Restriction map for pF1KB8659
##################################
# NO CUT ENZYMES (  106 enzymes) #
##################################

Acc65I      AccIII      AclI        AflII       AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AviII       AvrII       BalI        BamHI       
BbrPI       BcgI        BfrI        BglI        BglII       
BlnI        BsaAI       BsaWI       BseAI       BsgI        
BsiWI       BsmI        Bsp1407I    BspDI       BspEI       
BspMI       BsrBI       BsrDI       BsrGI       BssSI       
BstEII      BstPI       BstXI       Bsu36I      ClaI        
CpoI        DraI        DrdI        DsaI        Eam1105I    
Eco47III    Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FseI        FspI        HgaI        
HindIII     HpaI        KpnI        KspI        MluI        
MluNI       MroI        MscI        NaeI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        PacI        PaeR7I      PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshBI       
Psp1406I    PvuI        PvuII       RsrII       SacII       
SalI        SapI        SbfI        ScaI        SfiI        
SgrAI       SmlI        SnaBI       SphI        Sse8387I    
SspBI       StuI        SwaI        Van91I      XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AatII         419  GACGTC               GACGT^C
AccII        1595  CGCG                 CG^CG
AflIII       1440  ACATGT               A^C[GA][CT]GT
ApaI           41  GGGCCC               GGGCC^C
AspI         1612  GACTATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         1849  CCCGGG               C^[CT]CG[GA]G
BanI          491  GGCGCC               G^G[CT][GA]CC
BbeI          491  GGCGCC               GGCGC^C
BbvCI         253  GCTGAGG              CCTCAGC(-5/-2)
BlpI          655  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      655  GCTGAGC              GC^T[ACGT]AGC
BsaBI        1489  GATGAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          345  GAGACC               GGTCTC(1/5)
BsmBI         417  GAGACG               CGTCTC(1/5)
BsoBI        1849  CCCGGG               C^[CT]CG[GA]G
BspHI        1556  TCATGA               T^CATGA
BspLU11I     1440  ACATGT               A^CATGT
BsrFI          22  GCCGGT               [GA]^CCGG[CT]
BssHII       1594  GCGCGC               G^CGCGC
Bst1107I      335  GTATAC               GTA^TAC
BstBI         685  TTCGAA               TT^CGAA
BstUI        1595  CGCG                 CG^CG
BstZ17I       335  GTATAC               GTA^TAC
CelII         655  GCTGAGC              GC^T[ACGT]AGC
Cfr10I         22  GCCGGT               [GA]^CCGG[CT]
DraII          41  GGGCCCT              [GA]G^G[ACGT]CC[CT]
DraIII        878  CACTTAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI           20  CGGCCG               C^GGCCG
EclXI          20  CGGCCG               C^GGCCG
Eco52I         20  CGGCCG               C^GGCCG
EcoO109I       41  GGGCCCT              [GA]G^G[ACGT]CC[CT]
FauI         1897  GCGGG                CCCGC(4/6)
HaeII         491  GGCGCC               [GA]GCGC^[CT]
KasI          491  GGCGCC               G^GCGCC
MamI         1489  GATGAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         1379  CAATTG               C^AATTG
MunI         1379  CAATTG               C^AATTG
MvnI         1595  CGCG                 CG^CG
NarI          491  GGCGCC               GG^CGCC
NspV          685  TTCGAA               TT^CGAA
PflFI        1612  GACTATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI         269  GACTACTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI         1556  TCATGA               T^CATGA
SexAI        1251  ACCTGGT              A^CC[AT]GGT
SfoI          491  GGCGCC               GGC^GCC
SfuI          685  TTCGAA               TT^CGAA
SmaI         1849  CCCGGG               CCC^GGG
SpeI         1416  ACTAGT               A^CTAGT
Tth111I      1612  GACTATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI          408  TCTAGA               T^CTAGA
XcmI         1641  CCATCCTCCTCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI        1849  CCCGGG               C^CCGGG
XmaI         1849  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AcsI          477  AAATTT               [GA]^AATT[CT]
              830  AAATTT              
AcyI          419  GACGTC               G[GA]^CG[CT]C
              491  GGCGCC              
AfaI          681  GTAC                 GT^AC
             1635  GTAC                
AlwI         1495  GATCC                GGATC(4/5)
             1660  GATCC               
ApoI          477  AAATTT               [GA]^AATT[CT]
              830  AAATTT              
AvaII         531  GGTCC                G^G[AT]CC
              976  GGTCC               
BbsI          943  GAAGAC               GAAGAC(2/6)
              970  GAAGAC              
BciVI        1540  GGATAC               GTATCC(6/5)
             1090  GTATCC              
BclI          629  TGATCA               T^GATCA
             1383  TGATCA              
BpmI          501  CTGGAG               CTGGAG(16/14)
             1108  CTGGAG              
BpuAI         943  GAAGAC               GAAGAC(2/6)
              970  GAAGAC              
BsaHI         419  GACGTC               G[GA]^CG[CT]C
              491  GGCGCC              
BsiEI          20  CGGCCG               CG[GA][CT]^CG
             1163  CGGTCG              
BsmAI         345  GAGAC                GTCTC(1/5)
              417  GAGAC               
BsmFI          36  GGGAC                GGGAC(10/14)
              977  GTCCC               
BstAPI        196  GCAGAACTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1870  GCATCCACTGC         
BstYI        1494  AGATCC               [GA]^GATC[CT]
             1659  AGATCC              
EaeI           20  CGGCCG               [CT]^GGCC[GA]
              725  TGGCCG              
EcoT14I       980  CCAAGG               C^C[AT][AT]GG
             1346  CCAAGG              
FbaI          629  TGATCA               T^GATCA
             1383  TGATCA              
Hin1I         419  GACGTC               G[GA]^CG[CT]C
              491  GGCGCC              
HincII       1527  GTTGAC               GT[CT]^[GA]AC
             1774  GTTGAC              
HindII       1527  GTTGAC               GT[CT]^[GA]AC
             1774  GTTGAC              
MflI         1494  AGATCC               [GA]^GATC[CT]
             1659  AGATCC              
MslI          103  CACACAAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1212  CACCATCATG          
NspI         1440  ACATGT               [GA]CATG^[CT]
             1544  ACATGC              
RsaI          681  GTAC                 GT^AC
             1635  GTAC                
SspI          235  AATATT               AAT^ATT
             1429  AATATT              
StyI          980  CCAAGG               C^C[AT][AT]GG
             1346  CCAAGG              
XhoII        1494  AGATCC               [GA]^GATC[CT]
             1659  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   69 enzymes) #
#########################################

AccI        AciI        AluI        AlwNI       AspHI       
BanII       BbvI        BcnI        BfaI        BmyI        
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
EarI        EcoRII      Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MnlI        MseI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        PstI        
SacI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     
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