Restriction map for pF1KE1097
 (Linear) (Six-base) MAPSORT of: KIEE1097.seq  Check: 3078  from: 1  to: 1818



KIEE1097.orf

 With 182 enzymes: SgfI * 

                        November 19, 2009 09:54  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     637     768     967    1233    1818 
   Size:        637     131     199     266     585

  Fragments arranged by size: 

                 637     585     266     199     131
 
AfeI AGC'GCT

Cuts at:      0    1246    1818 
   Size:       1246     572
 
AleI CACnn'nnGTG

Cuts at:      0     160    1818 
   Size:        160    1658
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1742    1774    1818 
   Size:       1742      32      44

  Fragments arranged by size: 

                1742      44      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     131     321     423     552    1818 
   Size:        131     190     102     129    1266

  Fragments arranged by size: 

                1266     190     131     129     102
 
ApaI G_GGCC'C

Cuts at:      0     119    1471    1534    1634    1652    1670    1818 
   Size:        119    1352      63     100      18      18     148

  Fragments arranged by size: 

                1352     148     119     100      63      18      18
 
ApaLI G'TGCA_C

Cuts at:      0     658    1111    1818 
   Size:        658     453     707

  Fragments arranged by size: 

                 707     658     453
 
ApoI r'AATT_y

Cuts at:      0     413    1818 
   Size:        413    1405
 
AvaI C'yCGr_G

Cuts at:      0    1544    1653    1779    1818 
   Size:       1544     109     126      39

  Fragments arranged by size: 

                1544     126     109      39
 
BamHI G'GATC_C

Cuts at:      0     324     698     707    1757    1818 
   Size:        324     374       9    1050      61

  Fragments arranged by size: 

                1050     374     324      61       9
 
BanI G'GyrC_C

Cuts at:      0    1072    1222    1369    1818 
   Size:       1072     150     147     449

  Fragments arranged by size: 

                1072     449     150     147
 
BanII G_rGCy'C

Cuts at:      0     119     560    1187    1471    1534    1634    1652    1670 
   Size:        119     441     627     284      63     100      18      18

Cuts at:   1670    1818 
   Size:        148

  Fragments arranged by size: 

                 627     441     284     148     119     100      63      18
                  18
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     260     724    1711    1818 
   Size:        260     464     987     107

  Fragments arranged by size: 

                 987     464     260     107
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      13      47    1818 
   Size:         13      34    1771

  Fragments arranged by size: 

                1771      34      13
 
BfrBI ATG'CAT

Cuts at:      0     975    1818 
   Size:        975     843
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1538    1565    1638    1647    1674    1818 
   Size:       1538      27      73       9      27     144

  Fragments arranged by size: 

                1538     144      73      27      27       9
 
Bme1580I G_kGCm'C

Cuts at:      0     119     662    1115    1225    1266    1374    1471    1534 
   Size:        119     543     453     110      41     108      97      63

Cuts at:   1534    1634    1652    1670    1818 
   Size:        100      18      18     148

  Fragments arranged by size: 

                 543     453     148     119     110     108     100      97
                  63      41      18      18
 
BmgBI CAC'GTC

Cuts at:      0    1140    1818 
   Size:       1140     678
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     264    1818 
   Size:        264    1554
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     495    1818 
   Size:        495    1323
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     298     427     727     836     889    1367    1523    1610 
   Size:        298     129     300     109      53     478     156      87

Cuts at:   1610    1646    1772    1818 
   Size:         36     126      46

  Fragments arranged by size: 

                 478     300     298     156     129     126     109      87
                  53      46      36
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1108    1818 
   Size:       1108     710
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     724    1445    1664    1780    1818 
   Size:        724     721     219     116      38

  Fragments arranged by size: 

                 724     721     219     116      38
 
BsaAI yAC'GTr

Cuts at:      0    1110    1818 
   Size:       1110     708
 
BsaWI w'CCGG_w

Cuts at:      0    1283    1818 
   Size:       1283     535
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     232     262     809     839    1818 
   Size:        232      30     547      30     979

  Fragments arranged by size: 

