Restriction map for pF1KE1056
 (Linear) (Six-base) MAPSORT of: KIEE1056.seq  Check: 3064  from: 1  to: 3810



KIEE1056.orf

 With 182 enzymes: SgfI * 

                        February 1, 2012 16:00  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    3625    3810 
   Size:       3625     185
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     323     923    1625    3065    3810 
   Size:        323     600     702    1440     745

  Fragments arranged by size: 

                1440     745     702     600     323
 
AflII C'TTAA_G

Cuts at:      0    1921    3810 
   Size:       1921    1889
 
AleI CACnn'nnGTG

Cuts at:      0     604    3810 
   Size:        604    3206
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2417    3810 
   Size:       2417    1393
 
AlwNI CAG_nnn'CTG

Cuts at:      0    3430    3810 
   Size:       3430     380
 
ApaI G_GGCC'C

Cuts at:      0     410    3810 
   Size:        410    3400
 
ApoI r'AATT_y

Cuts at:      0      15      97     147    1174    1209    2715    3810 
   Size:         15      82      50    1027      35    1506    1095

  Fragments arranged by size: 

                1506    1095    1027      82      50      35      15
 
AseI AT'TA_AT

Cuts at:      0    1553    2894    3810 
   Size:       1553    1341     916

  Fragments arranged by size: 

                1553    1341     916
 
AvaI C'yCGr_G

Cuts at:      0    2072    3152    3665    3810 
   Size:       2072    1080     513     145

  Fragments arranged by size: 

                2072    1080     513     145
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     733     766    3810 
   Size:        733      33    3044

  Fragments arranged by size: 

                3044     733      33
 
BanII G_rGCy'C

Cuts at:      0     410    2340    3810 
   Size:        410    1930    1470

  Fragments arranged by size: 

                1930    1470     410
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1733    3810 
   Size:       1733    2077
 
BbvCI CC'TCA_GC

Cuts at:      0    3423    3810 
   Size:       3423     387
 
BclI T'GATC_A

Cuts at:      0     615    2739    3810 
   Size:        615    2124    1071

  Fragments arranged by size: 

                2124    1071     615
 
BfrBI ATG'CAT

Cuts at:      0    2521    3810 
   Size:       2521    1289
 
BglI GCCn_nnn'nGGC

Cuts at:      0    3322    3810 
   Size:       3322     488
 
BglII A'GATC_T

Cuts at:      0    3410    3810 
   Size:       3410     400
 
Bme1580I G_kGCm'C

Cuts at:      0     410     729    3810 
   Size:        410     319    3081

  Fragments arranged by size: 

                3081     410     319
 
BmgBI CAC'GTC

Cuts at:      0    3478    3810 
   Size:       3478     332
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1260    1699    3810 
   Size:       1260     439    2111

  Fragments arranged by size: 

                2111    1260     439
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1889    3810 
   Size:       1889    1921
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1052    1970    3710    3810 
   Size:       1052     918    1740     100

  Fragments arranged by size: 

                1740    1052     918     100
 
Bpu10I CC'TnA_GC

Cuts at:      0    3423    3810 
   Size:       3423     387
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     432     821     904    1075    2293    3810 
   Size:        432     389      83     171    1218    1517

  Fragments arranged by size: 

                1517    1218     432     389     171      83
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    3310    3810 
   Size:       3310     500
 
BsaAI yAC'GTr

Cuts at:      0    2625    2745    3810 
   Size:       2625     120    1065

  Fragments arranged by size: 

                2625    1065     120
 
BsaWI w'CCGG_w

Cuts at:      0     778    3810 
   Size:        778    3032
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     264     294     794     824    1141    1171    3810 
   Size:        264      30     500      30     317      30    2639

  Fragments arranged by size: 

                2639     500     317     264      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     437    1397    3671    3707    3761    3809    3810 
   Size:        437     960    2274      36      54      48       1

  Fragments arranged by size: 

                2274     960     437      54      48      36       1
 
BseYI C'CCAG_C

Cuts at:      0     508     663    2697    3810 
   Size:        508     155    2034    1113

  Fragments arranged by size: 

                2034    1113     508     155
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2349    3653    3810 
   Size:       2349    1304     157

  Fragments arranged by size: 

                2349    1304     157
 
BsiHKAI G_wGCw'C

Cuts at:      0    1481    2340    2825    3810 
   Size:       1481     859     485     985

  Fragments arranged by size: 

                1481     985     859     485
 
BsmI GAATG_Cn'

Cuts at:      0    1502    1733    2058    3810 
   Size:       1502     231     325    1752

  Fragments arranged by size: 

                1752    1502     325     231
 
Bsp1286I G_dGCh'C

Cuts at:      0     410     729    1481    2340    2825    3810 
   Size:        410     319     752     859     485     985

  Fragments arranged by size: 

                 985     859     752     485     410     319
 
BspEI T'CCGG_A

Cuts at:      0     778    3810 
   Size:        778    3032
 
BspHI T'CATG_A

Cuts at:      0    1125    3279    3810 
   Size:       1125    2154     531

  Fragments arranged by size: 

