Restriction map for pF1KB5059
 (Linear) (Six-base) MAPSORT of: KIBB5059.seq  Check: 5634  from: 1  to: 1866



KIBB5059.orf

 With 182 enzymes: SgfI * 

                        February 15, 2010 17:50  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1680    1866 
   Size:       1680     186
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     314     349     488     607    1104    1866 
   Size:        314      35     139     119     497     762

  Fragments arranged by size: 

                 762     497     314     139     119      35
 
AfeI AGC'GCT

Cuts at:      0    1649    1866 
   Size:       1649     217
 
AlwNI CAG_nnn'CTG

Cuts at:      0     135     147    1113    1695    1866 
   Size:        135      12     966     582     171

  Fragments arranged by size: 

                 966     582     171     135      12
 
ApaI G_GGCC'C

Cuts at:      0     773    1866 
   Size:        773    1093
 
AvaI C'yCGr_G

Cuts at:      0     209     960    1273    1575    1792    1866 
   Size:        209     751     313     302     217      74

  Fragments arranged by size: 

                 751     313     302     217     209      74
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1059    1092    1866 
   Size:       1059      33     774

  Fragments arranged by size: 

                1059     774      33
 
BanI G'GyrC_C

Cuts at:      0      45     965    1674    1866 
   Size:         45     920     709     192

  Fragments arranged by size: 

                 920     709     192      45
 
BanII G_rGCy'C

Cuts at:      0      33     773    1866 
   Size:         33     740    1093

  Fragments arranged by size: 

                1093     740      33
 
BbeI G_GCGC'C

Cuts at:      0     969    1866 
   Size:        969     897
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     165    1294    1866 
   Size:        165    1129     572

  Fragments arranged by size: 

                1129     572     165
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      31      65    1866 
   Size:         31      34    1801

  Fragments arranged by size: 

                1801      34      31
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     535    1866 
   Size:        535    1331
 
BglI GCCn_nnn'nGGC

Cuts at:      0     117    1426    1866 
   Size:        117    1309     440

  Fragments arranged by size: 

                1309     440     117
 
Bme1580I G_kGCm'C

Cuts at:      0     104     773    1167    1866 
   Size:        104     669     394     699

  Fragments arranged by size: 

                 699     669     394     104
 
BmgBI CAC'GTC

Cuts at:      0    1332    1509    1866 
   Size:       1332     177     357

  Fragments arranged by size: 

                1332     357     177
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     879    1866 
   Size:        879     987
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     124     370     850    1866 
   Size:        124     246     480    1016

  Fragments arranged by size: 

                1016     480     246     124
 
Bpu10I CC'TnA_GC

Cuts at:      0     398    1866 
   Size:        398    1468
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1345    1866 
   Size:       1345     521
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     916    1866 
   Size:        916     950
 
BsaAI yAC'GTr

Cuts at:      0      99     410    1866 
   Size:         99     311    1456

  Fragments arranged by size: 

                1456     311      99
 
BsaBI GATnn'nnATC

Cuts at:      0     667    1866 
   Size:        667    1199
 
BsaHI Gr'CG_yC

Cuts at:      0     966    1337    1866 
   Size:        966     371     529

  Fragments arranged by size: 

                 966     529     371
 
BsaWI w'CCGG_w

Cuts at:      0    1553    1866 
   Size:       1553     313
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1459    1489    1866 
   Size:       1459      30     377

  Fragments arranged by size: 

                1459     377      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      19    1264    1546    1745    1866 
   Size:         19    1245     282     199     121

  Fragments arranged by size: 

                1245     282     199     121      19
 
BseYI C'CCAG_C

Cuts at:      0    1201    1866 
   Size:       1201     665
 
BsiEI CG_ry'CG

Cuts at:      0    1830    1866 
   Size:       1830      36
 
BsmI GAATG_Cn'

Cuts at:      0     599    1429    1866 
   Size:        599     830     437

  Fragments arranged by size: 

                 830     599     437
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     512    1866 
   Size:        512    1354
 
Bsp1286I G_dGCh'C

Cuts at:      0      33     104     773    1167    1866 
   Size:         33      71     669     394     699

  Fragments arranged by size: 

                 699     669     394      71      33
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1277    1361    1680    1803    1866 
   Size:       1277      84     319     123      63

  Fragments arranged by size: 

