Restriction map for pF1KB9284
 (Linear) (Six-base) MAPSORT of: KIBB9284.seq  Check: 1119  from: 1  to: 585



KIBB9284.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:26  ..

Acc65I G'GTAC_C

Cuts at:      0     475     585 
   Size:        475     110
 
AflIII A'CryG_T

Cuts at:      0     119     585 
   Size:        119     466
 
AleI CACnn'nnGTG

Cuts at:      0     358     361     585 
   Size:        358       3     224

  Fragments arranged by size: 

                 358     224       3
 
AlwNI CAG_nnn'CTG

Cuts at:      0     103     321     585 
   Size:        103     218     264

  Fragments arranged by size: 

                 264     218     103
 
ApaI G_GGCC'C

Cuts at:      0     546     585 
   Size:        546      39
 
BanI G'GyrC_C

Cuts at:      0     475     585 
   Size:        475     110
 
BanII G_rGCy'C

Cuts at:      0     286     546     585 
   Size:        286     260      39

  Fragments arranged by size: 

                 286     260      39
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     134     585 
   Size:        134     451
 
Bme1580I G_kGCm'C

Cuts at:      0     533     546     575     585 
   Size:        533      13      29      10

  Fragments arranged by size: 

                 533      29      13      10
 
BmgBI CAC'GTC

Cuts at:      0     342     585 
   Size:        342     243
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     149     585 
   Size:        149     436
 
BsaAI yAC'GTr

Cuts at:      0     305     536     585 
   Size:        305     231      49

  Fragments arranged by size: 

                 305     231      49
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     208     238     585 
   Size:        208      30     347

  Fragments arranged by size: 

                 347     208      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     210     213     326     585 
   Size:        210       3     113     259

  Fragments arranged by size: 

                 259     210     113       3
 
BseYI C'CCAG_C

Cuts at:      0     275     448     585 
   Size:        275     173     137

  Fragments arranged by size: 

                 275     173     137
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     385     585 
   Size:        385     200
 
Bsp1286I G_dGCh'C

Cuts at:      0     286     533     546     575     585 
   Size:        286     247      13      29      10

  Fragments arranged by size: 

                 286     247      29      13      10
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     463     585 
   Size:        463     122
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     103     585 
   Size:        103     482
 
BstEII G'GTnAC_C

Cuts at:      0     429     585 
   Size:        429     156
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     441     585 
   Size:        441     144
 
BstYI r'GATC_y

Cuts at:      0      22     585 
   Size:         22     563
 
BtgI C'CryG_G

Cuts at:      0      80     356     434     458     585 
   Size:         80     276      78      24     127

  Fragments arranged by size: 

                 276     127      80      78      24
 
EaeI y'GGCC_r

Cuts at:      0     461     585 
   Size:        461     124
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     149     585 
   Size:        149     436
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     416     585 
   Size:        416     169
 
EcoO109I rG'GnC_Cy

Cuts at:      0     543     585 
   Size:        543      42
 
KpnI G_GTAC'C

Cuts at:      0     479     585 
   Size:        479     106
 
MscI TGG'CCA

Cuts at:      0     463     585 
   Size:        463     122
 
MslI CAynn'nnrTG

Cuts at:      0     358     361     439     463     585 
   Size:        358       3      78      24     122

  Fragments arranged by size: 

                 358     122      78      24       3
 
MspA1I CmG'CkG

Cuts at:      0      70     200     321     585 
   Size:         70     130     121     264

  Fragments arranged by size: 

                 264     130     121      70
 
NcoI C'CATG_G

Cuts at:      0      80     434     458     585 
   Size:         80     354      24     127

  Fragments arranged by size: 

                 354     127      80      24
 
NspI r_CATG'y

Cuts at:      0     123     381     585 
   Size:        123     258     204

  Fragments arranged by size: 

                 258     204     123
 
PciI A'CATG_T

Cuts at:      0     119     585 
   Size:        119     466
 
PfoI T'CCnGG_A

Cuts at:      0      18     126     585 
   Size:         18     108     459

  Fragments arranged by size: 

                 459     108      18
 
PspOMI G'GGCC_C

Cuts at:      0     542     585 
   Size:        542      43
 
PvuII CAG'CTG

Cuts at:      0      70     585 
   Size:         70     515
 
SphI G_CATG'C

Cuts at:      0     381     585 
   Size:        381     204
 
StyI C'CwwG_G

Cuts at:      0      80     434     458     585 
   Size:         80     354      24     127

  Fragments arranged by size: 

                 354     127      80      24
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     441     585 
   Size:        441     144
 

 Enzymes that do cut:

   Acc65I   AflIII     AleI    AlwNI     ApaI     BanI    BanII    BciVI
 Bme1580I    BmgBI     BpmI    BsaAI    BsaXI    BseRI    BseYI     BsgI
 Bsp1286I    BspMI   BstAPI   BstEII    BstXI    BstYI     BtgI     EaeI
  Eco57MI    EcoNI EcoO109I     KpnI     MscI     MslI   MspA1I     NcoI
     NspI     PciI     PfoI   PspOMI    PvuII     SphI     StyI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI     BcgI
     BclI    BfrBI     BglI    BglII     BlpI     BmrI     BmtI     BplI
   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI    BstBI  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI
  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     MfeI     MluI     MmeI
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI
     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI
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