Restriction map for pF1KB7732
 (Linear) (Six-base) MAPSORT of: KIBB7732.seq  Check: 6653  from: 1  to: 1446



KIBB7732.orf

 With 182 enzymes: SgfI * 

                        February 15, 2010 17:50  ..

AccI GT'mk_AC

Cuts at:      0    1313    1446 
   Size:       1313     133
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     105     453     705     808    1041    1446 
   Size:        105     348     252     103     233     405

  Fragments arranged by size: 

                 405     348     252     233     105     103
 
AfeI AGC'GCT

Cuts at:      0    1390    1446 
   Size:       1390      56
 
AflIII A'CryG_T

Cuts at:      0     620    1446 
   Size:        620     826
 
AgeI A'CCGG_T

Cuts at:      0    1316    1446 
   Size:       1316     130
 
ApoI r'AATT_y

Cuts at:      0     228     634     839    1446 
   Size:        228     406     205     607

  Fragments arranged by size: 

                 607     406     228     205
 
BanII G_rGCy'C

Cuts at:      0     364    1179    1446 
   Size:        364     815     267

  Fragments arranged by size: 

                 815     364     267
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      39     265    1382    1446 
   Size:         39     226    1117      64

  Fragments arranged by size: 

                1117     226      64      39
 
BbvCI CC'TCA_GC

Cuts at:      0     497    1446 
   Size:        497     949
 
BglII A'GATC_T

Cuts at:      0     305    1446 
   Size:        305    1141
 
BlpI GC'TnA_GC

Cuts at:      0     510    1446 
   Size:        510     936
 
BmgBI CAC'GTC

Cuts at:      0     621    1446 
   Size:        621     825
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     962    1446 
   Size:        962     484
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     622    1446 
   Size:        622     824
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     133     701    1121    1446 
   Size:        133     568     420     325

  Fragments arranged by size: 

                 568     420     325     133
 
Bpu10I CC'TnA_GC

Cuts at:      0     497    1446 
   Size:        497     949
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     326    1221    1446 
   Size:        326     895     225

  Fragments arranged by size: 

                 895     326     225
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1185    1446 
   Size:       1185     261
 
BsaBI GATnn'nnATC

Cuts at:      0     729    1446 
   Size:        729     717
 
BsaWI w'CCGG_w

Cuts at:      0    1316    1446 
   Size:       1316     130
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     175     205    1446 
   Size:        175      30    1241

  Fragments arranged by size: 

                1241     175      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      70      73    1446 
   Size:         70       3    1373

  Fragments arranged by size: 

                1373      70       3
 
BseYI C'CCAG_C

Cuts at:      0      63    1446 
   Size:         63    1383
 
BsiHKAI G_wGCw'C

Cuts at:      0     364    1446 
   Size:        364    1082
 
Bsp1286I G_dGCh'C

Cuts at:      0     364    1179    1446 
   Size:        364     815     267

  Fragments arranged by size: 

                 815     364     267
 
BspHI T'CATG_A

Cuts at:      0     810    1446 
   Size:        810     636
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     137    1446 
   Size:        137    1309
 
BsrDI GCAATG_nn'

Cuts at:      0     747    1263    1446 
   Size:        747     516     183

  Fragments arranged by size: 

                 747     516     183
 
BsrFI r'CCGG_y

Cuts at:      0    1316    1446 
   Size:       1316     130
 
BstYI r'GATC_y

Cuts at:      0     242     305    1446 
   Size:        242      63    1141

  Fragments arranged by size: 

                1141     242      63
 
BstZ17I GTA'TAC

Cuts at:      0    1314    1446 
   Size:       1314     132
 
BtsI GCAGTG_nn'

Cuts at:      0      26      47    1446 
   Size:         26      21    1399

  Fragments arranged by size: 

                1399      26      21
 
DraI TTT'AAA

Cuts at:      0    1436    1446 
   Size:       1436      10
 
EarI CTCTTCn'nnn_

Cuts at:      0    1045    1129    1213    1446 
   Size:       1045      84      84     233

