Restriction map for pF1KB7356
 (Linear) (Six-base) MAPSORT of: KIBB7356.seq  Check: 801  from: 1  to: 3531



KIBB7356.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:08  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2053    3531 
   Size:       2053    1478
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40    1213    1300    2241    2551    3531 
   Size:         40    1173      87     941     310     980

  Fragments arranged by size: 

                1173     980     941     310      87      40
 
AflIII A'CryG_T

Cuts at:      0     136    2242    3531 
   Size:        136    2106    1289

  Fragments arranged by size: 

                2106    1289     136
 
AleI CACnn'nnGTG

Cuts at:      0    2245    3531 
   Size:       2245    1286
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1414    3531 
   Size:       1414    2117
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     740     772    3531 
   Size:        740      32    2759

  Fragments arranged by size: 

                2759     740      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      12     264    1191    1686    1703    2946    3531 
   Size:         12     252     927     495      17    1243     585

  Fragments arranged by size: 

                1243     927     585     495     252      17      12
 
ApaI G_GGCC'C

Cuts at:      0    1315    2880    3531 
   Size:       1315    1565     651

  Fragments arranged by size: 

                1565    1315     651
 
ApaLI G'TGCA_C

Cuts at:      0    3142    3531 
   Size:       3142     389
 
ApoI r'AATT_y

Cuts at:      0     370     786    3203    3531 
   Size:        370     416    2417     328

  Fragments arranged by size: 

                2417     416     370     328
 
AseI AT'TA_AT

Cuts at:      0    2585    3531 
   Size:       2585     946
 
BanII G_rGCy'C

Cuts at:      0    1315    1589    2880    2927    3421    3472    3531 
   Size:       1315     274    1291      47     494      51      59

  Fragments arranged by size: 

                1315    1291     494     274      59      51      47
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     239    2873    3531 
   Size:        239    2634     658

  Fragments arranged by size: 

                2634     658     239
 
BbvCI CC'TCA_GC

Cuts at:      0    1663    3531 
   Size:       1663    1868
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2635    3531 
   Size:       2635     896
 
BclI T'GATC_A

Cuts at:      0    1904    2310    3531 
   Size:       1904     406    1221

  Fragments arranged by size: 

                1904    1221     406
 
BfrBI ATG'CAT

Cuts at:      0    2115    3531 
   Size:       2115    1416
 
Bme1580I G_kGCm'C

Cuts at:      0    1315    1495    2880    3146    3531 
   Size:       1315     180    1385     266     385

  Fragments arranged by size: 

                1385    1315     385     266     180
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1159    1536    3374    3531 
   Size:       1159     377    1838     157

  Fragments arranged by size: 

                1838    1159     377     157
 
Bpu10I CC'TnA_GC

Cuts at:      0    1663    3301    3531 
   Size:       1663    1638     230

  Fragments arranged by size: 

                1663    1638     230
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    3326    3531 
   Size:       3326     205
 
BsaAI yAC'GTr

Cuts at:      0     137    2243    3531 
   Size:        137    2106    1288

  Fragments arranged by size: 

                2106    1288     137
 
BsaHI Gr'CG_yC

Cuts at:      0    2292    2411    2438    2870    3385    3531 
   Size:       2292     119      27     432     515     146

  Fragments arranged by size: 

                2292     515     432     146     119      27
 
BsaWI w'CCGG_w

Cuts at:      0    1223    3531 
   Size:       1223    2308
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     196     226    3531 
   Size:        196      30    3305

  Fragments arranged by size: 

                3305     196      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     217    3379    3531 
   Size:        217    3162     152

  Fragments arranged by size: 

                3162     217     152
 
BseYI C'CCAG_C

Cuts at:      0    1315    3422    3531 
   Size:       1315    2107     109

  Fragments arranged by size: 

                2107    1315     109
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     240     369    1799    3461    3531 
   Size:        240     129    1430    1662      70

  Fragments arranged by size: 

                1662    1430     240     129      70
 
BsiHKAI G_wGCw'C

Cuts at:      0    1223    3146    3472    3531 
   Size:       1223    1923     326      59

  Fragments arranged by size: 

                1923    1223     326      59
 
BsmI GAATG_Cn'

Cuts at:      0    2679    3531 
   Size:       2679     852
 
Bsp1286I G_dGCh'C

Cuts at:      0    1223    1315    1495    1589    2880    2927    3146    3421 
   Size:       1223      92     180      94    1291      47     219     275

Cuts at:   3421    3472    3531 
   Size:         51      59

  Fragments arranged by size: 

                1291    1223     275     219     180      94      92      59
                  51      47
 
