Restriction map for pF1KB8733
 (Linear) (Six-base) MAPSORT of: KIBB8733.seq  Check: 2801  from: 1  to: 1464



KIBB8733.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:29  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     615    1464 
   Size:        615     849
 
AccI GT'mk_AC

Cuts at:      0    1083    1464 
   Size:       1083     381
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     127     818     846    1464 
   Size:        127     691      28     618

  Fragments arranged by size: 

                 691     618     127      28
 
AflII C'TTAA_G

Cuts at:      0     775    1464 
   Size:        775     689
 
AleI CACnn'nnGTG

Cuts at:      0     418    1464 
   Size:        418    1046
 
AlwNI CAG_nnn'CTG

Cuts at:      0     492    1464 
   Size:        492     972
 
ApaI G_GGCC'C

Cuts at:      0     411    1464 
   Size:        411    1053
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     821     854    1464 
   Size:        821      33     610

  Fragments arranged by size: 

                 821     610      33
 
BanII G_rGCy'C

Cuts at:      0     411     479     545    1464 
   Size:        411      68      66     919

  Fragments arranged by size: 

                 919     411      68      66
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     823    1464 
   Size:        823     641
 
BbvCI CC'TCA_GC

Cuts at:      0     340    1464 
   Size:        340    1124
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     849    1464 
   Size:        849     615
 
BglI GCCn_nnn'nGGC

Cuts at:      0     727    1160    1464 
   Size:        727     433     304

  Fragments arranged by size: 

                 727     433     304
 
BglII A'GATC_T

Cuts at:      0     575    1464 
   Size:        575     889
 
Bme1580I G_kGCm'C

Cuts at:      0     137     411    1464 
   Size:        137     274    1053

  Fragments arranged by size: 

                1053     274     137
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1101    1131    1464 
   Size:       1101      30     333

  Fragments arranged by size: 

                1101     333      30
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     118     265     493    1464 
   Size:        118     147     228     971

  Fragments arranged by size: 

                 971     228     147     118
 
Bpu10I CC'TnA_GC

Cuts at:      0     340    1464 
   Size:        340    1124
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     529     600    1464 
   Size:        529      71     864

  Fragments arranged by size: 

                 864     529      71
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     733     763    1464 
   Size:        733      30     701

  Fragments arranged by size: 

                 733     701      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     538    1464 
   Size:        538     926
 
BseYI C'CCAG_C

Cuts at:      0      25    1464 
   Size:         25    1439
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1412    1464 
   Size:       1412      52
 
BsiHKAI G_wGCw'C

Cuts at:      0     545    1464 
   Size:        545     919
 
BsmI GAATG_Cn'

Cuts at:      0    1428    1464 
   Size:       1428      36
 
Bsp1286I G_dGCh'C

Cuts at:      0     137     411     479     545    1464 
   Size:        137     274      68      66     919

  Fragments arranged by size: 

                 919     274     137      68      66
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     615    1464 
   Size:        615     849
 
BsrBI CCG'CTC

Cuts at:      0    1340    1464 
   Size:       1340     124
 
BsrFI r'CCGG_y

Cuts at:      0    1306    1464 
   Size:       1306     158
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     152     630    1464 
   Size:        152     478     834

  Fragments arranged by size: 

                 834     478     152
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     849    1457    1464 
   Size:        849     608       7

  Fragments arranged by size: 

                 849     608       7
 
BstYI r'GATC_y

Cuts at:      0     299     575    1011    1464 
   Size:        299     276     436     453

  Fragments arranged by size: 

                 453     436     299     276
 
Bsu36I CC'TnA_GG

Cuts at:      0     892    1077    1464 
   Size:        892     185     387

  Fragments arranged by size: 

                 892     387     185
 
BtgI C'CryG_G

Cuts at:      0     821    1464 
   Size:        821     643
 
BtsI GCAGTG_nn'

Cuts at:      0    1133    1464 
   Size:       1133     331
 
EarI CTCTTCn'nnn_

Cuts at:      0     587     611     644    1396    1464 
   Size:        587      24      33     752      68

  Fragments arranged by size: 

                 752     587      68      33      24
 
EcoICRI GAG'CTC

Cuts at:      0     543    1464 
   Size:        543     921
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     118     127     265     493     818     846    1464 
   Size:        118       9     138     228     325      28     618

  Fragments arranged by size: 

                 618     325     228     138     118      28       9
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     967    1464 
   Size:        967     497
 
EcoO109I rG'GnC_Cy

Cuts at:      0      29     174     336     714    1445    1464 
   Size:         29     145     162     378     731      19

  Fragments arranged by size: 

                 731     378     162     145      29      19
 
HaeII r_GCGC'y

Cuts at:      0     711    1464 
   Size:        711     753
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     560     592     933     965    1464 
   Size:        560      32     341      32     499