  Fragments arranged by size: 

                1045     233      84      84
 
EcoICRI GAG'CTC

Cuts at:      0     362    1446 
   Size:        362    1084
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     105     133     453     701     705     808    1041    1121 
   Size:        105      28     320     248       4     103     233      80

Cuts at:   1121    1446 
   Size:        325

  Fragments arranged by size: 

                 325     320     248     233     105     103      80      28
                   4
 
EcoO109I rG'GnC_Cy

Cuts at:      0     800    1446 
   Size:        800     646
 
EcoRI G'AATT_C

Cuts at:      0     228     634     839    1446 
   Size:        228     406     205     607

  Fragments arranged by size: 

                 607     406     228     205
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1419    1446 
   Size:       1419      27
 
HaeII r_GCGC'y

Cuts at:      0    1392    1404    1446 
   Size:       1392      12      42

  Fragments arranged by size: 

                1392      42      12
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     227     259     376     408     636     668     876     908 
   Size:        227      32     117      32     228      32     208      32

Cuts at:    908    1446 
   Size:        538

  Fragments arranged by size: 

                 538     228     227     208     117      32      32      32
                  32
 
HindIII A'AGCT_T

Cuts at:      0     549    1306    1446 
   Size:        549     757     140

  Fragments arranged by size: 

                 757     549     140
 
MfeI C'AATT_G

Cuts at:      0     444    1446 
   Size:        444    1002
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     270    1446 
   Size:        270    1176
 
MslI CAynn'nnrTG

Cuts at:      0     530     864    1116    1284    1368    1446 
   Size:        530     334     252     168      84      78

  Fragments arranged by size: 

                 530     334     252     168      84      78
 
NspI r_CATG'y

Cuts at:      0     794    1446 
   Size:        794     652
 
PsiI TTA'TAA

Cuts at:      0     779    1199    1446 
   Size:        779     420     247

  Fragments arranged by size: 

                 779     420     247
 
SacI G_AGCT'C

Cuts at:      0     364    1446 
   Size:        364    1082
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1045    1129    1213    1446 
   Size:       1045      84      84     233

  Fragments arranged by size: 

                1045     233      84      84
 
SmlI C'TyrA_G

Cuts at:      0     341    1236    1446 
   Size:        341     895     210

  Fragments arranged by size: 

                 895     341     210
 
StuI AGG'CCT

Cuts at:      0     339    1234    1446 
   Size:        339     895     212

  Fragments arranged by size: 

                 895     339     212
 
StyI C'CwwG_G

Cuts at:      0      11    1446 
   Size:         11    1435
 
Tth111I GACn'n_nGTC

Cuts at:      0       6    1446 
   Size:          6    1440
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     287    1446 
   Size:        287    1159
 
XmnI GAAnn'nnTTC

Cuts at:      0     691     859    1054    1446 
   Size:        691     168     195     392

  Fragments arranged by size: 

                 691     392     195     168
 

 Enzymes that do cut:

     AccI     AcuI     AfeI   AflIII     AgeI     ApoI    BanII     BbsI
    BbvCI    BglII     BlpI    BmgBI     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaBI    BsaWI    BsaXI    BseRI    BseYI  BsiHKAI
 Bsp1286I    BspHI    BspMI    BsrDI    BsrFI    BstYI  BstZ17I     BtsI
     DraI     EarI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI    HaeII
    Hin4I  HindIII     MfeI     MmeI     MslI     NspI     PsiI     SacI
     SapI     SmlI     StuI     StyI  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI    AflII     AhdI     AleI     AlfI
     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI     BbeI     BcgI    BciVI     BclI
    BfrBI     BglI Bme1580I     BmtI    BsaAI    BsaHI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BsrBI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI   Bsu36I     BtgI    BtgZI     ClaI
    CspCI   DraIII     DrdI     EaeI     EagI     EciI    EcoNI    EcoRV
     FseI     FspI    FspAI   HincII     HpaI     KasI     KpnI     MluI
     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI    TaqII     TatI
     TstI     XbaI     XhoI     XmaI     ZraI
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