Restriction map for pF1KB9044
##################################
# NO CUT ENZYMES (  173 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AfaI        AflII       AflIII      
AhdI        Alw44I      AlwI        AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AvaI        
AviII       AvrII       BamHI       BanI        BbeI        
BbrPI       BbvCI       BbvI        BcgI        BciVI       
BclI        BcnI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BsaBI       BsaHI       
BsaI        BseAI       BsiEI       BsiWI       BsmBI       
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstUI       BstXI       BstYI       BstZ17I     Bsu36I      
CelII       CfoI        ClaI        CpoI        DpnII       
DraI        DraII       DraIII      DrdI        DsaI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoO109I    EcoO65I     EcoRV       EcoT14I     
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HpaI        KasI        KpnI        
KspI        MamI        MboI        MfeI        MflI        
MluI        MroI        MspA1I      MunI        MvnI        
NaeI        NarI        NciI        NcoI        NdeII       
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsaI        RsrII       SacI        SacII       
SalI        SapI        Sau3AI      SbfI        ScaI        
SfaNI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        Sse8387I    
SspBI       StuI        StyI        SwaI        TseI        
Tth111I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AciI         1148  GCGG                 CCGC(-3/-1)
AgeI         1499  ACCGGT               A^CCGGT
AspHI        1251  GTGCTC               G[AT]GC[AT]^C
BanII         208  GAGCCC               G[GA]GC[CT]^C
BsaAI         475  TACGTG               [CT]AC^GT[GA]
BsaWI        1499  ACCGGT               [AT]^CCGG[AT]
BseRI          73  GAGGAG               GAGGAG(10/8)
BsgI           95  CTGCAC               GTGCAG(16/14)
BsiHKAI      1251  GTGCTC               G[AT]GC[AT]^C
BsmFI          30  GGGAC                GGGAC(10/14)
BsmI         1144  GAATGC               GAATGC(1/-1)
BsrFI        1499  ACCGGT               [GA]^CCGG[CT]
BstAPI       1423  GCAACTCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I       1499  ACCGGT               [GA]^CCGG[CT]
EcoNI         212  CCTGGAATAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Fnu4HI       1148  GCGGC                GC^[ACGT]GC
HapII        1500  CCGG                 C^CGG
HpaII        1500  CCGG                 C^CGG
ItaI         1148  GCGGC                GC^[ACGT]GC
MaeII         476  ACGT                 A^CGT
MaeIII        896  GTCAC                ^GT[ACGT]AC
MslI         1534  CATACTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI         1500  CCGG                 C^CGG
MwoI         1423  GCAACTCTTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV          30  GGGACC               GG[ACGT]^[ACGT]CC
NspI          575  GCATGC               [GA]CATG^[CT]
PinAI        1499  ACCGGT               A^CCGGT
PleI          391  GAGTC                GAGTC(4/5)
SexAI         143  ACCTGGT              A^CC[AT]GGT
SfcI          135  CTACAG               C^T[GA][CT]AG
SphI          575  GCATGC               GCATG^C
SspI          456  AATATT               AAT^ATT
TaqI         1266  TCGA                 T^CGA
TfiI          304  GATTC                G^A[AT]TC
Tsp45I        896  GTCAC                ^GT[GC]AC
TthHB8I      1266  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

BalI           53  TGGCCA               TGG^CCA
              283  TGGCCA              
BbsI          274  GAAGAC               GAAGAC(2/6)
              148  GTCTTC              
BmyI          208  GAGCCC               G[AGT]GC[ACT]^C
             1251  GTGCTC              
BpuAI         274  GAAGAC               GAAGAC(2/6)
              148  GTCTTC              
BsaJI         153  CCTGGG               C^C[ACGT][ACGT]GG
              211  CCCTGG              
Bsp1286I      208  GAGCCC               G[AGT]GC[ACT]^C
             1251  GTGCTC              
BsrDI          81  GCAATG               GCAATG(2/0)
             1403  GCAATG              
BstF5I         48  GGATG                GGATG(2/0)
              387  GGATG               
Cac8I         575  GCATGC               GC[ACGT]^[ACGT]GC
             1419  GCCTGC              
EaeI           53  TGGCCA               [CT]^GGCC[GA]
              283  TGGCCA              
EarI          222  GAAGAG               CTCTTC(1/4)
              784  GAAGAG              
FokI           48  GGATG                GGATG(9/13)
              387  GGATG               
HinfI         304  GATTC                G^A[ACGT]TC
              391  GAGTC               
HphI         1540  GGTGA                GGTGA(8/7)
              177  TCACC               
Ksp632I       222  GAAGAG               CTCTTC(1/4)
              784  GAAGAG              
MluNI          53  TGGCCA               TGG^CCA
              283  TGGCCA              
MscI           53  TGGCCA               TGG^CCA
              283  TGGCCA              
NdeI          694  CATATG               CA^TATG
             1186  CATATG              


#########################################
# 3 OR MORE CUT ENZYMES (   31 enzymes) #
#########################################

AcsI        AluI        ApoI        Asp700I     AvaII       
BfaI        BpmI        BsiYI       BslI        BsmAI       
BsrI        BssKI       BstNI       Cfr13I      DdeI        
EcoRI       EcoRII      HaeIII      HindIII     MaeI        
MboII       MnlI        MseI        MvaI        NlaIII      
Sau96I      ScrFI       Tru9I       Tsp509I     TspRI       
XmnI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com