Restriction map for
pF1KE1072
(Circular) (Six-base) MAPSORT of: pF1KE1072.seq Check: 4897 from: 1 to: 5426
pF1KE1072 5426 bp
With 182 enzymes: SgfI *
February 15, 2010 17:50 ..
AccI GT'mk_AC
Cuts at: 2464 2464
Size: 5426
Acc65I G'GTAC_C
Cuts at: 2442 2442
Size: 5426
AclI AA'CG_TT
Cuts at: 5266 5266
Size: 5426
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2300 3251 3683 4430 4698 2300
Size: 951 432 747 268 3028
Fragments arranged by size:
3028 951 747 432 268
AfeI AGC'GCT
Cuts at: 1295 2154 1295
Size: 859 4567
AflIII A'CryG_T
Cuts at: 953 1546 2778 3882 4859 953
Size: 593 1232 1104 977 1520
Fragments arranged by size:
1520 1232 1104 977 593
AgeI A'CCGG_T
Cuts at: 3814 3814
Size: 5426
AhdI GACnn_n'nnGTC
Cuts at: 364 1147 364
Size: 783 4643
AleI CACnn'nnGTG
Cuts at: 271 1939 271
Size: 1668 3758
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 903 903
Size: 5426
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 826 858 1552 1584 826
Size: 32 694 32 4668
Fragments arranged by size:
4668 694 32 32
AlwNI CAG_nnn'CTG
Cuts at: 361 460 611 2731 4298 361
Size: 99 151 2120 1567 1489
Fragments arranged by size:
2120 1567 1489 151 99
ApaI G_GGCC'C
Cuts at: 2276 2276
Size: 5426
ApaLI G'TGCA_C
Cuts at: 4196 4196
Size: 5426
ApoI r'AATT_y
Cuts at: 493 732 1111 2430 4779 5035 493
Size: 239 379 1319 2349 256 884
Fragments arranged by size:
2349 1319 884 379 256 239
AseI AT'TA_AT
Cuts at: 20 20
Size: 5426
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5426
AvaI C'yCGr_G
Cuts at: 2156 2446 2156
Size: 290 5136
BamHI G'GATC_C
Cuts at: 182 2072 2451 182
Size: 1890 379 3157
Fragments arranged by size:
3157 1890 379
BanI G'GyrC_C
Cuts at: 1038 1642 2442 2856 3104 3139 1038
Size: 604 800 414 248 35 3325
Fragments arranged by size:
3325 800 604 414 248 35
BanII G_rGCy'C
Cuts at: 1214 2276 2440 3470 1214
Size: 1062 164 1030 3170
Fragments arranged by size:
3170 1062 1030 164
BbeI G_GCGC'C
Cuts at: 2860 3108 2860
Size: 248 5178
BbsI GAAGACnn'nnnn_
Cuts at: 2288 2288
Size: 5426
BciVI GTATCCnnnnn_n'
Cuts at: 3324 4085 5426 3324
Size: 761 1341 3324
Fragments arranged by size:
3324 1341 761
BclI T'GATC_A
Cuts at: 1928 2946 1928
Size: 1018 4408
BglI GCCn_nnn'nGGC
Cuts at: 1760 1760
Size: 5426
BglII A'GATC_T
Cuts at: 2941 4978 2941
Size: 2037 3389
BlpI GC'TnA_GC
Cuts at: 2538 2538
Size: 5426
Bme1580I G_kGCm'C
Cuts at: 313 1796 2276 3051 3144 4200 313
Size: 1483 480 775 93 1056 1539
Fragments arranged by size:
1539 1483 1056 775 480 93
BmgBI CAC'GTC
Cuts at: 956 1236 2199 956
Size: 280 963 4183
Fragments arranged by size:
4183 963 280
BmrI ACTGGGnnnn_n'
Cuts at: 233 837 2274 2638 2719 3054 5198 233
Size: 604 1437 364 81 335 2144 461
Fragments arranged by size:
2144 1437 604 461 364 335 81
BmtI G_CTAG'C
Cuts at: 256 256
Size: 5426
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2247 3793 2247
Size: 1546 3880
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 162 550 950 2399 162
Size: 388 400 1449 3189
Fragments arranged by size:
3189 1449 400 388
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1338 2602 3481 3973 4271 1338
Size: 1264 879 492 298 2493
Fragments arranged by size:
2493 1264 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 333 1316 1377 5130 333
Size: 983 61 3753 629
Fragments arranged by size:
3753 983 629 61
BsaAI yAC'GTr
Cuts at: 2183 2779 2183
Size: 596 4830
BsaBI GATnn'nnATC
Cuts at: 6 1342 2940 4977 6
Size: 1336 1598 2037 455
Fragments arranged by size:
2037 1598 1336 455
BsaHI Gr'CG_yC
Cuts at: 413 2857 3105 3807 5303 413
Size: 2444 248 702 1496 536
Fragments arranged by size:
2444 1496 702 536 248
BsaWI w'CCGG_w
Cuts at: 2075 2615 3136 3814 4088 4235 4646 2075
Size: 540 521 678 274 147 411 2855
Fragments arranged by size:
2855 678 540 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 425 455 826 856 1225 1255 425
Size: 30 371 30 369 30 4596
Fragments arranged by size:
4596 371 369 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1288 1459 2236 1288
Size: 171 777 4478
Fragments arranged by size:
4478 777 171
BseYI C'CCAG_C
Cuts at: 1158 1533 1749 2851 3609 4186 4682 1158
Size: 375 216 1102 758 577 496 1902
Fragments arranged by size:
1902 1102 758 577 496 375 216
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 2155 166
Size: 1989 3437
BsiEI CG_ry'CG
Cuts at: 104 554 2331 2968 3014 4222 104
Size: 450 1777 637 46 1208 1308
Fragments arranged by size:
1777 1308 1208 637 450 46
BsiHKAI G_wGCw'C
Cuts at: 567 1451 1817 2440 3218 3408 4200 567
Size: 884 366 623 778 190 792 1793
Fragments arranged by size:
1793 884 792 778 623 366 190
BsmI GAATG_Cn'
Cuts at: 1469 1469
Size: 5426
Bsp1286I G_dGCh'C
Cuts at: 313 567 1214 1451 1796 1817 2276 2440 3051
Size: 254 647 237 345 21 459 164 611
Cuts at: 3051 3144 3218 3408 3470 4200 313
Size: 93 74 190 62 730 1539
Fragments arranged by size:
1539 730 647 611 459 345 254 237
190 164 93 74 62 21
BspEI T'CCGG_A
Cuts at: 4646 4646
Size: 5426
BspHI T'CATG_A
Cuts at: 791 4602 791
Size: 3811 1615
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2476 2690 2992 3373 2476
Size: 214 302 381 4529
Fragments arranged by size:
4529 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3718 3772 5256 5421 50
Size: 3668 54 1484 165 55
Fragments arranged by size:
3668 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 176 383 3338 176
Size: 207 2955 2264
Fragments arranged by size:
2955 2264 207
BsrFI r'CCGG_y
Cuts at: 1772 3424 3605 3814 1772
Size: 1652 181 209 3384
Fragments arranged by size:
3384 1652 209 181
BssSI C'ACGA_G
Cuts at: 3697 4055 3697
Size: 358 5068
BstAPI GCAn_nnn'nTGC
Cuts at: 2803 2803
Size: 5426
BstBI TT'CG_AA
Cuts at: 3787 3787
Size: 5426
BstEII G'GTnAC_C
Cuts at: 1800 1800
Size: 5426
BstXI CCAn_nnnn'nTGG
Cuts at: 201 351 1905 201
Size: 150 1554 3722
Fragments arranged by size:
3722 1554 150
BstYI r'GATC_y
Cuts at: 1 182 457 2072 2451 2918 2941 3276 3522
Size: 181 275 1615 379 467 23 335 246
Cuts at: 3522 4534 4620 4632 4978 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1615 1012 467 449 379 346 335 275
246 181 86 23 12
BtgI C'CryG_G
Cuts at: 233 852 1196 1646 233
Size: 619 344 450 4013
Fragments arranged by size:
4013 619 450 344
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 903 3376 3557 3720 903
Size: 2473 181 163 2609
Fragments arranged by size:
2609 2473 181 163