BspEI T'CCGG_A

Cuts at:      0    1223    3531 
   Size:       1223    2308
 
BspHI T'CATG_A

Cuts at:      0      81    1883    3531 
   Size:         81    1802    1648

  Fragments arranged by size: 

                1802    1648      81
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     450    2053    3024    3531 
   Size:        450    1603     971     507

  Fragments arranged by size: 

                1603     971     507     450
 
BsrBI CCG'CTC

Cuts at:      0    2434    3531 
   Size:       2434    1097
 
BsrDI GCAATG_nn'

Cuts at:      0     933    1944    3531 
   Size:        933    1011    1587

  Fragments arranged by size: 

                1587    1011     933
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    3400    3531 
   Size:       3400     131
 
BstEII G'GTnAC_C

Cuts at:      0     835    3531 
   Size:        835    2696
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    3219    3487    3531 
   Size:       3219     268      44

  Fragments arranged by size: 

                3219     268      44
 
BstYI r'GATC_y

Cuts at:      0      30    2561    2715    3531 
   Size:         30    2531     154     816

  Fragments arranged by size: 

                2531     816     154      30
 
Bsu36I CC'TnA_GG

Cuts at:      0     841    1696    3428    3531 
   Size:        841     855    1732     103

  Fragments arranged by size: 

                1732     855     841     103
 
BtgI C'CryG_G

Cuts at:      0    1211    1913    3212    3531 
   Size:       1211     702    1299     319

  Fragments arranged by size: 

                1299    1211     702     319
 
ClaI AT'CG_AT

Cuts at:      0    2906    3531 
   Size:       2906     625
 
DraI TTT'AAA

Cuts at:      0     947    1136    1782    3167    3531 
   Size:        947     189     646    1385     364

  Fragments arranged by size: 

                1385     947     646     364     189
 
DraIII CAC_nnn'GTG

Cuts at:      0     781    3531 
   Size:        781    2750
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2212    2443    3531 
   Size:       2212     231    1088

  Fragments arranged by size: 

                2212    1088     231
 
EaeI y'GGCC_r

Cuts at:      0     730     985    2225    2691    3531 
   Size:        730     255    1240     466     840

  Fragments arranged by size: 

                1240     840     730     466     255
 
EarI CTCTTCn'nnn_

Cuts at:      0    1295    1565    1913    3531 
   Size:       1295     270     348    1618

  Fragments arranged by size: 

                1618    1295     348     270
 
EcoICRI GAG'CTC

Cuts at:      0    3470    3531 
   Size:       3470      61
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40    1159    1213    1300    1536    2241    2551    3374 
   Size:         40    1119      54      87     236     705     310     823

Cuts at:   3374    3531 
   Size:        157

  Fragments arranged by size: 

                1119     823     705     310     236     157      87      54
                  40
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1282    1720    3531 
   Size:       1282     438    1811

  Fragments arranged by size: 

                1811    1282     438
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1197    1312    2445    2877    3531 
   Size:       1197     115    1133     432     654

  Fragments arranged by size: 

                1197    1133     654     432     115
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1260    1412    3531 
   Size:       1260     152    2119

  Fragments arranged by size: 

                2119    1260     152
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      15      47    1150    1182    3343    3375    3531 
   Size:         15      32    1103      32    2161      32     156

  Fragments arranged by size: 

                2161    1103     156      32      32      32      15
 
HincII GTy'rAC

Cuts at:      0    1421    3531 
   Size:       1421    2110
 
HindIII A'AGCT_T

Cuts at:      0    1615    3531 
   Size:       1615    1916
 
MfeI C'AATT_G

Cuts at:      0    1580    3531 
   Size:       1580    1951
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1818    1968    2828    3057    3531 
   Size:       1818     150     860     229     474

  Fragments arranged by size: 

                1818     860     474     229     150
 
MscI TGG'CCA

Cuts at:      0     732     987    2227    3531 
   Size:        732     255    1240    1304

  Fragments arranged by size: 

                1304    1240     732     255
 
MslI CAynn'nnrTG

Cuts at:      0    1912    2245    3217    3531 
   Size:       1912     333     972     314

  Fragments arranged by size: 

                1912     972     333     314
 
MspA1I CmG'CkG

Cuts at:      0    1266    2943    3531 
   Size:       1266    1677     588

  Fragments arranged by size: 

                1677    1266     588
 
NcoI C'CATG_G

Cuts at:      0    1913    3212    3531 
   Size:       1913    1299     319

  Fragments arranged by size: 

                1913    1299     319
 
NsiI A_TGCA'T

Cuts at:      0    2117    3531 
   Size:       2117    1414
 
NspI r_CATG'y

Cuts at:      0    1548    2307    3531 
   Size:       1548     759    1224

  Fragments arranged by size: 

                1548    1224     759
 
PasI CC'CwG_GG

Cuts at:      0    1495    3531 
   Size:       1495    2036
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      12     264     452    2406    3531 
   Size:         12     252     188    1954    1125

  Fragments arranged by size: 

                1954    1125     252     188      12
 
PfoI T'CCnGG_A

Cuts at:      0    1715    3531 
   Size:       1715    1816
 
PmlI CAC'GTG

Cuts at:      0     137    2243    3531 
   Size:        137    2106    1288

  Fragments arranged by size: 

                2106    1288     137
 
PpuMI rG'GwC_Cy

Cuts at:      0    2445    3531 
   Size:       2445    1086
 
PsiI TTA'TAA

Cuts at:      0      55    3188    3531 
   Size:         55    3133     343

  Fragments arranged by size: 

                3133     343      55
 
PspOMI G'GGCC_C

Cuts at:      0    1311    2876    3531 
   Size:       1311    1565     655

  Fragments arranged by size: 

                1565    1311     655
 
PstI C_TGCA'G

Cuts at:      0    1130    1205    2343    3416    3531 
   Size:       1130      75    1138    1073     115

  Fragments arranged by size: 

                1138    1130    1073     115      75
 
PvuII CAG'CTG

Cuts at:      0    2943    3531 
   Size:       2943     588
 
SacI G_AGCT'C

Cuts at:      0    3472    3531 
   Size:       3472      59
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1295    3531 
   Size:       1295    2236
 
ScaI AGT'ACT

Cuts at:      0     429     475    3531 
   Size:        429      46    3056

  Fragments arranged by size: 

                3056     429      46
 
SfcI C'TryA_G

Cuts at:      0     357    1126    1201    2051    2339    3003    3412    3531 
   Size:        357     769      75     850     288     664     409     119

  Fragments arranged by size: 

                 850     769     664     409     357     288     119      75
            
 
SmlI C'TyrA_G

Cuts at:      0    3341    3531 
   Size:       3341     190
 
SspI AAT'ATT

Cuts at:      0    1791    2099    3531 
   Size:       1791     308    1432

  Fragments arranged by size: 

                1791    1432     308
 
StuI AGG'CCT

Cuts at:      0    1557    2514    3531 
   Size:       1557     957    1017

  Fragments arranged by size: 

                1557    1017     957
 
StyI C'CwwG_G

Cuts at:      0    1621    1913    2441    3212    3531 
   Size:       1621     292     528     771     319

  Fragments arranged by size: 

                1621     771     528     319     292
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1487    2588    3531 
   Size:       1487    1101     943

  Fragments arranged by size: 

                1487    1101     943
 
TatI w'GTAC_w

Cuts at:      0     380     427     473     980    1953    3531 
   Size:        380      47      46     507     973    1578

  Fragments arranged by size: 

                1578     973     507     380      47      46
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     196     228    3531 
   Size:        196      32    3303

  Fragments arranged by size: 

                3303     196      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2212    3531 
   Size:       2212    1319
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      41    3487    3531 
   Size:         41    3446      44

  Fragments arranged by size: 

                3446      44      41
 
XmnI GAAnn'nnTTC

Cuts at:      0     121    3531 
   Size:        121    3410
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AseI    BanII     BbsI    BbvCI    BciVI     BclI
    BfrBI Bme1580I     BpmI   Bpu10I    BpuEI    BsaAI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI   BstAPI   BstEII    BstXI    BstYI
   Bsu36I     BtgI     ClaI     DraI   DraIII     DrdI     EaeI     EarI
  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI    Hin4I   HincII  HindIII
     MfeI     MmeI     MscI     MslI   MspA1I     NcoI     NsiI     NspI
     PasI    PflMI     PfoI     PmlI    PpuMI     PsiI   PspOMI     PstI
    PvuII     SacI     SapI     ScaI     SfcI     SmlI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
     BcgI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI
     BsaI    BsaBI    BsiEI    BsiWI    BsmBI    BsrFI    BsrGI   BssHII
    BssSI    BstBI  BstZ17I    BtgZI     BtsI    CspCI     EagI     EciI
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII     HpaI     KasI
     KpnI     MluI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     PacI     PciI     PmeI     PpiI    PshAI    PspXI     PsrI
     PvuI    RsrII    SacII     SalI    SanDI     SbfI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SwaI     XbaI     XhoI     XmaI     ZraI
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