Restriction map for pF1KB7334
 (Linear) (Six-base) MAPSORT of: KIBB7334.seq  Check: 7049  from: 1  to: 4323



KIBB7334.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:08  ..

AatII G_ACGT'C

Cuts at:      0     173    4323 
   Size:        173    4150
 
AccI GT'mk_AC

Cuts at:      0    1991    3438    4323 
   Size:       1991    1447     885

  Fragments arranged by size: 

                1991    1447     885
 
Acc65I G'GTAC_C

Cuts at:      0     491    4323 
   Size:        491    3832
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1124    2156    2603    2990    3555    3794    4323 
   Size:       1124    1032     447     387     565     239     529

  Fragments arranged by size: 

                1124    1032     565     529     447     387     239
 
AfeI AGC'GCT

Cuts at:      0      94    4323 
   Size:         94    4229
 
AflIII A'CryG_T

Cuts at:      0    2548    4323 
   Size:       2548    1775
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3499    4323 
   Size:       3499     824
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2005    2037    4323 
   Size:       2005      32    2286

  Fragments arranged by size: 

                2286    2005      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     302    3244    4323 
   Size:        302    2942    1079

  Fragments arranged by size: 

                2942    1079     302
 
ApoI r'AATT_y

Cuts at:      0     406    1054    1359    1470    2113    2462    2490    3322 
   Size:        406     648     305     111     643     349      28     832

Cuts at:   3322    4323 
   Size:       1001

  Fragments arranged by size: 

                1001     832     648     643     406     349     305     111
                  28
 
AseI AT'TA_AT

Cuts at:      0     422    4323 
   Size:        422    3901
 
AvaI C'yCGr_G

Cuts at:      0      48     671    4323 
   Size:         48     623    3652

  Fragments arranged by size: 

                3652     623      48
 
AvrII C'CTAG_G

Cuts at:      0    3979    4323 
   Size:       3979     344
 
BanI G'GyrC_C

Cuts at:      0     156     491    4323 
   Size:        156     335    3832

  Fragments arranged by size: 

                3832     335     156
 
BanII G_rGCy'C

Cuts at:      0     209    4078    4126    4323 
   Size:        209    3869      48     197

  Fragments arranged by size: 

                3869     209     197      48
 
BbeI G_GCGC'C

Cuts at:      0     160    4323 
   Size:        160    4163
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2287    3662    3732    4323 
   Size:       2287    1375      70     591

  Fragments arranged by size: 

                2287    1375     591      70
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2737    4323 
   Size:       2737    1586
 
BclI T'GATC_A

Cuts at:      0     272     543    2777    4323 
   Size:        272     271    2234    1546

  Fragments arranged by size: 

                2234    1546     272     271
 
BfrBI ATG'CAT

Cuts at:      0     569    4323 
   Size:        569    3754
 
BglI GCCn_nnn'nGGC

Cuts at:      0    3468    4323 
   Size:       3468     855
 
BglII A'GATC_T

Cuts at:      0    1644    2329    4323 
   Size:       1644     685    1994

  Fragments arranged by size: 

                1994    1644     685
 
Bme1580I G_kGCm'C

Cuts at:      0      61    4323 
   Size:         61    4262
 
BmgBI CAC'GTC

Cuts at:      0    3694    4323 
   Size:       3694     629
 
BmtI G_CTAG'C

Cuts at:      0    3790    4323 
   Size:       3790     533
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2190    4323 
   Size:       2190    2133
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     160     436    1586    4323 
   Size:        160     276    1150    2737

  Fragments arranged by size: 

                2737    1150     276     160
 
Bpu10I CC'TnA_GC

Cuts at:      0     134    2708    4323 
   Size:        134    2574    1615

  Fragments arranged by size: 

                2574    1615     134
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1525    3329    4323 
   Size:       1525    1804     994

  Fragments arranged by size: 

                1804    1525     994
 
BsaAI yAC'GTr

Cuts at:      0    2551    4323 
   Size:       2551    1772
 
BsaBI GATnn'nnATC

Cuts at:      0     398     506    2779    3953    4323 
   Size:        398     108    2273    1174     370