                1277     319     123      84      63
 
BsrBI CCG'CTC

Cuts at:      0      65    1866 
   Size:         65    1801
 
BsrDI GCAATG_nn'

Cuts at:      0    1698    1866 
   Size:       1698     168
 
BsrFI r'CCGG_y

Cuts at:      0     125    1866 
   Size:        125    1741
 
BsrGI T'GTAC_A

Cuts at:      0     939    1548    1866 
   Size:        939     609     318

  Fragments arranged by size: 

                 939     609     318
 
BssHII G'CGCG_C

Cuts at:      0     298    1866 
   Size:        298    1568
 
BssSI C'ACGA_G

Cuts at:      0    1345    1866 
   Size:       1345     521
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     135    1674    1695    1866 
   Size:        135    1539      21     171

  Fragments arranged by size: 

                1539     171     135      21
 
BstEII G'GTnAC_C

Cuts at:      0     746    1866 
   Size:        746    1120
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1191    1485    1866 
   Size:       1191     294     381

  Fragments arranged by size: 

                1191     381     294
 
BstYI r'GATC_y

Cuts at:      0     344     623    1866 
   Size:        344     279    1243

  Fragments arranged by size: 

                1243     344     279
 
BtgI C'CryG_G

Cuts at:      0     819    1386    1856    1866 
   Size:        819     567     470      10

  Fragments arranged by size: 

                 819     567     470      10
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     654    1575    1866 
   Size:        654     921     291

  Fragments arranged by size: 

                 921     654     291
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1452    1487    1866 
   Size:       1452      35     379

  Fragments arranged by size: 

                1452     379      35
 
EaeI y'GGCC_r

Cuts at:      0     651     953    1560    1866 
   Size:        651     302     607     306

  Fragments arranged by size: 

                 651     607     306     302
 
EarI CTCTTCn'nnn_

Cuts at:      0     176     332     464     736    1123    1866 
   Size:        176     156     132     272     387     743

  Fragments arranged by size: 

                 743     387     272     176     156     132
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      88    1866 
   Size:         88    1778
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     124     314     349     370     488     607     850    1104 
   Size:        124     190      35      21     118     119     243     254

Cuts at:   1104    1866 
   Size:        762

  Fragments arranged by size: 

                 762     254     243     190     124     119     118      35
                  21
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      53     511     850    1030    1866 
   Size:         53     458     339     180     836

  Fragments arranged by size: 

                 836     458     339     180      53
 
EcoO109I rG'GnC_Cy

Cuts at:      0     238     769     770    1482    1758    1866 
   Size:        238     531       1     712     276     108

  Fragments arranged by size: 

                 712     531     276     238     108       1
 
HaeII r_GCGC'y

Cuts at:      0     126     969    1575    1651    1866 
   Size:        126     843     606      76     215

  Fragments arranged by size: 

                 843     606     215     126      76
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     509     541     770     802    1459    1491    1866 
   Size:        509      32     229      32     657      32     375

  Fragments arranged by size: 

                 657     509     375     229      32      32      32
 
KasI G'GCGC_C

Cuts at:      0     965    1866 
   Size:        965     901
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     272     954    1866 
   Size:        272     682     912

  Fragments arranged by size: 

                 912     682     272
 
MscI TGG'CCA

Cuts at:      0     955    1866 
   Size:        955     911
 
MslI CAynn'nnrTG

Cuts at:      0     508     904     949    1189    1866 
   Size:        508     396      45     240     677

  Fragments arranged by size: 

                 677     508     396     240      45
 
MspA1I CmG'CkG

Cuts at:      0     197     327     434    1866 
   Size:        197     130     107    1432

  Fragments arranged by size: 

                1432     197     130     107
 
NaeI GCC'GGC

Cuts at:      0     127    1866 
   Size:        127    1739
 
NarI GG'CG_CC

Cuts at:      0     966    1866 
   Size:        966     900
 
NcoI C'CATG_G

Cuts at:      0     819    1386    1856    1866 
   Size:        819     567     470      10

  Fragments arranged by size: 

                 819     567     470      10
 
NgoMIV G'CCGG_C

Cuts at:      0     125    1866 
   Size:        125    1741
 
NspI r_CATG'y

Cuts at:      0     465     903    1527    1866 
   Size:        465     438     624     339

  Fragments arranged by size: 

