Restriction map for pF1KB7278
##################################
# NO CUT ENZYMES (  100 enzymes) #
##################################

AatII       Acc65I      AccIII      AflII       AflIII      
AhdI        AocI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AviII       
AvrII       BamHI       BbrPI       BbsI        BclI        
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BseAI       BsgI        
BsiWI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrGI       BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DraI        DraIII      DrdI        
Eam1105I    Eco81I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HincII      
HindII      HindIII     HpaI        KpnI        MamI        
MfeI        MluI        MroI        MseI        MunI        
NcoI        NheI        NruI        NsiI        NspV        
PacI        PflFI       PmaCI       PmeI        PmlI        
PshAI       PshBI       PvuI        RcaI        RsrII       
SalI        ScaI        SfiI        SfuI        SnaBI       
SphI        SspBI       SspI        StuI        SwaI        
TfiI        Tru9I       TspRI       Tth111I     XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AclI          485  AACGTT               AA^CGTT
AcsI          943  AAATTC               [GA]^AATT[CT]
AgeI         1287  ACCGGT               A^CCGGT
Alw44I       1047  GTGCAC               G^TGCAC
Aor51HI       550  AGCGCT               AGC^GCT
ApaLI        1047  GTGCAC               G^TGCAC
ApoI          943  AAATTC               [GA]^AATT[CT]
BalI          791  TGGCCA               TGG^CCA
BciVI           6  GGATAC               GTATCC(6/5)
BsaI          253  GGTCTC               GGTCTC(1/5)
BsaWI        1287  ACCGGT               [AT]^CCGG[AT]
BsmBI        1129  GAGACG               CGTCTC(1/5)
BspMI          94  ACCTGC               ACCTGC(4/8)
BssHII       1562  GCGCGC               G^CGCGC
BstAPI        821  GCAGCAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Eco47III      550  AGCGCT               AGC^GCT
EcoNI        1191  CCTGCAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
MaeII         486  ACGT                 A^CGT
MluNI         791  TGGCCA               TGG^CCA
MscI          791  TGGCCA               TGG^CCA
MslI         1380  CACTTCGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI         1927  CATATG               CA^TATG
NspI          466  ACATGC               [GA]CATG^[CT]
PinAI        1287  ACCGGT               A^CCGGT
Psp1406I      485  AACGTT               AA^CGTT
SacI         1752  GAGCTC               GAGCT^C
SapI          295  GAAGAGC              GCTCTTC(1/4)
SbfI         1191  CCTGCAGG             CCTGCA^GG
SexAI        1916  ACCTGGT              A^CC[AT]GGT
SpeI          378  ACTAGT               A^CTAGT
Sse8387I     1191  CCTGCAGG             CCTGCA^GG
Tsp45I       1284  GTCAC                ^GT[GC]AC
Tsp509I       944  AATT                 ^AATT
XbaI          427  TCTAGA               T^CTAGA
XcmI         1031  CCAAGGAGGATCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AccI          183  GTCTAC               GT^[AC][GT]AC
              220  GTAGAC              
AlwI          431  GATCC                GGATC(4/5)
             1038  GGATC               
AlwNI         822  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1552  CAGAAGCTG           
AspHI        1047  GTGCAC               G[AT]GC[AT]^C
             1752  GAGCTC              
BbvCI         227  CCTCAGC              CCTCAGC(-5/-2)
              846  CCTCAGC             
BcgI         1076  GCAGCTACTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1148  GCAGCGCCTTCG        
BglI         1177  GCCCGCGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1335  GCCCAGCCGGC         
BpmI         1645  CTCCAG               CTGGAG(16/14)
             2043  CTCCAG              
BsiHKAI      1047  GTGCAC               G[AT]GC[AT]^C
             1752  GAGCTC              
BsmI          111  GAATGC               GAATGC(1/-1)
              475  GAATGC              
BsrDI        1889  GCAATG               GCAATG(2/0)
             2012  GCAATG              
BstXI         794  CCAGGGAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1239  CCAGGACGCTGG        
BstYI         430  AGATCC               [GA]^GATC[CT]
             1038  GGATCT              
DpnII         431  GATC                 ^GATC
             1039  GATC                
MboI          431  GATC                 ^GATC
             1039  GATC                
MflI          430  AGATCC               [GA]^GATC[CT]
             1038  GGATCT              
NdeII         431  GATC                 ^GATC
             1039  GATC                
NotI         1276  GCGGCCGC             GC^GGCCGC
             1786  GCGGCCGC            
PaeR7I       1345  CTCGAG               C^TCGAG
             1567  CTCGAG              
PflMI         783  CCACAACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1551  CCAGAAGCTGG         
PpuMI         669  GGGTCCC              [GA]G^G[AT]CC[CT]
             1678  GGGTCCC             
Sau3AI        431  GATC                 ^GATC
             1039  GATC                
SmaI          666  CCCGGG               CCC^GGG
              812  CCCGGG              
TaqI         1346  TCGA                 T^CGA
             1568  TCGA                
TthHB8I      1346  TCGA                 T^CGA
             1568  TCGA                
Van91I        783  CCACAACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1551  CCAGAAGCTGG         
XhoI         1345  CTCGAG               C^TCGAG
             1567  CTCGAG              
XhoII         430  AGATCC               [GA]^GATC[CT]
             1038  GGATCT              
XmaCI         666  CCCGGG               C^CCGGG
              812  CCCGGG              
XmaI          666  CCCGGG               C^CCGGG
              812  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   93 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
ApaI        AvaI        AvaII       BanI        BanII       
BbeI        BbvI        BcnI        BfaI        BmyI        
BsaHI       BsaJI       BseRI       BsiEI       BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BsrBI       BsrFI       BsrI        BssKI       BstF5I      
BstNI       BstUI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DraII       DsaI        EaeI        
EagI        EarI        EclXI       Eco52I      EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        KasI        Ksp632I     KspI        MaeI        
MaeIII      MboII       MnlI        MspA1I      MspI        
MvaI        MvnI        MwoI        NaeI        NarI        
NciI        NgoMIV      NlaIII      NlaIV       PleI        
PstI        PvuII       RsaI        SacII       Sau96I      
ScrFI       SfaNI       SfcI        SfoI        SgrAI       
SmlI        StyI        TseI        
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