Restriction map for pF1KE1590
##################################
# NO CUT ENZYMES (   72 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AflII       
AgeI        AhdI        AocI        Aor51HI     AscI        
AseI        AsnI        Asp718I     AspEI       AvrII       
BcgI        BciVI       BfrI        BglI        BlnI        
BsaBI       BsiWI       Bsp1407I    BspLU11I    BsrDI       
BsrGI       BssHII      Bst1107I    BstBI       BstEII      
BstPI       BstZ17I     Bsu36I      CpoI        DrdI        
Eam1105I    Eco47III    Eco81I      EcoO65I     EcoT22I     
FseI        HpaI        KpnI        KspI        MamI        
MfeI        MluI        MunI        NcoI        NheI        
NruI        NsiI        NspV        PacI        PinAI       
PmeI        PshAI       PshBI       Psp1406I    PvuI        
RsrII       SacII       SalI        ScaI        SexAI       
SfiI        SfuI        SgrAI       SnaBI       SpeI        
SspBI       SwaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccIII       1018  TCCGGA               T^CCGGA
AlwNI        2191  CAGATACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI         2110  GGGCCC               GGGCC^C
AspI          361  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         695  TGCGCA               TGC^GCA
BalI          885  TGGCCA               TGG^CCA
BanI         1143  GGCGCC               G^G[CT][GA]CC
BbeI         1143  GGCGCC               GGCGC^C
BbrPI         982  CACGTG               CAC^GTG
BclI          405  TGATCA               T^GATCA
BglII        1504  AGATCT               A^GATCT
BsaWI        1018  TCCGGA               [AT]^CCGG[AT]
BseAI        1018  TCCGGA               T^CCGGA
BsmBI        1891  GAGACG               CGTCTC(1/5)
BspDI        3205  ATCGAT               AT^CGAT
BspEI        1018  TCCGGA               T^CCGGA
BsrBI        3403  GAGCGG               CCGCTC(-3/-3)
ClaI         3205  ATCGAT               AT^CGAT
DsaI         3494  CCACGG               C^C[GA][CT]GG
EagI          534  CGGCCG               C^GGCCG
EclXI         534  CGGCCG               C^GGCCG
Eco52I        534  CGGCCG               C^GGCCG
EcoRI        1215  GAATTC               G^AATTC
FbaI          405  TGATCA               T^GATCA
FspI          695  TGCGCA               TGC^GCA
HindIII      3164  AAGCTT               A^AGCTT
KasI         1143  GGCGCC               G^GCGCC
MluNI         885  TGGCCA               TGG^CCA
MroI         1018  TCCGGA               T^CCGGA
MscI          885  TGGCCA               TGG^CCA
NaeI          567  GCCGGC               GCC^GGC
NarI         1143  GGCGCC               GG^CGCC
NdeI         2707  CATATG               CA^TATG
NgoMIV        567  GCCGGC               G^CCGGC
NotI          533  GCGGCCGC             GC^GGCCGC
NspI         2535  GCATGC               [GA]CATG^[CT]
PaeR7I       2820  CTCGAG               C^TCGAG
PflFI         361  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI         982  CACGTG               CAC^GTG
PmlI          982  CACGTG               CAC^GTG
SbfI         1560  CCTGCAGG             CCTGCA^GG
SfoI         1143  GGCGCC               GGC^GCC
SphI         2535  GCATGC               GCATG^C
Sse8387I     1560  CCTGCAGG             CCTGCA^GG
Tth111I       361  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         2646  TCTAGA               T^CTAGA
XcmI         3092  CCAACCTTGGCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         2820  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AccII         538  CGCG                 CG^CG
              657  CGCG                
AcyI         1143  GGCGCC               G[GA]^CG[CT]C
             1893  GACGCC              
Alw44I       1059  GTGCAC               G^TGCAC
             3477  GTGCAC              
ApaLI        1059  GTGCAC               G^TGCAC
             3477  GTGCAC              
Asp700I      1211  GAATGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1291  GAAGAGGTTC          
BamHI         255  GGATCC               G^GATCC
             3320  GGATCC              
BbvCI         292  CCTCAGC              CCTCAGC(-5/-2)
              133  GCTGAGG             
BlpI         2331  GCTTAGC              GC^T[ACGT]AGC
             2440  GCTGAGC             
Bpu1102I     2331  GCTTAGC              GC^T[ACGT]AGC
             2440  GCTGAGC             
BsaAI         982  CACGTG               [CT]AC^GT[GA]
             1048  TACGTG              
BsaHI        1143  GGCGCC               G[GA]^CG[CT]C
             1893  GACGCC              
BspMI        1827  ACCTGC               ACCTGC(4/8)
             3303  GCAGGT              
BsrFI         371  ACCGGC               [GA]^CCGG[CT]
              567  GCCGGC              
BssSI        2872  CACGAG               CACGAG(-5/-1)
             2150  CTCGTG              
BstUI         538  CGCG                 CG^CG
              657  CGCG                
BstXI         672  CCAGGAGCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1574  CCATCAGTGTGG        
CelII        2331  GCTTAGC              GC^T[ACGT]AGC
             2440  GCTGAGC             
Cfr10I        371  ACCGGC               [GA]^CCGG[CT]
              567  GCCGGC              
DraI         2728  TTTAAA               TTT^AAA
             2995  TTTAAA              
DraIII       1454  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1876  CACTCAGTG           
EcoRV         850  GATATC               GAT^ATC
              954  GATATC              
HaeII        1143  GGCGCC               [GA]GCGC^[CT]
             2261  AGCGCC              
HgaI          361  GACGC                GACGC(5/10)
             1893  GACGC               
Hin1I        1143  GGCGCC               G[GA]^CG[CT]C
             1893  GACGCC              
HincII        245  GTCAAC               GT[CT]^[GA]AC
             3067  GTTGAC              
HindII        245  GTCAAC               GT[CT]^[GA]AC
             3067  GTTGAC              
MvnI          538  CGCG                 CG^CG
              657  CGCG                
PflMI        2652  CCAGGAAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3231  CCAAAGAATGG         
PpuMI        1700  AGGACCT              [GA]G^G[AT]CC[CT]
             1837  GGGTCCC             
SacI         1671  GAGCTC               GAGCT^C
             2155  GAGCTC              
SspI          832  AATATT               AAT^ATT
             1435  AATATT              
Van91I       2652  CCAGGAAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3231  CCAAAGAATGG         
XmnI         1211  GAATGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1291  GAAGAGGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  105 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        ApoI        AspHI       AvaI        AvaII       
BanII       BbsI        BbvI        BcnI        BfaI        
BmyI        BpmI        BpuAI       BsaI        BsaJI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsmI        BsoBI       
Bsp1286I    BspHI       BsrI        BssKI       BstAPI      
BstF5I      BstNI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       EaeI        
EarI        EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PleI        
PstI        PvuII       RcaI        RsaI        SapI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmaI        SmlI        StuI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII       XmaCI       XmaI        
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