Restriction map for pF1KB6875
##################################
# NO CUT ENZYMES (  160 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AflII       AflIII      AgeI        Alw44I      AlwI        
AocI        ApaI        ApaLI       AscI        AseI        
AsnI        AspHI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanII       BbeI        
BbrPI       BbvCI       BbvI        BcgI        BclI        
BfrI        BglI        BglII       BlnI        BlpI        
BmyI        Bpu1102I    BsaAI       BsaBI       BsaWI       
BseAI       BseRI       BsiEI       BsiHKAI     BsiWI       
BsmFI       BsmI        BsoBI       Bsp1286I    Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DraI        DraII       DraIII      
EaeI        EagI        EarI        EclXI       Eco52I      
Eco81I      EcoO109I    EcoRI       EcoRV       EcoT22I     
FauI        FbaI        FseI        FspI        HindIII     
KasI        Ksp632I     KspI        MaeII       MamI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MunI        MvnI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TaqI        TseI        Tth111I     TthHB8I     Van91I      
XbaI        XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   62 enzymes) #
###################################

Acc65I        165  GGTACC               G^GTACC
AcsI          454  AAATTC               [GA]^AATT[CT]
AcyI          439  GGCGTC               G[GA]^CG[CT]C
AfaI          166  GTAC                 GT^AC
AhdI          131  GACCAAATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI          60  CAGAATCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI       308  AGCGCT               AGC^GCT
ApoI          454  AAATTC               [GA]^AATT[CT]
Asp700I        81  GAAGACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       165  GGTACC               G^GTACC
AspEI         131  GACCAAATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII         130  GGACC                G^G[AT]CC
BanI          165  GGTACC               G^G[CT][GA]CC
BbsI           81  GAAGAC               GAAGAC(2/6)
BciVI         118  GGATAC               GTATCC(6/5)
BcnI          370  CCCGG                CC^[GC]GG
BfaI          109  CTAG                 C^TAG
BpuAI          81  GAAGAC               GAAGAC(2/6)
BsaHI         439  GGCGTC               G[GA]^CG[CT]C
BsaI          244  GAGACC               GGTCTC(1/5)
BsgI          384  CTGCAC               GTGCAG(16/14)
BsmBI          10  GAGACG               CGTCTC(1/5)
BsrBI         324  CCGCTC               CCGCTC(-3/-3)
BsrI          347  CCAGT                ACTGG(1/-1)
BstEII        447  GGTCACC              G^GT[ACGT]ACC
BstNI         465  CCAGG                CC^[AT]GG
BstPI         447  GGTCACC              G^GT[ACGT]ACC
CfoI          309  GCGC                 GCG^C
DpnII          15  GATC                 ^GATC
DsaI          201  CCATGG               C^C[GA][CT]GG
Eam1105I      131  GACCAAATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      308  AGCGCT               AGC^GCT
EcoNI         430  CCTGACGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       447  GGTCACC              G^GT[ACGT]ACC
EcoRII        465  CCAGG                ^CC[AT]GG
EcoT14I       201  CCATGG               C^C[AT][AT]GG
Fnu4HI        361  GCCGC                GC^[ACGT]GC
HaeII         308  AGCGCT               [GA]GCGC^[CT]
HaeIII        337  GGCC                 GG^CC
HgaI          440  GCGTC                GACGC(5/10)
HhaI          309  GCGC                 GCG^C
Hin1I         439  GGCGTC               G[GA]^CG[CT]C
HinP1I        309  GCGC                 G^CGC
ItaI          361  GCCGC                GC^[ACGT]GC
KpnI          165  GGTACC               GGTAC^C
MaeI          109  CTAG                 C^TAG
MaeIII        448  GTCAC                ^GT[ACGT]AC
MboI           15  GATC                 ^GATC
MspA1I        256  CAGCTG               C[AC]G^C[GT]G
MvaI          465  CCAGG                CC^[AT]GG
NciI          370  CCCGG                CC^[GC]GG
NcoI          201  CCATGG               C^CATGG
NdeII          15  GATC                 ^GATC
PvuII         256  CAGCTG               CAG^CTG
RsaI          166  GTAC                 GT^AC
Sau3AI         15  GATC                 ^GATC
SfaNI          48  GATGC                GCATC(5/9)
SpeI          108  ACTAGT               A^CTAGT
StyI          201  CCATGG               C^C[AT][AT]GG
Tsp45I        448  GTCAC                ^GT[GC]AC
XcmI          248  CCAGACTCCAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI           81  GAAGACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AciI          324  CCGC                 CCGC(-3/-1)
              362  CCGC                
AluI          257  AGCT                 AG^CT
              399  AGCT                
BpmI          253  CTCCAG               CTGGAG(16/14)
              259  CTGGAG              
BsaJI         201  CCATGG               C^C[ACGT][ACGT]GG
              369  CCCCGG              
BsiYI         255  CCAGCTGGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              430  CCTGACGAAGG         
BslI          255  CCAGCTGGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              430  CCTGACGAAGG         
BsmAI          10  GAGAC                GTCTC(1/5)
              244  GAGAC               
BssKI         370  CCCGG                ^CC[ACGT]GG
              465  CCAGG               
BstF5I        321  CATCC                GGATG(2/0)
               69  GGATG               
Cac8I          51  GCAAGC               GC[ACGT]^[ACGT]GC
              364  GCCTGC              
Cfr13I        130  GGACC                G^G[ACGT]CC
              337  GGCCC               
DrdI          403  GACCAGCCCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              433  GACGAAGGCGTC        
FokI          321  CATCC                GGATG(9/13)
               69  GGATG               
HapII         116  CCGG                 C^CGG
              371  CCGG                
HincII         73  GTTAAC               GT[CT]^[GA]AC
              268  GTTAAC              
HindII         73  GTTAAC               GT[CT]^[GA]AC
              268  GTTAAC              
HpaI           73  GTTAAC               GTT^AAC
              268  GTTAAC              
HpaII         116  CCGG                 C^CGG
              371  CCGG                
MseI           74  TTAA                 T^TAA
              269  TTAA                
MslI          387  CACAGCGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              420  CACCAATATG          
MspI          116  CCGG                 C^CGG
              371  CCGG                
MwoI          300  GCAGGACAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              391  GCGATGGAAGC         
PleI          251  GACTC                GAGTC(4/5)
              355  GAGTC               
Sau96I        130  GGACC                G^G[ACGT]CC
              337  GGCCC               
ScrFI         370  CCCGG                CC^[ACGT]GG
              465  CCAGG               
TfiI           62  GAATC                G^A[AT]TC
              197  GAATC               
Tru9I          74  TTAA                 T^TAA
              269  TTAA                
Tsp509I       142  AATT                 ^AATT
              455  AATT                
TspRI         276  CACTG                CA[GC]TG[ACGT][ACGT]^
              333  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (    7 enzymes) #
#########################################

DdeI        HinfI       HphI        MboII       MnlI        
NlaIII      NlaIV       
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