Restriction map for pF1KE1056
##################################
# NO CUT ENZYMES (   99 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcyI        AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaLI       AscI        Asp718I     
AspEI       AspI        AviII       AvrII       BamHI       
BanI        BbeI        BbrPI       BcgI        BciVI       
BlnI        BlpI        Bpu1102I    BsaBI       BsaHI       
BsiEI       BsiWI       BsmBI       Bsp1407I    BspDI       
BspLU11I    BsrBI       BsrFI       BsrGI       BssHII      
Bst1107I    BstUI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoRI       EcoRV       FseI        FspI        
Hin1I       KasI        KpnI        KspI        MamI        
MluI        MvnI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        PacI        
PflFI       PinAI       PmaCI       PmeI        PmlI        
Psp1406I    PvuI        RsrII       SacII       SalI        
SbfI        SexAI       SfiI        SfoI        SgrAI       
SmaI        SnaBI       SphI        Sse8387I    SspBI       
StuI        Tth111I     XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AccIII        778  TCCGGA               T^CCGGA
AflII        1921  CTTAAG               C^TTAAG
AlwNI        3425  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          406  GGGCCC               GGGCC^C
BbsI         1726  GAAGAC               GAAGAC(2/6)
BbvCI        3422  CCTCAGC              CCTCAGC(-5/-2)
BcnI         3309  CCGGG                CC^[GC]GG
BfrI         1921  CTTAAG               C^TTAAG
BglI         3316  GCCATGGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        3410  AGATCT               A^GATCT
BpuAI        1726  GAAGAC               GAAGAC(2/6)
BsaI         3304  GGTCTC               GGTCTC(1/5)
BsaWI         778  TCCGGA               [AT]^CCGG[AT]
BseAI         778  TCCGGA               T^CCGGA
BspEI         778  TCCGGA               T^CCGGA
BssSI         683  CACGAG               CACGAG(-5/-1)
BstEII        995  GGTCACC              G^GT[ACGT]ACC
BstPI         995  GGTCACC              G^GT[ACGT]ACC
BstXI        3225  CCAATCTGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI          501  TTTAAA               TTT^AAA
EcoNI        3709  CCTAGTGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       995  GGTCACC              G^GT[ACGT]ACC
EcoT22I      2519  ATGCAT               ATGCA^T
FauI          897  CCCGC                CCCGC(4/6)
HaeII        3604  AGCGCC               [GA]GCGC^[CT]
HgaI         2424  GCGTC                GACGC(5/10)
HindIII       770  AAGCTT               A^AGCTT
HpaI          196  GTTAAC               GTT^AAC
MroI          778  TCCGGA               T^CCGGA
NciI         3309  CCGGG                CC^[GC]GG
NsiI         2519  ATGCAT               ATGCA^T
PshAI        3177  GACTTTAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI         3193  CTGCAG               CTGCA^G
SacI         2336  GAGCTC               GAGCT^C
SapI         1387  GAAGAGC              GCTCTTC(1/4)
ScaI         1575  AGTACT               AGT^ACT
SpeI         1027  ACTAGT               A^CTAGT
SwaI          500  ATTTAAAT             ATTT^AAAT


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AseI         1552  ATTAAT               AT^TAAT
             2893  ATTAAT              
AsnI         1552  ATTAAT               AT^TAAT
             2893  ATTAAT              
BalI         2439  TGGCCA               TGG^CCA
             2450  TGGCCA              
BanII         406  GGGCCC               G[GA]GC[CT]^C
             2336  GAGCTC              
BclI          615  TGATCA               T^GATCA
             2739  TGATCA              
BsaAI        2623  TACGTG               [CT]AC^GT[GA]
             2743  CACGTA              
BsgI         2328  GTGCAG               GTGCAG(16/14)
             3632  GTGCAG              
BsmFI         416  GGGAC                GGGAC(10/14)
             2553  GGGAC               
BspHI        1125  TCATGA               T^CATGA
             3279  TCATGA              
CfoI         2085  GCGC                 GCG^C
             3605  GCGC                
DsaI          323  CCATGG               C^C[GA][CT]GG
             3317  CCATGG              
FbaI          615  TGATCA               T^GATCA
             2739  TGATCA              
HapII         779  CCGG                 C^CGG
             3309  CCGG                
HhaI         2085  GCGC                 GCG^C
             3605  GCGC                
HinP1I       2085  GCGC                 G^CGC
             3605  GCGC                
HpaII         779  CCGG                 C^CGG
             3309  CCGG                
MluNI        2439  TGGCCA               TGG^CCA
             2450  TGGCCA              
MscI         2439  TGGCCA               TGG^CCA
             2450  TGGCCA              
MspI          779  CCGG                 C^CGG
             3309  CCGG                
NcoI          323  CCATGG               C^CATGG
             3317  CCATGG              
NspI         1646  GCATGT               [GA]CATG^[CT]
             1868  GCATGT              
PaeR7I       2072  CTCGAG               C^TCGAG
             3152  CTCGAG              
PpuMI         330  AGGACCT              [GA]G^G[AT]CC[CT]
              609  GGGTCCT             
PshBI        1552  ATTAAT               AT^TAAT
             2893  ATTAAT              
RcaI         1125  TCATGA               T^CATGA
             3279  TCATGA              
XhoI         2072  CTCGAG               C^TCGAG
             3152  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (   95 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        Asp700I     AspHI       AvaI        AvaII       
BbvI        BfaI        BmyI        BpmI        BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmI        BsoBI       Bsp1286I    BspMI       BsrDI       
BsrI        BssKI       BstAPI      BstBI       BstF5I      
BstNI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       DraII       EaeI        EarI        EcoO109I    
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HincII      HindII      HinfI       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MfeI        MflI        MnlI        MseI        
MslI        MspA1I      MunI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       NspV        PflMI       
PleI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SfuI        SmlI        
SspI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XbaI        XcmI        XhoII       XmnI        
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