Restriction map for
pF1KE1092
(Circular) (Six-base) MAPSORT of: pF1KE1092.seq Check: 9195 from: 1 to: 7757
pF1KE1092 7757 bp
With 182 enzymes: SgfI *
October 26, 2009 14:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1245 1816 1245
Size: 571 7186
AccI GT'mk_AC
Cuts at: 1186 1902 4795 1186
Size: 716 2893 4148
Fragments arranged by size:
4148 2893 716
Acc65I G'GTAC_C
Cuts at: 4773 4773
Size: 7757
AclI AA'CG_TT
Cuts at: 7597 7597
Size: 7757
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 242 1082 1600 1961 2705 3940 4369 4745 5582
Size: 840 518 361 744 1235 429 376 837
Cuts at: 5582 6014 6761 7029 242
Size: 432 747 268 970
Fragments arranged by size:
1235 970 840 837 747 744 518 432
429 376 361 268
AfeI AGC'GCT
Cuts at: 4597 4597
Size: 7757
AflIII A'CryG_T
Cuts at: 3728 4172 4672 5109 6213 7190 3728
Size: 444 500 437 1104 977 4295
Fragments arranged by size:
4295 1104 977 500 444 437
AgeI A'CCGG_T
Cuts at: 6145 6145
Size: 7757
AhdI GACnn_n'nnGTC
Cuts at: 261 3933 261
Size: 3672 4085
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1214 1246 3116 3148 1214
Size: 32 1870 32 5823
Fragments arranged by size:
5823 1870 32 32
AlwNI CAG_nnn'CTG
Cuts at: 292 2347 3519 4059 5062 6629 292
Size: 2055 1172 540 1003 1567 1420
Fragments arranged by size:
2055 1567 1420 1172 1003 540
ApaLI G'TGCA_C
Cuts at: 6527 6527
Size: 7757
ApoI r'AATT_y
Cuts at: 297 350 494 828 1944 1971 2814 3125 4046
Size: 53 144 334 1116 27 843 311 921
Cuts at: 4046 4761 7110 7366 297
Size: 715 2349 256 688
Fragments arranged by size:
2349 1116 921 843 715 688 334 311
256 144 53 27
AseI AT'TA_AT
Cuts at: 20 20
Size: 7757
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7757
AvaI C'yCGr_G
Cuts at: 4777 4777
Size: 7757
BamHI G'GATC_C
Cuts at: 4782 4782
Size: 7757
BanI G'GyrC_C
Cuts at: 894 3051 4773 5187 5435 5470 894
Size: 2157 1722 414 248 35 3181
Fragments arranged by size:
3181 2157 1722 414 248 35
BanII G_rGCy'C
Cuts at: 3668 4100 4771 5801 3668
Size: 432 671 1030 5624
Fragments arranged by size:
5624 1030 671 432
BbeI G_GCGC'C
Cuts at: 5191 5439 5191
Size: 248 7509
BbsI GAAGACnn'nnnn_
Cuts at: 1768 3026 3209 3295 3932 4733 1768
Size: 1258 183 86 637 801 4792
Fragments arranged by size:
4792 1258 801 637 183 86
BbvCI CC'TCA_GC
Cuts at: 1725 1860 1725
Size: 135 7622
BciVI GTATCCnnnnn_n'
Cuts at: 201 5655 6416 7757 201
Size: 5454 761 1341 201
Fragments arranged by size:
5454 1341 761 201
BclI T'GATC_A
Cuts at: 534 1313 5277 534
Size: 779 3964 3014
Fragments arranged by size:
3964 3014 779
BfrBI ATG'CAT
Cuts at: 2513 2513
Size: 7757
BglII A'GATC_T
Cuts at: 5272 7309 5272
Size: 2037 5720
BlpI GC'TnA_GC
Cuts at: 2801 4869 2801
Size: 2068 5689
Bme1580I G_kGCm'C
Cuts at: 1323 1988 2793 5382 5475 6531 1323
Size: 665 805 2589 93 1056 2549
Fragments arranged by size:
2589 2549 1056 805 665 93
BmrI ACTGGGnnnn_n'
Cuts at: 832 1317 2115 3765 4507 4969 5050 5385 7529