  Fragments arranged by size: 

                2273    1174     398     370     108
 
BsaHI Gr'CG_yC

Cuts at:      0     157     170    4323 
   Size:        157      13    4153

  Fragments arranged by size: 

                4153     157      13
 
BsaWI w'CCGG_w

Cuts at:      0    3902    4022    4323 
   Size:       3902     120     301

  Fragments arranged by size: 

                3902     301     120
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    3119    3149    4323 
   Size:       3119      30    1174

  Fragments arranged by size: 

                3119    1174      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      26     442     712     781    2089    2311    2641    2759 
   Size:         26     416     270      69    1308     222     330     118

Cuts at:   2759    3143    3146    3544    3556    3871    3874    3877    3934 
   Size:        384       3     398      12     315       3       3      57

Cuts at:   3934    3937    4273    4323 
   Size:          3     336      50

  Fragments arranged by size: 

                1308     416     398     384     336     330     315     270
                 222     118      69      57      50      26      12       3
                   3       3       3
 
BseYI C'CCAG_C

Cuts at:      0     682    4323 
   Size:        682    3641
 
BsiHKAI G_wGCw'C

Cuts at:      0     209    4009    4251    4323 
   Size:        209    3800     242      72

  Fragments arranged by size: 

                3800     242     209      72
 
BsmI GAATG_Cn'

Cuts at:      0     355    1951    4323 
   Size:        355    1596    2372

  Fragments arranged by size: 

                2372    1596     355
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     454    3904    4323 
   Size:        454    3450     419

  Fragments arranged by size: 

                3450     454     419
 
Bsp1286I G_dGCh'C

Cuts at:      0      61     209    4009    4078    4126    4251    4323 
   Size:         61     148    3800      69      48     125      72

  Fragments arranged by size: 

                3800     148     125      72      69      61      48
 
BspHI T'CATG_A

Cuts at:      0    1305    4323 
   Size:       1305    3018
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     122    4323 
   Size:        122    4201
 
BsrBI CCG'CTC

Cuts at:      0     144    4323 
   Size:        144    4179
 
BsrDI GCAATG_nn'

Cuts at:      0    2580    4323 
   Size:       2580    1743
 
BsrFI r'CCGG_y

Cuts at:      0    3263    4323 
   Size:       3263    1060
 
BsrGI T'GTAC_A

Cuts at:      0     524    4323 
   Size:        524    3799
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    3244    4323 
   Size:       3244    1079
 
BstBI TT'CG_AA

Cuts at:      0    3813    4323 
   Size:       3813     510
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     460    2065    4323 
   Size:        460    1605    2258

  Fragments arranged by size: 

                2258    1605     460
 
BstYI r'GATC_y

Cuts at:      0     993    1280    1562    1644    2329    3340    3393    4323 
   Size:        993     287     282      82     685    1011      53     930

  Fragments arranged by size: 

                1011     993     930     685     287     282      82      53
            
 
Bsu36I CC'TnA_GG

Cuts at:      0    1868    4323 
   Size:       1868    2455
 
BtsI GCAGTG_nn'

Cuts at:      0    1292    2147    2351    2604    4323 
   Size:       1292     855     204     253    1719

  Fragments arranged by size: 

                1719    1292     855     253     204
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    2670    2705    4323 
   Size:       2670      35    1618

  Fragments arranged by size: 

                2670    1618      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1192    4323 
   Size:       1192    3131
 
EaeI y'GGCC_r

Cuts at:      0    1971    3138    3460    4323 
   Size:       1971    1167     322     863

  Fragments arranged by size: 

                1971    1167     863     322
 
EarI CTCTTCn'nnn_

Cuts at:      0     561     672     809    1259    1400    1656    1694    2288 
   Size:        561     111     137     450     141     256      38     594

Cuts at:   2288    2420    2618    3302    3521    3676    3743    3863    4037 
   Size:        132     198     684     219     155      67     120     174

Cuts at:   4037    4250    4323 
   Size:        213      73

  Fragments arranged by size: 

