Restriction map for pF1KB6465
##################################
# NO CUT ENZYMES (  180 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AflII       
AflIII      AgeI        Alw44I      AlwNI       Aor51HI     
ApaI        ApaLI       ApoI        AscI        Asp700I     
Asp718I     AspHI       AspI        AvaI        AvaII       
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BfrI        BglI        BlnI        BlpI        
BmyI        BpmI        Bpu1102I    BsaAI       BsaHI       
BsaI        BsaWI       BseAI       BsiEI       BsiHKAI     
BsiWI       BsiYI       BslI        BsmBI       BsmFI       
BsmI        BsoBI       Bsp1286I    Bsp1407I    BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstNI       BstPI       BstUI       
BstXI       BstZ17I     CelII       CfoI        Cfr10I      
Cfr13I      CpoI        DraII       DraIII      DrdI        
EaeI        EagI        EclXI       Eco47III    Eco52I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRII      
EcoRV       EcoT22I     FauI        FseI        FspI        
HaeII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HpaI        KasI        KpnI        
KspI        MaeII       MfeI        MluI        MluNI       
MroI        MscI        MslI        MspA1I      MunI        
MvaI        MvnI        NaeI        NarI        NdeI        
NgoMIV      NheI        NlaIV       NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        Sau96I      SbfI        
ScaI        SexAI       SfaNI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        Tth111I     Van91I      
XbaI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AhdI          499  GACATAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI          604  CCTGAGG              CC^T[ACGT]AGG
AspEI         499  GACATAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BclI          213  TGATCA               T^GATCA
BcnI           87  CCCGG                CC^[GC]GG
BfaI          830  CTAG                 C^TAG
BglII         151  AGATCT               A^GATCT
BsaBI         214  GATCATTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI         655  GAGGAG               GAGGAG(10/8)
BspDI         729  ATCGAT               AT^CGAT
BssKI          87  CCCGG                ^CC[ACGT]GG
BstAPI        808  GCATACTATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I        604  CCTGAGG              CC^T[ACGT]AGG
Cac8I         397  GCCAGC               GC[ACGT]^[ACGT]GC
ClaI          729  ATCGAT               AT^CGAT
DraI          429  TTTAAA               TTT^AAA
DsaI          631  CCATGG               C^C[GA][CT]GG
Eam1105I      499  GACATAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          886  CTCTTC               CTCTTC(1/4)
Eco81I        604  CCTGAGG              CC^T[ACGT]AGG
FbaI          213  TGATCA               T^GATCA
HapII          88  CCGG                 C^CGG
HindIII       109  AAGCTT               A^AGCTT
HpaII          88  CCGG                 C^CGG
HphI          355  GGTGA                GGTGA(8/7)
Ksp632I       886  CTCTTC               CTCTTC(1/4)
MaeI          830  CTAG                 C^TAG
MamI          214  GATCATTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI           88  CCGG                 C^CGG
MwoI          808  GCATACTATGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI           87  CCCGG                CC^[GC]GG
NcoI          631  CCATGG               C^CATGG
PleI          563  GAGTC                GAGTC(4/5)
ScrFI          87  CCCGG                CC^[ACGT]GG
Tsp45I        595  GTGAC                ^GT[GC]AC
TspRI         575  CACTG                CA[GC]TG[ACGT][ACGT]^
XcmI          641  CCAAAGAAGCTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AfaI          122  GTAC                 GT^AC
              138  GTAC                
AlwI          406  GATCC                GGATC(4/5)
                8  GGATC               
BbsI          907  GAAGAC               GAAGAC(2/6)
              507  GTCTTC              
BpuAI         907  GAAGAC               GAAGAC(2/6)
              507  GTCTTC              
BsaJI         178  CCTTGG               C^C[ACGT][ACGT]GG
              631  CCATGG              
BsgI          469  CTGCAC               GTGCAG(16/14)
              932  CTGCAC              
BstYI         151  AGATCT               [GA]^GATC[CT]
              405  AGATCC              
DdeI          257  CTAAG                C^T[ACGT]AG
              605  CTGAG               
EcoT14I       178  CCTTGG               C^C[AT][AT]GG
              631  CCATGG              
MaeIII        595  GTGAC                ^GT[ACGT]AC
              614  GTTAC               
MflI          151  AGATCT               [GA]^GATC[CT]
              405  AGATCC              
NlaIII        493  CATG                 CATG^
              632  CATG                
RsaI          122  GTAC                 GT^AC
              138  GTAC                
StyI          178  CCTTGG               C^C[AT][AT]GG
              631  CCATGG              
TaqI          222  TCGA                 T^CGA
              730  TCGA                
TfiI          732  GATTC                G^A[AT]TC
              862  GAATC               
TthHB8I       222  TCGA                 T^CGA
              730  TCGA                
XhoII         151  AGATCT               [GA]^GATC[CT]
              405  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   23 enzymes) #
#########################################

AluI        AseI        AsnI        BbvI        BsmAI       
BsrI        BstF5I      DpnII       Fnu4HI      FokI        
HaeIII      HinfI       ItaI        MboI        MboII       
MnlI        MseI        NdeII       PshBI       Sau3AI      
Tru9I       TseI        Tsp509I     
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