Restriction map for pF1KB7356
##################################
# NO CUT ENZYMES (   93 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AflII       
AgeI        AhdI        Aor51HI     AscI        Asp718I     
AspEI       AvaI        AviII       AvrII       BamHI       
BanI        BbeI        BcgI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BsaBI       
BsaI        BsiEI       BsiWI       BsmBI       BsoBI       
Bsp1407I    BspLU11I    BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstZ17I     CelII       
Cfr10I      CpoI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoRI       EcoRV       FseI        
FspI        HaeII       HpaI        KasI        KpnI        
KspI        MamI        MluI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PaeR7I      PinAI       PmeI        
PshAI       Psp1406I    PvuI        RsrII       SacII       
SalI        SbfI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SwaI        XbaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccIII       1223  TCCGGA               T^CCGGA
Alw44I       3142  GTGCAC               G^TGCAC
ApaLI        3142  GTGCAC               G^TGCAC
AseI         2584  ATTAAT               AT^TAAT
AsnI         2584  ATTAAT               AT^TAAT
Asp700I       117  GAATGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         2209  GACTGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI        1662  GCTGAGG              CCTCAGC(-5/-2)
BciVI        2641  GGATAC               GTATCC(6/5)
BcnI         1309  CCGGG                CC^[GC]GG
BsaWI        1223  TCCGGA               [AT]^CCGG[AT]
BseAI        1223  TCCGGA               T^CCGGA
BsmAI         422  GAGAC                GTCTC(1/5)
BsmI         2673  GAATGC               GAATGC(1/-1)
BspDI        2905  ATCGAT               AT^CGAT
BspEI        1223  TCCGGA               T^CCGGA
BsrBI        2432  GAGCGG               CCGCTC(-3/-3)
BstAPI       3394  GCAGAAGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        835  GGTGACC              G^GT[ACGT]ACC
BstPI         835  GGTGACC              G^GT[ACGT]ACC
ClaI         2905  ATCGAT               AT^CGAT
DraIII        776  CACATGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I       835  GGTGACC              G^GT[ACGT]ACC
EcoT22I      2113  ATGCAT               ATGCA^T
HincII       1419  GTTGAC               GT[CT]^[GA]AC
HindII       1419  GTTGAC               GT[CT]^[GA]AC
HindIII      1615  AAGCTT               A^AGCTT
MfeI         1580  CAATTG               C^AATTG
MroI         1223  TCCGGA               T^CCGGA
MunI         1580  CAATTG               C^AATTG
NciI         1309  CCGGG                CC^[GC]GG
NsiI         2113  ATGCAT               ATGCA^T
PflFI        2209  GACTGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI        2444  AGGTCCC              [GA]G^G[AT]CC[CT]
PshBI        2584  ATTAAT               AT^TAAT
PvuII        2941  CAGCTG               CAG^CTG
SacI         3468  GAGCTC               GAGCT^C
SapI         1288  GCTCTTC              GCTCTTC(1/4)
SmlI         3341  CTCAAG               C^T[CT][GA]AG
Tth111I      2209  GACTGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmnI          117  GAATGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AccII        1352  CGCG                 CG^CG
             3265  CGCG                
AflIII        136  ACGTGT               A^C[GA][CT]GT
             2242  ACGTGT              
ApaI         1311  GGGCCC               GGGCC^C
             2876  GGGCCC              
BbrPI         135  CACGTG               CAC^GTG
             2241  CACGTG              
BbsI         2866  GAAGAC               GAAGAC(2/6)
              246  GTCTTC              
BclI         1904  TGATCA               T^GATCA
             2310  TGATCA              
BfaI         1966  CTAG                 C^TAG
             3053  CTAG                
BpuAI        2866  GAAGAC               GAAGAC(2/6)
              246  GTCTTC              
BsaAI         135  CACGTG               [CT]AC^GT[GA]
             2241  CACGTG              
BseRI         202  GAGGAG               GAGGAG(10/8)
             3364  GAGGAG              
BspHI          81  TCATGA               T^CATGA
             1883  TCATGA              
BsrDI         934  CATTGC               GCAATG(2/0)
             1937  GCAATG              
BstUI        1352  CGCG                 CG^CG
             3265  CGCG                
BstXI        3212  CCATGGGCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3480  CCAGAAGCCTGG        
CfoI         1351  GCGC                 GCG^C
             2418  GCGC                
DrdI         2206  GACGACTGCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2437  GACGCCAAGGTC        
EcoNI        1278  CCTGAAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1716  CCTGGATGAGG         
FbaI         1904  TGATCA               T^GATCA
             2310  TGATCA              
HhaI         1351  GCGC                 GCG^C
             2418  GCGC                
HinP1I       1351  GCGC                 G^CGC
             2418  GCGC                
MaeI         1966  CTAG                 C^TAG
             3053  CTAG                
MspA1I       1264  CCGCTG               C[AC]G^C[GT]G
             2941  CAGCTG              
MvnI         1352  CGCG                 CG^CG
             3265  CGCG                
NcoI         1913  CCATGG               C^CATGG
             3212  CCATGG              
NspI         1544  GCATGT               [GA]CATG^[CT]
             2303  GCATGT              
PleI         1255  GACTC                GAGTC(4/5)
             3457  GAGTC               
PmaCI         135  CACGTG               CAC^GTG
             2241  CACGTG              
PmlI          135  CACGTG               CAC^GTG
             2241  CACGTG              
RcaI           81  TCATGA               T^CATGA
             1883  TCATGA              
ScaI          427  AGTACT               AGT^ACT
              473  AGTACT              
SspI         1789  AATATT               AAT^ATT
             2097  AATATT              
StuI         1555  AGGCCT               AGG^CCT
             2512  AGGCCT              
XcmI           34  CCAGGTTGCTTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             3480  CCAGAAGCCTGGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   91 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AluI        
AlwI        AlwNI       AocI        ApoI        AspHI       
AvaII       BalI        BanII       BbvI        BmyI        
BpmI        BsaHI       BsaJI       BsgI        BsiHKAI     
BsiYI       BslI        BsmFI       Bsp1286I    BspMI       
BsrI        BssKI       BstF5I      BstNI       BstYI       
Bsu36I      Cac8I       Cfr13I      DdeI        DpnII       
DraI        DraII       DsaI        EaeI        EarI        
Eco81I      EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HgaI        
Hin1I       HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       PflMI       PstI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XhoII       
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