Size: 485 798 1650 742 462 81 335 2144
Cuts at: 7529 832
Size: 1060
Fragments arranged by size:
2144 1650 1060 798 742 485 462 335
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6124 6124
Size: 7757
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1135 2089 2741 3095 1135
Size: 954 652 354 5797
Fragments arranged by size:
5797 954 652 354
Bpu10I CC'TnA_GC
Cuts at: 1725 1860 4592 1725
Size: 135 2732 4890
Fragments arranged by size:
4890 2732 135
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 929 962 3271 3842 3860 4933 5812 6304 6602
Size: 33 2309 571 18 1073 879 492 298
Cuts at: 6602 929
Size: 2084
Fragments arranged by size:
2309 2084 1073 879 571 492 298 33
18
BsaI GGTCTCn'nnnn_
Cuts at: 620 2107 3987 7461 620
Size: 1487 1880 3474 916
Fragments arranged by size:
3474 1880 1487 916
BsaAI yAC'GTr
Cuts at: 4675 5110 4675
Size: 435 7322
BsaBI GATnn'nnATC
Cuts at: 6 5271 7308 6
Size: 5265 2037 455
Fragments arranged by size:
5265 2037 455
BsaHI Gr'CG_yC
Cuts at: 1069 5188 5436 6138 7634 1069
Size: 4119 248 702 1496 1192
Fragments arranged by size:
4119 1496 1192 702 248
BsaWI w'CCGG_w
Cuts at: 4946 5467 6145 6419 6566 6977 4946
Size: 521 678 274 147 411 5726
Fragments arranged by size:
5726 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2471 2501 2471
Size: 30 7727
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1244 2060 1244
Size: 816 6941
BseYI C'CCAG_C
Cuts at: 2126 2408 3419 4218 5182 5940 6517 7013 2126
Size: 282 1011 799 964 758 577 496 2870
Fragments arranged by size:
2870 1011 964 799 758 577 496 282
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 185 2451 2858 3924 185
Size: 2266 407 1066 4018
Fragments arranged by size:
4018 2266 1066 407
BsiEI CG_ry'CG
Cuts at: 104 5299 5345 6553 104
Size: 5195 46 1208 1308
Fragments arranged by size:
5195 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 4304 4771 5549 5739 6531 4304
Size: 467 778 190 792 5530
Fragments arranged by size:
5530 792 778 467 190
BsmI GAATG_Cn'
Cuts at: 278 1461 2515 3013 278
Size: 1183 1054 498 5022
Fragments arranged by size:
5022 1183 1054 498
BsmBI CGTCTCn'nnnn_
Cuts at: 2116 4326 2116
Size: 2210 5547
Bsp1286I G_dGCh'C
Cuts at: 1323 1988 2793 3668 4100 4304 4771 5382 5475
Size: 665 805 875 432 204 467 611 93
Cuts at: 5475 5549 5739 5801 6531 1323
Size: 74 190 62 730 2549
Fragments arranged by size:
2549 875 805 730 665 611 467 432
204 190 93 74 62
BspEI T'CCGG_A
Cuts at: 6977 6977
Size: 7757
BspHI T'CATG_A
Cuts at: 6933 6933
Size: 7757
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1245 1816 2636 4807 5021 5323 5704 1245
Size: 571 820 2171 214 302 381 3298
Fragments arranged by size:
3298 2171 820 571 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3908 6049 6103 7587 7752 50
Size: 3858 2141 54 1484 165 55
Fragments arranged by size:
