Restriction map for pF1KE1097
##################################
# NO CUT ENZYMES (   92 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
AscI        AseI        AsnI        Asp718I     AspEI       
AvrII       BalI        BbeI        BbvCI       BciVI       
BclI        BfaI        BfrI        BglII       BlnI        
BlpI        Bpu1102I    BsaBI       BsaHI       BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspHI       
BspLU11I    BsrBI       BsrGI       BssHII      Bst1107I    
BstBI       BstUI       BstZ17I     CelII       ClaI        
CpoI        DraI        DraIII      Eam1105I    EcoRV       
FbaI        Hin1I       KasI        KpnI        KspI        
MaeI        MamI        MfeI        MluI        MluNI       
MscI        MunI        MvnI        NarI        NdeI        
NheI        NotI        NruI        NspV        PacI        
PinAI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacII       SalI        
SbfI        ScaI        SfoI        SfuI        SgrAI       
SnaBI       SpeI        Sse8387I    SspBI       SspI        
SwaI        XbaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccIII       1283  TCCGGA               T^CCGGA
AcsI          413  GAATTC               [GA]^AATT[CT]
Aor51HI      1244  AGCGCT               AGC^GCT
ApoI          413  GAATTC               [GA]^AATT[CT]
Asp700I      1339  GAAATCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII         794  TGCGCA               TGC^GCA
BbrPI        1108  CACGTG               CAC^GTG
BcgI           24  CGATGGAGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI        1108  CACGTG               [CT]AC^GT[GA]
BsaWI        1283  TCCGGA               [AT]^CCGG[AT]
BseAI        1283  TCCGGA               T^CCGGA
BsiEI          14  CGGCCG               CG[GA][CT]^CG
BspEI        1283  TCCGGA               T^CCGGA
BspMI        1232  ACCTGC               ACCTGC(4/8)
BsrDI        1349  GCAATG               GCAATG(2/0)
BssSI        1057  CACGAG               CACGAG(-5/-1)
BstEII       1712  GGTCACC              G^GT[ACGT]ACC
BstPI        1712  GGTCACC              G^GT[ACGT]ACC
EagI           14  CGGCCG               C^GGCCG
EclXI          14  CGGCCG               C^GGCCG
Eco47III     1244  AGCGCT               AGC^GCT
Eco52I         14  CGGCCG               C^GGCCG
EcoO65I      1712  GGTCACC              G^GT[ACGT]ACC
EcoRI         413  GAATTC               G^AATTC
EcoT14I      1733  CCATGG               C^C[AT][AT]GG
EcoT22I       973  ATGCAT               ATGCA^T
FseI          106  GGCCGGCC             GGCCGG^CC
FspI          794  TGCGCA               TGC^GCA
HaeII        1244  AGCGCT               [GA]GCGC^[CT]
HgaI         1043  GACGC                GACGC(5/10)
HincII        541  GTTAAC               GT[CT]^[GA]AC
HindII        541  GTTAAC               GT[CT]^[GA]AC
HindIII       289  AAGCTT               A^AGCTT
HpaI          541  GTTAAC               GTT^AAC
MroI         1283  TCCGGA               T^CCGGA
NcoI         1733  CCATGG               C^CATGG
NsiI          973  ATGCAT               ATGCA^T
PaeR7I       1544  CTCGAG               C^TCGAG
PmaCI        1108  CACGTG               CAC^GTG
PmlI         1108  CACGTG               CAC^GTG
SacI         1183  GAGCTC               GAGCT^C
StuI         1557  AGGCCT               AGG^CCT
StyI         1733  CCATGG               C^C[AT][AT]GG
TaqI         1545  TCGA                 T^CGA
TthHB8I      1545  TCGA                 T^CGA
XhoI         1544  CTCGAG               C^TCGAG
XmnI         1339  GAAATCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

Alw44I        658  GTGCAC               G^TGCAC
             1111  GTGCAC              
ApaLI         658  GTGCAC               G^TGCAC
             1111  GTGCAC              
AspI          267  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1707  GACAGGGTC           
BsgI         1152  CTGCAC               GTGCAG(16/14)
              442  GTGCAG              
BsrFI          16  GCCGGC               [GA]^CCGG[CT]
              107  GCCGGC              
BstAPI        125  GCAGCCGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1113  GCACAGCATGC         
Cfr10I         16  GCCGGC               [GA]^CCGG[CT]
              107  GCCGGC              
DrdI         1273  GACCTCAACGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1478  GACCCCAGGGTC        
EcoNI         564  CCTGGCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              624  CCTCACCCAGG         
MseI          298  TTAA                 T^TAA
              542  TTAA                
NaeI           16  GCCGGC               GCC^GGC
              107  GCCGGC              
NgoMIV         16  GCCGGC               G^CCGGC
              107  GCCGGC              
PflFI         267  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1707  GACAGGGTC           
PleI          241  GACTC                GAGTC(4/5)
             1030  GAGTC               
SapI          558  GCTCTTC              GCTCTTC(1/4)
              780  GCTCTTC             
SexAI         536  ACCAGGT              A^CC[AT]GGT
              896  ACCTGGT             
SfaNI          31  GATGC                GCATC(5/9)
              866  GCATC               
SmaI         1653  CCCGGG               CCC^GGG
             1779  CCCGGG              
SmlI         1123  CTCAAG               C^T[CT][GA]AG
             1544  CTCGAG              
SphI          887  GCATGC               GCATG^C
             1118  GCATGC              
TfiI          168  GATTC                G^A[AT]TC
             1397  GATTC               
Tru9I         298  TTAA                 T^TAA
              542  TTAA                
Tth111I       267  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1707  GACAGGGTC           
XcmI          593  CCACAGCTGGCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1761  CCAGGGTCCCCCTGG     
XmaCI        1653  CCCGGG               C^CCGGG
             1779  CCCGGG              
XmaI         1653  CCCGGG               C^CCGGG
             1779  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   93 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AocI        ApaI        AspHI       AvaI        AvaII       
BamHI       BanI        BanII       BbsI        BbvI        
BcnI        BglI        BmyI        BpmI        BpuAI       
BsaI        BsaJI       BseRI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       BstXI       
BstYI       Bsu36I      Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        Eco81I      EcoO109I    EcoRII      FauI        
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PpuMI       PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfcI        SfiI        TseI        Tsp45I      Tsp509I     
TspRI       Van91I      XhoII       
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