                 979     547     232      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     415     742     846    1195    1321    1818 
   Size:        415     327     104     349     126     497

  Fragments arranged by size: 

                 497     415     349     327     126     104
 
BseYI C'CCAG_C

Cuts at:      0     364     372    1158    1218    1818 
   Size:        364       8     786      60     600

  Fragments arranged by size: 

                 786     600     364      60       8
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     463    1137    1818 
   Size:        463     674     681

  Fragments arranged by size: 

                 681     674     463
 
BsiEI CG_ry'CG

Cuts at:      0      17    1818 
   Size:         17    1801
 
BsiHKAI G_wGCw'C

Cuts at:      0     479     662    1115    1187    1818 
   Size:        479     183     453      72     631

  Fragments arranged by size: 

                 631     479     453     183      72
 
Bsp1286I G_dGCh'C

Cuts at:      0     119     479     560     662    1115    1187    1225    1266 
   Size:        119     360      81     102     453      72      38      41

Cuts at:   1266    1374    1471    1534    1634    1652    1670    1818 
   Size:        108      97      63     100      18      18     148

  Fragments arranged by size: 

                 453     360     148     119     108     102     100      97
                  81      72      63      41      38      18      18
 
BspEI T'CCGG_A

Cuts at:      0    1283    1818 
   Size:       1283     535
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1241    1818 
   Size:       1241     577
 
BsrDI GCAATG_nn'

Cuts at:      0    1356    1818 
   Size:       1356     462
 
BsrFI r'CCGG_y

Cuts at:      0      16     107    1818 
   Size:         16      91    1711

  Fragments arranged by size: 

                1711      91      16
 
BssSI C'ACGA_G

Cuts at:      0    1057    1818 
   Size:       1057     761
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     131    1119    1818 
   Size:        131     988     699

  Fragments arranged by size: 

                 988     699     131
 
BstEII G'GTnAC_C

Cuts at:      0    1712    1818 
   Size:       1712     106
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     364    1218    1302    1818 
   Size:        364     854      84     516

  Fragments arranged by size: 

                 854     516     364      84
 
BstYI r'GATC_y

Cuts at:      0     324     383     698     707    1757    1818 
   Size:        324      59     315       9    1050      61

  Fragments arranged by size: 

                1050     324     315      61      59       9
 
Bsu36I CC'TnA_GG

Cuts at:      0    1490    1671    1679    1742    1818 
   Size:       1490     181       8      63      76

  Fragments arranged by size: 

                1490     181      76      63       8
 
BtgI C'CryG_G

Cuts at:      0     203     677     702    1733    1818 
   Size:        203     474      25    1031      85

  Fragments arranged by size: 

                1031     474     203      85      25
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      38    1818 
   Size:         38    1780
 
BtsI GCAGTG_nn'

Cuts at:      0     662    1325    1818 
   Size:        662     663     493

  Fragments arranged by size: 

                 663     662     493
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1279    1484    1818 
   Size:       1279     205     334

  Fragments arranged by size: 

                1279     334     205
 
EaeI y'GGCC_r

Cuts at:      0      14     109     200    1818 
   Size:         14      95      91    1618

  Fragments arranged by size: 

                1618      95      91      14
 
EagI C'GGCC_G

Cuts at:      0      14    1818 
   Size:         14    1804
 
EarI CTCTTCn'nnn_

Cuts at:      0      44     184     565     787     959    1818 
   Size:         44     140     381     222     172     859

  Fragments arranged by size: 

                 859     381     222     172     140      44
 
EcoICRI GAG'CTC

Cuts at:      0    1185    1818 
   Size:       1185     633
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     298     427     637     727     768     836     889     967 
   Size:        298     129     210      90      41      68      53      78

Cuts at:    967    1233    1367    1523    1610    1646    1772    1818 
   Size:        266     134     156      87      36     126      46

  Fragments arranged by size: 

                 298     266     210     156     134     129     126      90
                  87      78      68      53      46      41      36
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     568     628    1818 
   Size:        568      60    1190

  Fragments arranged by size: 

                1190     568      60
 
EcoO109I rG'GnC_Cy

Cuts at:      0      54     115     116     318     621     851    1423    1468 
   Size:         54      61       1     202     303     230     572      45

