Restriction map for pF1KE1590
 (Linear) (Six-base) MAPSORT of: KIEE1590.seq  Check: 6406  from: 1  to: 3504



KIEE1590.orf

 With 182 enzymes: SgfI * 

                        February 15, 2010 17:50  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    3294    3504 
   Size:       3294     210
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     765     914    1752    2287    2959    3394    3504 
   Size:        765     149     838     535     672     435     110

  Fragments arranged by size: 

                 838     765     672     535     435     149     110
 
AflIII A'CryG_T

Cuts at:      0     500     981    1049    3504 
   Size:        500     481      68    2455

  Fragments arranged by size: 

                2455     500     481      68
 
AleI CACnn'nnGTG

Cuts at:      0    2417    3504 
   Size:       2417    1087
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     709    3504 
   Size:        709    2795
 
AlwNI CAG_nnn'CTG

Cuts at:      0    2196    3504 
   Size:       2196    1308
 
ApaI G_GGCC'C

Cuts at:      0    2114    3504 
   Size:       2114    1390
 
ApaLI G'TGCA_C

Cuts at:      0    1059    3477    3504 
   Size:       1059    2418      27

  Fragments arranged by size: 

                2418    1059      27
 
ApoI r'AATT_y

Cuts at:      0     942    1215    2431    3504 
   Size:        942     273    1216    1073

  Fragments arranged by size: 

                1216    1073     942     273
 
AvaI C'yCGr_G

Cuts at:      0    1538    1832    1842    2820    3487    3504 
   Size:       1538     294      10     978     667      17

  Fragments arranged by size: 

                1538     978     667     294      17      10
 
BamHI G'GATC_C

Cuts at:      0     255    3320    3504 
   Size:        255    3065     184

  Fragments arranged by size: 

                3065     255     184
 
BanI G'GyrC_C

Cuts at:      0    1143    3504 
   Size:       1143    2361
 
BanII G_rGCy'C

Cuts at:      0      82     634    1675    2114    2159    3458    3494    3504 
   Size:         82     552    1041     439      45    1299      36      10

  Fragments arranged by size: 

                1299    1041     552     439      82      45      36      10
            
 
BbeI G_GCGC'C

Cuts at:      0    1147    3504 
   Size:       1147    2357
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     150     365    1007    1969    3504 
   Size:        150     215     642     962    1535

  Fragments arranged by size: 

                1535     962     642     215     150
 
BbvCI CC'TCA_GC

Cuts at:      0     134     293    3504 
   Size:        134     159    3211

  Fragments arranged by size: 

                3211     159     134
 
BclI T'GATC_A

Cuts at:      0     405    3504 
   Size:        405    3099
 
BglII A'GATC_T

Cuts at:      0    1504    3504 
   Size:       1504    2000
 
BlpI GC'TnA_GC

Cuts at:      0    2332    2441    3504 
   Size:       2332     109    1063

  Fragments arranged by size: 

                2332    1063     109
 
Bme1580I G_kGCm'C

Cuts at:      0    1063    2114    3481    3504 
   Size:       1063    1051    1367      23

  Fragments arranged by size: 

                1367    1063    1051      23
 
BmgBI CAC'GTC

Cuts at:      0     606    2407    3186    3504 
   Size:        606    1801     779     318

  Fragments arranged by size: 

                1801     779     606     318
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1451    3504 
   Size:       1451    2053
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2001    2324    2884    3421    3504 
   Size:       2001     323     560     537      83

  Fragments arranged by size: 

                2001     560     537     323      83
 
Bpu10I CC'TnA_GC

Cuts at:      0     134     230     293    1139    3355    3504 
   Size:        134      96      63     846    2216     149

  Fragments arranged by size: 

                2216     846     149     134      96      63
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2722    3352    3504 
   Size:       2722     630     152

  Fragments arranged by size: 

                2722     630     152
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      54    1302    2680    2840    3504 
   Size:         54    1248    1378     160     664

  Fragments arranged by size: 

                1378    1248     664     160      54
 
BsaAI yAC'GTr

Cuts at:      0     984    1050    3504 
   Size:        984      66    2454

  Fragments arranged by size: 

                2454     984      66
 
BsaHI Gr'CG_yC

Cuts at:      0    1144    1894    3504 
   Size:       1144     750    1610

  Fragments arranged by size: 

                1610    1144     750
 
BsaWI w'CCGG_w

Cuts at:      0    1018    3504 
   Size:       1018    2486
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1690    1720    3460    3490    3504 
   Size:       1690      30    1740      30      14

  Fragments arranged by size: 

