Restriction map for
pF1KB7690
(Circular) (Six-base) MAPSORT of: pF1KB7690.seq Check: 1125 from: 1 to: 4346
pF1KB7690 4346 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AccI GT'mk_AC
Cuts at: 579 1192 1229 1384 579
Size: 613 37 155 3541
Fragments arranged by size:
3541 613 155 37
Acc65I G'GTAC_C
Cuts at: 1362 1362
Size: 4346
AclI AA'CG_TT
Cuts at: 4186 4186
Size: 4346
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 656 2171 2603 3350 3618 656
Size: 1515 432 747 268 1384
Fragments arranged by size:
1515 1384 747 432 268
AflIII A'CryG_T
Cuts at: 1698 2802 3779 1698
Size: 1104 977 2265
Fragments arranged by size:
2265 1104 977
AgeI A'CCGG_T
Cuts at: 2734 2734
Size: 4346
AhdI GACnn_n'nnGTC
Cuts at: 753 753
Size: 4346
AlwNI CAG_nnn'CTG
Cuts at: 151 559 1651 3218 151
Size: 408 1092 1567 1279
Fragments arranged by size:
1567 1279 1092 408
ApaLI G'TGCA_C
Cuts at: 3116 3116
Size: 4346
ApoI r'AATT_y
Cuts at: 722 880 1350 3699 3955 722
Size: 158 470 2349 256 1113
Fragments arranged by size:
2349 1113 470 256 158
AseI AT'TA_AT
Cuts at: 20 20
Size: 4346
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4346
AvaI C'yCGr_G
Cuts at: 240 1366 240
Size: 1126 3220
BamHI G'GATC_C
Cuts at: 1371 1371
Size: 4346
BanI G'GyrC_C
Cuts at: 741 1362 1776 2024 2059 741
Size: 621 414 248 35 3028
Fragments arranged by size:
3028 621 414 248 35
BanII G_rGCy'C
Cuts at: 1360 2390 1360
Size: 1030 3316
BbeI G_GCGC'C
Cuts at: 1780 2028 1780
Size: 248 4098
BciVI GTATCCnnnnn_n'
Cuts at: 181 2244 3005 4346 181
Size: 2063 761 1341 181
Fragments arranged by size:
2063 1341 761 181
BclI T'GATC_A
Cuts at: 1866 1866
Size: 4346
BglII A'GATC_T
Cuts at: 423 1861 3898 423
Size: 1438 2037 871
Fragments arranged by size:
2037 1438 871
BlpI GC'TnA_GC
Cuts at: 1040 1458 1040
Size: 418 3928
Bme1580I G_kGCm'C
Cuts at: 1971 2064 3120 1971
Size: 93 1056 3197
Fragments arranged by size:
3197 1056 93
BmgBI CAC'GTC
Cuts at: 1190 1190
Size: 4346
BmrI ACTGGGnnnn_n'
Cuts at: 1558 1639 1974 4118 1558
Size: 81 335 2144 1786
Fragments arranged by size:
2144 1786 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 608 2713 608
Size: 2105 2241
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1522 2401 2893 3191 1522
Size: 879 492 298 2677
Fragments arranged by size:
2677 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4050 4050
Size: 4346
BsaAI yAC'GTr
Cuts at: 1699 1699
Size: 4346
BsaBI GATnn'nnATC
Cuts at: 6 1860 3897 6
Size: 1854 2037 455
Fragments arranged by size:
2037 1854 455
BsaHI Gr'CG_yC
Cuts at: 1777 2025 2727 4223 1777
Size: 248 702 1496 1900
Fragments arranged by size:
1900 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1535 2056 2734 3008 3155 3566 1535
Size: 521 678 274 147 411 2315
Fragments arranged by size:
2315 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 132 162 1179 1209 132
Size: 30 1017 30 3269
Fragments arranged by size:
3269 1017 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1111 1150 1312 1111
Size: 39 162 4145
Fragments arranged by size:
4145 162 39
BseYI C'CCAG_C
Cuts at: 144 166 228 1771 2529 3106 3602 144
Size: 22 62 1543 758 577 496 888
Fragments arranged by size:
1543 888 758 577 496 62 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 572 572
Size: 4346
BsiEI CG_ry'CG
Cuts at: 104 1888 1934 3142 104
Size: 1784 46 1208 1308
Fragments arranged by size:
1784 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 247 1360 2138 2328 3120 247
Size: 1113 778 190 792 1473
Fragments arranged by size:
1473 1113 792 778 190
BsmI GAATG_Cn'
Cuts at: 623 782 623
Size: 159 4187
Bsp1286I G_dGCh'C
Cuts at: 247 1360 1971 2064 2138 2328 2390 3120 247
Size: 1113 611 93 74 190 62 730 1473
Fragments arranged by size:
1473 1113 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3566 3566
Size: 4346
BspHI T'CATG_A
Cuts at: 3522 3522
Size: 4346
BspMI ACCTGCnnnn'nnnn_
Cuts at: 771 786 1396 1610 1912 2293 771
Size: 15 610 214 302 381 2824
Fragments arranged by size:
2824 610 381 302 214 15
BsrBI CCG'CTC
Cuts at: 50 2638 2692 4176 4341 50
Size: 2588 54 1484 165 55
Fragments arranged by size:
2588 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2258 2258
Size: 4346
BsrFI r'CCGG_y
Cuts at: 2344 2525 2734 2344
Size: 181 209 3956
Fragments arranged by size:
3956 209 181
BssSI C'ACGA_G
Cuts at: 2617 2975 2617
Size: 358 3988
BstAPI GCAn_nnn'nTGC
Cuts at: 559 1723 559
Size: 1164 3182
BstBI TT'CG_AA
Cuts at: 2707 2707
Size: 4346
BstXI CCAn_nnnn'nTGG
Cuts at: 1174 1174
Size: 4346
BstYI r'GATC_y
Cuts at: 1 423 639 1311 1371 1838 1861 2196 2442
Size: 422 216 672 60 467 23 335 246
Cuts at: 2442 3454 3540 3552 3898 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 672 467 449 422 346 335 246
216 86 60 23 12
Bsu36I CC'TnA_GG
Cuts at: 1067 1199 1067
Size: 132 4214
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2296 2477 2640 2296
Size: 181 163 4002
Fragments arranged by size:
4002 181 163
BtsI GCAGTG_nn'
Cuts at: 1589 1589
Size: 4346
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1194 1229 1194
Size: 35 4311
DraI TTT'AAA
Cuts at: 455 1345 455
Size: 890 3456
DrdI GACnn_nn'nnGTC
Cuts at: 2052 2910 2052
Size: 858 3488
EaeI y'GGCC_r
Cuts at: 1931 2105 2496 2523 3868 1931
Size: 174 391 27 1345 2409
Fragments arranged by size:
2409 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1931 1931
Size: 4346
EarI CTCTTCn'nnn_
Cuts at: 868 2369 2579 868
Size: 1501 210 2635
Fragments arranged by size:
2635 1501 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2554 2864 3010 4156 2554
Size: 310 146 1146 2744
Fragments arranged by size:
2744 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1358 1358
Size: 4346
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 656 2171 2603 3350 3618 656
Size: 1515 432 747 268 1384
Fragments arranged by size:
1515 1384 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 327 327
Size: 4346
EcoO109I rG'GnC_Cy
Cuts at: 783 1485 783
Size: 702 3644
EcoRI G'AATT_C
Cuts at: 1350 1350
Size: 4346
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 140 856 2365 140
Size: 716 1509 2121
Fragments arranged by size:
2121 1509 716
FspI TGC'GCA
Cuts at: 2127 2127
Size: 4346
HaeII r_GCGC'y
Cuts at: 1780 2028 3050 4029 1780
Size: 248 1022 979 2097
Fragments arranged by size:
2097 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 739 771 2188 2218 2220 2250 739
Size: 32 1417 30 2 30 2835
Fragments arranged by size:
2835 1417 32 30 30 2
HincII GTy'rAC
Cuts at: 580 1230 1385 580
Size: 650 155 3541
