Restriction map for pF1KB9928
 (Linear) (Six-base) MAPSORT of: KIBB9928.seq  Check: 2983  from: 1  to: 1878



KIBB9928.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:09  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      59     805     979    1384    1765    1878 
   Size:         59     746     174     405     381     113

  Fragments arranged by size: 

                 746     405     381     174     113      59
 
AfeI AGC'GCT

Cuts at:      0     516    1878 
   Size:        516    1362
 
AflIII A'CryG_T

Cuts at:      0    1460    1878 
   Size:       1460     418
 
ApoI r'AATT_y

Cuts at:      0     423     801    1878 
   Size:        423     378    1077

  Fragments arranged by size: 

                1077     423     378
 
AvaI C'yCGr_G

Cuts at:      0    1299    1878 
   Size:       1299     579
 
BanI G'GyrC_C

Cuts at:      0    1466    1878 
   Size:       1466     412
 
BanII G_rGCy'C

Cuts at:      0    1697    1878 
   Size:       1697     181
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     287    1878 
   Size:        287    1591
 
BbvCI CC'TCA_GC

Cuts at:      0    1378    1441    1878 
   Size:       1378      63     437

  Fragments arranged by size: 

                1378     437      63
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     749     783    1878 
   Size:        749      34    1095

  Fragments arranged by size: 

                1095     749      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     349    1878 
   Size:        349    1529
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1070    1878 
   Size:       1070     808
 
BmgBI CAC'GTC

Cuts at:      0    1461    1878 
   Size:       1461     417
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1660    1737    1878 
   Size:       1660      77     141

  Fragments arranged by size: 

                1660     141      77
 
BmtI G_CTAG'C

Cuts at:      0     515    1878 
   Size:        515    1363
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     685     943    1235    1668    1780    1816    1878 
   Size:        685     258     292     433     112      36      62

  Fragments arranged by size: 

                 685     433     292     258     112      62      36
 
Bpu10I CC'TnA_GC

Cuts at:      0     809    1378    1441    1878 
   Size:        809     569      63     437

  Fragments arranged by size: 

                 809     569     437      63
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1333    1878 
   Size:       1333     545
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1150    1234    1772    1878 
   Size:       1150      84     538     106

  Fragments arranged by size: 

                1150     538     106      84
 
BsaAI yAC'GTr

Cuts at:      0     302    1878 
   Size:        302    1576
 
BsaWI w'CCGG_w

Cuts at:      0     321     459    1878 
   Size:        321     138    1419

  Fragments arranged by size: 

                1419     321     138
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     664     694     916     946    1214    1244    1429    1459 
   Size:        664      30     222      30     268      30     185      30

Cuts at:   1459    1878 
   Size:        419

  Fragments arranged by size: 

                 664     419     268     222     185      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     138    1222    1671    1878 
   Size:        138    1084     449     207

  Fragments arranged by size: 

                1084     449     207     138
 
BseYI C'CCAG_C

Cuts at:      0     683    1878 
   Size:        683    1195
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1017    1747    1878 
   Size:       1017     730     131

  Fragments arranged by size: 

                1017     730     131
 
BsiEI CG_ry'CG

Cuts at:      0    1074    1878 
   Size:       1074     804
 
BsiHKAI G_wGCw'C

Cuts at:      0       7    1878 
   Size:          7    1871
 
BsmI GAATG_Cn'

Cuts at:      0      55    1878 
   Size:         55    1823
 
Bsp1286I G_dGCh'C

Cuts at:      0       7    1697    1878 
   Size:          7    1690     181

  Fragments arranged by size: 

                1690     181       7
 
BspEI T'CCGG_A

Cuts at:      0     321    1878 
   Size:        321    1557
 
BsrDI GCAATG_nn'

Cuts at:      0     438    1565    1878 
   Size:        438    1127     313

  Fragments arranged by size: 

                1127     438     313
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     251    1185    1310    1878 
   Size:        251     934     125     568

  Fragments arranged by size: 

                 934     568     251     125
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      61     856    1455    1878 
   Size:         61     795     599     423

  Fragments arranged by size: 

                 795     599     423      61
 
BstYI r'GATC_y

Cuts at:      0    1150    1847    1878 
   Size:       1150     697      31

  Fragments arranged by size: 

                1150     697      31
 
BtgI C'CryG_G

Cuts at:      0      60    1878 
   Size:         60    1818
 
BtsI GCAGTG_nn'

Cuts at:      0     168     450    1878 
   Size:        168     282    1428

  Fragments arranged by size: 

                1428     282     168
 
EaeI y'GGCC_r

Cuts at:      0    1071    1812    1878 
   Size:       1071     741      66

  Fragments arranged by size: 

                1071     741      66
 
EagI C'GGCC_G

Cuts at:      0    1071    1878 
   Size:       1071     807
 
EarI CTCTTCn'nnn_

Cuts at:      0     962    1878 
   Size:        962     916
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1008    1878 
   Size:       1008     870
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      59     685     805     943     979    1235    1384    1668 
   Size:         59     626     120     138      36     256     149     284