BtsI GCAGTG_nn'
Cuts at: 154 545 974 2669 154
Size: 391 429 1695 2911
Fragments arranged by size:
2911 1695 429 391
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1530 1565 1530
Size: 35 5391
DraI TTT'AAA
Cuts at: 2425 2425
Size: 5426
DraIII CAC_nnn'GTG
Cuts at: 1800 1800
Size: 5426
DrdI GACnn_nn'nnGTC
Cuts at: 3132 3990 3132
Size: 858 4568
EaeI y'GGCC_r
Cuts at: 341 551 1121 2328 3011 3185 3576 3603 4948
Size: 210 570 1207 683 174 391 27 1345
Cuts at: 4948 341
Size: 819
Fragments arranged by size:
1345 1207 819 683 570 391 210 174
27
EagI C'GGCC_G
Cuts at: 551 2328 3011 551
Size: 1777 683 2966
Fragments arranged by size:
2966 1777 683
EarI CTCTTCn'nnn_
Cuts at: 811 1174 1828 2156 2344 3449 3659 811
Size: 363 654 328 188 1105 210 2578
Fragments arranged by size:
2578 1105 654 363 328 210 188
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 634 847 3634 3944 4090 5236 634
Size: 213 2787 310 146 1146 824
Fragments arranged by size:
2787 1146 824 310 213 146
EcoICRI GAG'CTC
Cuts at: 2438 2438
Size: 5426
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 162 550 950 2300 2399 3251 3683 4430 4698
Size: 388 400 1350 99 852 432 747 268
Cuts at: 4698 162
Size: 890
Fragments arranged by size:
1350 890 852 747 432 400 388 268
99
EcoO109I rG'GnC_Cy
Cuts at: 358 1163 2166 2382 2565 358
Size: 805 1003 216 183 3219
Fragments arranged by size:
3219 1003 805 216 183
EcoRI G'AATT_C
Cuts at: 732 2430 732
Size: 1698 3728
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3445 3445
Size: 5426
FspI TGC'GCA
Cuts at: 3207 3207
Size: 5426
HaeII r_GCGC'y
Cuts at: 1297 2156 2860 3108 4130 5109 1297
Size: 859 704 248 1022 979 1614
Fragments arranged by size:
1614 1022 979 859 704 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 268 300 350 382 425 457 1014 1046 1048
Size: 32 50 32 43 32 557 32 2
Cuts at: 1048 1080 1133 1165 1324 1356 1707 1739 1861
Size: 32 53 32 159 32 351 32 122
Cuts at: 1861 1893 2111 2143 2197 2229 3268 3298 3300
Size: 32 218 32 54 32 1039 30 2
Cuts at: 3300 3330 268
Size: 30 2364
Fragments arranged by size:
2364 1039 557 351 218 159 122 54
53 50 43 32 32 32 32 32
32 32 32 32 32 32 30 30
2 2
HincII GTy'rAC
Cuts at: 1613 1626 2465 1613
Size: 13 839 4574
Fragments arranged by size:
4574 839 13
HindIII A'AGCT_T
Cuts at: 1493 1493
Size: 5426
KasI G'GCGC_C
Cuts at: 2856 3104 2856
Size: 248 5178
KpnI G_GTAC'C
Cuts at: 2446 2446
Size: 5426
MluI A'CGCG_T
Cuts at: 4859 4859
Size: 5426
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4097 4281 4097
Size: 184 5242
MscI TGG'CCA
Cuts at: 343 1123 3187 343
Size: 780 2064 2582
Fragments arranged by size:
2582 2064 780
MslI CAynn'nnrTG
Cuts at: 271 316 322 1078 1810 1831 1939 2029 3415
Size: 45 6 756 732 21 108 90 1386
Cuts at: 3415 3542 271
Size: 127 2155
Fragments arranged by size:
2155 1386 756 732 127 108 90 45
21 6
MspA1I CmG'CkG
Cuts at: 302 611 2537 2851 3211 4224 4469 302
Size: 309 1926 314 360 1013 245 1259
Fragments arranged by size:
1926 1259 1013 360 314 309 245
NaeI GCC'GGC
Cuts at: 3607 3607
Size: 5426
NarI GG'CG_CC
Cuts at: 2857 3105 2857
Size: 248 5178
NcoI C'CATG_G
Cuts at: 1196 1196
Size: 5426
NdeI CA'TA_TG
