Restriction map for pF1KB5059
##################################
# NO CUT ENZYMES (  108 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AviII       AvrII       BamHI       
BbrPI       BbvCI       BclI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BseAI       BsgI        
BsiHKAI     BsiWI       BsmFI       BspDI       BspEI       
BspHI       BspLU11I    Bst1107I    BstBI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DraI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco52I      Eco81I      EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HincII      HindII      
HindIII     HpaI        KpnI        KspI        MfeI        
MluI        MroI        MunI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PflFI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        SbfI        
SexAI       SfiI        SfuI        SgrAI       SpeI        
SphI        Sse8387I    SspI        SwaI        Tth111I     
XbaI        XcmI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

Aor51HI      1647  AGCGCT               AGC^GCT
ApaI          769  GGGCCC               GGGCC^C
AvaII        1638  GGACC                G^G[AT]CC
BalI          953  TGGCCA               TGG^CCA
BbeI          965  GGCGCC               GGCGC^C
BcgI           42  CGAGGTGCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         524  GTATCC               GTATCC(6/5)
BsaBI         663  GATAGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          922  GAGACC               GGTCTC(1/5)
BsaWI        1553  ACCGGA               [AT]^CCGG[AT]
BsiEI        1827  CGGTCG               CG[GA][CT]^CG
BsmBI         518  GAGACG               CGTCTC(1/5)
BsrBI          63  CCGCTC               CCGCTC(-3/-3)
BsrDI        1699  CATTGC               GCAATG(2/0)
BsrFI         125  GCCGGC               [GA]^CCGG[CT]
BsrI          696  ACTGG                ACTGG(1/-1)
BssHII        298  GCGCGC               G^CGCGC
BssSI        1345  CACGAG               CACGAG(-5/-1)
BstEII        746  GGTTACC              G^GT[ACGT]ACC
BstPI         746  GGTTACC              G^GT[ACGT]ACC
Cfr10I        125  GCCGGC               [GA]^CCGG[CT]
Eco47III     1647  AGCGCT               AGC^GCT
EcoO65I       746  GGTTACC              G^GT[ACGT]ACC
KasI          965  GGCGCC               G^GCGCC
MamI          663  GATAGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         953  TGGCCA               TGG^CCA
MscI          953  TGGCCA               TGG^CCA
MseI          281  TTAA                 T^TAA
NaeI          125  GCCGGC               GCC^GGC
NarI          965  GGCGCC               GG^CGCC
NgoMIV        125  GCCGGC               G^CCGGC
PflMI         501  CCACATCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI        1006  GACATCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI          833  AGTACT               AGT^ACT
SfoI          965  GGCGCC               GGC^GCC
SnaBI         408  TACGTA               TAC^GTA
StuI          582  AGGCCT               AGG^CCT
TfiI         1143  GATTC                G^A[AT]TC
Tru9I         281  TTAA                 T^TAA
Tsp509I      1516  AATT                 ^AATT
Van91I        501  CCACATCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AcyI          965  GGCGCC               G[GA]^CG[CT]C
             1336  GACGCC              
BanII          29  GGGCTC               G[GA]GC[CT]^C
              769  GGGCCC              
BbsI          158  GAAGAC               GAAGAC(2/6)
             1287  GAAGAC              
BglI          111  GCCGTTACGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1420  GCCATCGAGGC         
BpuAI         158  GAAGAC               GAAGAC(2/6)
             1287  GAAGAC              
BsaAI          97  TACGTG               [CT]AC^GT[GA]
              408  TACGTA              
BsaHI         965  GGCGCC               G[GA]^CG[CT]C
             1336  GACGCC              
BsmAI         518  GAGAC                GTCTC(1/5)
              922  GAGAC               
BsmI          599  GCATTC               GAATGC(1/-1)
             1429  GCATTC              
Bsp1407I      939  TGTACA               T^GTACA
             1548  TGTACA              
BsrGI         939  TGTACA               T^GTACA
             1548  TGTACA              
BstXI        1184  CCATCAATGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1478  CCAAGGGCCTGG        
BstYI         344  AGATCC               [GA]^GATC[CT]
              623  AGATCC              
HgaI          161  GACGC                GACGC(5/10)
             1336  GACGC               
Hin1I         965  GGCGCC               G[GA]^CG[CT]C
             1336  GACGCC              
MflI          344  AGATCC               [GA]^GATC[CT]
              623  AGATCC              
PaeR7I       1273  CTCGAG               C^TCGAG
             1792  CTCGAG              
PvuII         325  CAGCTG               CAG^CTG
              432  CAGCTG              
SmaI          209  CCCGGG               CCC^GGG
              960  CCCGGG              
SspBI         939  TGTACA               T^GTACA
             1548  TGTACA              
XhoI         1273  CTCGAG               C^TCGAG
             1792  CTCGAG              
XhoII         344  AGATCC               [GA]^GATC[CT]
              623  AGATCC              
XmaCI         209  CCCGGG               C^CCGGG
              960  CCCGGG              
XmaI          209  CCCGGG               C^CCGGG
              960  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   85 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       AvaI        BanI        BbvI        BcnI        
BfaI        BmyI        BpmI        BsaJI       BseRI       
BsiYI       BslI        BsoBI       Bsp1286I    BspMI       
BssKI       BstAPI      BstF5I      BstNI       BstUI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MnlI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NcoI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PstI        RsaI        SapI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StyI        
TaqI        TseI        Tsp45I      TspRI       TthHB8I     
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