Cuts at:   1468    1477    1486    1530    1531    1630    1631    1648    1649 
   Size:          9       9      44       1      99       1      17       1

Cuts at:   1649    1666    1667    1675    1738    1765    1783    1818 
   Size:         17       1       8      63      27      18      35

  Fragments arranged by size: 

                 572     303     230     202      99      63      61      54
                  45      44      35      27      18      17      17       9
                   9       8       1       1       1       1       1
 
EcoRI G'AATT_C

Cuts at:      0     413    1818 
   Size:        413    1405
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1596    1818 
   Size:       1596     222
 
FseI GG_CCGG'CC

Cuts at:      0     111    1818 
   Size:        111    1707
 
FspI TGC'GCA

Cuts at:      0     796    1818 
   Size:        796    1022
 
HaeII r_GCGC'y

Cuts at:      0    1248    1818 
   Size:       1248     570
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     232     264     809     841     925     957    1749    1781 
   Size:        232      32     545      32      84      32     792      32

Cuts at:   1781    1818 
   Size:         37

  Fragments arranged by size: 

                 792     545     232      84      37      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     543    1818 
   Size:        543    1275
 
HindIII A'AGCT_T

Cuts at:      0     289    1818 
   Size:        289    1529
 
HpaI GTT'AAC

Cuts at:      0     543    1818 
   Size:        543    1275
 
MslI CAynn'nnrTG

Cuts at:      0     160    1172    1300    1818 
   Size:        160    1012     128     518

  Fragments arranged by size: 

                1012     518     160     128
 
MspA1I CmG'CkG

Cuts at:      0     122     131     372     447     598     714     788     924 
   Size:        122       9     241      75     151     116      74     136

Cuts at:    924     930    1725    1818 
   Size:          6     795      93

  Fragments arranged by size: 

                 795     241     151     136     122     116      93      75
                  74       9       6
 
NaeI GCC'GGC

Cuts at:      0      18     109    1818 
   Size:         18      91    1709

  Fragments arranged by size: 

                1709      91      18
 
NcoI C'CATG_G

Cuts at:      0    1733    1818 
   Size:       1733      85
 
NgoMIV G'CCGG_C

Cuts at:      0      16     107    1818 
   Size:         16      91    1711

  Fragments arranged by size: 

                1711      91      16
 
NsiI A_TGCA'T

Cuts at:      0     977    1818 
   Size:        977     841
 
NspI r_CATG'y

Cuts at:      0      81     891     975    1122    1818 
   Size:         81     810      84     147     696

  Fragments arranged by size: 

                 810     696     147      84      81
 
PasI CC'CwG_GG

Cuts at:      0     846    1473    1482    1636    1818 
   Size:        846     627       9     154     182

  Fragments arranged by size: 

                 846     627     182     154       9
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     321     510     552     912    1818 
   Size:        321     189      42     360     906

  Fragments arranged by size: 

                 906     360     321     189      42
 
PfoI T'CCnGG_A

Cuts at:      0     950    1238    1383    1818 
   Size:        950     288     145     435

  Fragments arranged by size: 

                 950     435     288     145
 
PmlI CAC'GTG

Cuts at:      0    1110    1818 
   Size:       1110     708
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     331     363    1818 
   Size:        331      32    1455

  Fragments arranged by size: 

                1455     331      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      54     621    1477    1486    1765    1818 
   Size:         54     567     856       9     279      53

  Fragments arranged by size: 

                 856     567     279      54      53       9
 
PspOMI G'GGCC_C

Cuts at:      0     115    1467    1530    1630    1648    1666    1818 
   Size:        115    1352      63     100      18      18     152

  Fragments arranged by size: 

                1352     152     115     100      63      18      18
 
PspXI vC'TCGA_Gb

Cuts at:      0    1544    1818 
   Size:       1544     274
 
PstI C_TGCA'G

Cuts at:      0     127     398     657     929    1818 
   Size:        127     271     259     272     889

  Fragments arranged by size: 

                 889     272     271     259     127
 
PvuII CAG'CTG

Cuts at:      0     372     447     598     924     930    1818 
   Size:        372      75     151     326       6     888

  Fragments arranged by size: 

                 888     372     326     151      75       6
 
SacI G_AGCT'C

Cuts at:      0    1187    1818 
   Size:       1187     631
 
SanDI GG'GwC_CC

Cuts at:      0    1477    1765    1818 
   Size:       1477     288      53

  Fragments arranged by size: 

                1477     288      53
 
SapI GCTCTTCn'nnn_

Cuts at:      0     565     787    1818 
   Size:        565     222    1031

  Fragments arranged by size: 

                1031     565     222
 
SexAI A'CCwGG_T

Cuts at:      0     536     896    1818 
   Size:        536     360     922

  Fragments arranged by size: 

                 922     536     360
 
SfcI C'TryA_G

Cuts at:      0     123     394     653     925    1818 
   Size:        123     271     259     272     893

  Fragments arranged by size: 

                 893     272     271     259     123
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1538    1638    1647    1674    1818 
   Size:       1538     100       9      27     144

  Fragments arranged by size: 

                1538     144     100      27       9
 
SmaI CCC'GGG

Cuts at:      0    1655    1781    1818 
   Size:       1655     126      37

  Fragments arranged by size: 

                1655     126      37
 
SmlI C'TyrA_G

Cuts at:      0    1123    1544    1818 
   Size:       1123     421     274

  Fragments arranged by size: 

                1123     421     274
 
SphI G_CATG'C

Cuts at:      0     891    1122    1818 
   Size:        891     231     696

  Fragments arranged by size: 

                 891     696     231
 
StuI AGG'CCT

Cuts at:      0    1559    1818 
   Size:       1559     259
 
StyI C'CwwG_G

Cuts at:      0    1733    1818 
   Size:       1733      85
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     112     643    1186    1818 
   Size:        112     531     543     632

  Fragments arranged by size: 

                 632     543     531     112
 
Tth111I GACn'n_nGTC

Cuts at:      0     270    1710    1818 
   Size:        270    1440     108

  Fragments arranged by size: 

                1440     270     108
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     600    1768    1818 
   Size:        600    1168      50

  Fragments arranged by size: 

                1168     600      50
 
XhoI C'TCGA_G

Cuts at:      0    1544    1818 
   Size:       1544     274
 
XmaI C'CCGG_G

Cuts at:      0    1653    1779    1818 
   Size:       1653     126      39

  Fragments arranged by size: 

                1653     126      39
 
XmnI GAAnn'nnTTC

Cuts at:      0    1343    1818 
   Size:       1343     475
 

 Enzymes that do cut:

     AcuI     AfeI     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BfrBI     BglI
 Bme1580I    BmgBI     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I
    BspEI    BspMI    BsrDI    BsrFI    BssSI   BstAPI   BstEII    BstXI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DrdI     EaeI     EagI
     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FseI
     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     MslI   MspA1I
     NaeI     NcoI   NgoMIV     NsiI     NspI     PasI    PflMI     PfoI
     PmlI     PpiI    PpuMI   PspOMI    PspXI     PstI    PvuII     SacI
    SanDI     SapI    SexAI     SfcI     SfiI     SmaI     SmlI     SphI
     StuI     StyI    TaqII  Tth111I     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI     AlfI     AscI     AseI    AsiSI    AvrII     BaeI     BbeI
    BbvCI    BciVI     BclI    BglII     BlpI     BmtI   Bpu10I    BsaBI
    BsaHI    BsiWI     BsmI    BsmBI    BspHI    BsrBI    BsrGI   BssHII
    BstBI  BstZ17I     ClaI    CspCI     DraI   DraIII     EciI    EcoRV
    FspAI     KasI     KpnI     MfeI     MluI     MmeI     MscI     NarI
     NdeI     NheI     NotI     NruI     PacI     PciI     PmeI    PshAI
     PsiI     PsrI     PvuI    RsrII    SacII     SalI     SbfI     ScaI
     SfoI     SgfI    SgrAI    SnaBI     SpeI     SrfI     SspI     SwaI
     TatI     TstI     XbaI     ZraI
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