 1990 2238 1990
Size: 248 4311
NgoMIV G'CCGG_C
Cuts at: 2738 2738
Size: 4559
NspI r_CATG'y
Cuts at: 902 1612 2112 3019 902
Size: 710 500 907 2442
Fragments arranged by size:
2442 907 710 500
PciI A'CATG_T
Cuts at: 3015 3015
Size: 4559
PmeI GTTT'AAAC
Cuts at: 1558 1558
Size: 4559
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3698 3730 3698
Size: 32 4527
PsiI TTA'TAA
Cuts at: 887 1307 887
Size: 420 4139
PstI C_TGCA'G
Cuts at: 1606 2291 1606
Size: 685 3874
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4559
PvuII CAG'CTG
Cuts at: 1984 2344 1984
Size: 360 4199
RsrII CG'GwC_CG
Cuts at: 2754 2754
Size: 4559
SacI G_AGCT'C
Cuts at: 472 1573 472
Size: 1101 3458
SalI G'TCGA_C
Cuts at: 1596 1596
Size: 4559
SapI GCTCTTCn'nnn_
Cuts at: 1153 1237 1321 2582 2792 1153
Size: 84 84 1261 210 2920
Fragments arranged by size:
2920 1261 210 84 84
SbfI CC_TGCA'GG
Cuts at: 1606 1606
Size: 4559
ScaI AGT'ACT
Cuts at: 4076 4076
Size: 4559
SfcI C'TryA_G
Cuts at: 33 1602 2287 3280 3471 33
Size: 1569 685 993 191 1121
Fragments arranged by size:
1569 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1991 2239 1991
Size: 248 4311
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4559
SmaI CCC'GGG
Cuts at: 1581 1581
Size: 4559
SmlI C'TyrA_G
Cuts at: 449 1344 1714 2629 3121 3383 449
Size: 895 370 915 492 262 1625
Fragments arranged by size:
1625 915 895 492 370 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4559
SphI G_CATG'C
Cuts at: 1612 2112 1612
Size: 500 4059
StuI AGG'CCT
Cuts at: 447 1342 447
Size: 895 3664
StyI C'CwwG_G
Cuts at: 119 1693 2047 119
Size: 1574 354 2631
Fragments arranged by size:
2631 1574 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2142 2275 2734 2943 2142
Size: 133 459 209 3758
Fragments arranged by size:
3758 459 209 133
TatI w'GTAC_w
Cuts at: 4074 4074
Size: 4559
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4076 4108 4076
Size: 32 4527
Tth111I GACn'n_nGTC
Cuts at: 114 2356 114
Size: 2242 2317
XbaI T'CTAG_A
Cuts at: 1590 1590
Size: 4559
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 395 2054 395
Size: 1659 2900
XmaI C'CCGG_G
Cuts at: 1579 1579
Size: 4559
XmnI GAAnn'nnTTC
Cuts at: 799 967 1162 799
Size: 168 195 4196
Fragments arranged by size:
4196 195 168
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BstZ17I BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PmeI PpiI PsiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BglI BmtI BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI Bsu36I
BtgI ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HpaI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI