(Linear) (Six-base) MAPSORT of: KIEE1003.seq  Check: 7219  from: 1  to: 459



KIEE1003.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:28  ..

AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      49     459 
   Size:         49     410
 
BamHI G'GATC_C

Cuts at:      0     113     459 
   Size:        113     346
 
BanI G'GyrC_C

Cuts at:      0     135     459 
   Size:        135     324
 
BanII G_rGCy'C

Cuts at:      0     231     459 
   Size:        231     228
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      45     446     459 
   Size:         45     401      13

  Fragments arranged by size: 

                 401      45      13
 
BbvCI CC'TCA_GC

Cuts at:      0     298     459 
   Size:        298     161
 
BclI T'GATC_A

Cuts at:      0     206     459 
   Size:        206     253
 
Bme1580I G_kGCm'C

Cuts at:      0     140     459 
   Size:        140     319
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      32     459 
   Size:         32     427
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     334     459 
   Size:        334     125
 
Bpu10I CC'TnA_GC

Cuts at:      0     298     459 
   Size:        298     161
 
BsaAI yAC'GTr

Cuts at:      0     417     459 
   Size:        417      42
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     117     459 
   Size:        117     342
 
BseYI C'CCAG_C

Cuts at:      0     158     459 
   Size:        158     301
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     261     459 
   Size:        261     198
 
Bsp1286I G_dGCh'C

Cuts at:      0     140     231     459 
   Size:        140      91     228

  Fragments arranged by size: 

                 228     140      91
 
BsrDI GCAATG_nn'

Cuts at:      0     374     459 
   Size:        374      85
 
BsrGI T'GTAC_A

Cuts at:      0     215     459 
   Size:        215     244
 
BstEII G'GTnAC_C

Cuts at:      0     252     459 
   Size:        252     207
 
BstYI r'GATC_y

Cuts at:      0     113     459 
   Size:        113     346
 
BtgI C'CryG_G

Cuts at:      0     231     383     459 
   Size:        231     152      76

  Fragments arranged by size: 

                 231     152      76
 
BtsI GCAGTG_nn'

Cuts at:      0     273     459 
   Size:        273     186
 
EaeI y'GGCC_r

Cuts at:      0     191     323     459 
   Size:        191     132     136

  Fragments arranged by size: 

                 191     136     132
 
EarI CTCTTCn'nnn_

Cuts at:      0      82     459 
   Size:         82     377
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     334     459 
   Size:        334     125
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     151     459 
   Size:        151     308
 
EcoO109I rG'GnC_Cy

Cuts at:      0     102     318     459 
   Size:        102     216     141

  Fragments arranged by size: 

                 216     141     102
 
HaeII r_GCGC'y

Cuts at:      0     305     459 
   Size:        305     154
 
MscI TGG'CCA

Cuts at:      0     193     325     459 
   Size:        193     132     134

  Fragments arranged by size: 

                 193     134     132
 
MslI CAynn'nnrTG

Cuts at:      0     334     365     459 
   Size:        334      31      94

  Fragments arranged by size: 

                 334      94      31
 
MspA1I CmG'CkG

Cuts at:      0      13     283     459 
   Size:         13     270     176

  Fragments arranged by size: 

                 270     176      13
 
PfoI T'CCnGG_A

Cuts at:      0     311     459 
   Size:        311     148
 
SapI GCTCTTCn'nnn_

Cuts at:      0      82     459 
   Size:         82     377
 
SfcI C'TryA_G

Cuts at:      0     354     459 
   Size:        354     105
 
StyI C'CwwG_G

Cuts at:      0     257     459 
   Size:        257     202
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      39     150     459 
   Size:         39     111     309

  Fragments arranged by size: 

                 309     111      39
 
TatI w'GTAC_w

Cuts at:      0     215     459 
   Size:        215     244
 

 Enzymes that do cut:

     AlfI    BamHI     BanI    BanII     BbsI    BbvCI     BclI Bme1580I
     BmrI     BpmI   Bpu10I    BsaAI    BseRI    BseYI     BsgI Bsp1286I
    BsrDI    BsrGI   BstEII    BstYI     BtgI     BtsI     EaeI     EarI
  Eco57MI    EcoNI EcoO109I    HaeII     MscI     MslI   MspA1I     PfoI
     SapI     SfcI     StyI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BbeI
     BcgI    BciVI    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI
     BplI    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrFI   BssHII    BssSI   BstAPI    BstBI    BstXI  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI
  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI