Restriction map for pF1KB7278
 (Linear) (Six-base) MAPSORT of: KIBB7278.seq  Check: 6355  from: 1  to: 2067



KIBB7278.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:07  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     103    2067 
   Size:        103    1964
 
AccI GT'mk_AC

Cuts at:      0     184     221    2067 
   Size:        184      37    1846

  Fragments arranged by size: 

                1846     184      37
 
AclI AA'CG_TT

Cuts at:      0     486    2067 
   Size:        486    1581
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2007    2067 
   Size:       2007      60
 
AfeI AGC'GCT

Cuts at:      0     552    2067 
   Size:        552    1515
 
AgeI A'CCGG_T

Cuts at:      0    1287    2067 
   Size:       1287     780
 
AleI CACnn'nnGTG

Cuts at:      0    1384    2067 
   Size:       1384     683
 
AlwNI CAG_nnn'CTG

Cuts at:      0     827    1557    2067 
   Size:        827     730     510

  Fragments arranged by size: 

                 827     730     510
 
ApaI G_GGCC'C

Cuts at:      0    1330    1600    1799    2067 
   Size:       1330     270     199     268

  Fragments arranged by size: 

                1330     270     268     199
 
ApaLI G'TGCA_C

Cuts at:      0    1047    2067 
   Size:       1047    1020
 
ApoI r'AATT_y

Cuts at:      0     943    2067 
   Size:        943    1124
 
AvaI C'yCGr_G

Cuts at:      0     666     812    1071    1126    1345    1567    2067 
   Size:        666     146     259      55     219     222     500

  Fragments arranged by size: 

                 666     500     259     222     219     146      55
 
BanI G'GyrC_C

Cuts at:      0     592     654    1294    1386    1435    1471    2067 
   Size:        592      62     640      92      49      36     596

  Fragments arranged by size: 

                 640     596     592      92      62      49      36
 
BanII G_rGCy'C

Cuts at:      0    1330    1600    1720    1756    1799    1967    2067 
   Size:       1330     270     120      36      43     168     100

  Fragments arranged by size: 

                1330     270     168     120     100      43      36
 
BbeI G_GCGC'C

Cuts at:      0    1298    1439    1475    2067 
   Size:       1298     141      36     592

  Fragments arranged by size: 

                1298     592     141      36
 
BbvCI CC'TCA_GC

Cuts at:      0     228     847    2067 
   Size:        228     619    1220

  Fragments arranged by size: 

                1220     619     228
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1065    1099    1137    1171    2067 
   Size:       1065      34      38      34     896

  Fragments arranged by size: 

                1065     896      38      34      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1183    1341    2067 
   Size:       1183     158     726

  Fragments arranged by size: 

                1183     726     158
 
Bme1580I G_kGCm'C

Cuts at:      0     595    1051    1330    1600    1799    2005    2067 
   Size:        595     456     279     270     199     206      62

  Fragments arranged by size: 

                 595     456     279     270     206     199      62
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1630    2028    2067 
   Size:       1630     398      39

  Fragments arranged by size: 

                1630     398      39
 
Bpu10I CC'TnA_GC

Cuts at:      0     136     228     847    1709    2067 
   Size:        136      92     619     862     358

  Fragments arranged by size: 

                 862     619     358     136      92
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     163    1981    2067 
   Size:        163    1818      86

  Fragments arranged by size: 

                1818     163      86
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     259    2067 
   Size:        259    1808
 
BsaHI Gr'CG_yC

Cuts at:      0    1295    1436    1472    2067 
   Size:       1295     141      36     595

  Fragments arranged by size: 

                1295     595     141      36
 
BsaWI w'CCGG_w

Cuts at:      0    1287    2067 
   Size:       1287     780
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2026    2056    2067 
   Size:       2026      30      11

  Fragments arranged by size: 

                2026      30      11
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     385     967    1213    1254    2002    2067 
   Size:        385     582     246      41     748      65