                 624     465     438     339
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     507    1866 
   Size:        507    1359
 
PfoI T'CCnGG_A

Cuts at:      0     260     347     827    1226    1433    1866 
   Size:        260      87     480     399     207     433

  Fragments arranged by size: 

                 480     433     399     260     207      87
 
PshAI GACnn'nnGTC

Cuts at:      0    1010    1866 
   Size:       1010     856
 
PspOMI G'GGCC_C

Cuts at:      0     769    1866 
   Size:        769    1097
 
PspXI vC'TCGA_Gb

Cuts at:      0    1273    1792    1866 
   Size:       1273     519      74

  Fragments arranged by size: 

                1273     519      74
 
PstI C_TGCA'G

Cuts at:      0     155     570     854     986    1286    1691    1866 
   Size:        155     415     284     132     300     405     175

  Fragments arranged by size: 

                 415     405     300     284     175     155     132
 
PvuII CAG'CTG

Cuts at:      0     327     434    1866 
   Size:        327     107    1432

  Fragments arranged by size: 

                1432     327     107
 
SapI GCTCTTCn'nnn_

Cuts at:      0     176     464    1123    1866 
   Size:        176     288     659     743

  Fragments arranged by size: 

                 743     659     288     176
 
ScaI AGT'ACT

Cuts at:      0     835    1866 
   Size:        835    1031
 
SfcI C'TryA_G

Cuts at:      0     151     566     850     982    1282    1687    1866 
   Size:        151     415     284     132     300     405     179

  Fragments arranged by size: 

                 415     405     300     284     179     151     132
 
SfoI GGC'GCC

Cuts at:      0     967    1866 
   Size:        967     899
 
SmaI CCC'GGG

Cuts at:      0     211     962    1866 
   Size:        211     751     904

  Fragments arranged by size: 

                 904     751     211
 
SmlI C'TyrA_G

Cuts at:      0    1273    1360    1792    1866 
   Size:       1273      87     432      74

  Fragments arranged by size: 

                1273     432      87      74
 
SnaBI TAC'GTA

Cuts at:      0     410    1866 
   Size:        410    1456
 
StuI AGG'CCT

Cuts at:      0     584    1866 
   Size:        584    1282
 
StyI C'CwwG_G

Cuts at:      0     314     819    1386    1478    1856    1866 
   Size:        314     505     567      92     378      10

  Fragments arranged by size: 

                 567     505     378     314      92      10
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     549     623     630    1816    1866 
   Size:        549      74       7    1186      50

  Fragments arranged by size: 

                1186     549      74      50       7
 
TatI w'GTAC_w

Cuts at:      0     833     939    1302    1548    1866 
   Size:        833     106     363     246     318

  Fragments arranged by size: 

                 833     363     318     246     106
 
XhoI C'TCGA_G

Cuts at:      0    1273    1792    1866 
   Size:       1273     519      74

  Fragments arranged by size: 

                1273     519      74
 
XmaI C'CCGG_G

Cuts at:      0     209     960    1866 
   Size:        209     751     906

  Fragments arranged by size: 

                 906     751     209
 

 Enzymes that do cut:

     AarI     AcuI     AfeI    AlwNI     ApaI     AvaI     BaeI     BanI
    BanII     BbeI     BbsI     BcgI    BciVI     BglI Bme1580I    BmgBI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI    BsiEI     BsmI    BsmBI Bsp1286I
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
   BstEII    BstXI    BstYI     BtgI    BtgZI    CspCI     EaeI     EarI
     EciI  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I     KasI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI
    PflMI     PfoI    PshAI   PspOMI    PspXI     PstI    PvuII     SapI
     ScaI     SfcI     SfoI     SmaI     SmlI    SnaBI     StuI     StyI
    TaqII     TatI     XhoI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII    BamHI    BbvCI     BclI    BfrBI    BglII     BlpI     BmrI
     BmtI     BsgI  BsiHKAI    BsiWI    BspEI    BspHI    BstBI  BstZ17I
   Bsu36I     BtsI     ClaI     DraI   DraIII     DrdI     EagI  EcoICRI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI     PmeI     PmlI     PpiI    PpuMI
     PsiI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SbfI    SexAI     SfiI     SgfI    SgrAI     SpeI     SphI     SrfI
     SspI     SwaI     TstI  Tth111I     XbaI     XcmI     XmnI     ZraI
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