  Fragments arranged by size: 

                 560     499     341      32      32
 
HincII GTy'rAC

Cuts at:      0    1084    1169    1464 
   Size:       1084      85     295

  Fragments arranged by size: 

                1084     295      85
 
HindIII A'AGCT_T

Cuts at:      0     149    1464 
   Size:        149    1315
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     258    1464 
   Size:        258    1206
 
MslI CAynn'nnrTG

Cuts at:      0     418     826    1264    1464 
   Size:        418     408     438     200

  Fragments arranged by size: 

                 438     418     408     200
 
MspA1I CmG'CkG

Cuts at:      0      49     164    1464 
   Size:         49     115    1300

  Fragments arranged by size: 

                1300     115      49
 
NaeI GCC'GGC

Cuts at:      0    1308    1464 
   Size:       1308     156
 
NcoI C'CATG_G

Cuts at:      0     821    1464 
   Size:        821     643
 
NgoMIV G'CCGG_C

Cuts at:      0    1306    1464 
   Size:       1306     158
 
PfoI T'CCnGG_A

Cuts at:      0     302    1007    1464 
   Size:        302     705     457

  Fragments arranged by size: 

                 705     457     302
 
PpuMI rG'GwC_Cy

Cuts at:      0     174     714    1464 
   Size:        174     540     750

  Fragments arranged by size: 

                 750     540     174
 
PspOMI G'GGCC_C

Cuts at:      0     407    1464 
   Size:        407    1057
 
PstI C_TGCA'G

Cuts at:      0      39     464     497     671    1464 
   Size:         39     425      33     174     793

  Fragments arranged by size: 

                 793     425     174      39      33
 
PvuII CAG'CTG

Cuts at:      0      49    1464 
   Size:         49    1415
 
SacI G_AGCT'C

Cuts at:      0     545    1464 
   Size:        545     919
 
SalI G'TCGA_C

Cuts at:      0    1082    1464 
   Size:       1082     382
 
SapI GCTCTTCn'nnn_

Cuts at:      0     587     611    1464 
   Size:        587      24     853

  Fragments arranged by size: 

                 853     587      24
 
ScaI AGT'ACT

Cuts at:      0     883    1464 
   Size:        883     581
 
SfcI C'TryA_G

Cuts at:      0      35      44     460     493     667    1464 
   Size:         35       9     416      33     174     797

  Fragments arranged by size: 

                 797     416     174      35      33       9
 
SmlI C'TyrA_G

Cuts at:      0     544     579     775    1464 
   Size:        544      35     196     689

  Fragments arranged by size: 

                 689     544     196      35
 
SpeI A'CTAG_T

Cuts at:      0     552    1464 
   Size:        552     912
 
StyI C'CwwG_G

Cuts at:      0     696     821    1027    1464 
   Size:        696     125     206     437

  Fragments arranged by size: 

                 696     437     206     125
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     415    1120    1464 
   Size:        415     705     344

  Fragments arranged by size: 

                 705     415     344
 
TatI w'GTAC_w

Cuts at:      0     446     881    1464 
   Size:        446     435     583

  Fragments arranged by size: 

                 583     446     435
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     733     765    1464 
   Size:        733      32     699

  Fragments arranged by size: 

                 733     699      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     596    1464 
   Size:        596     868
 

 Enzymes that do cut:

     AarI     AccI     AcuI    AflII     AleI    AlwNI     ApaI     BaeI
    BanII     BbsI    BbvCI    BciVI     BglI    BglII Bme1580I     BmrI
     BpmI   Bpu10I    BpuEI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
     BsmI Bsp1286I    BspMI    BsrBI    BsrFI   BstAPI    BstXI    BstYI
   Bsu36I     BtgI     BtsI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I
    HaeII    Hin4I   HincII  HindIII     MmeI     MslI   MspA1I     NaeI
     NcoI   NgoMIV     PfoI    PpuMI   PspOMI     PstI    PvuII     SacI
     SalI     SapI     ScaI     SfcI     SmlI     SpeI     StyI    TaqII
     TatI     TstI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI   AflIII     AgeI     AhdI     AlfI
     AloI    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII
    BamHI     BanI     BbeI     BcgI     BclI    BfrBI     BlpI    BmgBI
     BmtI     BplI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsiEI
    BsiWI    BsmBI    BspEI    BspHI    BsrDI    BsrGI   BssHII    BssSI
    BstBI   BstEII  BstZ17I    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EciI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI     HpaI     KasI     KpnI     MfeI     MluI     MscI
     NarI     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI
    PspXI     PsrI     PvuI    RsrII    SacII    SanDI     SbfI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SphI     SrfI
     SspI     StuI     SwaI  Tth111I     XbaI     XcmI     XhoI     XmaI
     ZraI
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