  Fragments arranged by size: 

                 748     582     385     246      65      41
 
BseYI C'CCAG_C

Cuts at:      0     834    1336    1395    1545    1557    2067 
   Size:        834     502      59     150      12     510

  Fragments arranged by size: 

                 834     510     502     150      59      12
 
BsiEI CG_ry'CG

Cuts at:      0    1104    1280    1790    2067 
   Size:       1104     176     510     277

  Fragments arranged by size: 

                1104     510     277     176
 
BsiHKAI G_wGCw'C

Cuts at:      0    1051    1756    2067 
   Size:       1051     705     311

  Fragments arranged by size: 

                1051     705     311
 
BsmI GAATG_Cn'

Cuts at:      0     117     481    2067 
   Size:        117     364    1586

  Fragments arranged by size: 

                1586     364     117
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1123    2067 
   Size:       1123     944
 
Bsp1286I G_dGCh'C

Cuts at:      0     595    1051    1330    1600    1720    1756    1799    1967 
   Size:        595     456     279     270     120      36      43     168

Cuts at:   1967    2005    2067 
   Size:         38      62

  Fragments arranged by size: 

                 595     456     279     270     168     120      62      43
                  38      36
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     103    2067 
   Size:        103    1964
 
BsrBI CCG'CTC

Cuts at:      0     344    1014    1691    2067 
   Size:        344     670     677     376

  Fragments arranged by size: 

                 677     670     376     344
 
BsrDI GCAATG_nn'

Cuts at:      0    1896    2019    2067 
   Size:       1896     123      48

  Fragments arranged by size: 

                1896     123      48
 
BsrFI r'CCGG_y

Cuts at:      0     688     748    1287    1340    1979    2067 
   Size:        688      60     539      53     639      88

  Fragments arranged by size: 

                 688     639     539      88      60      53
 
BssHII G'CGCG_C

Cuts at:      0    1562    2067 
   Size:       1562     505
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     827    2067 
   Size:        827    1240
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     801    1246    2067 
   Size:        801     445     821

  Fragments arranged by size: 

                 821     801     445
 
BstYI r'GATC_y

Cuts at:      0     430    1038    2067 
   Size:        430     608    1029

  Fragments arranged by size: 

                1029     608     430
 
BtgI C'CryG_G

Cuts at:      0     603     917    1274    1315    1622    1655    1772    1790 
   Size:        603     314     357      41     307      33     117      18

Cuts at:   1790    2067 
   Size:        277

  Fragments arranged by size: 

                 603     357     314     307     277     117      41      33
                  18
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     897    1972    2067 
   Size:        897    1075      95

  Fragments arranged by size: 

                1075     897      95
 
EaeI y'GGCC_r

Cuts at:      0     281     791     879    1101    1184    1277    1787    2067 
   Size:        281     510      88     222      83      93     510     280

  Fragments arranged by size: 

                 510     510     281     280     222      93      88      83
            
 
EagI C'GGCC_G

Cuts at:      0    1101    1277    1787    2067 
   Size:       1101     176     510     280

  Fragments arranged by size: 

                1101     510     280     176
 
EarI CTCTTCn'nnn_

Cuts at:      0     290    1088    1114    2067 
   Size:        290     798      26     953

  Fragments arranged by size: 

                 953     798     290      26
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     907     976    1442    2067 
   Size:        907      69     466     625

  Fragments arranged by size: 

                 907     625     466      69
 
EcoICRI GAG'CTC

Cuts at:      0    1754    2067 
   Size:       1754     313
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1630    2007    2028    2067 
   Size:       1630     377      21      39

  Fragments arranged by size: 

                1630     377      39      21
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1195    2067 
   Size:       1195     872
 
EcoO109I rG'GnC_Cy

Cuts at:      0     670    1326    1597    1679    1795    1796    2067 
   Size:        670     656     271      82     116       1     271

  Fragments arranged by size: 