Fragments arranged by size:
3541 650 155
KasI G'GCGC_C
Cuts at: 1776 2024 1776
Size: 248 4098
KpnI G_GTAC'C
Cuts at: 1366 1366
Size: 4346
MluI A'CGCG_T
Cuts at: 3779 3779
Size: 4346
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 392 3017 3201 392
Size: 2625 184 1537
Fragments arranged by size:
2625 1537 184
MscI TGG'CCA
Cuts at: 2107 2107
Size: 4346
MslI CAynn'nnrTG
Cuts at: 1123 2335 2462 1123
Size: 1212 127 3007
Fragments arranged by size:
3007 1212 127
MspA1I CmG'CkG
Cuts at: 315 967 1457 1771 2131 3144 3389 315
Size: 652 490 314 360 1013 245 1272
Fragments arranged by size:
1272 1013 652 490 360 314 245
NaeI GCC'GGC
Cuts at: 2527 2527
Size: 4346
NarI GG'CG_CC
Cuts at: 1777 2025 1777
Size: 248 4098
NgoMIV G'CCGG_C
Cuts at: 2525 2525
Size: 4346
NspI r_CATG'y
Cuts at: 417 1399 1899 2806 417
Size: 982 500 907 1957
Fragments arranged by size:
1957 982 907 500
PciI A'CATG_T
Cuts at: 2802 2802
Size: 4346
PfoI T'CCnGG_A
Cuts at: 884 884
Size: 4346
PmeI GTTT'AAAC
Cuts at: 1345 1345
Size: 4346
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3485 3517 3485
Size: 32 4314
PpuMI rG'GwC_Cy
Cuts at: 783 783
Size: 4346
PstI C_TGCA'G
Cuts at: 1393 2078 1393
Size: 685 3661
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4346
PvuII CAG'CTG
Cuts at: 967 1771 2131 967
Size: 804 360 3182
Fragments arranged by size:
3182 804 360
RsrII CG'GwC_CG
Cuts at: 2541 2541
Size: 4346
SacI G_AGCT'C
Cuts at: 1360 1360
Size: 4346
SalI G'TCGA_C
Cuts at: 578 1228 1383 578
Size: 650 155 3541
Fragments arranged by size:
3541 650 155
SapI GCTCTTCn'nnn_
Cuts at: 2369 2579 2369
Size: 210 4136
SbfI CC_TGCA'GG
Cuts at: 1393 1393
Size: 4346
ScaI AGT'ACT
Cuts at: 3863 3863
Size: 4346
SfcI C'TryA_G
Cuts at: 33 222 1389 2074 3067 3258 33
Size: 189 1167 685 993 191 1121
Fragments arranged by size:
1167 1121 993 685 191 189
SfoI GGC'GCC
Cuts at: 1778 2026 1778
Size: 248 4098
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4346
SmaI CCC'GGG
Cuts at: 1368 1368
Size: 4346
SmlI C'TyrA_G
Cuts at: 1501 2416 2908 3170 1501
Size: 915 492 262 2677
Fragments arranged by size:
2677 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4346
SphI G_CATG'C
Cuts at: 1399 1899 1399
Size: 500 3846
SspI AAT'ATT
Cuts at: 451 451
Size: 4346
StuI AGG'CCT
Cuts at: 502 502
Size: 4346
StyI C'CwwG_G
Cuts at: 1480 1834 1480
Size: 354 3992
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 743 1929 2062 2521 2730 743
Size: 1186 133 459 209 2359
Fragments arranged by size:
2359 1186 459 209 133
TatI w'GTAC_w
Cuts at: 482 3861 482
Size: 3379 967
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 132 164 1179 1211 3863 3895 132
Size: 32 1015 32 2652 32 583
Fragments arranged by size:
2652 1015 583 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2143 2143
Size: 4346
XbaI T'CTAG_A
Cuts at: 1377 1377
Size: 4346
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1841 1841
Size: 4346
XmaI C'CCGG_G
Cuts at: 1366 1366
Size: 4346
XmnI GAAnn'nnTTC
Cuts at: 842 842
Size: 4346
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PfoI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmtI BpmI Bpu10I BsiWI BsmBI BsrGI BssHII BstEII
BstZ17I BtgI ClaI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SwaI XhoI ZraI