3858 2141 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 301 896 2294 5669 301
Size: 595 1398 3375 2389
Fragments arranged by size:
3375 2389 1398 595
BsrFI r'CCGG_y
Cuts at: 5755 5936 6145 5755
Size: 181 209 7367
Fragments arranged by size:
7367 209 181
BsrGI T'GTAC_A
Cuts at: 1830 1830
Size: 7757
BssSI C'ACGA_G
Cuts at: 6028 6386 6028
Size: 358 7399
BstAPI GCAn_nnn'nTGC
Cuts at: 1913 5134 1913
Size: 3221 4536
BstBI TT'CG_AA
Cuts at: 6118 6118
Size: 7757
BstXI CCAn_nnnn'nTGG
Cuts at: 1755 2134 3419 1755
Size: 379 1285 6093
Fragments arranged by size:
6093 1285 379
BstYI r'GATC_y
Cuts at: 1 289 339 1720 3792 4782 5249 5272 5607
Size: 288 50 1381 2072 990 467 23 335
Cuts at: 5607 5853 6865 6951 6963 7309 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
2072 1381 1012 990 467 449 346 335
288 246 86 50 23 12
BstZ17I GTA'TAC
Cuts at: 1903 1903
Size: 7757
Bsu36I CC'TnA_GG
Cuts at: 3846 3846
Size: 7757
BtgI C'CryG_G
Cuts at: 107 2705 107
Size: 2598 5159
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5707 5888 6051 5707
Size: 181 163 7413
Fragments arranged by size:
7413 181 163
BtsI GCAGTG_nn'
Cuts at: 1515 5000 1515
Size: 3485 4272
DraI TTT'AAA
Cuts at: 552 1298 4247 4756 552
Size: 746 2949 509 3553
Fragments arranged by size:
3553 2949 746 509
DraIII CAC_nnn'GTG
Cuts at: 1211 1699 2149 1211
Size: 488 450 6819
Fragments arranged by size:
6819 488 450
DrdI GACnn_nn'nnGTC
Cuts at: 5463 6321 5463
Size: 858 6899
EaeI y'GGCC_r
Cuts at: 960 2546 5342 5516 5907 5934 7279 960
Size: 1586 2796 174 391 27 1345 1438
Fragments arranged by size:
2796 1586 1438 1345 391 174 27
EagI C'GGCC_G
Cuts at: 5342 5342
Size: 7757
EarI CTCTTCn'nnn_
Cuts at: 447 2486 3092 3275 3422 3627 3776 4187 5780
Size: 2039 606 183 147 205 149 411 1593
Cuts at: 5780 5990 447
Size: 210 2214
Fragments arranged by size:
2214 2039 1593 606 411 210 205 183
149 147
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 5965 6275 6421 7567 127
Size: 5838 310 146 1146 317
Fragments arranged by size:
5838 1146 317 310 146
EcoICRI GAG'CTC
Cuts at: 4769 4769
Size: 7757
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 242 1082 1135 1600 1961 2089 2705 2741 3095
Size: 840 53 465 361 128 616 36 354
Cuts at: 3095 3940 4369 4745 5582 6014 6761 7029 242
Size: 845 429 376 837 432 747 268 970
Fragments arranged by size:
970 845 840 837 747 616 465 432
429 376 361 354 268 128 53 36
EcoNI CCTnn'n_nnAGG
Cuts at: 1153 1153
Size: 7757
EcoO109I rG'GnC_Cy
Cuts at: 2728 3516 4896 2728
Size: 788 1380 5589
Fragments arranged by size:
5589 1380 788
EcoRI G'AATT_C
Cuts at: 350 828 1971 2814 3125 4046 4761 350
Size: 478 1143 843 311 921 715 3346
Fragments arranged by size:
3346 1143 921 843 715 478 311
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3235 4332 5776 3235
Size: 1097 1444 5216
Fragments arranged by size:
5216 1444 1097
FspI TGC'GCA
Cuts at: 5538 5538
Size: 7757