                1740    1690      30      30      14
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     484     808    1675    1683    2881    3206    3445    3504 
   Size:        484     324     867       8    1198     325     239      59

  Fragments arranged by size: 

                1198     867     484     325     324     239      59       8
            
 
BseYI C'CCAG_C

Cuts at:      0     710    1151    1757    2061    2470    2528    2591    2884 
   Size:        710     441     606     304     409      58      63     293

Cuts at:   2884    2937    3457    3504 
   Size:         53     520      47

  Fragments arranged by size: 

                 710     606     520     441     409     304     293      63
                  58      53      47
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     460     859    1147    1168    3504 
   Size:        460     399     288      21    2336

  Fragments arranged by size: 

                2336     460     399     288      21
 
BsiEI CG_ry'CG

Cuts at:      0     537    3409    3465    3504 
   Size:        537    2872      56      39

  Fragments arranged by size: 

                2872     537      56      39
 
BsiHKAI G_wGCw'C

Cuts at:      0     527    1063    1675    1888    2159    2873    3481    3504 
   Size:        527     536     612     213     271     714     608      23

  Fragments arranged by size: 

                 714     612     608     536     527     271     213      23
            
 
BsmI GAATG_Cn'

Cuts at:      0      93    1813    1974    3504 
   Size:         93    1720     161    1530

  Fragments arranged by size: 

                1720    1530     161      93
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1885    3504 
   Size:       1885    1619
 
Bsp1286I G_dGCh'C

Cuts at:      0      82     527     634    1063    1675    1888    2114    2159 
   Size:         82     445     107     429     612     213     226      45

Cuts at:   2159    2873    3458    3481    3494    3504 
   Size:        714     585      23      13      10

  Fragments arranged by size: 

                 714     612     585     445     429     226     213     107
                  82      45      23      13      10
 
BspEI T'CCGG_A

Cuts at:      0    1018    3504 
   Size:       1018    2486
 
BspHI T'CATG_A

Cuts at:      0     324    1899    2832    3504 
   Size:        324    1575     933     672

  Fragments arranged by size: 

                1575     933     672     324
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1836    3294    3504 
   Size:       1836    1458     210

  Fragments arranged by size: 

                1836    1458     210
 
BsrBI CCG'CTC

Cuts at:      0    3405    3504 
   Size:       3405      99
 
BsrFI r'CCGG_y

Cuts at:      0     371     567    3504 
   Size:        371     196    2937

  Fragments arranged by size: 

                2937     371     196
 
BssSI C'ACGA_G

Cuts at:      0    2150    2872    3504 
   Size:       2150     722     632

  Fragments arranged by size: 

                2150     722     632
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1182    2400    3384    3504 
   Size:       1182    1218     984     120

  Fragments arranged by size: 

                1218    1182     984     120
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     679    1581    3504 
   Size:        679     902    1923

  Fragments arranged by size: 

                1923     902     679
 
BstYI r'GATC_y

Cuts at:      0     255    1504    1584    2801    3320    3504 
   Size:        255    1249      80    1217     519     184

  Fragments arranged by size: 

                1249    1217     519     255     184      80
 
BtgI C'CryG_G

Cuts at:      0    3494    3504 
   Size:       3494      10
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1400    3132    3504 
   Size:       1400    1732     372

  Fragments arranged by size: 

                1732    1400     372
 
BtsI GCAGTG_nn'

Cuts at:      0     477    1130    3366    3504 
   Size:        477     653    2236     138

  Fragments arranged by size: 

                2236     653     477     138
 
ClaI AT'CG_AT

Cuts at:      0    3206    3504 
   Size:       3206     298
 
DraI TTT'AAA

Cuts at:      0    2730    2997    3504 
   Size:       2730     267     507

  Fragments arranged by size: 

                2730     507     267
 
DraIII CAC_nnn'GTG

Cuts at:      0    1459    1881    3504 
   Size:       1459     422    1623

  Fragments arranged by size: 

                1623    1459     422
 
EaeI y'GGCC_r

Cuts at:      0     534     885    1118    2779    3504 
   Size:        534     351     233    1661     725

  Fragments arranged by size: 

                1661     725     534     351     233
 
EagI C'GGCC_G

Cuts at:      0     534    3504 
   Size:        534    2970
 
EarI CTCTTCn'nnn_

Cuts at:      0     532     880    1286    1364    1730    2714    3504 
   Size:        532     348     406      78     366     984     790

  Fragments arranged by size: 

                 984     790     532     406     366     348      78
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     554    3459    3504 
   Size:        554    2905      45

  Fragments arranged by size: 

