Restriction map for pF1KB7526
##################################
# NO CUT ENZYMES (  174 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaLI       AscI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AviII       AvrII       
BalI        BamHI       BbrPI       BbsI        BbvCI       
BbvI        BcgI        BciVI       BclI        BcnI        
BfrI        BglI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaI        BsaWI       BseAI       BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssKI       BssSI       Bst1107I    BstBI       BstEII      
BstNI       BstPI       BstUI       BstZ17I     Bsu36I      
Cac8I       CelII       Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoO65I     EcoRI       EcoRII      EcoT22I     
FauI        FbaI        Fnu4HI      FseI        FspI        
HapII       HgaI        HincII      HindII      HindIII     
HpaI        HpaII       ItaI        KpnI        KspI        
MaeII       MamI        MfeI        MluI        MluNI       
MroI        MscI        MspA1I      MspI        MunI        
MvaI        MvnI        NaeI        NciI        NcoI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
Psp1406I    PvuI        PvuII       RcaI        RsaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        ScrFI       SexAI       SfiI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SphI        Sse8387I    SspBI       StuI        SwaI        
TseI        Tth111I     Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AcyI           18  GGCGCC               G[GA]^CG[CT]C
AluI          369  AGCT                 AG^CT
AlwI           63  GATCC                GGATC(4/5)
ApaI          643  GGGCCC               GGGCC^C
AseI          625  ATTAAT               AT^TAAT
AsnI          625  ATTAAT               AT^TAAT
BanI           18  GGCGCC               G^G[CT][GA]CC
BanII         643  GGGCCC               G[GA]GC[CT]^C
BbeI           18  GGCGCC               GGCGC^C
BmyI          643  GGGCCC               G[AGT]GC[ACT]^C
BsaHI          18  GGCGCC               G[GA]^CG[CT]C
BseRI         254  CTCCTC               GAGGAG(10/8)
BsgI          553  GTGCAG               GTGCAG(16/14)
Bsp1286I      643  GGGCCC               G[AGT]GC[ACT]^C
BstAPI        598  GCAACAACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I        153  GGATG                GGATG(2/0)
BstXI         665  CCACCAACATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI          62  AGATCC               [GA]^GATC[CT]
CfoI           19  GCGC                 GCG^C
DpnII          63  GATC                 ^GATC
EarI          714  GAAGAG               CTCTTC(1/4)
EcoNI         409  CCTAACCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV         280  GATATC               GAT^ATC
FokI          153  GGATG                GGATG(9/13)
HaeII          18  GGCGCC               [GA]GCGC^[CT]
HaeIII        644  GGCC                 GG^CC
HhaI           19  GCGC                 GCG^C
Hin1I          18  GGCGCC               G[GA]^CG[CT]C
HinP1I         19  GCGC                 G^CGC
HphI           51  GGTGA                GGTGA(8/7)
KasI           18  GGCGCC               G^GCGCC
Ksp632I       714  GAAGAG               CTCTTC(1/4)
MboI           63  GATC                 ^GATC
MflI           62  AGATCC               [GA]^GATC[CT]
NarI           18  GGCGCC               GG^CGCC
NdeI          326  CATATG               CA^TATG
NdeII          63  GATC                 ^GATC
PacI          394  TTAATTAA             TTAAT^TAA
PleI            4  GAGTC                GAGTC(4/5)
PshBI         625  ATTAAT               AT^TAAT
PstI          605  CTGCAG               CTGCA^G
Sau3AI         63  GATC                 ^GATC
SfoI           18  GGCGCC               GGC^GCC
SpeI          423  ACTAGT               A^CTAGT
SspI          652  AATATT               AAT^ATT
TaqI          172  TCGA                 T^CGA
TthHB8I       172  TCGA                 T^CGA
XhoII          62  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AcsI          121  GAATTT               [GA]^AATT[CT]
              164  AAATTT              
AlwNI          61  CAGATCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              590  CAGTTCCTG           
ApoI          121  GAATTT               [GA]^AATT[CT]
              164  AAATTT              
AvaII         334  GGACC                G^G[AT]CC
              406  GGACC               
BfaI          338  CTAG                 C^TAG
              424  CTAG                
BsmAI         213  GAGAC                GTCTC(1/5)
              420  GAGAC               
BsmFI         405  GGGAC                GGGAC(10/14)
              502  GTCCC               
DdeI          182  CTAAG                C^T[ACGT]AG
              569  CTCAG               
DraII         333  AGGACCT              [GA]G^G[ACGT]CC[CT]
              405  GGGACCT             
EcoO109I      333  AGGACCT              [GA]G^G[ACGT]CC[CT]
              405  GGGACCT             
MaeI          338  CTAG                 C^TAG
              424  CTAG                
MboII         714  GAAGA                GAAGA(8/7)
              727  GAAGA               
MslI          666  CACCAACATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              724  CATGAAGATG          
MwoI          598  GCAACAACTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              694  GCCAATGAAGC         
PpuMI         333  AGGACCT              [GA]G^G[AT]CC[CT]
              405  GGGACCT             
TfiI           80  GAATC                G^A[AT]TC
              558  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (   20 enzymes) #
#########################################

BsaJI       BsiYI       BslI        BsrI        Cfr13I      
EcoT14I     HinfI       MaeIII      MnlI        MseI        
NlaIII      NlaIV       Sau96I      SfaNI       SfcI        
StyI        Tru9I       Tsp45I      Tsp509I     TspRI       
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