HaeII r_GCGC'y
Cuts at: 4226 4599 5191 5439 6461 7440 4226
Size: 373 592 248 1022 979 4543
Fragments arranged by size:
4543 1022 979 592 373 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 112 144 247 279 1615 1647 3282 3314 3376
Size: 32 103 32 1336 32 1635 32 62
Cuts at: 3376 3388 3408 3420 3520 3552 3557 3589 3919
Size: 12 20 12 100 32 5 32 330
Cuts at: 3919 3951 4329 4356 4361 4388 4499 4531 5599
Size: 32 378 27 5 27 111 32 1068
Cuts at: 5599 5629 5631 5661 112
Size: 30 2 30 2208
Fragments arranged by size:
2208 1635 1336 1068 378 330 111 103
100 62 32 32 32 32 32 32
32 32 30 30 27 27 20 12
12 5 5 2
HincII GTy'rAC
Cuts at: 1043 1376 2170 4670 4796 1043
Size: 333 794 2500 126 4004
Fragments arranged by size:
4004 2500 794 333 126
HindIII A'AGCT_T
Cuts at: 2939 3217 4309 2939
Size: 278 1092 6387
Fragments arranged by size:
6387 1092 278
HpaI GTT'AAC
Cuts at: 4670 4670
Size: 7757
KasI G'GCGC_C
Cuts at: 5187 5435 5187
Size: 248 7509
KpnI G_GTAC'C
Cuts at: 4777 4777
Size: 7757
MluI A'CGCG_T
Cuts at: 7190 7190
Size: 7757
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 635 2869 4305 4542 6428 6612 635
Size: 2234 1436 237 1886 184 1780
Fragments arranged by size:
2234 1886 1780 1436 237 184
MscI TGG'CCA
Cuts at: 962 2548 5518 962
Size: 1586 2970 3201
Fragments arranged by size:
3201 2970 1586
MslI CAynn'nnrTG
Cuts at: 428 3608 4506 5746 5873 428
Size: 3180 898 1240 127 2312
Fragments arranged by size:
3180 2312 1240 898 127
MspA1I CmG'CkG
Cuts at: 1114 1925 2098 4218 4868 5182 5542 6555 6800
Size: 811 173 2120 650 314 360 1013 245
Cuts at: 6800 1114
Size: 2071
Fragments arranged by size:
2120 2071 1013 811 650 360 314 245
173
NaeI GCC'GGC
Cuts at: 5938 5938
Size: 7757
NarI GG'CG_CC
Cuts at: 5188 5436 5188
Size: 248 7509
NcoI C'CATG_G
Cuts at: 107 2705 107
Size: 2598 5159
NdeI CA'TA_TG
Cuts at: 1380 1380
Size: 7757
NgoMIV G'CCGG_C
Cuts at: 5936 5936
Size: 7757
NsiI A_TGCA'T
Cuts at: 2515 2515
Size: 7757
NspI r_CATG'y
Cuts at: 264 1446 1614 2134 2517 3732 4176 4810 5310
Size: 1182 168 520 383 1215 444 634 500
Cuts at: 5310 6217 264
Size: 907 1804
Fragments arranged by size:
1804 1215 1182 907 634 520 500 444
383 168
PciI A'CATG_T
Cuts at: 3728 4172 6213 3728
Size: 444 2041 5272
Fragments arranged by size:
5272 2041 444
PflMI CCAn_nnn'nTGG
Cuts at: 361 1491 361
Size: 1130 6627
PfoI T'CCnGG_A
Cuts at: 3709 3709
Size: 7757
PmeI GTTT'AAAC
Cuts at: 4756 4756
Size: 7757
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3314 3346 6896 6928 3314
Size: 32 3550 32 4143
Fragments arranged by size:
4143 3550 32 32
PpuMI rG'GwC_Cy
Cuts at: 2728 3516 2728
Size: 788 6969
PsiI TTA'TAA
Cuts at: 2387 2387
Size: 7757
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4139 4171 4139
Size: 32 7725
PstI C_TGCA'G
Cuts at: 1827 1918 2097 4804 5489 1827
Size: 91 179 2707 685 4095
Fragments arranged by size:
4095 2707 685 179 91