Cuts at:   1668    1765    1780    1816    1878 
   Size:         97      15      36      62

  Fragments arranged by size: 

                 626     284     256     149     138     120      97      62
                  59      36      36      15
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1705    1878 
   Size:       1705     173
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1353    1878 
   Size:       1353     525
 
EcoRI G'AATT_C

Cuts at:      0     423    1878 
   Size:        423    1455
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1158    1878 
   Size:       1158     720
 
HaeII r_GCGC'y

Cuts at:      0     159     518    1878 
   Size:        159     359    1360

  Fragments arranged by size: 

                1360     359     159
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     114     146     802     834     888     920    1214    1246 
   Size:        114      32     656      32      54      32     294      32

Cuts at:   1246    1429    1461    1878 
   Size:        183      32     417

  Fragments arranged by size: 

                 656     417     294     183     114      54      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0    1101    1878 
   Size:       1101     777
 
MscI TGG'CCA

Cuts at:      0    1814    1878 
   Size:       1814      64
 
MslI CAynn'nnrTG

Cuts at:      0      59     443    1453    1666    1878 
   Size:         59     384    1010     213     212

  Fragments arranged by size: 

                1010     384     213     212      59
 
MspA1I CmG'CkG

Cuts at:      0     852    1878 
   Size:        852    1026
 
NcoI C'CATG_G

Cuts at:      0      60    1878 
   Size:         60    1818
 
NheI G'CTAG_C

Cuts at:      0     511    1878 
   Size:        511    1367
 
NspI r_CATG'y

Cuts at:      0     494    1878 
   Size:        494    1384
 
PfoI T'CCnGG_A

Cuts at:      0     104     503    1037    1878 
   Size:        104     399     534     841

  Fragments arranged by size: 

                 841     534     399     104
 
PmlI CAC'GTG

Cuts at:      0     302    1878 
   Size:        302    1576
 
PpuMI rG'GwC_Cy

Cuts at:      0    1353    1878 
   Size:       1353     525
 
PsiI TTA'TAA

Cuts at:      0     545    1878 
   Size:        545    1333
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     655     687    1878 
   Size:        655      32    1191

  Fragments arranged by size: 

                1191     655      32
 
PstI C_TGCA'G

Cuts at:      0    1190    1878 
   Size:       1190     688
 
PvuII CAG'CTG

Cuts at:      0     852    1878 
   Size:        852    1026
 
SapI GCTCTTCn'nnn_

Cuts at:      0     962    1878 
   Size:        962     916
 
ScaI AGT'ACT

Cuts at:      0    1318    1878 
   Size:       1318     560
 
SfcI C'TryA_G

Cuts at:      0    1186    1878 
   Size:       1186     692
 
SmlI C'TyrA_G

Cuts at:      0    1348    1878 
   Size:       1348     530
 
SphI G_CATG'C

Cuts at:      0     494    1878 
   Size:        494    1384
 
StuI AGG'CCT

Cuts at:      0    1255    1819    1861    1878 
   Size:       1255     564      42      17

  Fragments arranged by size: 

                1255     564      42      17
 
StyI C'CwwG_G

Cuts at:      0      60    1265    1286    1878 
   Size:         60    1205      21     592

  Fragments arranged by size: 

                1205     592      60      21
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1852    1878 
   Size:       1852      26
 
TatI w'GTAC_w

Cuts at:      0    1316    1878 
   Size:       1316     562
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     916     948    1450    1482    1777    1809    1878 
   Size:        916      32     502      32     295      32      69

  Fragments arranged by size: 

                 916     502     295      69      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1226    1878 
   Size:       1226     652
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     181     918    1878 
   Size:        181     737     960

  Fragments arranged by size: 

                 960     737     181
 

 Enzymes that do cut:

     AcuI     AfeI   AflIII     ApoI     AvaI     BanI    BanII     BbsI
    BbvCI     BcgI    BciVI     BglI    BmgBI     BmrI     BmtI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BsrDI   BstAPI
    BstXI    BstYI     BtgI     BtsI     EaeI     EagI     EarI     EciI
  Eco57MI    EcoNI EcoO109I    EcoRI     FalI    HaeII    Hin4I   HincII
     MscI     MslI   MspA1I     NcoI     NheI     NspI     PfoI     PmlI
    PpuMI     PsiI     PsrI     PstI    PvuII     SapI     ScaI     SfcI
     SmlI     SphI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbeI     BclI    BfrBI    BglII
     BlpI Bme1580I     BplI    BsaBI    BsaHI    BsiWI    BsmBI    BspHI
    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
  EcoICRI    EcoRV     FseI     FspI    FspAI  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     NaeI     NarI     NdeI   NgoMIV
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PmeI
     PpiI    PshAI   PspOMI    PspXI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SrfI     SspI     SwaI     XbaI     XhoI
     XmaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com