Restriction map for pF1KB7995
 (Linear) (Six-base) MAPSORT of: KIBB7995.seq  Check: 9572  from: 1  to: 1350



KIBB7995.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:08  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     217    1350 
   Size:        217    1133
 
AflII C'TTAA_G

Cuts at:      0     889    1350 
   Size:        889     461
 
AflIII A'CryG_T

Cuts at:      0     227    1350 
   Size:        227    1123
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     233    1350 
   Size:        233    1117
 
ApoI r'AATT_y

Cuts at:      0     314     692     742     934    1350 
   Size:        314     378      50     192     416

  Fragments arranged by size: 

                 416     378     314     192      50
 
AseI AT'TA_AT

Cuts at:      0     523    1350 
   Size:        523     827
 
AvaI C'yCGr_G

Cuts at:      0     512    1350 
   Size:        512     838
 
BanI G'GyrC_C

Cuts at:      0     861    1350 
   Size:        861     489
 
BanII G_rGCy'C

Cuts at:      0     768    1350 
   Size:        768     582
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     500     667    1350 
   Size:        500     167     683

  Fragments arranged by size: 

                 683     500     167
 
BfrBI ATG'CAT

Cuts at:      0      84     421     790    1350 
   Size:         84     337     369     560

  Fragments arranged by size: 

                 560     369     337      84
 
BglII A'GATC_T

Cuts at:      0     404    1312    1350 
   Size:        404     908      38

  Fragments arranged by size: 

                 908     404      38
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     991    1350 
   Size:        991     359
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1134    1350 
   Size:       1134     216
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     719    1104    1350 
   Size:        719     385     246

  Fragments arranged by size: 

                 719     385     246
 
BsaAI yAC'GTr

Cuts at:      0     108    1350 
   Size:        108    1242
 
BsaHI Gr'CG_yC

Cuts at:      0     386    1350 
   Size:        386     964
 
BsaWI w'CCGG_w

Cuts at:      0     368    1253    1350 
   Size:        368     885      97

  Fragments arranged by size: 

                 885     368      97
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     480     510    1350 
   Size:        480      30     840

  Fragments arranged by size: 

                 840     480      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      95     266    1350 
   Size:         95     171    1084

  Fragments arranged by size: 

                1084     171      95
 
BsiHKAI G_wGCw'C

Cuts at:      0      68    1350 
   Size:         68    1282
 
BsmI GAATG_Cn'

Cuts at:      0      86     449    1350 
   Size:         86     363     901

  Fragments arranged by size: 

                 901     363      86
 
Bsp1286I G_dGCh'C

Cuts at:      0      68     768    1350 
   Size:         68     700     582

  Fragments arranged by size: 

                 700     582      68
 
BspHI T'CATG_A

Cuts at:      0     389    1350 
   Size:        389     961
 
BsrGI T'GTAC_A

Cuts at:      0     230    1350 
   Size:        230    1120
 
BstYI r'GATC_y

Cuts at:      0     404    1312    1350 
   Size:        404     908      38

  Fragments arranged by size: 

                 908     404      38
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1093    1128    1350 
   Size:       1093      35     222

  Fragments arranged by size: 

                1093     222      35
 
DraI TTT'AAA

Cuts at:      0     401    1265    1350 
   Size:        401     864      85

  Fragments arranged by size: 

                 864     401      85
 
EaeI y'GGCC_r

Cuts at:      0     119    1135    1350 
   Size:        119    1016     215

  Fragments arranged by size: 

                1016     215     119
 
EarI CTCTTCn'nnn_

Cuts at:      0     715    1103    1303    1350 
   Size:        715     388     200      47

  Fragments arranged by size: 

                 715     388     200      47
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     217     719    1104    1350 
   Size:        217     502     385     246

  Fragments arranged by size: 

                 502     385     246     217
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     535    1350 
   Size:        535     815
 
EcoO109I rG'GnC_Cy

Cuts at:      0      28     708    1350 
   Size:         28     680     642

  Fragments arranged by size: 

                 680     642      28
 
EcoRI G'AATT_C

Cuts at:      0     742     934    1350 
   Size:        742     192     416

  Fragments arranged by size: 

                 742     416     192
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     426    1350 
   Size:        426     924
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     885     917    1350 
   Size:        885      32     433

  Fragments arranged by size: 

                 885     433      32
 
HindIII A'AGCT_T

Cuts at:      0     598    1208    1350 
   Size:        598     610     142

  Fragments arranged by size: 

                 610     598     142
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     911     968    1350 
   Size:        911      57     382