Cuts at: 1005 1005
Size: 5426
NgoMIV G'CCGG_C
Cuts at: 3605 3605
Size: 5426
NheI G'CTAG_C
Cuts at: 252 252
Size: 5426
NspI r_CATG'y
Cuts at: 321 579 890 1550 2479 2979 3886 321
Size: 258 311 660 929 500 907 1861
Fragments arranged by size:
1861 929 907 660 500 311 258
PciI A'CATG_T
Cuts at: 1546 3882 1546
Size: 2336 3090
PflMI CCAn_nnn'nTGG
Cuts at: 2069 2069
Size: 5426
PfoI T'CCnGG_A
Cuts at: 178 527 178
Size: 349 5077
PmeI GTTT'AAAC
Cuts at: 2425 2425
Size: 5426
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 826 858 4565 4597 826
Size: 32 3707 32 1655
Fragments arranged by size:
3707 1655 32 32
PpuMI rG'GwC_Cy
Cuts at: 358 1163 358
Size: 805 4621
PspOMI G'GGCC_C
Cuts at: 2272 2272
Size: 5426
PstI C_TGCA'G
Cuts at: 1958 2473 3158 1958
Size: 515 685 4226
Fragments arranged by size:
4226 685 515
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5426
PvuII CAG'CTG
Cuts at: 302 2851 3211 302
Size: 2549 360 2517
Fragments arranged by size:
2549 2517 360
RsrII CG'GwC_CG
Cuts at: 3621 3621
Size: 5426
SacI G_AGCT'C
Cuts at: 2440 2440
Size: 5426
SalI G'TCGA_C
Cuts at: 2463 2463
Size: 5426
SanDI GG'GwC_CC
Cuts at: 1163 1163
Size: 5426
SapI GCTCTTCn'nnn_
Cuts at: 1828 2344 3449 3659 1828
Size: 516 1105 210 3595
Fragments arranged by size:
3595 1105 516 210
SbfI CC_TGCA'GG
Cuts at: 2473 2473
Size: 5426
ScaI AGT'ACT
Cuts at: 4943 4943
Size: 5426
SexAI A'CCwGG_T
Cuts at: 360 360
Size: 5426
SfcI C'TryA_G
Cuts at: 33 471 1954 2469 3154 4147 4338 33
Size: 438 1483 515 685 993 191 1121
Fragments arranged by size:
1483 1121 993 685 515 438 191
SfoI GGC'GCC
Cuts at: 2858 3106 2858
Size: 248 5178
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5426
SmaI CCC'GGG
Cuts at: 2448 2448
Size: 5426
SmlI C'TyrA_G
Cuts at: 1317 2156 2581 3496 3988 4250 1317
Size: 839 425 915 492 262 2493
Fragments arranged by size:
2493 915 839 492 425 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5426
SphI G_CATG'C
Cuts at: 2479 2979 2479
Size: 500 4926
SspI AAT'ATT
Cuts at: 1689 1689
Size: 5426
StuI AGG'CCT
Cuts at: 803 803
Size: 5426
StyI C'CwwG_G
Cuts at: 344 1196 1367 1571 2560 2914 344
Size: 852 171 204 989 354 2856
Fragments arranged by size:
2856 989 852 354 204 171
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 771 3009 3142 3601 3810 771
Size: 2238 133 459 209 2387
Fragments arranged by size:
2387 2238 459 209 133
TatI w'GTAC_w
Cuts at: 4941 4941
Size: 5426
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1225 1257 4943 4975 1225
Size: 32 3686 32 1676
Fragments arranged by size:
3686 1676 32 32
Tth111I GACn'n_nGTC
Cuts at: 3223 3223
Size: 5426
XbaI T'CTAG_A
Cuts at: 2457 2457
Size: 5426
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1116 2921 1116
Size: 1805 3621
XhoI C'TCGA_G
Cuts at: 2156 2156
Size: 5426
XmaI C'CCGG_G
Cuts at: 2446 2446
Size: 5426
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NspI PciI
PflMI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII AscI AvrII BaeI BbvCI BcgI
BfrBI Bpu10I BsiWI BsmBI BsrGI BssHII BstZ17I Bsu36I
ClaI EcoNI EcoRV FseI FspAI HpaI MfeI NotI
NruI NsiI PacI PasI PmlI PshAI PsiI PspXI
PsrI SacII SfiI SgrAI SnaBI SrfI SwaI XmnI
ZraI