                 670     656     271     271     116      82       1
 
HaeII r_GCGC'y

Cuts at:      0     554     980    1154    1298    1376    1439    1475    1499 
   Size:        554     426     174     144      78      63      36      24

Cuts at:   1499    1825    1980    2034    2067 
   Size:        326     155      54      33

  Fragments arranged by size: 

                 554     426     326     174     155     144      78      63
                  54      36      33      24
 
KasI G'GCGC_C

Cuts at:      0    1294    1435    1471    2067 
   Size:       1294     141      36     596

  Fragments arranged by size: 

                1294     596     141      36
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      78    1460    1937    2067 
   Size:         78    1382     477     130

  Fragments arranged by size: 

                1382     477     130      78
 
MscI TGG'CCA

Cuts at:      0     793    2067 
   Size:        793    1274
 
MslI CAynn'nnrTG

Cuts at:      0    1384    2067 
   Size:       1384     683
 
MspA1I CmG'CkG

Cuts at:      0     333     530     742     827     854     866    1276    1317 
   Size:        333     197     212      85      27      12     410      41

Cuts at:   1317    1332    1395    1418    1530    1539    1624    1657    1759 
   Size:         15      63      23     112       9      85      33     102

Cuts at:   1759    1765    1774    1792    1806    1812    2067 
   Size:          6       9      18      14       6     255

  Fragments arranged by size: 

                 410     333     255     212     197     112     102      85
                  85      63      41      33      27      23      18      15
                  14      12       9       9       6       6
 
NaeI GCC'GGC

Cuts at:      0     690     750    1342    1981    2067 
   Size:        690      60     592     639      86

  Fragments arranged by size: 

                 690     639     592      86      60
 
NarI GG'CG_CC

Cuts at:      0    1295    1436    1472    2067 
   Size:       1295     141      36     595

  Fragments arranged by size: 

                1295     595     141      36
 
NdeI CA'TA_TG

Cuts at:      0    1928    2067 
   Size:       1928     139
 
NgoMIV G'CCGG_C

Cuts at:      0     688     748    1340    1979    2067 
   Size:        688      60     592     639      88

  Fragments arranged by size: 

                 688     639     592      88      60
 
NotI GC'GGCC_GC

Cuts at:      0    1277    1787    2067 
   Size:       1277     510     280

  Fragments arranged by size: 

                1277     510     280
 
NspI r_CATG'y

Cuts at:      0     470    2067 
   Size:        470    1597
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     789    1557    2067 
   Size:        789     768     510

  Fragments arranged by size: 

                 789     768     510
 
PfoI T'CCnGG_A

Cuts at:      0     947    2067 
   Size:        947    1120
 
PpuMI rG'GwC_Cy

Cuts at:      0     670    1679    2067 
   Size:        670    1009     388

  Fragments arranged by size: 

                1009     670     388
 
PspOMI G'GGCC_C

Cuts at:      0    1326    1596    1795    2067 
   Size:       1326     270     199     272

  Fragments arranged by size: 

                1326     272     270     199
 
PspXI vC'TCGA_Gb

Cuts at:      0    1345    1567    2067 
   Size:       1345     222     500

  Fragments arranged by size: 

                1345     500     222
 
PstI C_TGCA'G

Cuts at:      0    1196    1770    1811    2067 
   Size:       1196     574      41     256

  Fragments arranged by size: 

                1196     574     256      41
 
PvuII CAG'CTG

Cuts at:      0     333     827    1395    1530    1806    1812    2067 
   Size:        333     494     568     135     276       6     255

  Fragments arranged by size: 

                 568     494     333     276     255     135       6
 
SacI G_AGCT'C

Cuts at:      0    1756    2067 
   Size:       1756     311
 
SacII CC_GC'GG

Cuts at:      0    1277    1318    1625    1658    1775    1793    2067 
   Size:       1277      41     307      33     117      18     274