  Fragments arranged by size: 

                 911     382      57
 
MscI TGG'CCA

Cuts at:      0     121    1137    1350 
   Size:        121    1016     213

  Fragments arranged by size: 

                1016     213     121
 
MspA1I CmG'CkG

Cuts at:      0     656    1350 
   Size:        656     694
 
NdeI CA'TA_TG

Cuts at:      0     792    1350 
   Size:        792     558
 
NsiI A_TGCA'T

Cuts at:      0      86     423     792    1350 
   Size:         86     337     369     558

  Fragments arranged by size: 

                 558     369     337      86
 
NspI r_CATG'y

Cuts at:      0     231     421    1350 
   Size:        231     190     929

  Fragments arranged by size: 

                 929     231     190
 
PciI A'CATG_T

Cuts at:      0     227    1350 
   Size:        227    1123
 
PfoI T'CCnGG_A

Cuts at:      0     696    1120    1350 
   Size:        696     424     230

  Fragments arranged by size: 

                 696     424     230
 
PpuMI rG'GwC_Cy

Cuts at:      0     708    1350 
   Size:        708     642
 
PstI C_TGCA'G

Cuts at:      0     222    1350 
   Size:        222    1128
 
PvuII CAG'CTG

Cuts at:      0     656    1350 
   Size:        656     694
 
SanDI GG'GwC_CC

Cuts at:      0     708    1350 
   Size:        708     642
 
SexAI A'CCwGG_T

Cuts at:      0     568    1350 
   Size:        568     782
 
SfcI C'TryA_G

Cuts at:      0     218    1350 
   Size:        218    1132
 
SmaI CCC'GGG

Cuts at:      0     514    1350 
   Size:        514     836
 
SmlI C'TyrA_G

Cuts at:      0     889    1350 
   Size:        889     461
 
SnaBI TAC'GTA

Cuts at:      0     108    1350 
   Size:        108    1242
 
SspI AAT'ATT

Cuts at:      0     381     521     675    1350 
   Size:        381     140     154     675

  Fragments arranged by size: 

                 675     381     154     140
 
StuI AGG'CCT

Cuts at:      0     541    1350 
   Size:        541     809
 
StyI C'CwwG_G

Cuts at:      0      91     248    1350 
   Size:         91     157    1102

  Fragments arranged by size: 

                1102     157      91
 
TatI w'GTAC_w

Cuts at:      0     230    1350 
   Size:        230    1120
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     480     512    1350 
   Size:        480      32     838

  Fragments arranged by size: 

                 838     480      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     339    1350 
   Size:        339    1011
 
XmaI C'CCGG_G

Cuts at:      0     512    1350 
   Size:        512     838
 
XmnI GAAnn'nnTTC

Cuts at:      0     803    1350 
   Size:        803     547
 

 Enzymes that do cut:

     AcuI    AflII   AflIII     AlfI     ApoI     AseI     AvaI     BanI
    BanII     BbsI    BfrBI    BglII     BmrI     BplI     BpmI    BsaAI
    BsaHI    BsaWI    BsaXI     BsgI  BsiHKAI     BsmI Bsp1286I    BspHI
    BsrGI    BstYI    CspCI     DraI     EaeI     EarI  Eco57MI    EcoNI
 EcoO109I    EcoRI     FalI    Hin4I  HindIII     MmeI     MscI   MspA1I
     NdeI     NsiI     NspI     PciI     PfoI    PpuMI     PstI    PvuII
    SanDI    SexAI     SfcI     SmaI     SmlI    SnaBI     SspI     StuI
     StyI     TatI     TstI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI     AgeI     AhdI
     AleI     AloI    AlwNI     ApaI    ApaLI     AscI    AsiSI    AvrII
     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI     BclI     BglI
     BlpI Bme1580I    BmgBI     BmtI   Bpu10I    BpuEI     BsaI    BsaBI
    BseRI    BseYI    BsiEI    BsiWI    BsmBI    BspEI    BspMI    BsrBI
    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI   DraIII     DrdI
     EagI     EciI  EcoICRI    EcoRV     FseI     FspI    FspAI    HaeII
   HincII     HpaI     KasI     KpnI     MfeI     MluI     MslI     NaeI
     NarI     NcoI   NgoMIV     NheI     NotI     NruI     PacI     PasI
    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI     SapI     SbfI
     ScaI     SfiI     SfoI     SgfI    SgrAI     SpeI     SphI     SrfI
     SwaI    TaqII  Tth111I     XbaI     XhoI     ZraI
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