Restriction map for pF1KE1731
##################################
# NO CUT ENZYMES (  130 enzymes) #
##################################

AatII       AccIII      AclI        AcsI        AflII       
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     AspEI       AvrII       BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BfrI        BglII       BlnI        BpuAI       BsaBI       
BseAI       BseRI       BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrDI       BsrGI       BssHII      Bst1107I    
BstAPI      BstBI       BstEII      BstF5I      BstPI       
BstXI       BstZ17I     Bsu36I      ClaI        CpoI        
DraI        DraIII      DrdI        Eam1105I    Eco47III    
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FokI        HindIII     HpaI        
HphI        KspI        MaeIII      MamI        MfeI        
MluI        MroI        MseI        MslI        MunI        
NcoI        NdeI        NheI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfuI        
SgrAI       SmaI        SmlI        SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TfiI        Tru9I       Tsp45I      Tsp509I     XbaI        
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

Acc65I        284  GGTACC               G^GTACC
AccI          103  GTCTAC               GT^[AC][GT]AC
AflIII        461  ACGTGT               A^C[GA][CT]GT
Asp718I       284  GGTACC               G^GTACC
AspI          405  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          177  CCCGAG               C^[CT]CG[GA]G
AviII         711  TGCGCA               TGC^GCA
BamHI         359  GGATCC               G^GATCC
BfaI          413  CTAG                 C^TAG
BlpI           24  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I       24  GCTCAGC              GC^T[ACGT]AGC
BsaI          268  GGTCTC               GGTCTC(1/5)
BsaWI         401  ACCGGA               [AT]^CCGG[AT]
BsgI          172  CTGCAC               GTGCAG(16/14)
BsoBI         177  CCCGAG               C^[CT]CG[GA]G
BstYI         359  GGATCC               [GA]^GATC[CT]
CelII          24  GCTCAGC              GC^T[ACGT]AGC
DdeI           25  CTCAG                C^T[ACGT]AG
DpnII         360  GATC                 ^GATC
DsaI           93  CCGTGG               C^C[GA][CT]GG
EarI          271  CTCTTC               CTCTTC(1/4)
EcoT14I       114  CCTTGG               C^C[AT][AT]GG
FseI          481  GGCCGGCC             GGCCGG^CC
FspI          711  TGCGCA               TGC^GCA
HincII        190  GTCAAC               GT[CT]^[GA]AC
HindII        190  GTCAAC               GT[CT]^[GA]AC
HinfI         311  GACTC                G^A[ACGT]TC
KpnI          284  GGTACC               GGTAC^C
Ksp632I       271  CTCTTC               CTCTTC(1/4)
MaeI          413  CTAG                 C^TAG
MboI          360  GATC                 ^GATC
MboII         272  TCTTC                GAAGA(8/7)
MflI          359  GGATCC               [GA]^GATC[CT]
NdeII         360  GATC                 ^GATC
NlaIII        294  CATG                 CATG^
NotI          182  GCGGCCGC             GC^GGCCGC
PflFI         405  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI          311  GACTC                GAGTC(4/5)
Sau3AI        360  GATC                 ^GATC
SfaNI          82  GATGC                GCATC(5/9)
SnaBI         381  TACGTA               TAC^GTA
StyI          114  CCTTGG               C^C[AT][AT]GG
TaqI          682  TCGA                 T^CGA
Tth111I       405  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       682  TCGA                 T^CGA
XhoII         359  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AlwI          360  GATCC                GGATC(4/5)
              359  GGATC               
ApaI           57  GGGCCC               GGGCC^C
              425  GGGCCC              
AspHI         109  GAGCAC               G[AT]GC[AT]^C
              325  GAGCAC              
BalI          257  TGGCCA               TGG^CCA
              290  TGGCCA              
BbeI          123  GGCGCC               GGCGC^C
              131  GGCGCC              
BsaAI         381  TACGTA               [CT]AC^GT[GA]
              460  TACGTG              
BsiEI          90  CGGCCG               CG[GA][CT]^CG
              183  CGGCCG              
BsiHKAI       109  GAGCAC               G[AT]GC[AT]^C
              325  GAGCAC              
BsmFI         306  GGGAC                GGGAC(10/14)
              557  GGGAC               
BsrBI         180  GAGCGG               CCGCTC(-3/-3)
              355  GAGCGG              
BsrFI         431  GCCGGC               [GA]^CCGG[CT]
              482  GCCGGC              
BsrI          687  ACTGG                ACTGG(1/-1)
              707  CCAGT               
BssSI         364  CACGAG               CACGAG(-5/-1)
              562  CACGAG              
Cfr10I        431  GCCGGC               [GA]^CCGG[CT]
              482  GCCGGC              
DraII          57  GGGCCCT              [GA]G^G[ACGT]CC[CT]
              424  GGGGCCC             
EagI           90  CGGCCG               C^GGCCG
              183  CGGCCG              
EclXI          90  CGGCCG               C^GGCCG
              183  CGGCCG              
Eco52I         90  CGGCCG               C^GGCCG
              183  CGGCCG              
EcoO109I       57  GGGCCCT              [GA]G^G[ACGT]CC[CT]
              424  GGGGCCC             
HgaI          101  GCGTC                GACGC(5/10)
              188  GCGTC               
KasI          123  GGCGCC               G^GCGCC
              131  GGCGCC              
MluNI         257  TGGCCA               TGG^CCA
              290  TGGCCA              
MscI          257  TGGCCA               TGG^CCA
              290  TGGCCA              
NaeI          431  GCCGGC               GCC^GGC
              482  GCCGGC              
NarI          123  GGCGCC               GG^CGCC
              131  GGCGCC              
NgoMIV        431  GCCGGC               G^CCGGC
              482  GCCGGC              
PflMI         363  CCACGAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              561  CCACGAGATGG         
PvuII         169  CAGCTG               CAG^CTG
              697  CAGCTG              
SfoI          123  GGCGCC               GGC^GCC
              131  GGCGCC              
Van91I        363  CCACGAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              561  CCACGAGATGG         


#########################################
# 3 OR MORE CUT ENZYMES (   52 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AvaII       BanI        BanII       BbvI        BcnI        
BglI        BmyI        BpmI        BsaHI       BsaJI       
BsiYI       BslI        BsmAI       Bsp1286I    BssKI       
BstNI       BstUI       Cac8I       CfoI        Cfr13I      
EaeI        EcoRII      FauI        Fnu4HI      HaeII       
HaeIII      HapII       HhaI        Hin1I       HinP1I      
HpaII       ItaI        MaeII       MnlI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NlaIV       RsaI        Sau96I      ScrFI       SfcI        
TseI        TspRI       
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