                2154    1125     531
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2668    3360    3625    3810 
   Size:       2668     692     265     185

  Fragments arranged by size: 

                2668     692     265     185
 
BsrDI GCAATG_nn'

Cuts at:      0     183    2389    2645    3810 
   Size:        183    2206     256    1165

  Fragments arranged by size: 

                2206    1165     256     183
 
BssSI C'ACGA_G

Cuts at:      0     683    3810 
   Size:        683    3127
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     855    2527    3243    3810 
   Size:        855    1672     716     567

  Fragments arranged by size: 

                1672     855     716     567
 
BstBI TT'CG_AA

Cuts at:      0     210     953    3147    3810 
   Size:        210     743    2194     663

  Fragments arranged by size: 

                2194     743     663     210
 
BstEII G'GTnAC_C

Cuts at:      0     995    3810 
   Size:        995    2815
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    3232    3810 
   Size:       3232     578
 
BstYI r'GATC_y

Cuts at:      0     578     906    3410    3727    3810 
   Size:        578     328    2504     317      83

  Fragments arranged by size: 

                2504     578     328     317      83
 
BtgI C'CryG_G

Cuts at:      0     323    3317    3810 
   Size:        323    2994     493

  Fragments arranged by size: 

                2994     493     323
 
BtsI GCAGTG_nn'

Cuts at:      0      80    2216    3576    3810 
   Size:         80    2136    1360     234

  Fragments arranged by size: 

                2136    1360     234      80
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    2124    2159    3810 
   Size:       2124      35    1651

  Fragments arranged by size: 

                2124    1651      35
 
DraI TTT'AAA

Cuts at:      0     503    3810 
   Size:        503    3307
 
EaeI y'GGCC_r

Cuts at:      0    2295    2439    2450    3810 
   Size:       2295     144      11    1360

  Fragments arranged by size: 

                2295    1360     144      11
 
EarI CTCTTCn'nnn_

Cuts at:      0     341    1382    2218    3786    3810 
   Size:        341    1041     836    1568      24

  Fragments arranged by size: 

                1568    1041     836     341      24
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     251    3810 
   Size:        251    3559
 
EcoICRI GAG'CTC

Cuts at:      0    2338    3810 
   Size:       2338    1472
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     323     923    1052    1625    1970    3065    3710    3810 
   Size:        323     600     129     573     345    1095     645     100

  Fragments arranged by size: 

                1095     645     600     573     345     323     129     100
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    3713    3810 
   Size:       3713      97
 
EcoO109I rG'GnC_Cy

Cuts at:      0     331     383     610    1048    3706    3761    3810 
   Size:        331      52     227     438    2658      55      49

  Fragments arranged by size: 

                2658     438     331     227      55      52      49
 
HaeII r_GCGC'y

Cuts at:      0    3608    3810 
   Size:       3608     202
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     430     462    2621    2653    3135    3167    3810 
   Size:        430      32    2159      32     482      32     643

  Fragments arranged by size: 

                2159     643     482     430      32      32      32
 
HincII GTy'rAC

Cuts at:      0     198    2543    2924    3810 
   Size:        198    2345     381     886

  Fragments arranged by size: 

                2345     886     381     198
 
HindIII A'AGCT_T

Cuts at:      0     770    3810 
   Size:        770    3040
 
HpaI GTT'AAC

Cuts at:      0     198    3810 
   Size:        198    3612
 
MfeI C'AATT_G

Cuts at:      0    1668    1876    2459    3810 
   Size:       1668     208     583    1351

  Fragments arranged by size: 

                1668    1351     583     208
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1034    1094    2091    3350    3810 
   Size:       1034      60     997    1259     460

  Fragments arranged by size: 

                1259    1034     997     460      60
 
MscI TGG'CCA

Cuts at:      0    2441    2452    3810 
   Size:       2441      11    1358

  Fragments arranged by size: 

                2441    1358      11
 
MslI CAynn'nnrTG

Cuts at:      0     139     604    1738    3810 
   Size:        139     465    1134    2072

  Fragments arranged by size: 

                2072    1134     465     139
 
MspA1I CmG'CkG

Cuts at:      0     663     855    3427    3810 
   Size:        663     192    2572     383

  Fragments arranged by size: 

                2572     663     383     192
 
NcoI C'CATG_G

Cuts at:      0     323    3317    3810 
   Size:        323    2994     493

  Fragments arranged by size: 

                2994     493     323
 
NsiI A_TGCA'T

Cuts at:      0    2523    3810 
   Size:       2523    1287
 
NspI r_CATG'y

Cuts at:      0    1650    1872    3810 
   Size:       1650     222    1938

  Fragments arranged by size: 

                1938    1650     222
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     247    2026    2448    3810 
   Size:        247    1779     422    1362

  Fragments arranged by size: 