                2905     554      45
 
EcoICRI GAG'CTC

Cuts at:      0    1673    2157    3504 
   Size:       1673     484    1347

  Fragments arranged by size: 

                1673    1347     484
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     765     914    1752    2001    2287    2324    2884    2959 
   Size:        765     149     838     249     286      37     560      75

Cuts at:   2959    3394    3421    3504 
   Size:        435      27      83

  Fragments arranged by size: 

                 838     765     560     435     286     249     149      83
                  75      37      27
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     233    1102    1770    3504 
   Size:        233     869     668    1734

  Fragments arranged by size: 

                1734     869     668     233
 
EcoO109I rG'GnC_Cy

Cuts at:      0     759    1701    1838    2111    3504 
   Size:        759     942     137     273    1393

  Fragments arranged by size: 

                1393     942     759     273     137
 
EcoRI G'AATT_C

Cuts at:      0    1215    3504 
   Size:       1215    2289
 
EcoRV GAT'ATC

Cuts at:      0     852     956    3504 
   Size:        852     104    2548

  Fragments arranged by size: 

                2548     852     104
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     211    1671    3217    3504 
   Size:        211    1460    1546     287

  Fragments arranged by size: 

                1546    1460     287     211
 
FspI TGC'GCA

Cuts at:      0     697    3504 
   Size:        697    2807
 
HaeII r_GCGC'y

Cuts at:      0    1147    2265    3504 
   Size:       1147    1118    1239

  Fragments arranged by size: 

                1239    1147    1118
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1690    1722    1903    1935    2408    2440    3111    3143 
   Size:       1690      32     181      32     473      32     671      32

Cuts at:   3143    3504 
   Size:        361

  Fragments arranged by size: 

                1690     671     473     361     181      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     247    3069    3504 
   Size:        247    2822     435

  Fragments arranged by size: 

                2822     435     247
 
HindIII A'AGCT_T

Cuts at:      0    3164    3504 
   Size:       3164     340
 
KasI G'GCGC_C

Cuts at:      0    1143    3504 
   Size:       1143    2361
 
MscI TGG'CCA

Cuts at:      0     887    3504 
   Size:        887    2617
 
MslI CAynn'nnrTG

Cuts at:      0     112    1579    1638    1937    2417    2711    3504 
   Size:        112    1467      59     299     480     294     793

  Fragments arranged by size: 

                1467     793     480     299     294     112      59
 
MspA1I CmG'CkG

Cuts at:      0      72     611     773    1155    1749    2520    2937    3504 
   Size:         72     539     162     382     594     771     417     567

  Fragments arranged by size: 

                 771     594     567     539     417     382     162      72
            
 
NaeI GCC'GGC

Cuts at:      0     569    3504 
   Size:        569    2935
 
NarI GG'CG_CC

Cuts at:      0    1144    3504 
   Size:       1144    2360
 
NdeI CA'TA_TG

Cuts at:      0    2708    3504 
   Size:       2708     796
 
NgoMIV G'CCGG_C

Cuts at:      0     567    3504 
   Size:        567    2937
 
NotI GC'GGCC_GC

Cuts at:      0     534    3504 
   Size:        534    2970
 
NspI r_CATG'y

Cuts at:      0    2539    3504 
   Size:       2539     965
 
PasI CC'CwG_GG

Cuts at:      0     122    3504 
   Size:        122    3382
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2658    3237    3504 
   Size:       2658     579     267

  Fragments arranged by size: 

                2658     579     267
 
PfoI T'CCnGG_A

Cuts at:      0    1978    2340    3504 
   Size:       1978     362    1164

  Fragments arranged by size: 

                1978    1164     362
 
PmlI CAC'GTG

Cuts at:      0     984    3504 
   Size:        984    2520
 
PpuMI rG'GwC_Cy

Cuts at:      0    1701    1838    3504 
   Size:       1701     137    1666

  Fragments arranged by size: 

                1701    1666     137
 
PsiI TTA'TAA

Cuts at:      0    3076    3504 
   Size:       3076     428
 
PspOMI G'GGCC_C

Cuts at:      0    2110    3504 
   Size:       2110    1394
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    2553    2585    3504 
   Size:       2553      32     919

  Fragments arranged by size: 

                2553     919      32
 
PstI C_TGCA'G

Cuts at:      0      71     752    1565    3044    3422    3504 
   Size:         71     681     813    1479     378      82

  Fragments arranged by size: 

                1479     813     681     378      82      71
 
PvuII CAG'CTG

Cuts at:      0      72    1155    2520    3504 
   Size:         72    1083    1365     984