                 684     594     561     450     256     219     213     198
                 174     155     141     137     132     120     111      73
                  67      38
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    3386    4323 
   Size:       3386     937
 
EcoICRI GAG'CTC

Cuts at:      0     207    4323 
   Size:        207    4116
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     160     436    1124    1586    2156    2603    2990    3555 
   Size:        160     276     688     462     570     447     387     565

Cuts at:   3555    3794    4323 
   Size:        239     529

  Fragments arranged by size: 

                 688     570     565     529     462     447     387     276
                 239     160
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2008    2178    4323 
   Size:       2008     170    2145

  Fragments arranged by size: 

                2145    2008     170
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1201    3984    4323 
   Size:       1201    2783     339

  Fragments arranged by size: 

                2783    1201     339
 
EcoRI G'AATT_C

Cuts at:      0    3322    4323 
   Size:       3322    1001
 
EcoRV GAT'ATC

Cuts at:      0    2216    3153    4323 
   Size:       2216     937    1170

  Fragments arranged by size: 

                2216    1170     937
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    4288    4323 
   Size:       4288      35
 
HaeII r_GCGC'y

Cuts at:      0      96     160    1734    4323 
   Size:         96      64    1574    2589

  Fragments arranged by size: 

                2589    1574      96      64
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      21      53    1554    1586    2205    2237    2361    2393 
   Size:         21      32    1501      32     619      32     124      32

Cuts at:   2393    2526    2558    3385    3417    3520    3552    4323 
   Size:        133      32     827      32     103      32     771

  Fragments arranged by size: 

                1501     827     771     619     133     124     103      32
                  32      32      32      32      32      32      21
 
HincII GTy'rAC

Cuts at:      0    2432    3035    4323 
   Size:       2432     603    1288

  Fragments arranged by size: 

                2432    1288     603
 
KasI G'GCGC_C

Cuts at:      0     156    4323 
   Size:        156    4167
 
KpnI G_GTAC'C

Cuts at:      0     495    4323 
   Size:        495    3828
 
MfeI C'AATT_G

Cuts at:      0     458     496    3086    4323 
   Size:        458      38    2590    1237

  Fragments arranged by size: 

                2590    1237     458      38
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1249    2203    4323 
   Size:       1249     954    2120

  Fragments arranged by size: 

                2120    1249     954
 
MscI TGG'CCA

Cuts at:      0    1973    4323 
   Size:       1973    2350
 
MslI CAynn'nnrTG

Cuts at:      0     564    3947    4323 
   Size:        564    3383     376

  Fragments arranged by size: 

                3383     564     376
 
MspA1I CmG'CkG

Cuts at:      0      19     105    1320    1876    3241    4323 
   Size:         19      86    1215     556    1365    1082

  Fragments arranged by size: 

                1365    1215    1082     556      86      19
 
NaeI GCC'GGC

Cuts at:      0    3265    4323 
   Size:       3265    1058
 
NarI GG'CG_CC

Cuts at:      0     157    4323 
   Size:        157    4166
 
NgoMIV G'CCGG_C

Cuts at:      0    3263    4323 
   Size:       3263    1060
 
NheI G'CTAG_C

Cuts at:      0    3786    4323 
   Size:       3786     537
 
NsiI A_TGCA'T

Cuts at:      0     571    4323 
   Size:        571    3752
 
PmlI CAC'GTG

Cuts at:      0    2551    4323 
   Size:       2551    1772
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     112     144    4323 
   Size:        112      32    4179

  Fragments arranged by size: 

                4179     112      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1201    3984    4323 
   Size:       1201    2783     339

  Fragments arranged by size: 

                2783    1201     339
 
PstI C_TGCA'G

Cuts at:      0     155     252    2616    4323 
   Size:        155      97    2364    1707

  Fragments arranged by size: 

                2364    1707     155      97
 
PvuII CAG'CTG

Cuts at:      0    1320    1876    3241    4323 
   Size:       1320     556    1365    1082

  Fragments arranged by size: 