                1779    1362     422     247
 
PfoI T'CCnGG_A

Cuts at:      0    1947    3810 
   Size:       1947    1863
 
PpuMI rG'GwC_Cy

Cuts at:      0     331     610    3810 
   Size:        331     279    3200

  Fragments arranged by size: 

                3200     331     279
 
PshAI GACnn'nnGTC

Cuts at:      0    3181    3810 
   Size:       3181     629
 
PsiI TTA'TAA

Cuts at:      0     128    3810 
   Size:        128    3682
 
PspOMI G'GGCC_C

Cuts at:      0     406    3810 
   Size:        406    3404
 
PspXI vC'TCGA_Gb

Cuts at:      0    3152    3810 
   Size:       3152     658
 
PstI C_TGCA'G

Cuts at:      0    3197    3810 
   Size:       3197     613
 
PvuII CAG'CTG

Cuts at:      0     663     855    3427    3810 
   Size:        663     192    2572     383

  Fragments arranged by size: 

                2572     663     383     192
 
SacI G_AGCT'C

Cuts at:      0    2340    3810 
   Size:       2340    1470
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1382    3810 
   Size:       1382    2428
 
ScaI AGT'ACT

Cuts at:      0    1577    3810 
   Size:       1577    2233
 
SfcI C'TryA_G

Cuts at:      0     290    1410    1469    3193    3564    3651    3699    3720 
   Size:        290    1120      59    1724     371      87      48      21

Cuts at:   3720    3810 
   Size:         90

  Fragments arranged by size: 

                1724    1120     371     290      90      87      59      48
                  21
 
SmlI C'TyrA_G

Cuts at:      0     447     836     883    1090    1921    2072    2272    3152 
   Size:        447     389      47     207     831     151     200     880

Cuts at:   3152    3810 
   Size:        658

  Fragments arranged by size: 

                 880     831     658     447     389     207     200     151
                  47
 
SpeI A'CTAG_T

Cuts at:      0    1027    3810 
   Size:       1027    2783
 
SspI AAT'ATT

Cuts at:      0     562    1660    1717    2142    3810 
   Size:        562    1098      57     425    1668

  Fragments arranged by size: 

                1668    1098     562     425      57
 
StyI C'CwwG_G

Cuts at:      0     323     530     582     991    1021    2120    3317    3810 
   Size:        323     207      52     409      30    1099    1197     493

  Fragments arranged by size: 

                1197    1099     493     409     323     207      52      30
            
 
SwaI ATTT'AAAT

Cuts at:      0     503    3810 
   Size:        503    3307
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     434     616     970    1313    2779    3810 
   Size:        434     182     354     343    1466    1031

  Fragments arranged by size: 

                1466    1031     434     354     343     182
 
TatI w'GTAC_w

Cuts at:      0     596    1465    1575    1925    3454    3810 
   Size:        596     869     110     350    1529     356

  Fragments arranged by size: 

                1529     869     596     356     350     110
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     794     826    1245    1277    3810 
   Size:        794      32     419      32    2533

  Fragments arranged by size: 

                2533     794     419      32      32
 
XbaI T'CTAG_A

Cuts at:      0     480    1275    3415    3810 
   Size:        480     795    2140     395

  Fragments arranged by size: 

                2140     795     480     395
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1010    3324    3748    3810 
   Size:       1010    2314     424      62

  Fragments arranged by size: 

                2314    1010     424      62
 
XhoI C'TCGA_G

Cuts at:      0    2072    3152    3810 
   Size:       2072    1080     658

  Fragments arranged by size: 

                2072    1080     658
 
XmnI GAAnn'nnTTC

Cuts at:      0     236    1718    2212    3143    3810 
   Size:        236    1482     494     931     667

  Fragments arranged by size: 

                1482     931     667     494     236
 

 Enzymes that do cut:

     AarI     AcuI    AflII     AleI     AlfI    AlwNI     ApaI     ApoI
     AseI     AvaI     BaeI    BanII     BbsI    BbvCI     BclI    BfrBI
     BglI    BglII Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaWI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrDI    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI     BtsI    CspCI
     DraI     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    HaeII    Hin4I   HincII  HindIII     HpaI     MfeI     MmeI     MscI
     MslI   MspA1I     NcoI     NsiI     NspI    PflMI     PfoI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PstI    PvuII     SacI     SapI
     ScaI     SfcI     SmlI     SpeI     SspI     StyI     SwaI    TaqII
     TatI     TstI     XbaI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI     AhdI
     AloI    ApaLI     AscI    AsiSI    AvrII    BamHI     BanI     BbeI
     BcgI    BciVI     BlpI     BmtI    BsaBI    BsaHI    BsiEI    BsiWI
    BsmBI    BsrBI    BsrFI    BsrGI   BssHII  BstZ17I   Bsu36I    BtgZI
     ClaI   DraIII     DrdI     EagI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI     KasI     KpnI     MluI     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     PacI     PasI     PciI     PmeI
     PmlI     PpiI     PsrI     PvuI    RsrII    SacII     SalI    SanDI
     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SphI     SrfI     StuI  Tth111I     XmaI     ZraI
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