  Fragments arranged by size: 

                1365    1083     984      72
 
SacI G_AGCT'C

Cuts at:      0    1675    2159    3504 
   Size:       1675     484    1345

  Fragments arranged by size: 

                1675    1345     484
 
SanDI GG'GwC_CC

Cuts at:      0    1838    3504 
   Size:       1838    1666
 
SapI GCTCTTCn'nnn_

Cuts at:      0     532     880    1730    3504 
   Size:        532     348     850    1774

  Fragments arranged by size: 

                1774     850     532     348
 
SbfI CC_TGCA'GG

Cuts at:      0    1565    3504 
   Size:       1565    1939
 
SfcI C'TryA_G

Cuts at:      0      67     748    1561    2312    3040    3418    3504 
   Size:         67     681     813     751     728     378      86

  Fragments arranged by size: 

                 813     751     728     681     378      86      67
 
SfoI GGC'GCC

Cuts at:      0    1145    3504 
   Size:       1145    2359
 
SmaI CCC'GGG

Cuts at:      0    1540    1834    1844    3504 
   Size:       1540     294      10    1660

  Fragments arranged by size: 

                1660    1540     294      10
 
SmlI C'TyrA_G

Cuts at:      0    2737    2820    3331    3504 
   Size:       2737      83     511     173

  Fragments arranged by size: 

                2737     511     173      83
 
SphI G_CATG'C

Cuts at:      0    2539    3504 
   Size:       2539     965
 
SspI AAT'ATT

Cuts at:      0     834    1437    3504 
   Size:        834     603    2067

  Fragments arranged by size: 

                2067     834     603
 
StuI AGG'CCT

Cuts at:      0    1108    1559    1654    1765    2635    3504 
   Size:       1108     451      95     111     870     869

  Fragments arranged by size: 

                1108     870     869     451     111      95
 
StyI C'CwwG_G

Cuts at:      0    2553    2670    3096    3504 
   Size:       2553     117     426     408

  Fragments arranged by size: 

                2553     426     408     117
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1326    1470    2591    3504 
   Size:       1326     144    1121     913

  Fragments arranged by size: 

                1326    1121     913     144
 
TatI w'GTAC_w

Cuts at:      0    1606    3504 
   Size:       1606    1898
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     469     501    2136    2168    3460    3492    3504 
   Size:        469      32    1635      32    1292      32      12

  Fragments arranged by size: 

                1635    1292     469      32      32      32      12
 
Tth111I GACn'n_nGTC

Cuts at:      0     364    3504 
   Size:        364    3140
 
XbaI T'CTAG_A

Cuts at:      0    2646    3504 
   Size:       2646     858
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    3099    3504 
   Size:       3099     405
 
XhoI C'TCGA_G

Cuts at:      0    2820    3504 
   Size:       2820     684
 
XmaI C'CCGG_G

Cuts at:      0    1538    1832    1842    3504 
   Size:       1538     294      10    1662

  Fragments arranged by size: 

                1662    1538     294      10
 
XmnI GAAnn'nnTTC

Cuts at:      0    1215    1295    3504 
   Size:       1215      80    2209

  Fragments arranged by size: 

                2209    1215      80
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AleI     AlfI    AlwNI     ApaI    ApaLI
     ApoI     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
     BclI    BglII     BlpI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrFI    BssSI   BstAPI    BstXI    BstYI     BtgI
    BtgZI     BtsI     ClaI     DraI   DraIII     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FspI    HaeII    Hin4I   HincII  HindIII     KasI     MscI     MslI
   MspA1I     NaeI     NarI     NdeI   NgoMIV     NotI     NspI     PasI
    PflMI     PfoI     PmlI    PpuMI     PsiI   PspOMI     PsrI     PstI
    PvuII     SacI    SanDI     SapI     SbfI     SfcI     SfoI     SmaI
     SmlI     SphI     SspI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AloI     AscI     AseI    AsiSI    AvrII     BaeI     BcgI    BciVI
    BfrBI     BglI     BmtI     BplI    BsaBI    BsiWI    BsrDI    BsrGI
   BssHII    BstBI   BstEII  BstZ17I   Bsu36I    CspCI     DrdI     FseI
    FspAI     HpaI     KpnI     MfeI     MluI     MmeI     NcoI     NheI
     NruI     NsiI     PacI     PciI     PmeI     PpiI    PshAI    PspXI
     PvuI    RsrII    SacII     SalI     ScaI    SexAI     SfiI     SgfI
    SgrAI    SnaBI     SpeI     SrfI     SwaI     ZraI
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