Restriction map for pF1KB8733
##################################
# NO CUT ENZYMES (  136 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AcyI        AflIII      AgeI        AhdI        
Alw44I      Aor51HI     ApaLI       ApoI        AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BanI        BbeI        BbrPI       BcgI        BclI        
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrDI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstUI       BstZ17I     CelII       ClaI        
CpoI        DraI        DraIII      DrdI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        Hin1I       HpaI        KasI        
KpnI        KspI        MaeII       MamI        MfeI        
MluI        MluNI       MroI        MscI        MunI        
MvnI        NarI        NdeI        NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacII       SbfI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TfiI        Tth111I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AccI         1082  GTCGAC               GT^[AC][GT]AC
AflII         775  CTTAAG               C^TTAAG
AlwNI         487  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          407  GGGCCC               GGGCC^C
Asp700I       592  GAAGAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         541  GAGCTC               G[AT]GC[AT]^C
BbsI          816  GAAGAC               GAAGAC(2/6)
BbvCI         339  CCTCAGC              CCTCAGC(-5/-2)
BciVI         838  GTATCC               GTATCC(6/5)
BfrI          775  CTTAAG               C^TTAAG
BglII         575  AGATCT               A^GATCT
BpuAI         816  GAAGAC               GAAGAC(2/6)
BseRI         523  GAGGAG               GAGGAG(10/8)
BsgI         1427  CTGCAC               GTGCAG(16/14)
BsiHKAI       541  GAGCTC               G[AT]GC[AT]^C
BsmI         1422  GAATGC               GAATGC(1/-1)
BspMI         624  GCAGGT               ACCTGC(4/8)
BsrBI        1338  CCGCTC               CCGCTC(-3/-3)
BsrFI        1306  GCCGGC               [GA]^CCGG[CT]
CfoI          708  GCGC                 GCG^C
Cfr10I       1306  GCCGGC               [GA]^CCGG[CT]
DsaI          821  CCATGG               C^C[GA][CT]GG
EcoNI         963  CCTGTCAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII         707  AGCGCC               [GA]GCGC^[CT]
HhaI          708  GCGC                 GCG^C
HinP1I        708  GCGC                 G^CGC
HindIII       149  AAGCTT               A^AGCTT
NaeI         1306  GCCGGC               GCC^GGC
NcoI          821  CCATGG               C^CATGG
NgoMIV       1306  GCCGGC               G^CCGGC
PvuII          47  CAGCTG               CAG^CTG
SacI          541  GAGCTC               GAGCT^C
SalI         1082  GTCGAC               G^TCGAC
ScaI          881  AGTACT               AGT^ACT
SpeI          552  ACTAGT               A^CTAGT
XmnI          592  GAAGAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AocI          891  CCTAAGG              CC^T[ACGT]AGG
             1076  CCTCAGG             
BglI          721  GCCCACTTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1154  GCCGAAAGGGC         
BstAPI        146  GCAAAGCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              624  GCAGGTGTTGC         
BstXI         842  CCATAGAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1450  CCAACAGATTGG        
Bsu36I        891  CCTAAGG              CC^T[ACGT]AGG
             1076  CCTCAGG             
Eco81I        891  CCTAAGG              CC^T[ACGT]AGG
             1076  CCTCAGG             
HgaI          983  GCGTC                GACGC(5/10)
             1052  GCGTC               
HincII       1082  GTCGAC               GT[CT]^[GA]AC
             1167  GTTGAC              
HindII       1082  GTCGAC               GT[CT]^[GA]AC
             1167  GTTGAC              
MseI          776  TTAA                 T^TAA
              905  TTAA                
MspA1I         47  CAGCTG               C[AC]G^C[GT]G
              162  CCGCTG              
NlaIII        331  CATG                 CATG^
              822  CATG                
PpuMI         173  AGGACCT              [GA]G^G[AT]CC[CT]
              713  GGGACCT             
SapI          592  GAAGAGC              GCTCTTC(1/4)
              616  GAAGAGC             
Tru9I         776  TTAA                 T^TAA
              905  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   71 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AvaII       
BanII       BbvI        BcnI        BfaI        BmyI        
BpmI        BsaJI       BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       DraII       EarI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeIII      MboI        MboII       
MflI        MnlI        MslI        MspI        MvaI        
MwoI        NciI        NdeII       NlaIV       PleI        
PstI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StyI        TaqI        
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII       
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