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7757
PvuII CAG'CTG
Cuts at: 1114 1925 2098 4218 5182 5542 1114
Size: 811 173 2120 964 360 3329
Fragments arranged by size:
3329 2120 964 811 360 173
RsrII CG'GwC_CG
Cuts at: 5952 5952
Size: 7757
SacI G_AGCT'C
Cuts at: 4771 4771
Size: 7757
SalI G'TCGA_C
Cuts at: 4794 4794
Size: 7757
SapI GCTCTTCn'nnn_
Cuts at: 3422 3776 4187 5780 5990 3422
Size: 354 411 1593 210 5189
Fragments arranged by size:
5189 1593 411 354 210
SbfI CC_TGCA'GG
Cuts at: 4804 4804
Size: 7757
ScaI AGT'ACT
Cuts at: 2880 3855 7274 2880
Size: 975 3419 3363
Fragments arranged by size:
3419 3363 975
SfcI C'TryA_G
Cuts at: 33 1823 1914 1920 2093 2361 2807 3650 3897
Size: 1790 91 6 173 268 446 843 247
Cuts at: 3897 4800 5485 6478 6669 33
Size: 903 685 993 191 1121
Fragments arranged by size:
1790 1121 993 903 843 685 446 268
247 191 173 91 6
SfoI GGC'GCC
Cuts at: 5189 5437 5189
Size: 248 7509
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7757
SmaI CCC'GGG
Cuts at: 4779 4779
Size: 7757
SmlI C'TyrA_G
Cuts at: 944 977 3250 3857 3875 4912 5827 6319 6581
Size: 33 2273 607 18 1037 915 492 262
Cuts at: 6581 944
Size: 2120
Fragments arranged by size:
2273 2120 1037 915 607 492 262 33
18
SpeI A'CTAG_T
Cuts at: 67 1342 67
Size: 1275 6482
SphI G_CATG'C
Cuts at: 4810 5310 4810
Size: 500 7257
SspI AAT'ATT
Cuts at: 846 2299 2700 846
Size: 1453 401 5903
Fragments arranged by size:
5903 1453 401
StuI AGG'CCT
Cuts at: 3820 3820
Size: 7757
StyI C'CwwG_G
Cuts at: 107 2705 2920 3363 3776 4041 4891 5245 107
Size: 2598 215 443 413 265 850 354 2619
Fragments arranged by size:
2619 2598 850 443 413 354 265 215
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 5340 5473 5932 6141 5340
Size: 133 459 209 6956
Fragments arranged by size:
6956 459 209 133
TatI w'GTAC_w
Cuts at: 1830 2878 3370 3853 4352 7272 1830
Size: 1048 492 483 499 2920 2315
Fragments arranged by size:
2920 2315 1048 499 492 483
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2471 2503 4223 4255 7274 7306 2471
Size: 32 1720 32 3019 32 2922
Fragments arranged by size:
3019 2922 1720 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 5554 5554
Size: 7757
XbaI T'CTAG_A
Cuts at: 3336 4788 3336
Size: 1452 6305
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5252 5252
Size: 7757
XmaI C'CCGG_G
Cuts at: 4777 4777
Size: 7757
XmnI GAAnn'nnTTC
Cuts at: 1948 1971 2617 2748 3218 3332 3490 3929 1948
Size: 23 646 131 470 114 158 439 5776
Fragments arranged by size:
5776 646 470 439 158 131 114 23
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI
NspI PciI PflMI PfoI PmeI PpiI PpuMI PsiI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AleI AlfI ApaI AscI AvrII BaeI
BcgI BglI BmgBI BmtI BsiWI BssHII BstEII ClaI
CspCI EcoRV FseI FspAI MfeI NheI NotI NruI
PacI PasI PmlI PshAI PspOMI PspXI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI