Restriction map for pF1KB8659
 (Linear) (Six-base) MAPSORT of: KIBB8659.seq  Check: 2009  from: 1  to: 1974



KIBB8659.orf

 With 182 enzymes: SgfI * 

                        July 28, 2009 13:24  ..

AatII G_ACGT'C

Cuts at:      0     423    1974 
   Size:        423    1551
 
AccI GT'mk_AC

Cuts at:      0     336    1342    1950    1974 
   Size:        336    1006     608      24

  Fragments arranged by size: 

                1006     608     336      24
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     133    1627    1974 
   Size:        133    1494     347

  Fragments arranged by size: 

                1494     347     133
 
AflIII A'CryG_T

Cuts at:      0    1440    1974 
   Size:       1440     534
 
AleI CACnn'nnGTG

Cuts at:      0     107    1974 
   Size:        107    1867
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     683    1974 
   Size:        683    1291
 
AlwNI CAG_nnn'CTG

Cuts at:      0     160     453    1030    1974 
   Size:        160     293     577     944

  Fragments arranged by size: 

                 944     577     293     160
 
ApaI G_GGCC'C

Cuts at:      0      45    1974 
   Size:         45    1929
 
ApoI r'AATT_y

Cuts at:      0     477     830    1974 
   Size:        477     353    1144

  Fragments arranged by size: 

                1144     477     353
 
AvaI C'yCGr_G

Cuts at:      0    1849    1974 
   Size:       1849     125
 
BanI G'GyrC_C

Cuts at:      0     491    1974 
   Size:        491    1483
 
BanII G_rGCy'C

Cuts at:      0      18      45     172    1115    1885    1974 
   Size:         18      27     127     943     770      89

  Fragments arranged by size: 

                 943     770     127      89      27      18
 
BbeI G_GCGC'C

Cuts at:      0     495    1974 
   Size:        495    1479
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     950     977    1974 
   Size:        950      27     997

  Fragments arranged by size: 

                 997     950      27
 
BbvCI CC'TCA_GC

Cuts at:      0     254    1974 
   Size:        254    1720
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1101    1534    1974 
   Size:       1101     433     440

  Fragments arranged by size: 

                1101     440     433
 
BclI T'GATC_A

Cuts at:      0     629    1383    1974 
   Size:        629     754     591

  Fragments arranged by size: 

                 754     629     591
 
BlpI GC'TnA_GC

Cuts at:      0     656    1974 
   Size:        656    1318
 
Bme1580I G_kGCm'C

Cuts at:      0      45    1974 
   Size:         45    1929
 
BmgBI CAC'GTC

Cuts at:      0     959    1974 
   Size:        959    1015
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1142    1974 
   Size:       1142     832
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     522    1129    1974 
   Size:        522     607     845

  Fragments arranged by size: 

                 845     607     522
 
Bpu10I CC'TnA_GC

Cuts at:      0     254    1974 
   Size:        254    1720
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     339    1974 
   Size:        339    1635
 
BsaBI GATnn'nnATC

Cuts at:      0    1493    1974 
   Size:       1493     481
 
BsaHI Gr'CG_yC

Cuts at:      0     420     492    1974 
   Size:        420      72    1482

  Fragments arranged by size: 

                1482     420      72
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      76     106    1974 
   Size:         76      30    1868

  Fragments arranged by size: 

                1868      76      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      24     298     357     949     952    1637    1974 
   Size:         24     274      59     592       3     685     337

  Fragments arranged by size: 

                 685     592     337     274      59      24       3
 
BseYI C'CCAG_C

Cuts at:      0      33     858    1974 
   Size:         33     825    1116

  Fragments arranged by size: 

                1116     825      33
 
BsiEI CG_ry'CG

Cuts at:      0      23    1166    1974 
   Size:         23    1143     808

  Fragments arranged by size: 

                1143     808      23
 
BsiHKAI G_wGCw'C

Cuts at:      0     172     964    1115    1885    1974 
   Size:        172     792     151     770      89

  Fragments arranged by size: 

                 792     770     172     151      89
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     411    1974 
   Size:        411    1563
 
Bsp1286I G_dGCh'C

Cuts at:      0      18      45     172     964    1115    1885    1974 
   Size:         18      27     127     792     151     770      89

  Fragments arranged by size: 

                 792     770     151     127      89      27      18
 
BspHI T'CATG_A

Cuts at:      0    1556    1974 
   Size:       1556     418
 
BsrFI r'CCGG_y

Cuts at:      0      22    1974 
   Size:         22    1952
 
BssHII G'CGCG_C

Cuts at:      0    1594    1974 
   Size:       1594     380
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     202    1876    1974 
   Size:        202    1674      98

  Fragments arranged by size: 

                1674     202      98
 
BstBI TT'CG_AA

Cuts at:      0     686    1974 
   Size:        686    1288
 
BstYI r'GATC_y

Cuts at:      0    1494    1659    1974 
   Size:       1494     165     315

  Fragments arranged by size: 

                1494     315     165
 
BstZ17I GTA'TAC

Cuts at:      0     337    1974 
   Size:        337    1637
 
BtsI GCAGTG_nn'

Cuts at:      0    1874    1974 
   Size:       1874     100
 
DraIII CAC_nnn'GTG

Cuts at:      0     883    1974 
   Size:        883    1091
 
EaeI y'GGCC_r

Cuts at:      0      20     725    1974 
   Size:         20     705    1249

  Fragments arranged by size: 

                1249     705      20
 
EagI C'GGCC_G

Cuts at:      0      20    1974 
   Size:         20    1954
 
EarI CTCTTCn'nnn_

Cuts at:      0     434     755     980    1054    1650    1974 
   Size:        434     321     225      74     596     324

  Fragments arranged by size: 

                 596     434     324     321     225      74
 
EcoICRI GAG'CTC

Cuts at:      0     170    1113    1883    1974 
   Size:        170     943     770      91

  Fragments arranged by size: 

                 943     770     170      91
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     133     522    1129    1627    1974 
   Size:        133     389     607     498     347

  Fragments arranged by size: 

                 607     498     389     347     133
 
EcoO109I rG'GnC_Cy

Cuts at:      0      42    1974 
   Size:         42    1932
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     660    1974 
   Size:        660    1314
 
HaeII r_GCGC'y

Cuts at:      0     495    1974 
   Size:        495    1479
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     447     479    1380    1412    1974 
   Size:        447      32     901      32     562

  Fragments arranged by size: 

                 901     562     447      32      32
 
HincII GTy'rAC

Cuts at:      0    1529    1776    1974 
   Size:       1529     247     198

  Fragments arranged by size: 

                1529     247     198
 
KasI G'GCGC_C

Cuts at:      0     491    1974 
   Size:        491    1483
 
MfeI C'AATT_G

Cuts at:      0    1379    1974 
   Size:       1379     595
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1146    1534    1974 
   Size:       1146     388     440

  Fragments arranged by size: 

                1146     440     388
 
MslI CAynn'nnrTG

Cuts at:      0     107    1216    1974 
   Size:        107    1109     758

  Fragments arranged by size: 

                1109     758     107
 
MspA1I CmG'CkG

Cuts at:      0      66     663    1367    1974 
   Size:         66     597     704     607

  Fragments arranged by size: 

                 704     607     597      66
 
NarI GG'CG_CC

Cuts at:      0     492    1974 
   Size:        492    1482
 
NspI r_CATG'y

Cuts at:      0    1444    1548    1974 
   Size:       1444     104     426

  Fragments arranged by size: 

                1444     426     104
 
PciI A'CATG_T

Cuts at:      0    1440    1974 
   Size:       1440     534
 
PfoI T'CCnGG_A

Cuts at:      0    1650    1974 
   Size:       1650     324
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1385    1417    1974 
   Size:       1385      32     557

