Restriction map for pF1KB7310
##################################
# NO CUT ENZYMES (   83 enzymes) #
##################################

AatII       AccIII      AclI        AflII       AflIII      
AgeI        Alw44I      ApaLI       AscI        AseI        
AsnI        AviII       AvrII       BbrPI       BcgI        
BciVI       BclI        BfrI        BglII       BlnI        
BsaAI       BsaBI       BseAI       BsiEI       Bsp1407I    
BspDI       BspEI       BspLU11I    BsrGI       BssHII      
Bst1107I    BstZ17I     ClaI        DraI        EagI        
EclXI       Eco52I      EcoRI       EcoRV       EcoT22I     
FbaI        FspI        HincII      HindII      HindIII     
HpaI        KspI        MamI        MfeI        MluI        
MroI        MseI        MunI        NdeI        NheI        
NotI        NruI        NsiI        PacI        PaeR7I      
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        SacII       SalI        
SbfI        ScaI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
Tru9I       XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

Acc65I       3052  GGTACC               G^GTACC
AcsI         3802  AAATTC               [GA]^AATT[CT]
AhdI         2191  GACAAGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         4099  CCTGAGG              CC^T[ACGT]AGG
Aor51HI      4070  AGCGCT               AGC^GCT
ApoI         3802  AAATTC               [GA]^AATT[CT]
Asp700I       612  GAATGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      3052  GGTACC               G^GTACC
AspEI        2191  GACAAGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          510  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbeI         2265  GGCGCC               GGCGC^C
BsaWI         680  ACCGGA               [AT]^CCGG[AT]
BsiWI        3092  CGTACG               C^GTACG
BsrFI        2812  GCCGGC               [GA]^CCGG[CT]
BstBI        4063  TTCGAA               TT^CGAA
Bsu36I       4099  CCTGAGG              CC^T[ACGT]AGG
Cfr10I       2812  GCCGGC               [GA]^CCGG[CT]
CpoI          682  CGGACCG              CG^G[AT]CCG
Eam1105I     2191  GACAAGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     4070  AGCGCT               AGC^GCT
Eco81I       4099  CCTGAGG              CC^T[ACGT]AGG
FseI         2811  GGCCGGCC             GGCCGG^CC
KasI         2265  GGCGCC               G^GCGCC
KpnI         3052  GGTACC               GGTAC^C
NaeI         2812  GCCGGC               GCC^GGC
NarI         2265  GGCGCC               GG^CGCC
NgoMIV       2812  GCCGGC               G^CCGGC
NspV         4063  TTCGAA               TT^CGAA
PflFI         510  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
RsrII         682  CGGACCG              CG^G[AT]CCG
SapI         4066  GAAGAGC              GCTCTTC(1/4)
SfoI         2265  GGCGCC               GGC^GCC
SfuI         4063  TTCGAA               TT^CGAA
StuI          389  AGGCCT               AGG^CCT
TaqI         4064  TCGA                 T^CGA
Tsp509I      3803  AATT                 ^AATT
Tth111I       510  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I      4064  TCGA                 T^CGA
XmnI          612  GAATGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AccI         1102  GTAGAC               GT^[AC][GT]AC
             3355  GTCTAC              
AcyI         2094  GGCGTC               G[GA]^CG[CT]C
             2265  GGCGCC              
BamHI        1554  GGATCC               G^GATCC
             1779  GGATCC              
BbsI         4017  GAAGAC               GAAGAC(2/6)
             4244  GAAGAC              
BlpI         1043  GCTCAGC              GC^T[ACGT]AGC
             2738  GCTCAGC             
Bpu1102I     1043  GCTCAGC              GC^T[ACGT]AGC
             2738  GCTCAGC             
BpuAI        4017  GAAGAC               GAAGAC(2/6)
             4244  GAAGAC              
BsaHI        2094  GGCGTC               G[GA]^CG[CT]C
             2265  GGCGCC              
BsmI          612  GAATGC               GAATGC(1/-1)
             3557  GCATTC              
BspHI         243  TCATGA               T^CATGA
              620  TCATGA              
BsrDI        1356  CATTGC               GCAATG(2/0)
             2340  CATTGC              
BssSI         849  CACGAG               CACGAG(-5/-1)
             3102  CACGAG              
BstEII       1410  GGTGACC              G^GT[ACGT]ACC
             2013  GGTCACC             
BstPI        1410  GGTGACC              G^GT[ACGT]ACC
             2013  GGTCACC             
CelII        1043  GCTCAGC              GC^T[ACGT]AGC
             2738  GCTCAGC             
DrdI         3823  GACTGGCGCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4036  GACAACAGGGTC        
EarI         4066  GAAGAG               CTCTTC(1/4)
             4176  GAAGAG              
EcoNI         528  CCTACCCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2445  CCTGGGCAAGG         
EcoO65I      1410  GGTGACC              G^GT[ACGT]ACC
             2013  GGTCACC             
Hin1I        2094  GGCGTC               G[GA]^CG[CT]C
             2265  GGCGCC              
Ksp632I      4066  GAAGAG               CTCTTC(1/4)
             4176  GAAGAG              
MaeII         504  ACGT                 A^CGT
             4343  ACGT                
RcaI          243  TCATGA               T^CATGA
              620  TCATGA              
SexAI         255  ACCAGGT              A^CC[AT]GGT
             2436  ACCAGGT             
SfiI         1965  GGCCTATGGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             3495  GGCCCCCAAGGCC       
SmlI         1393  CTCAAG               C^T[CT][GA]AG
             3667  CTTGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  110 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       ApaI        AspHI       AvaI        AvaII       
BalI        BanI        BanII       BbvCI       BbvI        
BcnI        BfaI        BglI        BmyI        BpmI        
BsaI        BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmBI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrBI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       BstUI       
BstXI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DraIII      DsaI        
EaeI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        MaeI        MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NcoI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        PpuMI       
PstI        PvuII       RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmaI        
StyI        TfiI        TseI        Tsp45I      TspRI       
Van91I      XcmI        XhoII       XmaCI       XmaI        
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