  Fragments arranged by size: 

                1277     307     274     117      41      33      18
 
SanDI GG'GwC_CC

Cuts at:      0     670    1679    2067 
   Size:        670    1009     388

  Fragments arranged by size: 

                1009     670     388
 
SapI GCTCTTCn'nnn_

Cuts at:      0     290    2067 
   Size:        290    1777
 
SbfI CC_TGCA'GG

Cuts at:      0    1196    2067 
   Size:       1196     871
 
SexAI A'CCwGG_T

Cuts at:      0    1916    2067 
   Size:       1916     151
 
SfcI C'TryA_G

Cuts at:      0    1192    1766    1807    2067 
   Size:       1192     574      41     260

  Fragments arranged by size: 

                1192     574     260      41
 
SfoI GGC'GCC

Cuts at:      0    1296    1437    1473    2067 
   Size:       1296     141      36     594

  Fragments arranged by size: 

                1296     594     141      36
 
SgrAI Cr'CCGG_yG

Cuts at:      0     748    1287    1979    2067 
   Size:        748     539     692      88

  Fragments arranged by size: 

                 748     692     539      88
 
SmaI CCC'GGG

Cuts at:      0     668     814    2067 
   Size:        668     146    1253

  Fragments arranged by size: 

                1253     668     146
 
SmlI C'TyrA_G

Cuts at:      0     178    1345    1567    1996    2067 
   Size:        178    1167     222     429      71

  Fragments arranged by size: 

                1167     429     222     178      71
 
SpeI A'CTAG_T

Cuts at:      0     378    2067 
   Size:        378    1689
 
StyI C'CwwG_G

Cuts at:      0      44     596    1031    1967    2067 
   Size:         44     552     435     936     100

  Fragments arranged by size: 

                 936     552     435     100      44
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     694     726    1030    1062    2026    2058    2067 
   Size:        694      32     304      32     964      32       9

  Fragments arranged by size: 

                 964     694     304      32      32      32       9
 
XbaI T'CTAG_A

Cuts at:      0     427    2067 
   Size:        427    1640
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1038    2067 
   Size:       1038    1029
 
XhoI C'TCGA_G

Cuts at:      0    1345    1567    2067 
   Size:       1345     222     500

  Fragments arranged by size: 

                1345     500     222
 
XmaI C'CCGG_G

Cuts at:      0     666     812    2067 
   Size:        666     146    1255

  Fragments arranged by size: 

                1255     666     146
 

 Enzymes that do cut:

     AarI     AccI     AclI     AcuI     AfeI     AgeI     AleI    AlwNI
     ApaI    ApaLI     ApoI     AvaI     BanI    BanII     BbeI    BbvCI
     BcgI     BglI Bme1580I     BpmI   Bpu10I    BpuEI     BsaI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI   BssHII   BstAPI    BstXI
    BstYI     BtgI    BtgZI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    HaeII     KasI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NdeI   NgoMIV     NotI     NspI    PflMI
     PfoI    PpuMI   PspOMI    PspXI     PstI    PvuII     SacI    SacII
    SanDI     SapI     SbfI    SexAI     SfcI     SfoI    SgrAI     SmaI
     SmlI     SpeI     StyI     TstI     XbaI     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I    AflII   AflIII     AhdI     AlfI     AloI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbsI    BciVI     BclI
    BfrBI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI    BsaAI
    BsaBI     BsgI    BsiWI    BspEI    BspHI    BsrGI    BssSI    BstBI
   BstEII  BstZ17I   Bsu36I     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    Hin4I
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     NcoI     NheI
     NruI     NsiI     PacI     PasI     PciI     PmeI     PmlI     PpiI
    PshAI     PsiI     PsrI     PvuI    RsrII     SalI     ScaI     SfiI
     SgfI    SnaBI     SphI     SrfI     SspI     StuI     SwaI    TaqII
     TatI  Tth111I     XmnI     ZraI
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