Restriction map for pF1KE1032
##################################
# NO CUT ENZYMES (  118 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AflII       AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       AscI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AviII       
BamHI       BanI        BbeI        BbrPI       BbvCI       
BcgI        BfrI        BglI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaI        BsaWI       BseAI       
BsiEI       BsiWI       BsoBI       BspDI       BspEI       
BspHI       BsrBI       BssHII      Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      CelII       CfoI        ClaI        
CpoI        DrdI        DsaI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoO65I     
EcoRV       FseI        FspI        HaeII       HhaI        
HinP1I      HpaI        KasI        KpnI        KspI        
MamI        MluI        MroI        MvnI        NarI        
NcoI        NotI        NruI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       Sse8387I    Tth111I     XbaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AcyI         2061  GACGCC               G[GA]^CG[CT]C
AvrII         894  CCTAGG               C^CTAGG
BalI          483  TGGCCA               TGG^CCA
BanII         329  GAGCCC               G[GA]GC[CT]^C
BciVI        2942  GTATCC               GTATCC(6/5)
BclI         1506  TGATCA               T^GATCA
BglII        3102  AGATCT               A^GATCT
BlnI          894  CCTAGG               C^CTAGG
BsaHI        2061  GACGCC               G[GA]^CG[CT]C
BsmBI        2211  CGTCTC               CGTCTC(1/5)
BsmI         3270  GAATGC               GAATGC(1/-1)
BspLU11I      917  ACATGT               A^CATGT
BsrFI        3407  GCCGGC               [GA]^CCGG[CT]
BssSI         746  CACGAG               CACGAG(-5/-1)
Cfr10I       3407  GCCGGC               [GA]^CCGG[CT]
DraII        3863  AGGCCCC              [GA]G^G[ACGT]CC[CT]
EaeI          483  TGGCCA               [CT]^GGCC[GA]
EcoO109I     3863  AGGCCCC              [GA]G^G[ACGT]CC[CT]
EcoT22I      2198  ATGCAT               ATGCA^T
FauI         1150  CCCGC                CCCGC(4/6)
FbaI         1506  TGATCA               T^GATCA
Hin1I        2061  GACGCC               G[GA]^CG[CT]C
MfeI          881  CAATTG               C^AATTG
MluNI         483  TGGCCA               TGG^CCA
MscI          483  TGGCCA               TGG^CCA
MunI          881  CAATTG               C^AATTG
NaeI         3407  GCCGGC               GCC^GGC
NdeI         2908  CATATG               CA^TATG
NgoMIV       3407  GCCGGC               G^CCGGC
NheI          786  GCTAGC               G^CTAGC
NsiI         2198  ATGCAT               ATGCA^T
SphI          293  GCATGC               GCATG^C
SspI          826  AATATT               AAT^ATT
StuI         1277  AGGCCT               AGG^CCT
SwaI         1841  ATTTAAAT             ATTT^AAAT
XcmI         1043  CCAGTCAACCTATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AccI          626  GTAGAC               GT^[AC][GT]AC
             4246  GTAGAC              
AflIII        917  ACATGT               A^C[GA][CT]GT
             3183  ACGTGT              
AspHI         584  GTGCTC               G[AT]GC[AT]^C
             4092  GAGCAC              
AvaII         267  GGACC                G^G[AT]CC
             3915  GGTCC               
BsgI         2681  CTGCAC               GTGCAG(16/14)
              401  GTGCAG              
BsiHKAI       584  GTGCTC               G[AT]GC[AT]^C
             4092  GAGCAC              
Bsp1407I      121  TGTACA               T^GTACA
             1159  TGTACA              
BsrDI        2496  CATTGC               GCAATG(2/0)
             4265  CATTGC              
BsrGI         121  TGTACA               T^GTACA
             1159  TGTACA              
DraIII       1394  CACATTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2519  CACCATGTG           
HgaI          629  GACGC                GACGC(5/10)
             2061  GACGC               
PflMI        2826  CCAAGGAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3240  CCATAAGATGG         
PstI         2465  CTGCAG               CTGCA^G
             3945  CTGCAG              
SpeI          814  ACTAGT               A^CTAGT
             2355  ACTAGT              
SspBI         121  TGTACA               T^GTACA
             1159  TGTACA              
Van91I       2826  CCAAGGAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3240  CCATAAGATGG         


#########################################
# 3 OR MORE CUT ENZYMES (   88 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        AseI        AsnI        BbsI        
BbvI        BcnI        BfaI        BmyI        BpmI        
BpuAI       BsaJI       BseRI       BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BspMI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
Cfr13I      DdeI        DpnII       DraI        EarI        
EcoNI       EcoRI       EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HapII       HincII      HindII      
HindIII     HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PshBI       PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII       
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