  Fragments arranged by size: 

                1385     557      32
 
PshAI GACnn'nnGTC

Cuts at:      0     273    1974 
   Size:        273    1701
 
PspOMI G'GGCC_C

Cuts at:      0      41    1974 
   Size:         41    1933
 
PstI C_TGCA'G

Cuts at:      0     198     997    1026    1974 
   Size:        198     799      29     948

  Fragments arranged by size: 

                 948     799     198      29
 
SacI G_AGCT'C

Cuts at:      0     172    1115    1885    1974 
   Size:        172     943     770      89

  Fragments arranged by size: 

                 943     770     172      89
 
SexAI A'CCwGG_T

Cuts at:      0    1251    1974 
   Size:       1251     723
 
SfcI C'TryA_G

Cuts at:      0     194     993    1022    1974 
   Size:        194     799      29     952

  Fragments arranged by size: 

                 952     799     194      29
 
SfoI GGC'GCC

Cuts at:      0     493    1974 
   Size:        493    1481
 
SmaI CCC'GGG

Cuts at:      0    1851    1974 
   Size:       1851     123
 
SpeI A'CTAG_T

Cuts at:      0    1416    1974 
   Size:       1416     558
 
SspI AAT'ATT

Cuts at:      0     237    1431    1974 
   Size:        237    1194     543

  Fragments arranged by size: 

                1194     543     237
 
StyI C'CwwG_G

Cuts at:      0     980    1346    1974 
   Size:        980     366     628

  Fragments arranged by size: 

                 980     628     366
 
TatI w'GTAC_w

Cuts at:      0     680    1634    1974 
   Size:        680     954     340

  Fragments arranged by size: 

                 954     680     340
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      76     108     829     861    1974 
   Size:         76      32     721      32    1113

  Fragments arranged by size: 

                1113     721      76      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1615    1974 
   Size:       1615     359
 
XbaI T'CTAG_A

Cuts at:      0     408    1974 
   Size:        408    1566
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1648    1974 
   Size:       1648     326
 
XmaI C'CCGG_G

Cuts at:      0    1849    1974 
   Size:       1849     125
 
ZraI GAC'GTC

Cuts at:      0     421    1974 
   Size:        421    1553
 

 Enzymes that do cut:

    AatII     AccI     AcuI   AflIII     AleI     AlfI    AlwNI     ApaI
     ApoI     AvaI     BanI    BanII     BbeI     BbsI    BbvCI    BciVI
     BclI     BlpI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I     BsaI
    BsaBI    BsaHI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI    BsmBI
 Bsp1286I    BspHI    BsrFI   BssHII   BstAPI    BstBI    BstYI  BstZ17I
     BtsI   DraIII     EaeI     EagI     EarI  EcoICRI  Eco57MI EcoO109I
     FalI    HaeII    Hin4I   HincII     KasI     MfeI     MmeI     MslI
   MspA1I     NarI     NspI     PciI     PfoI     PpiI    PshAI   PspOMI
     PstI     SacI    SexAI     SfcI     SfoI     SmaI     SpeI     SspI
     StyI     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AloI
    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BcgI
    BfrBI     BglI    BglII     BmtI     BplI    BpuEI    BsaAI    BsaWI
     BsgI    BsiWI     BsmI    BspEI    BspMI    BsrBI    BsrDI    BsrGI
    BssSI   BstEII    BstXI   Bsu36I     BtgI    BtgZI     ClaI    CspCI
     DraI     DrdI     EciI    EcoNI    EcoRI    EcoRV     FseI     FspI
    FspAI  HindIII     HpaI     KpnI     MluI     MscI     NaeI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
    PflMI     PmeI     PmlI    PpuMI     PsiI    PspXI     PsrI     PvuI
    PvuII    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI
     SfiI     SgfI    SgrAI     SmlI    SnaBI     SphI     SrfI     StuI
     SwaI    TaqII     XhoI     XmnI
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