                1365    1320    1082     556
 
SacI G_AGCT'C

Cuts at:      0     209    4323 
   Size:        209    4114
 
SapI GCTCTTCn'nnn_

Cuts at:      0     561    4323 
   Size:        561    3762
 
ScaI AGT'ACT

Cuts at:      0    1839    1962    4323 
   Size:       1839     123    2361

  Fragments arranged by size: 

                2361    1839     123
 
SfcI C'TryA_G

Cuts at:      0     151     248     797    1413    2612    3060    4086    4323 
   Size:        151      97     549     616    1199     448    1026     237

  Fragments arranged by size: 

                1199    1026     616     549     448     237     151      97
            
 
SfoI GGC'GCC

Cuts at:      0     158    4323 
   Size:        158    4165
 
SgrAI Cr'CCGG_yG

Cuts at:      0    3263    4323 
   Size:       3263    1060
 
SmaI CCC'GGG

Cuts at:      0      50    4323 
   Size:         50    4273
 
SmlI C'TyrA_G

Cuts at:      0    1504    3344    4323 
   Size:       1504    1840     979

  Fragments arranged by size: 

                1840    1504     979
 
SspI AAT'ATT

Cuts at:      0     268     417    1068    2566    3511    4323 
   Size:        268     149     651    1498     945     812

  Fragments arranged by size: 

                1498     945     812     651     268     149
 
StyI C'CwwG_G

Cuts at:      0    3979    4181    4323 
   Size:       3979     202     142

  Fragments arranged by size: 

                3979     202     142
 
TatI w'GTAC_w

Cuts at:      0     485     524     633    1837    1954    1960    3714    4323 
   Size:        485      39     109    1204     117       6    1754     609

  Fragments arranged by size: 

                1754    1204     609     485     117     109      39       6
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    4017    4049    4215    4247    4323 
   Size:       4017      32     166      32      76

  Fragments arranged by size: 

                4017     166      76      32      32
 
XbaI T'CTAG_A

Cuts at:      0     810    1842    2256    2344    4323 
   Size:        810    1032     414      88    1979

  Fragments arranged by size: 

                1979    1032     810     414      88
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1014    2358    2792    4323 
   Size:       1014    1344     434    1531

  Fragments arranged by size: 

                1531    1344    1014     434
 
XmaI C'CCGG_G

Cuts at:      0      48    4323 
   Size:         48    4275
 
XmnI GAAnn'nnTTC

Cuts at:      0    1826    3729    4323 
   Size:       1826    1903     594

  Fragments arranged by size: 

                1903    1826     594
 
ZraI GAC'GTC

Cuts at:      0     171    4323 
   Size:        171    4152
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AcuI     AfeI   AflIII     AlfI     AloI
    AlwNI     ApoI     AseI     AvaI    AvrII     BanI    BanII     BbeI
     BbsI    BciVI     BclI    BfrBI     BglI    BglII Bme1580I    BmgBI
     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BstAPI    BstBI
    BstXI    BstYI   Bsu36I     BtsI    CspCI     DrdI     EaeI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
    HaeII    Hin4I   HincII     KasI     KpnI     MfeI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI   NgoMIV     NheI     NsiI     PmlI
     PpiI    PpuMI     PstI    PvuII     SacI     SapI     ScaI     SfcI
     SfoI    SgrAI     SmaI     SmlI     SspI     StyI     TatI     TstI
     XbaI     XcmI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AclI    AflII     AgeI     AhdI     AleI     ApaI    ApaLI
     AscI    AsiSI     BaeI    BamHI    BbvCI     BcgI     BlpI     BmrI
     BsaI     BsgI    BsiEI    BsiWI    BspEI   BssHII    BssSI   BstEII
  BstZ17I     BtgI    BtgZI     ClaI     DraI   DraIII     EagI     FseI
     FspI    FspAI  HindIII     HpaI     MluI     NcoI     NdeI     NotI
     NruI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII
     SalI    SanDI     SbfI    SexAI     SfiI     SgfI    SnaBI     SpeI
     SphI     SrfI     StuI     SwaI    TaqII  Tth111I     XhoI
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