Restriction map for pF1KB7995
##################################
# NO CUT ENZYMES (  131 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
Asp718I     AspEI       AspI        AviII       AvrII       
BamHI       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BfaI        BglI        BlnI        
BlpI        Bpu1102I    BsaBI       BsaI        BseAI       
BseRI       BsiEI       BsiWI       BsmBI       BspDI       
BspEI       BspMI       BsrBI       BsrDI       BsrFI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      ClaI        
CpoI        DraIII      DrdI        DsaI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoO65I     EcoRV       FauI        FbaI        FseI        
FspI        HaeII       HhaI        HinP1I      HincII      
HindII      HpaI        KasI        KpnI        KspI        
MaeI        MamI        MfeI        MluI        MroI        
MslI        MunI        MvnI        NaeI        NarI        
NcoI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
Psp1406I    PvuI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SfiI        
SfoI        SfuI        SgrAI       SpeI        SphI        
Sse8387I    SwaI        Tth111I     Van91I      XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AciI         1302  GCGG                 CCGC(-3/-1)
AcyI          385  GGCGTC               G[GA]^CG[CT]C
AflII         889  CTTAAG               C^TTAAG
AflIII        227  ACATGT               A^C[GA][CT]GT
AlwI          753  GGATC                GGATC(4/5)
AseI          522  ATTAAT               AT^TAAT
AsnI          522  ATTAAT               AT^TAAT
Asp700I       799  GAAAAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI          64  GTGCTC               G[AT]GC[AT]^C
AvaI          512  CCCGGG               C^[CT]CG[GA]G
AvaII         708  GGACC                G^G[AT]CC
BanI          861  GGCACC               G^G[CT][GA]CC
BanII         764  GAGCCC               G[GA]GC[CT]^C
BfrI          889  CTTAAG               C^TTAAG
BsaAI         106  TACGTA               [CT]AC^GT[GA]
BsaHI         385  GGCGTC               G[GA]^CG[CT]C
BsiHKAI        64  GTGCTC               G[AT]GC[AT]^C
BsmFI         707  GGGAC                GGGAC(10/14)
BsoBI         512  CCCGGG               C^[CT]CG[GA]G
Bsp1407I      230  TGTACA               T^GTACA
BspHI         389  TCATGA               T^CATGA
BspLU11I      227  ACATGT               A^CATGT
BsrGI         230  TGTACA               T^GTACA
EcoNI         531  CCTTATTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI          386  GCGTC                GACGC(5/10)
Hin1I         385  GGCGTC               G[GA]^CG[CT]C
MaeIII         34  GTGAC                ^GT[ACGT]AC
MspA1I        654  CAGCTG               C[AC]G^C[GT]G
NdeI          791  CATATG               CA^TATG
PpuMI         707  GGGACCC              [GA]G^G[AT]CC[CT]
PshBI         522  ATTAAT               AT^TAAT
PstI          218  CTGCAG               CTGCA^G
PvuII         654  CAGCTG               CAG^CTG
RcaI          389  TCATGA               T^CATGA
SexAI         568  ACCTGGT              A^CC[AT]GGT
SfcI          218  CTGCAG               C^T[GA][CT]AG
SmaI          512  CCCGGG               CCC^GGG
SmlI          889  CTTAAG               C^T[CT][GA]AG
SnaBI         106  TACGTA               TAC^GTA
SspBI         230  TGTACA               T^GTACA
StuI          539  AGGCCT               AGG^CCT
TaqI          756  TCGA                 T^CGA
TfiI          256  GATTC                G^A[AT]TC
Tsp45I         34  GTGAC                ^GT[GC]AC
TthHB8I       756  TCGA                 T^CGA
XcmI          332  CCAAAAGCAACATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         512  CCCGGG               C^CCGGG
XmaI          512  CCCGGG               C^CCGGG
XmnI          799  GAAAAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

BalI          119  TGGCCA               TGG^CCA
             1135  TGGCCA              
BbsI          660  GAAGAC               GAAGAC(2/6)
              507  GTCTTC              
BcnI          512  CCCGG                CC^[GC]GG
              513  CCGGG               
BglII         404  AGATCT               A^GATCT
             1312  AGATCT              
BmyI           64  GTGCTC               G[AGT]GC[ACT]^C
              764  GAGCCC              
BpmI         1119  CTCCAG               CTGGAG(16/14)
              698  CTGGAG              
BpuAI         660  GAAGAC               GAAGAC(2/6)
              507  GTCTTC              
BsaWI         368  ACCGGA               [AT]^CCGG[AT]
             1253  TCCGGT              
BsgI          281  CTGCAC               GTGCAG(16/14)
               74  GTGCAG              
BsmI           80  GAATGC               GAATGC(1/-1)
              443  GAATGC              
Bsp1286I       64  GTGCTC               G[AGT]GC[ACT]^C
              764  GAGCCC              
BsrI          981  ACTGG                ACTGG(1/-1)
             1130  ACTGG               
BstYI         404  AGATCT               [GA]^GATC[CT]
             1312  AGATCT              
Cac8I         213  GCAAGC               GC[ACGT]^[ACGT]GC
              276  GCAGGC              
Cfr13I         28  GGGCC                G^G[ACGT]CC
              708  GGACC               
DraI          399  TTTAAA               TTT^AAA
             1263  TTTAAA              
DraII          27  AGGGCCT              [GA]G^G[ACGT]CC[CT]
              707  GGGACCC             
EaeI          119  TGGCCA               [CT]^GGCC[GA]
             1135  TGGCCA              
EcoO109I       27  AGGGCCT              [GA]G^G[ACGT]CC[CT]
              707  GGGACCC             
EcoRI         742  GAATTC               G^AATTC
              934  GAATTC              
EcoT14I        91  CCTTGG               C^C[AT][AT]GG
              248  CCTTGG              
HindIII       598  AAGCTT               A^AGCTT
             1208  AAGCTT              
MaeII         107  ACGT                 A^CGT
              505  ACGT                
MflI          404  AGATCT               [GA]^GATC[CT]
             1312  AGATCT              
MluNI         119  TGGCCA               TGG^CCA
             1135  TGGCCA              
MscI          119  TGGCCA               TGG^CCA
             1135  TGGCCA              
NciI          512  CCCGG                CC^[GC]GG
              513  CCGGG               
NspI          227  ACATGT               [GA]CATG^[CT]
              417  ACATGC              
PleI         1346  GACTC                GAGTC(4/5)
             1004  GAGTC               
Sau96I         28  GGGCC                G^G[ACGT]CC
              708  GGACC               
StyI           91  CCTTGG               C^C[AT][AT]GG
              248  CCTTGG              
TspRI          12  CAGTG                CA[GC]TG[ACGT][ACGT]^
               69  CAGTG               
XhoII         404  AGATCT               [GA]^GATC[CT]
             1312  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   45 enzymes) #
#########################################

AcsI        AfaI        AluI        ApoI        BbvI        
BsaJI       BsiYI       BslI        BsmAI       BssKI       
BstF5I      BstNI       DdeI        DpnII       EarI        
EcoRII      EcoT22I     Fnu4HI      FokI        HaeIII      
HapII       HinfI       HpaII       HphI        ItaI        
Ksp632I     MboI        MboII       MnlI        MseI        
MspI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       NsiI        RsaI        Sau3AI      ScrFI       
SfaNI       SspI        Tru9I       TseI        Tsp509I     
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